################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FokI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 1198 CGCG CG^CG AcyI 848 GGCGCC G[GA]^CG[CT]C Aor51HI 458 AGCGCT AGC^GCT ApaI 1202 GGGCCC GGGCC^C BbeI 848 GGCGCC GGCGC^C BclI 275 TGATCA T^GATCA BlpI 599 GCTCAGC GC^T[ACGT]AGC Bpu1102I 599 GCTCAGC GC^T[ACGT]AGC BsaHI 848 GGCGCC G[GA]^CG[CT]C BsaI 149 GAGACC GGTCTC(1/5) BsmI 205 GAATGC GAATGC(1/-1) BsrDI 48 CATTGC GCAATG(2/0) BstUI 1198 CGCG CG^CG BstXI 561 CCACCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 599 GCTCAGC GC^T[ACGT]AGC DpnII 276 GATC ^GATC DraIII 646 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 458 AGCGCT AGC^GCT EcoNI 1146 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 275 TGATCA T^GATCA Hin1I 848 GGCGCC G[GA]^CG[CT]C KasI 848 GGCGCC G^GCGCC KspI 1197 CCGCGG CCGC^GG MboI 276 GATC ^GATC MvnI 1198 CGCG CG^CG NarI 848 GGCGCC GG^CGCC NdeII 276 GATC ^GATC NspI 52 GCATGT [GA]CATG^[CT] SacII 1197 CCGCGG CCGC^GG Sau3AI 276 GATC ^GATC SfoI 848 GGCGCC GGC^GCC StuI 874 AGGCCT AGG^CCT TaqI 1088 TCGA T^CGA Tsp509I 126 AATT ^AATT TthHB8I 1088 TCGA T^CGA XbaI 882 TCTAGA T^CTAGA XcmI 558 CCACCACCGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 189 GAGCAC G[AT]GC[AT]^C 526 GTGCTC AvaI 32 CCCGGG C^[CT]CG[GA]G 515 CCCGGG BfaI 326 CTAG C^TAG 883 CTAG BglI 892 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1031 GCCCAGGGGGC BpmI 777 CTCCAG CTGGAG(16/14) 630 CTGGAG BsgI 76 CTGCAC GTGCAG(16/14) 643 CTGCAC BsiHKAI 189 GAGCAC G[AT]GC[AT]^C 526 GTGCTC BsmBI 429 GAGACG CGTCTC(1/5) 771 GAGACG BsoBI 32 CCCGGG C^[CT]CG[GA]G 515 CCCGGG BsrFI 662 GCCGGC [GA]^CCGG[CT] 1176 GCCGGC Cfr10I 662 GCCGGC [GA]^CCGG[CT] 1176 GCCGGC DrdI 823 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1093 GACCACCTCGTC EarI 170 CTCTTC CTCTTC(1/4) 1073 CTCTTC EcoT14I 395 CCTTGG C^C[AT][AT]GG 585 CCAAGG Ksp632I 170 CTCTTC CTCTTC(1/4) 1073 CTCTTC MaeI 326 CTAG C^TAG 883 CTAG MaeII 524 ACGT A^CGT 986 ACGT MseI 818 TTAA T^TAA 839 TTAA NaeI 662 GCCGGC GCC^GGC 1176 GCCGGC NgoMIV 662 GCCGGC G^CCGGC 1176 GCCGGC PshAI 955 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1056 GACCCCGGTC PstI 673 CTGCAG CTGCA^G 1181 CTGCAG PvuII 930 CAGCTG CAG^CTG 1137 CAGCTG SfaNI 197 GCATC GCATC(5/9) 290 GCATC SmaI 32 CCCGGG CCC^GGG 515 CCCGGG StyI 395 CCTTGG C^C[AT][AT]GG 585 CCAAGG Tru9I 818 TTAA T^TAA 839 TTAA XmaCI 32 CCCGGG C^CCGGG 515 CCCGGG XmaI 32 CCCGGG C^CCGGG 515 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BanII BbvCI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflMI PleI PpuMI Sau96I ScrFI SfcI TseI Tsp45I TspRI Van91I