Restriction map for pF1KSDA0139
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
Alw44I      ApaLI       AscI        Asp718I     AviII       
BalI        BbeI        BbrPI       BcgI        BciVI       
BfrI        BlpI        Bpu1102I    BsaAI       BseAI       
BsiEI       Bsp1407I    BspEI       BspHI       BspLU11I    
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       CpoI        DraI        
DraIII      EaeI        EagI        EclXI       Eco52I      
EcoO65I     EcoRV       FseI        FspI        HpaI        
KasI        KpnI        KspI        MluNI       MroI        
MscI        NarI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          954  GTCTAC               GT^[AC][GT]AC
AclI         2105  AACGTT               AA^CGTT
AflIII        565  ACGCGT               A^C[GA][CT]GT
AhdI         3509  GACCATTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        2714  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2914  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2134  AGCGCT               AGC^GCT
ApaI         3411  GGGCCC               GGGCC^C
AseI         1111  ATTAAT               AT^TAAT
AsnI         1111  ATTAAT               AT^TAAT
AspEI        3509  GACCATTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1690  GAGCAC               G[AT]GC[AT]^C
AvrII        3724  CCTAGG               C^CTAGG
BclI         1452  TGATCA               T^GATCA
BglI         2958  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3861  AGATCT               A^GATCT
BlnI         3724  CCTAGG               C^CTAGG
BsaWI        1263  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1690  GAGCAC               G[AT]GC[AT]^C
BsiWI        1003  CGTACG               C^GTACG
BspDI        2366  ATCGAT               AT^CGAT
BsrDI        1429  CATTGC               GCAATG(2/0)
BssHII       2490  GCGCGC               G^CGCGC
Bsu36I       2914  CCTGAGG              CC^T[ACGT]AGG
ClaI         2366  ATCGAT               AT^CGAT
DrdI         1569  GACAGCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          442  CCATGG               C^C[GA][CT]GG
Eam1105I     3509  GACCATTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2134  AGCGCT               AGC^GCT
Eco81I       2914  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3052  CCTAGACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1452  TGATCA               T^GATCA
HincII        233  GTCAAC               GT[CT]^[GA]AC
HindII        233  GTCAAC               GT[CT]^[GA]AC
HindIII       539  AAGCTT               A^AGCTT
MluI          565  ACGCGT               A^CGCGT
MslI         3031  CATGCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI            3  GCCGGC               GCC^GGC
NcoI          442  CCATGG               C^CATGG
NgoMIV          3  GCCGGC               G^CCGGC
NspI         1538  GCATGC               [GA]CATG^[CT]
PflMI        3112  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1111  ATTAAT               AT^TAAT
Psp1406I     2105  AACGTT               AA^CGTT
PstI         1318  CTGCAG               CTGCA^G
ScaI         1584  AGTACT               AGT^ACT
SexAI        3495  ACCTGGT              A^CC[AT]GGT
SphI         1538  GCATGC               GCATG^C
Van91I       3112  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI         2813  GGCGTC               G[GA]^CG[CT]C
             3026  GGCGTC              
AspI         2906  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3494  GACCTGGTC           
AvaI         1502  CTCGAG               C^[CT]CG[GA]G
             3575  CTCGAG              
BamHI        1700  GGATCC               G^GATCC
             1992  GGATCC              
BanI         1945  GGTGCC               G^G[CT][GA]CC
             2689  GGCACC              
BanII        2839  GAGCCC               G[GA]GC[CT]^C
             3411  GGGCCC              
BbvCI        1819  GCTGAGG              CCTCAGC(-5/-2)
             3757  GCTGAGG             
BsaHI        2813  GGCGTC               G[GA]^CG[CT]C
             3026  GGCGTC              
BsgI         2297  CTGCAC               GTGCAG(16/14)
             3395  GTGCAG              
BsoBI        1502  CTCGAG               C^[CT]CG[GA]G
             3575  CTCGAG              
BspMI        1323  GCAGGT               ACCTGC(4/8)
             1440  GCAGGT              
BstAPI       1275  GCAGTATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1533  GCAAAGCATGC         
EcoRI         577  GAATTC               G^AATTC
             3380  GAATTC              
EcoT22I       682  ATGCAT               ATGCA^T
              875  ATGCAT              
Hin1I        2813  GGCGTC               G[GA]^CG[CT]C
             3026  GGCGTC              
MfeI         1173  CAATTG               C^AATTG
             1721  CAATTG              
MunI         1173  CAATTG               C^AATTG
             1721  CAATTG              
NsiI          682  ATGCAT               ATGCA^T
              875  ATGCAT              
PaeR7I       1502  CTCGAG               C^TCGAG
             3575  CTCGAG              
PflFI        2906  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3494  GACCTGGTC           
SfcI         1318  CTGCAG               C^T[GA][CT]AG
             2418  CTATAG              
SspI          358  AATATT               AAT^ATT
             1756  AATATT              
StuI         2986  AGGCCT               AGG^CCT
             4058  AGGCCT              
Tth111I      2906  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3494  GACCTGGTC           
XhoI         1502  CTCGAG               C^TCGAG
             3575  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        Bsp1286I    BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PshAI       PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XcmI        XhoII       XmnI        
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