Restriction map for pF1KSDA0139
 (Linear) (Six-base) MAPSORT of: KIAA0139.seq  Check: 3856  from: 1  to: 4146



KIAA0139.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1314    4146 
   Size:       1314    2832
 
AccI GT'mk_AC

Cuts at:      0     955    4146 
   Size:        955    3191
 
AclI AA'CG_TT

Cuts at:      0    2106    4146 
   Size:       2106    2040
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323    1529    2729    3699    4146 
   Size:        323    1206    1200     970     447

  Fragments arranged by size: 

                1206    1200     970     447     323
 
AfeI AGC'GCT

Cuts at:      0    2136    4146 
   Size:       2136    2010
 
AflIII A'CryG_T

Cuts at:      0     565    4146 
   Size:        565    3581
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3514    4146 
   Size:       3514     632
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2911    2943    4146 
   Size:       2911      32    1203

  Fragments arranged by size: 

                2911    1203      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2719    4146 
   Size:       2719    1427
 
ApaI G_GGCC'C

Cuts at:      0    3415    4146 
   Size:       3415     731
 
ApoI r'AATT_y

Cuts at:      0      49     451     547     577    1189    1677    2117    3380 
   Size:         49     402      96      30     612     488     440    1263

Cuts at:   3380    4146 
   Size:        766

  Fragments arranged by size: 

                1263     766     612     488     440     402      96      49
                  30
 
AseI AT'TA_AT

Cuts at:      0    1112    4146 
   Size:       1112    3034
 
AvaI C'yCGr_G

Cuts at:      0    1502    3575    4146 
   Size:       1502    2073     571

  Fragments arranged by size: 

                2073    1502     571
 
AvrII C'CTAG_G

Cuts at:      0    3724    4146 
   Size:       3724     422
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2512    2545    4146 
   Size:       2512      33    1601

  Fragments arranged by size: 

                2512    1601      33
 
BamHI G'GATC_C

Cuts at:      0    1700    1992    4146 
   Size:       1700     292    2154

  Fragments arranged by size: 

                2154    1700     292
 
BanI G'GyrC_C

Cuts at:      0    1945    2689    4146 
   Size:       1945     744    1457

  Fragments arranged by size: 

                1945    1457     744
 
BanII G_rGCy'C

Cuts at:      0    2843    3415    4146 
   Size:       2843     572     731

  Fragments arranged by size: 

                2843     731     572
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     410    1072    1982    2270    2634    3017    3726    4146 
   Size:        410     662     910     288     364     383     709     420

  Fragments arranged by size: 

                 910     709     662     420     410     383     364     288
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1820    3758    4146 
   Size:       1820    1938     388

  Fragments arranged by size: 

                1938    1820     388
 
BclI T'GATC_A

Cuts at:      0    1452    4146 
   Size:       1452    2694
 
BfrBI ATG'CAT

Cuts at:      0     684     877    4146 
   Size:        684     193    3269

  Fragments arranged by size: 

                3269     684     193
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2964    4146 
   Size:       2964    1182
 
BglII A'GATC_T

Cuts at:      0    3861    4146 
   Size:       3861     285
 
Bme1580I G_kGCm'C

Cuts at:      0    3415    4146 
   Size:       3415     731
 
BmgBI CAC'GTC

Cuts at:      0    2758    2908    3118    3451    3934    4146 
   Size:       2758     150     210     333     483     212

  Fragments arranged by size: 

                2758     483     333     212     210     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1951    4146 
   Size:       1951    2195
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4077    4146 
   Size:       4077      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277    1786    2050    2715    3762    3795    4146 
   Size:        277    1509     264     665    1047      33     351

  Fragments arranged by size: 

                1509    1047     665     351     277     264      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     998    1820    3758    4146 
   Size:        998     822    1938     388

  Fragments arranged by size: 

                1938     998     822     388
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76    1765    2002    3474    3768    4146 
   Size:         76    1689     237    1472     294     378

  Fragments arranged by size: 

                1689    1472     378     294     237      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2789    3501    4004    4016    4022    4146 
   Size:       2789     712     503      12       6     124

  Fragments arranged by size: 

                2789     712     503     124      12       6
 
BsaBI GATnn'nnATC

Cuts at:      0    3224    3344    3434    3461    3698    4146 
   Size:       3224     120      90      27     237     448

  Fragments arranged by size: 

                3224     448     237     120      90      27
 
BsaHI Gr'CG_yC

Cuts at:      0    2814    3027    4146 
   Size:       2814     213    1119

  Fragments arranged by size: 

                2814    1119     213
 
BsaWI w'CCGG_w

Cuts at:      0    1263    4146 
   Size:       1263    2883
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1741    2449    2452    3164    3571    3664    3968    4146 
   Size:       1741     708       3     712     407      93     304     178

  Fragments arranged by size: 

                1741     712     708     407     304     178      93       3
            
 
BseYI C'CCAG_C

Cuts at:      0     533    3563    3996    4146 
   Size:        533    3030     433     150

  Fragments arranged by size: 

                3030     533     433     150
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2282    3416    4146 
   Size:       2282    1134     730

  Fragments arranged by size: 

                2282    1134     730
 
BsiHKAI G_wGCw'C

Cuts at:      0    1694    4146 
   Size:       1694    2452
 
BsiWI C'GTAC_G

Cuts at:      0    1003    4146 
   Size:       1003    3143
 
BsmI GAATG_Cn'

Cuts at:      0     611     745    1954    2261    4146 
   Size:        611     134    1209     307    1885

  Fragments arranged by size: 

                1885    1209     611     307     134
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2748    3863    3924    4146 
   Size:       2748    1115      61     222

  Fragments arranged by size: 

                2748    1115     222      61
 
Bsp1286I G_dGCh'C

Cuts at:      0    1694    2843    3415    4146 
   Size:       1694    1149     572     731

  Fragments arranged by size: 

                1694    1149     731     572
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1314    1431    4146 
   Size:       1314     117    2715

  Fragments arranged by size: 

                2715    1314     117
 
BsrBI CCG'CTC

Cuts at:      0    2475    2538    2805    3969    4146 
   Size:       2475      63     267    1164     177

  Fragments arranged by size: 

                2475    1164     267     177      63
 
BsrDI GCAATG_nn'

Cuts at:      0    1428    4146 
   Size:       1428    2718
 
BsrFI r'CCGG_y

Cuts at:      0       3    2954    3836    4146 
   Size:          3    2951     882     310

  Fragments arranged by size: 

                2951     882     310       3
 
BssHII G'CGCG_C

Cuts at:      0    2490    4146 
   Size:       2490    1656
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1281    1539    4146 
   Size:       1281     258    2607

  Fragments arranged by size: 

                2607    1281     258
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     687    2109    3530    4146 
   Size:        687    1422    1421     616

  Fragments arranged by size: 

                1422    1421     687     616
 
BstYI r'GATC_y

Cuts at:      0     177     348    1480    1700    1992    2166    3861    4146 
   Size:        177     171    1132     220     292     174    1695     285

  Fragments arranged by size: 

                1695    1132     292     285     220     177     174     171
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2915    4146 
   Size:       2915    1231
 
BtgI C'CryG_G

Cuts at:      0     442    4146 
   Size:        442    3704
 
BtsI GCAGTG_nn'

Cuts at:      0     481    1661    4146 
   Size:        481    1180    2485

  Fragments arranged by size: 

                2485    1180     481
 
ClaI AT'CG_AT

Cuts at:      0    2367    4146 
   Size:       2367    1779
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3507    3542    4146 
   Size:       3507      35     604

  Fragments arranged by size: 

                3507     604      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1575    4146 
   Size:       1575    2571
 
EarI CTCTTCn'nnn_

Cuts at:      0    1820    2333    2426    2474    2510    2615    2724    2787 
   Size:       1820     513      93      48      36     105     109      63

Cuts at:   2787    2795    3741    3879    3959    4146 
   Size:          8     946     138      80     187

  Fragments arranged by size: 

                1820     946     513     187     138     109     105      93
                  80      63      48      36       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1300    4146 
   Size:       1300    2846
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     323    1529    1786    2050    2715    2729    3699 
   Size:        277      46    1206     257     264     665      14     970

Cuts at:   3699    3762    3795    4146 
   Size:         63      33     351

  Fragments arranged by size: 

                1206     970     665     351     277     264     257      63
                  46      33      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3056    4146 
   Size:       3056    1090
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2704    2899    3202    3232    3262    3292    3322    3352 
   Size:       2704     195     303      30      30      30      30      30

Cuts at:   3352    3411    3412    3663    3889    3972    4146 
   Size:         59       1     251     226      83     174

  Fragments arranged by size: 

                2704     303     251     226     195     174      83      59
                  30      30      30      30      30       1
 
EcoRI G'AATT_C

Cuts at:      0     577    3380    4146 
   Size:        577    2803     766

  Fragments arranged by size: 

                2803     766     577
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1559    1759    4146 
   Size:        210    1349     200    2387

  Fragments arranged by size: 

                2387    1349     210     200
 
HaeII r_GCGC'y

Cuts at:      0     516     630    1743    2138    3158    4146 
   Size:        516     114    1113     395    1020     988

  Fragments arranged by size: 

                1113    1020     988     516     395     114
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     661     693     909     941    1509    1541    1692    1724 
   Size:        661      32     216      32     568      32     151      32

Cuts at:   1724    2767    2799    2914    2946    3154    3157    3186    3189 
   Size:       1043      32     115      32     208       3      29       3

Cuts at:   3189    3500    3532    3552    3584    3881    3913    4072    4104 
   Size:        311      32      20      32     297      32     159      32

Cuts at:   4104    4146 
   Size:         42

  Fragments arranged by size: 

                1043     661     568     311     297     216     208     159
                 151     115      42      32      32      32      32      32
                  32      32      32      32      32      29      20       3
                   3
 
HincII GTy'rAC

Cuts at:      0     235    4146 
   Size:        235    3911
 
HindIII A'AGCT_T

Cuts at:      0     539    4146 
   Size:        539    3607
 
MfeI C'AATT_G

Cuts at:      0    1173    1721    4146 
   Size:       1173     548    2425

  Fragments arranged by size: 

                2425    1173     548
 
MluI A'CGCG_T

Cuts at:      0     565    4146 
   Size:        565    3581
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1419    2567    3197    3317    4146 
   Size:       1419    1148     630     120     829

  Fragments arranged by size: 

                1419    1148     829     630     120
 
MslI CAynn'nnrTG

Cuts at:      0    3035    4146 
   Size:       3035    1111
 
MspA1I CmG'CkG

Cuts at:      0     708    1566    1698    3094    3757    4146 
   Size:        708     858     132    1396     663     389

  Fragments arranged by size: 

                1396     858     708     663     389     132
 
NaeI GCC'GGC

Cuts at:      0       5    4146 
   Size:          5    4141
 
NcoI C'CATG_G

Cuts at:      0     442    4146 
   Size:        442    3704
 
NgoMIV G'CCGG_C

Cuts at:      0       3    4146 
   Size:          3    4143
 
NsiI A_TGCA'T

Cuts at:      0     686     879    4146 
   Size:        686     193    3267

  Fragments arranged by size: 

                3267     686     193
 
NspI r_CATG'y

Cuts at:      0    1542    4146 
   Size:       1542    2604
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3118    4146 
   Size:       3118    1028
 
PpuMI rG'GwC_Cy

Cuts at:      0    2704    3202    3232    3262    3292    3322    3352    3663 
   Size:       2704     498      30      30      30      30      30     311

Cuts at:   3663    3889    3972    4146 
   Size:        226      83     174

  Fragments arranged by size: 

                2704     498     311     226     174      83      30      30
                  30      30      30
 
PshAI GACnn'nnGTC

Cuts at:      0    3200    3260    3290    3320    3785    4146 
   Size:       3200      60      30      30     465     361

  Fragments arranged by size: 

                3200     465     361      60      30      30
 
PspOMI G'GGCC_C

Cuts at:      0    3411    4146 
   Size:       3411     735
 
PstI C_TGCA'G

Cuts at:      0    1322    4146 
   Size:       1322    2824
 
PvuII CAG'CTG

Cuts at:      0     708    1566    3094    3757    4146 
   Size:        708     858    1528     663     389

  Fragments arranged by size: 

                1528     858     708     663     389
 
SanDI GG'GwC_CC

Cuts at:      0    3202    3232    3262    3292    3322    3972    4146 
   Size:       3202      30      30      30      30     650     174

  Fragments arranged by size: 

                3202     650     174      30      30      30      30
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2333    2510    2795    3959    4146 
   Size:       2333     177     285    1164     187

  Fragments arranged by size: 

                2333    1164     285     187     177
 
ScaI AGT'ACT

Cuts at:      0    1586    4146 
   Size:       1586    2560
 
SexAI A'CCwGG_T

Cuts at:      0    3495    4146 
   Size:       3495     651
 
SfcI C'TryA_G

Cuts at:      0    1318    2418    4146 
   Size:       1318    1100    1728

  Fragments arranged by size: 

                1728    1318    1100
 
SmlI C'TyrA_G

Cuts at:      0      55    1502    1744    1981    3489    3575    3783    4146 
   Size:         55    1447     242     237    1508      86     208     363

  Fragments arranged by size: 

                1508    1447     363     242     237     208      86      55
            
 
SphI G_CATG'C

Cuts at:      0    1542    4146 
   Size:       1542    2604
 
SspI AAT'ATT

Cuts at:      0     360    1758    4146 
   Size:        360    1398    2388

  Fragments arranged by size: 

                2388    1398     360
 
StuI AGG'CCT

Cuts at:      0    2988    4060    4146 
   Size:       2988    1072      86

  Fragments arranged by size: 

                2988    1072      86
 
StyI C'CwwG_G

Cuts at:      0     442    3355    3415    3502    3724    4146 
   Size:        442    2913      60      87     222     422

  Fragments arranged by size: 

                2913     442     422     222      87      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1933    3896    4028    4040    4046    4146 
   Size:       1933    1963     132      12       6     100

  Fragments arranged by size: 

                1963    1933     132     100      12       6
 
TatI w'GTAC_w

Cuts at:      0    1134    1584    4146 
   Size:       1134     450    2562

  Fragments arranged by size: 

                2562    1134     450
 
Tth111I GACn'n_nGTC

Cuts at:      0    2909    3497    4146 
   Size:       2909     588     649

  Fragments arranged by size: 

                2909     649     588
 
XbaI T'CTAG_A

Cuts at:      0     351     502     907    2650    4146 
   Size:        351     151     405    1743    1496

  Fragments arranged by size: 

                1743    1496     405     351     151
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2747    3213    3243    3273    3303    3333    4146 
   Size:       2747     466      30      30      30      30     813

  Fragments arranged by size: 

                2747     813     466      30      30      30      30
 
XhoI C'TCGA_G

Cuts at:      0    1502    3575    4146 
   Size:       1502    2073     571

  Fragments arranged by size: 

                2073    1502     571
 
XmnI GAAnn'nnTTC

Cuts at:      0     498     764    2117    4146 
   Size:        498     266    1353    2029

  Fragments arranged by size: 

                2029    1353     498     266
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI   AflIII     AhdI     AloI
    AlwNI     ApaI     ApoI     AseI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DrdI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII  HindIII     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI    PflMI    PpuMI
    PshAI   PspOMI     PstI    PvuII    SanDI     SapI     ScaI    SexAI
     SfcI     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII     AgeI     AleI     AlfI    ApaLI     AscI
    AsiSI     BbeI     BcgI    BciVI     BlpI     BmtI    BsaAI    BsaXI
    BsiEI    BspEI    BspHI    BsrGI    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     DraI   DraIII     EaeI     EagI  EcoICRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MscI     NarI     NdeI
     NheI     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SwaI     TstI     XmaI     ZraI
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