Restriction map for pF1KSDA0137
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflIII      AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BseAI       
BsgI        BsiWI       BsmI        BspEI       BspLU11I    
BspMI       BsrBI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AclI         1265  AACGTT               AA^CGTT
AflII        1759  CTTAAG               C^TTAAG
AgeI         2100  ACCGGT               A^CCGGT
Alw44I       2173  GTGCAC               G^TGCAC
AlwNI         688  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        2173  GTGCAC               G^TGCAC
BalI          426  TGGCCA               TGG^CCA
BamHI         165  GGATCC               G^GATCC
BanI         1074  GGCACC               G^G[CT][GA]CC
BanII          75  GGGCTC               G[GA]GC[CT]^C
BciVI        1587  GGATAC               GTATCC(6/5)
BfrI         1759  CTTAAG               C^TTAAG
BsaAI        1295  TACGTG               [CT]AC^GT[GA]
BsaBI        1639  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         509  CTCCTC               GAGGAG(10/8)
BsiEI         553  CGACCG               CG[GA][CT]^CG
BsmBI          62  CGTCTC               CGTCTC(1/5)
BspHI        1188  TCATGA               T^CATGA
BsrFI        2100  ACCGGT               [GA]^CCGG[CT]
BstXI        1832  CCAAGATTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          926  GCGC                 GCG^C
Cfr10I       2100  ACCGGT               [GA]^CCGG[CT]
DraI          625  TTTAAA               TTT^AAA
EcoRV        1718  GATATC               GAT^ATC
HaeII         925  GGCGCT               [GA]GCGC^[CT]
HgaI         2135  GACGC                GACGC(5/10)
HhaI          926  GCGC                 GCG^C
HinP1I        926  GCGC                 G^CGC
HincII       1170  GTTGAC               GT[CT]^[GA]AC
HindII       1170  GTTGAC               GT[CT]^[GA]AC
HindIII       297  AAGCTT               A^AGCTT
MamI         1639  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         426  TGGCCA               TGG^CCA
MscI          426  TGGCCA               TGG^CCA
MspA1I       2179  CAGCTG               C[AC]G^C[GT]G
MwoI           82  GCGGCGGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         687  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2100  ACCGGT               A^CCGGT
Psp1406I     1265  AACGTT               AA^CGTT
PstI         1515  CTGCAG               CTGCA^G
PvuII        2179  CAGCTG               CAG^CTG
RcaI         1188  TCATGA               T^CATGA
SfaNI        2260  GCATC                GCATC(5/9)
SfcI         1515  CTGCAG               C^T[GA][CT]AG
SmaI           72  CCCGGG               CCC^GGG
SspI         2064  AATATT               AAT^ATT
Van91I        687  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          72  CCCGGG               C^CCGGG
XmaI           72  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AspHI         219  GAGCAC               G[AT]GC[AT]^C
             2173  GTGCAC              
AvaI           72  CCCGGG               C^[CT]CG[GA]G
              898  CTCGGG              
BbvI          664  GCAGC                GCAGC(8/12)
             1444  GCTGC               
BpmI          203  CTGGAG               CTGGAG(16/14)
             1476  CTGGAG              
BsaWI         333  ACCGGA               [AT]^CCGG[AT]
             2100  ACCGGT              
BsiHKAI       219  GAGCAC               G[AT]GC[AT]^C
             2173  GTGCAC              
BsmAI         328  GAGAC                GTCTC(1/5)
               63  GTCTC               
BsoBI          72  CCCGGG               C^[CT]CG[GA]G
              898  CTCGGG              
Bsp1407I      603  TGTACA               T^GTACA
             1603  TGTACA              
BspDI         887  ATCGAT               AT^CGAT
             2162  ATCGAT              
BsrDI         142  GCAATG               GCAATG(2/0)
             1628  GCAATG              
BsrGI         603  TGTACA               T^GTACA
             1603  TGTACA              
ClaI          887  ATCGAT               AT^CGAT
             2162  ATCGAT              
DraII          94  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              689  AGGACCT             
EaeI           89  CGGCCA               [CT]^GGCC[GA]
              426  TGGCCA              
EarI          873  GAAGAG               CTCTTC(1/4)
             1237  GAAGAG              
EcoO109I       94  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              689  AGGACCT             
EcoT14I      1583  CCTTGG               C^C[AT][AT]GG
             2120  CCAAGG              
Ksp632I       873  GAAGAG               CTCTTC(1/4)
             1237  GAAGAG              
MaeIII        314  GTGAC                ^GT[ACGT]AC
             2024  GTCAC               
NspI         1509  ACATGC               [GA]CATG^[CT]
             1795  GCATGT              
PleI         1277  GAGTC                GAGTC(4/5)
             1982  GAGTC               
PpuMI          94  AGGTCCC              [GA]G^G[AT]CC[CT]
              689  AGGACCT             
SmlI          471  CTCAAG               C^T[CT][GA]AG
             1759  CTTAAG              
SspBI         603  TGTACA               T^GTACA
             1603  TGTACA              
StyI         1583  CCTTGG               C^C[AT][AT]GG
             2120  CCAAGG              
TseI          664  GCAGC                G^C[AT]GC
             1444  GCTGC               
Tsp45I        314  GTGAC                ^GT[GC]AC
             2024  GTCAC               
XcmI            9  CCAAAGTAGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              456  CCAGGGTGGAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BcnI        BfaI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TaqI        TfiI        Tru9I       Tsp509I     
TspRI       TthHB8I     XhoII       
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