Restriction map for pF1KSDA0132
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BfrI        BlnI        BsaAI       
BsaBI       BsaI        BseAI       BsiWI       BsmI        
Bsp1407I    BspEI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CpoI        
DraI        Eam1105I    EarI        EcoNI       EcoRV       
FseI        FspI        HindIII     HpaI        KpnI        
Ksp632I     MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        ScaI        SfiI        SfuI        SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         232  GACGTC               GACGT^C
AcsI          412  GAATTC               [GA]^AATT[CT]
AflIII       1724  ACACGT               A^C[GA][CT]GT
AocI           70  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1604  AGCGCT               AGC^GCT
ApoI          412  GAATTC               [GA]^AATT[CT]
AspHI         919  GAGCTC               G[AT]GC[AT]^C
AvaI          608  CCCGGG               C^[CT]CG[GA]G
BbeI          960  GGCGCC               GGCGC^C
BciVI         380  GTATCC               GTATCC(6/5)
BclI         1496  TGATCA               T^GATCA
BfaI           20  CTAG                 C^TAG
BglII         908  AGATCT               A^GATCT
BsaWI        1058  TCCGGT               [AT]^CCGG[AT]
BsiEI         266  CGGCCG               CG[GA][CT]^CG
BsiHKAI       919  GAGCTC               G[AT]GC[AT]^C
BsmAI        1624  GAGAC                GTCTC(1/5)
BsmBI        1624  GAGACG               CGTCTC(1/5)
BsoBI         608  CCCGGG               C^[CT]CG[GA]G
BspDI        1261  ATCGAT               AT^CGAT
BstAPI        942  GCAGGTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         70  CCTGAGG              CC^T[ACGT]AGG
ClaI         1261  ATCGAT               AT^CGAT
DraII          31  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EagI          266  CGGCCG               C^GGCCG
EclXI         266  CGGCCG               C^GGCCG
Eco47III     1604  AGCGCT               AGC^GCT
Eco52I        266  CGGCCG               C^GGCCG
Eco81I         70  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       31  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         412  GAATTC               G^AATTC
EcoT22I       625  ATGCAT               ATGCA^T
FbaI         1496  TGATCA               T^GATCA
HincII        825  GTTGAC               GT[CT]^[GA]AC
HindII        825  GTTGAC               GT[CT]^[GA]AC
KasI          960  GGCGCC               G^GCGCC
MaeI           20  CTAG                 C^TAG
MseI         1451  TTAA                 T^TAA
NaeI         1096  GCCGGC               GCC^GGC
NarI          960  GGCGCC               GG^CGCC
NgoMIV       1096  GCCGGC               G^CCGGC
NsiI          625  ATGCAT               ATGCA^T
NspI          623  ACATGC               [GA]CATG^[CT]
SacI          919  GAGCTC               GAGCT^C
SbfI         1071  CCTGCAGG             CCTGCA^GG
SexAI         899  ACCTGGT              A^CC[AT]GGT
SfoI          960  GGCGCC               GGC^GCC
SgrAI        1654  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          608  CCCGGG               CCC^GGG
Sse8387I     1071  CCTGCAGG             CCTGCA^GG
Tru9I        1451  TTAA                 T^TAA
XmaCI         608  CCCGGG               C^CCGGG
XmaI          608  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BalI          641  TGGCCA               TGG^CCA
             1266  TGGCCA              
BanII         919  GAGCTC               G[GA]GC[CT]^C
             1831  GAGCCC              
BbsI          316  GTCTTC               GAAGAC(2/6)
              733  GTCTTC              
BcgI          833  CGAACTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              545  GCAATGCCATCG        
BglI          418  GCCTACACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1091  GCCTGGCCGGC         
BpuAI         316  GTCTTC               GAAGAC(2/6)
              733  GTCTTC              
BseRI         652  GAGGAG               GAGGAG(10/8)
              916  GAGGAG              
BspHI         197  TCATGA               T^CATGA
              464  TCATGA              
BsrBI        1086  GAGCGG               CCGCTC(-3/-3)
             1336  GAGCGG              
BsrDI         545  GCAATG               GCAATG(2/0)
             1504  GCAATG              
BstEII        684  GGTGACC              G^GT[ACGT]ACC
             1779  GGTGACC             
BstPI         684  GGTGACC              G^GT[ACGT]ACC
             1779  GGTGACC             
DrdI          493  GACAGCGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              895  GACTACCTGGTC        
EcoO65I       684  GGTGACC              G^GT[ACGT]ACC
             1779  GGTGACC             
KspI          989  CCGCGG               CCGC^GG
             1081  CCGCGG              
MluNI         641  TGGCCA               TGG^CCA
             1266  TGGCCA              
MscI          641  TGGCCA               TGG^CCA
             1266  TGGCCA              
NcoI          437  CCATGG               C^CATGG
             1826  CCATGG              
RcaI          197  TCATGA               T^CATGA
              464  TCATGA              
SacII         989  CCGCGG               CCGC^GG
             1081  CCGCGG              
StuI           16  AGGCCT               AGG^CCT
              185  AGGCCT              
TfiI           44  GATTC                G^A[AT]TC
             1694  GAATC               
Tsp509I       413  AATT                 ^AATT
             1453  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AvaII       BanI        BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaJI       BsgI        BsiYI       BslI        
BsmFI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraIII      DsaI        
EaeI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
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