Restriction map for pF1KSDA0115
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MflI        MluI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        191  TCCGGA               T^CCGGA
AcsI          271  GAATTC               [GA]^AATT[CT]
AcyI          828  GGCGCC               G[GA]^CG[CT]C
AflIII       1148  ACGTGT               A^C[GA][CT]GT
AlwNI          20  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1256  AGCGCT               AGC^GCT
ApaI           80  GGGCCC               GGGCC^C
ApoI          271  GAATTC               [GA]^AATT[CT]
AspHI         217  GAGCTC               G[AT]GC[AT]^C
AvaI           71  CCCGGG               C^[CT]CG[GA]G
BalI          501  TGGCCA               TGG^CCA
BanI          828  GGCGCC               G^G[CT][GA]CC
BbeI          828  GGCGCC               GGCGC^C
BbsI         1333  GTCTTC               GAAGAC(2/6)
BpuAI        1333  GTCTTC               GAAGAC(2/6)
BsaHI         828  GGCGCC               G[GA]^CG[CT]C
BsaI          340  GAGACC               GGTCTC(1/5)
BseAI         191  TCCGGA               T^CCGGA
BsiHKAI       217  GAGCTC               G[AT]GC[AT]^C
BsoBI          71  CCCGGG               C^[CT]CG[GA]G
BspEI         191  TCCGGA               T^CCGGA
BsrDI         729  CATTGC               GCAATG(2/0)
BsrFI        1184  ACCGGC               [GA]^CCGG[CT]
BstAPI        425  GCAGTGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        403  GGTGACC              G^GT[ACGT]ACC
BstPI         403  GGTGACC              G^GT[ACGT]ACC
BstXI        1301  CCATGATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       1184  ACCGGC               [GA]^CCGG[CT]
DraI         1168  TTTAAA               TTT^AAA
DsaI           65  CCGCGG               C^C[GA][CT]GG
Eco47III     1256  AGCGCT               AGC^GCT
EcoO65I       403  GGTGACC              G^GT[ACGT]ACC
EcoRI         271  GAATTC               G^AATTC
EcoT14I       498  CCTTGG               C^C[AT][AT]GG
HgaI          761  GCGTC                GACGC(5/10)
Hin1I         828  GGCGCC               G[GA]^CG[CT]C
KasI          828  GGCGCC               G^GCGCC
KspI           65  CCGCGG               CCGC^GG
MluNI         501  TGGCCA               TGG^CCA
MroI          191  TCCGGA               T^CCGGA
MscI          501  TGGCCA               TGG^CCA
MseI         1169  TTAA                 T^TAA
NarI          828  GGCGCC               GG^CGCC
NspI          702  ACATGC               [GA]CATG^[CT]
PvuII        1060  CAGCTG               CAG^CTG
SacI          217  GAGCTC               GAGCT^C
SacII          65  CCGCGG               CCGC^GG
SfoI          828  GGCGCC               GGC^GCC
SmaI           71  CCCGGG               CCC^GGG
StyI          498  CCTTGG               C^C[AT][AT]GG
Tru9I        1169  TTAA                 T^TAA
Tsp509I       272  AATT                 ^AATT
XmaCI          71  CCCGGG               C^CCGGG
XmaI           71  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI            6  GTAC                 GT^AC
             1206  GTAC                
BciVI         696  GTATCC               GTATCC(6/5)
             1222  GTATCC              
BsaWI          11  TCCGGT               [AT]^CCGG[AT]
              191  TCCGGA              
BsgI           17  GTGCAG               GTGCAG(16/14)
              817  GTGCAG              
BsmFI         933  GTCCC                GGGAC(10/14)
             1036  GTCCC               
BspMI         536  ACCTGC               ACCTGC(4/8)
               19  GCAGGT              
BsrI          693  CCAGT                ACTGG(1/-1)
             1164  CCAGT               
BstNI         741  CCAGG                CC^[AT]GG
              746  CCAGG               
EcoNI          32  CCTTCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              537  CCTGCTGAAGG         
EcoRII        741  CCAGG                ^CC[AT]GG
              746  CCAGG               
HinfI         174  GACTC                G^A[ACGT]TC
              796  GACTC               
HphI          403  GGTGA                GGTGA(8/7)
              689  TCACC               
MaeIII        404  GTGAC                ^GT[ACGT]AC
              979  GTCAC               
MvaI          741  CCAGG                CC^[AT]GG
              746  CCAGG               
PleI          174  GACTC                GAGTC(4/5)
              796  GACTC               
RsaI            6  GTAC                 GT^AC
             1206  GTAC                
SapI          650  GCTCTTC              GCTCTTC(1/4)
             1314  GCTCTTC             
Tsp45I        404  GTGAC                ^GT[GC]AC
              979  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        Ksp632I     
MaeI        MaeII       MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        TspRI       TthHB8I     
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