Restriction map for pF1KSDA0115
 (Linear) (Six-base) MAPSORT of: KIAA0115.seq  Check: 9770  from: 1  to: 1368



KIAA0115.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      10    1368 
   Size:         10    1358
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     562     753     774    1123    1308    1368 
   Size:        220     342     191      21     349     185      60

  Fragments arranged by size: 

                 349     342     220     191     185      60      21
 
AfeI AGC'GCT

Cuts at:      0    1258    1368 
   Size:       1258     110
 
AflIII A'CryG_T

Cuts at:      0    1148    1368 
   Size:       1148     220
 
AleI CACnn'nnGTG

Cuts at:      0     142    1368 
   Size:        142    1226
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     160    1368 
   Size:        160    1208
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25    1368 
   Size:         25    1343
 
ApaI G_GGCC'C

Cuts at:      0      84    1368 
   Size:         84    1284
 
ApoI r'AATT_y

Cuts at:      0     271    1368 
   Size:        271    1097
 
AvaI C'yCGr_G

Cuts at:      0      71    1368 
   Size:         71    1297
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1205    1238    1368 
   Size:       1205      33     130

  Fragments arranged by size: 

                1205     130      33
 
BanI G'GyrC_C

Cuts at:      0     828    1368 
   Size:        828     540
 
BanII G_rGCy'C

Cuts at:      0      59      84     221     652     740    1316    1368 
   Size:         59      25     137     431      88     576      52

  Fragments arranged by size: 

                 576     431     137      88      59      52      25
 
BbeI G_GCGC'C

Cuts at:      0     832    1368 
   Size:        832     536
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1326    1368 
   Size:       1326      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     707    1233    1368 
   Size:        707     526     135

  Fragments arranged by size: 

                 707     526     135
 
Bme1580I G_kGCm'C

Cuts at:      0      84    1368 
   Size:         84    1284
 
BmgBI CAC'GTC

Cuts at:      0    1149    1368 
   Size:       1149     219
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     687    1368 
   Size:        687     681
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724    1084    1222    1368 
   Size:        724     360     138     146

  Fragments arranged by size: 

                 724     360     146     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     334    1368 
   Size:        334    1034
 
BsaHI Gr'CG_yC

Cuts at:      0     829    1368 
   Size:        829     539
 
BsaWI w'CCGG_w

Cuts at:      0      11     191    1368 
   Size:         11     180    1177

  Fragments arranged by size: 

                1177     180      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     359     389    1368 
   Size:        359      30     979

  Fragments arranged by size: 

                 979     359      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57     466     715     919    1368 
   Size:         57     409     249     204     449

  Fragments arranged by size: 

                 449     409     249     204      57
 
BseYI C'CCAG_C

Cuts at:      0      25      58     244     420     733     890    1308    1368 
   Size:         25      33     186     176     313     157     418      60

  Fragments arranged by size: 

                 418     313     186     176     157      60      33      25
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     838    1368 
   Size:         38     800     530

  Fragments arranged by size: 

                 800     530      38
 
BsiHKAI G_wGCw'C

Cuts at:      0     221    1368 
   Size:        221    1147
 
Bsp1286I G_dGCh'C

Cuts at:      0      59      84     221     652     740    1316    1368 
   Size:         59      25     137     431      88     576      52

  Fragments arranged by size: 

                 576     431     137      88      59      52      25
 
BspEI T'CCGG_A

Cuts at:      0     191    1368 
   Size:        191    1177
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10     545    1368 
   Size:         10     535     823

  Fragments arranged by size: 

                 823     535      10
 
BsrDI GCAATG_nn'

Cuts at:      0     728    1368 
   Size:        728     640
 
BsrFI r'CCGG_y

Cuts at:      0    1184    1368 
   Size:       1184     184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     431    1368 
   Size:        431     937
 
BstEII G'GTnAC_C

Cuts at:      0     403    1368 
   Size:        403     965
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1308    1368 
   Size:       1308      60
 
BtgI C'CryG_G

Cuts at:      0      65    1368 
   Size:         65    1303
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1064    1368 
   Size:       1064     304
 
BtsI GCAGTG_nn'

Cuts at:      0     378     432     821    1368 
   Size:        378      54     389     547

  Fragments arranged by size: 

                 547     389     378      54
 
DraI TTT'AAA

Cuts at:      0    1170    1368 
   Size:       1170     198
 
EaeI y'GGCC_r

Cuts at:      0     501     608    1231    1368 
   Size:        501     107     623     137

  Fragments arranged by size: 

                 623     501     137     107
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     657    1215    1321    1368 
   Size:        416     241     558     106      47

  Fragments arranged by size: 

                 558     416     241     106      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     380    1368 
   Size:        380     988
 
EcoICRI GAG'CTC

Cuts at:      0     219    1368 
   Size:        219    1149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     562     724     753     774    1084    1123    1222 
   Size:        220     342     162      29      21     310      39      99

Cuts at:   1222    1308    1368 
   Size:         86      60

  Fragments arranged by size: 

                 342     310     220     162      99      86      60      39
                  29      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      36     541    1368 
   Size:         36     505     827

  Fragments arranged by size: 

                 827     505      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29      81     546     925    1035    1094    1368 
   Size:         29      52     465     379     110      59     274

  Fragments arranged by size: 

                 465     379     274     110      59      52      29
 
EcoRI G'AATT_C

Cuts at:      0     271    1368 
   Size:        271    1097
 
HaeII r_GCGC'y

Cuts at:      0     832    1260    1295    1368 
   Size:        832     428      35      73

  Fragments arranged by size: 

                 832     428      73      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346    1368 
   Size:        314      32    1022

  Fragments arranged by size: 

                1022     314      32
 
KasI G'GCGC_C

Cuts at:      0     828    1368 
   Size:        828     540
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    1368 
   Size:        419     949
 
MscI TGG'CCA

Cuts at:      0     503    1368 
   Size:        503     865
 
MslI CAynn'nnrTG

Cuts at:      0     142     334     482    1368 
   Size:        142     192     148     886

  Fragments arranged by size: 

                 886     192     148     142
 
MspA1I CmG'CkG

Cuts at:      0      67     128     235     773     890    1062    1368 
   Size:         67      61     107     538     117     172     306

  Fragments arranged by size: 

                 538     306     172     117     107      67      61
 
NarI GG'CG_CC

Cuts at:      0     829    1368 
   Size:        829     539
 
NspI r_CATG'y

Cuts at:      0     706    1368 
   Size:        706     662
 
PfoI T'CCnGG_A

Cuts at:      0     674    1368 
   Size:        674     694
 
PpuMI rG'GwC_Cy

Cuts at:      0      29    1035    1094    1368 
   Size:         29    1006      59     274

  Fragments arranged by size: 

                1006     274      59      29
 
PspOMI G'GGCC_C

Cuts at:      0      80    1368 
   Size:         80    1288
 
PvuII CAG'CTG

Cuts at:      0    1062    1368 
   Size:       1062     306
 
SacI G_AGCT'C

Cuts at:      0     221    1368 
   Size:        221    1147
 
SacII CC_GC'GG

Cuts at:      0      68    1368 
   Size:         68    1300
 
SanDI GG'GwC_CC

Cuts at:      0    1035    1368 
   Size:       1035     333
 
SapI GCTCTTCn'nnn_

Cuts at:      0     657    1321    1368 
   Size:        657     664      47

  Fragments arranged by size: 

                 664     657      47
 
SfoI GGC'GCC

Cuts at:      0     830    1368 
   Size:        830     538
 
SmaI CCC'GGG

Cuts at:      0      73    1368 
   Size:         73    1295
 
SrfI GCCC'GGGC

Cuts at:      0      73    1368 
   Size:         73    1295
 
StyI C'CwwG_G

Cuts at:      0     498    1368 
   Size:        498     870
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     706     882     978    1368 
   Size:        706     176      96     390

  Fragments arranged by size: 

                 706     390     176      96
 
TatI w'GTAC_w

Cuts at:      0    1205    1368 
   Size:       1205     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     359     391     681     713    1368 
   Size:        359      32     290      32     655

  Fragments arranged by size: 

                 655     359     290      32      32
 
XmaI C'CCGG_G

Cuts at:      0      71    1368 
   Size:         71    1297
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BciVI
 Bme1580I    BmgBI     BmrI     BpmI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NspI     PfoI    PpuMI   PspOMI    PvuII     SacI    SacII    SanDI
     SapI     SfoI     SmaI     SrfI     StyI    TaqII     TatI     TstI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrGI   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII     DrdI     EagI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SalI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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