Restriction map for pF1KSDA0093
 (Linear) (Six-base) MAPSORT of: KIAA0093.seq  Check: 9988  from: 1  to: 2700



KIAA0093.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1221    2700 
   Size:       1221    1479
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1726    2455    2641    2700 
   Size:       1726     729     186      59

  Fragments arranged by size: 

                1726     729     186      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2264    2700 
   Size:       2264     436
 
AlwNI CAG_nnn'CTG

Cuts at:      0     538     691    2700 
   Size:        538     153    2009

  Fragments arranged by size: 

                2009     538     153
 
ApoI r'AATT_y

Cuts at:      0      48     927    1364    1624    2297    2700 
   Size:         48     879     437     260     673     403

  Fragments arranged by size: 

                 879     673     437     403     260      48
 
AvaI C'yCGr_G

Cuts at:      0    2492    2700 
   Size:       2492     208
 
AvrII C'CTAG_G

Cuts at:      0     306    2700 
   Size:        306    2394
 
BanI G'GyrC_C

Cuts at:      0    2496    2700 
   Size:       2496     204
 
BanII G_rGCy'C

Cuts at:      0      32     958    2592    2700 
   Size:         32     926    1634     108

  Fragments arranged by size: 

                1634     926     108      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     776    1667    2700 
   Size:        776     891    1033

  Fragments arranged by size: 

                1033     891     776
 
BbvCI CC'TCA_GC

Cuts at:      0     245     494    2700 
   Size:        245     249    2206

  Fragments arranged by size: 

                2206     249     245
 
Bme1580I G_kGCm'C

Cuts at:      0    1550    2700 
   Size:       1550    1150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     804    2347    2700 
   Size:        804    1543     353

  Fragments arranged by size: 

                1543     804     353
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     566    1412    1539    2382    2700 
   Size:         55     511     846     127     843     318

  Fragments arranged by size: 

                 846     843     511     318     127      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     245     494    2700 
   Size:        245     249    2206

  Fragments arranged by size: 

                2206     249     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1158    2700 
   Size:       1158    1542
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     357    1150    2700 
   Size:        357     793    1550

  Fragments arranged by size: 

                1550     793     357
 
BsaAI yAC'GTr

Cuts at:      0     129    2700 
   Size:        129    2571
 
BsaWI w'CCGG_w

Cuts at:      0    1670    2700 
   Size:       1670    1030
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563    1772    2700 
   Size:        563    1209     928

  Fragments arranged by size: 

                1209     928     563
 
BseYI C'CCAG_C

Cuts at:      0     512    1238    1277    2700 
   Size:        512     726      39    1423

  Fragments arranged by size: 

                1423     726     512      39
 
BsiHKAI G_wGCw'C

Cuts at:      0     958    2592    2700 
   Size:        958    1634     108

  Fragments arranged by size: 

                1634     958     108
 
BsmI GAATG_Cn'

Cuts at:      0     909    2700 
   Size:        909    1791
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     958    1550    2592    2700 
   Size:         32     926     592    1042     108

  Fragments arranged by size: 

                1042     926     592     108      32
 
BspHI T'CATG_A

Cuts at:      0    2013    2700 
   Size:       2013     687
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1221    2700 
   Size:       1221    1479
 
BsrFI r'CCGG_y

Cuts at:      0     254    2700 
   Size:        254    2446
 
BsrGI T'GTAC_A

Cuts at:      0    1140    2700 
   Size:       1140    1560
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     724    2700 
   Size:        724    1976
 
BstEII G'GTnAC_C

Cuts at:      0    1233    2700 
   Size:       1233    1467
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1058    2700 
   Size:       1058    1642
 
BstYI r'GATC_y

Cuts at:      0    1350    1506    2286    2700 
   Size:       1350     156     780     414

  Fragments arranged by size: 

                1350     780     414     156
 
Bsu36I CC'TnA_GG

Cuts at:      0     671    2092    2700 
   Size:        671    1421     608

  Fragments arranged by size: 

                1421     671     608
 
BtsI GCAGTG_nn'

Cuts at:      0    1550    2700 
   Size:       1550    1150
 
DraI TTT'AAA

Cuts at:      0    2256    2700 
   Size:       2256     444
 
DraIII CAC_nnn'GTG

Cuts at:      0     724    2700 
   Size:        724    1976
 
EarI CTCTTCn'nnn_

Cuts at:      0     587     969    1433    2700 
   Size:        587     382     464    1267

  Fragments arranged by size: 

                1267     587     464     382
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1960    2700 
   Size:       1960     740
 
EcoICRI GAG'CTC

Cuts at:      0     956    2590    2700 
   Size:        956    1634     110

  Fragments arranged by size: 

                1634     956     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     566    1412    1539    1726    2382    2455    2641 
   Size:         55     511     846     127     187     656      73     186

Cuts at:   2641    2700 
   Size:         59

  Fragments arranged by size: 

                 846     656     511     187     186     127      73      59
                  55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1554    2700 
   Size:       1554    1146
 
EcoO109I rG'GnC_Cy

Cuts at:      0     617    1192    1417    2700 
   Size:        617     575     225    1283

  Fragments arranged by size: 

                1283     617     575     225
 
EcoRV GAT'ATC

Cuts at:      0     606     749    2700 
   Size:        606     143    1951

  Fragments arranged by size: 

                1951     606     143
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1282    2700 
   Size:       1282    1418
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     189     221     333     365     945     977    1879    1911 
   Size:        189      32     112      32     580      32     902      32

Cuts at:   1911    2008    2040    2700 
   Size:         97      32     660

  Fragments arranged by size: 

                 902     660     580     189     112      97      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     771    2700 
   Size:        771    1929
 
MfeI C'AATT_G

Cuts at:      0    2666    2700 
   Size:       2666      34
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1496    1934    2700 
   Size:        904     592     438     766

  Fragments arranged by size: 

                 904     766     592     438
 
MslI CAynn'nnrTG

Cuts at:      0    1454    2135    2700 
   Size:       1454     681     565

  Fragments arranged by size: 

                1454     681     565
 
MspA1I CmG'CkG

Cuts at:      0    1167    2700 
   Size:       1167    1533
 
NspI r_CATG'y

Cuts at:      0    1294    2700 
   Size:       1294    1406
 
PasI CC'CwG_GG

Cuts at:      0    2679    2700 
   Size:       2679      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157    1343    1772    1870    2700 
   Size:        157    1186     429      98     830

  Fragments arranged by size: 

                1186     830     429     157      98
 
PfoI T'CCnGG_A

Cuts at:      0      32    1428    2700 
   Size:         32    1396    1272

  Fragments arranged by size: 

                1396    1272      32
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1637    1669    2700 
   Size:       1637      32    1031

  Fragments arranged by size: 

                1637    1031      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     617    2700 
   Size:        617    2083
 
PshAI GACnn'nnGTC

Cuts at:      0    2541    2700 
   Size:       2541     159
 
PsiI TTA'TAA

Cuts at:      0     805    2700 
   Size:        805    1895
 
PstI C_TGCA'G

Cuts at:      0    1191    2700 
   Size:       1191    1509
 
PvuII CAG'CTG

Cuts at:      0    1167    2700 
   Size:       1167    1533
 
SacI G_AGCT'C

Cuts at:      0     958    2592    2700 
   Size:        958    1634     108

  Fragments arranged by size: 

                1634     958     108
 
SexAI A'CCwGG_T

Cuts at:      0    1053    2700 
   Size:       1053    1647
 
SfcI C'TryA_G

Cuts at:      0    1187    1852    2391    2700 
   Size:       1187     665     539     309

  Fragments arranged by size: 

                1187     665     539     309
 
SmlI C'TyrA_G

Cuts at:      0    1173    2700 
   Size:       1173    1527
 
SphI G_CATG'C

Cuts at:      0    1294    2700 
   Size:       1294    1406
 
SspI AAT'ATT

Cuts at:      0     227    1828    2041    2700 
   Size:        227    1601     213     659

  Fragments arranged by size: 

                1601     659     227     213
 
StuI AGG'CCT

Cuts at:      0      89     841    1308    2700 
   Size:         89     752     467    1392

  Fragments arranged by size: 

                1392     752     467      89
 
StyI C'CwwG_G

Cuts at:      0     306     609    2700 
   Size:        306     303    2091

  Fragments arranged by size: 

                2091     306     303
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1672    2692    2700 
   Size:       1672    1020       8

  Fragments arranged by size: 

                1672    1020       8
 
TatI w'GTAC_w

Cuts at:      0    1140    2700 
   Size:       1140    1560
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1529    1561    2700 
   Size:       1529      32    1139

  Fragments arranged by size: 

                1529    1139      32
 
XbaI T'CTAG_A

Cuts at:      0     640    2700 
   Size:        640    2060
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2686    2700 
   Size:       2686      14
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApoI     AvaI    AvrII     BanI
    BanII     BbsI    BbvCI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaWI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtsI     DraI   DraIII     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI    Hin4I   HincII     MfeI     MmeI
     MslI   MspA1I     NspI     PasI    PflMI     PfoI     PpiI    PpuMI
    PshAI     PsiI     PstI    PvuII     SacI    SexAI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaXI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI   BssHII
    BssSI    BstBI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI    EcoRI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com