Restriction map for pF1KSDA0091
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BamHI       BbsI        BcgI        BfrI        BlnI        
BpuAI       BsaBI       BsiWI       Bsp1407I    BspDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HpaI        
KpnI        KspI        MamI        MluI        NaeI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          244  GTAGAC               GT^[AC][GT]AC
AccIII       2129  TCCGGA               T^CCGGA
AhdI         1473  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       533  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1473  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        2046  CACGTG               CAC^GTG
BglI         1593  GCCTGACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1467  AGATCT               A^GATCT
BlpI          660  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      660  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2046  CACGTG               [CT]AC^GT[GA]
BsaI         2750  GAGACC               GGTCTC(1/5)
BseAI        2129  TCCGGA               T^CCGGA
BsmBI        2818  GAGACG               CGTCTC(1/5)
BspEI        2129  TCCGGA               T^CCGGA
BspHI        1748  TCATGA               T^CATGA
BsrBI        2937  GAGCGG               CCGCTC(-3/-3)
BssSI          31  CTCGTG               CACGAG(-5/-1)
BstAPI       3114  GCAGCTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         660  GCTGAGC              GC^T[ACGT]AGC
Eam1105I     1473  GACTGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2868  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         136  GAATTC               G^AATTC
HindIII         4  AAGCTT               A^AGCTT
MroI         2129  TCCGGA               T^CCGGA
MspA1I       2501  CAGCTG               C[AC]G^C[GT]G
NruI         2378  TCGCGA               TCG^CGA
PmaCI        2046  CACGTG               CAC^GTG
PmlI         2046  CACGTG               CAC^GTG
PpuMI        2027  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          589  CTGCAG               CTGCA^G
PvuI         2896  CGATCG               CGAT^CG
PvuII        2501  CAGCTG               CAG^CTG
RcaI         1748  TCATGA               T^CATGA
ScaI         2102  AGTACT               AGT^ACT
SfcI          589  CTGCAG               C^T[GA][CT]AG
SmaI         1360  CCCGGG               CCC^GGG
SphI          522  GCATGC               GCATG^C
XmaCI        1360  CCCGGG               C^CCGGG
XmaI         1360  CCCGGG               C^CCGGG
XmnI          533  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          136  GAATTC               [GA]^AATT[CT]
              207  AAATTC              
ApaI         1223  GGGCCC               GGGCC^C
             2036  GGGCCC              
ApoI          136  GAATTC               [GA]^AATT[CT]
              207  AAATTC              
AspHI         601  GTGCTC               G[AT]GC[AT]^C
             1284  GAGCAC              
AspI         1168  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1776  GACAGAGTC           
AvaI         1360  CCCGGG               C^[CT]CG[GA]G
             2032  CTCGGG              
BalI         1685  TGGCCA               TGG^CCA
             2339  TGGCCA              
BbeI         2823  GGCGCC               GGCGC^C
             2941  GGCGCC              
BbvCI         915  CCTCAGC              CCTCAGC(-5/-2)
             2503  GCTGAGG             
BciVI         184  GGATAC               GTATCC(6/5)
             2244  GGATAC              
BsaWI        2129  TCCGGA               [AT]^CCGG[AT]
             2248  ACCGGA              
BsgI         1793  GTGCAG               GTGCAG(16/14)
             2370  GTGCAG              
BsiEI        1152  CGGTCG               CG[GA][CT]^CG
             2896  CGATCG              
BsiHKAI       601  GTGCTC               G[AT]GC[AT]^C
             1284  GAGCAC              
BsmI         1526  GAATGC               GAATGC(1/-1)
              537  GCATTC              
BsoBI        1360  CCCGGG               C^[CT]CG[GA]G
             2032  CTCGGG              
BstYI        1418  AGATCC               [GA]^GATC[CT]
             1467  AGATCT              
KasI         2823  GGCGCC               G^GCGCC
             2941  GGCGCC              
MfeI          249  CAATTG               C^AATTG
             2541  CAATTG              
MflI         1418  AGATCC               [GA]^GATC[CT]
             1467  AGATCT              
MluNI        1685  TGGCCA               TGG^CCA
             2339  TGGCCA              
MscI         1685  TGGCCA               TGG^CCA
             2339  TGGCCA              
MunI          249  CAATTG               C^AATTG
             2541  CAATTG              
NarI         2823  GGCGCC               GG^CGCC
             2941  GGCGCC              
NcoI          744  CCATGG               C^CATGG
             3029  CCATGG              
PflFI        1168  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1776  GACAGAGTC           
SapI          231  GAAGAGC              GCTCTTC(1/4)
              488  GAAGAGC             
SfoI         2823  GGCGCC               GGC^GCC
             2941  GGCGCC              
SmlI          406  CTCAAG               C^T[CT][GA]AG
             2909  CTCAAG              
Tth111I      1168  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1776  GACAGAGTC           
XhoII        1418  AGATCC               [GA]^GATC[CT]
             1467  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AvaII       BanI        
BanII       BbvI        BclI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspLU11I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com