Restriction map for pF1KSDA0085
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeII       MaeIII      MfeI        MflI        MluI        
MroI        MseI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          607  GTCTAC               GT^[AC][GT]AC
AcyI          141  GGCGTC               G[GA]^CG[CT]C
AfaI          270  GTAC                 GT^AC
AflIII        611  ACATGT               A^C[GA][CT]GT
AlwI          176  GGATC                GGATC(4/5)
AlwNI         418  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          171  CCTCAGG              CC^T[ACGT]AGG
ApaI          756  GGGCCC               GGGCC^C
AspHI         112  GAGCAC               G[AT]GC[AT]^C
BalI           71  TGGCCA               TGG^CCA
BbsI          741  GAAGAC               GAAGAC(2/6)
BfaI          386  CTAG                 C^TAG
BpuAI         741  GAAGAC               GAAGAC(2/6)
BsaHI         141  GGCGTC               G[GA]^CG[CT]C
BsiHKAI       112  GAGCAC               G[AT]GC[AT]^C
BsmFI         288  GTCCC                GGGAC(10/14)
BspHI          89  TCATGA               T^CATGA
BspLU11I      611  ACATGT               A^CATGT
BsrI          546  CCAGT                ACTGG(1/-1)
BstF5I        441  CATCC                GGATG(2/0)
BstXI          62  CCACCGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        171  CCTCAGG              CC^T[ACGT]AGG
DraII         755  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI          132  CCATGG               C^C[GA][CT]GG
EaeI           71  TGGCCA               [CT]^GGCC[GA]
Eco81I        171  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      755  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       132  CCATGG               C^C[AT][AT]GG
FokI          441  CATCC                GGATG(9/13)
Hin1I         141  GGCGTC               G[GA]^CG[CT]C
MaeI          386  CTAG                 C^TAG
MluNI          71  TGGCCA               TGG^CCA
MscI           71  TGGCCA               TGG^CCA
MspA1I        418  CAGCTG               C[AC]G^C[GT]G
NcoI          132  CCATGG               C^CATGG
NheI          385  GCTAGC               G^CTAGC
NspI          611  ACATGT               [GA]CATG^[CT]
PflMI         693  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         418  CAGCTG               CAG^CTG
RcaI           89  TCATGA               T^CATGA
RsaI          270  GTAC                 GT^AC
SapI          216  GCTCTTC              GCTCTTC(1/4)
SmlI          361  CTCAAG               C^T[CT][GA]AG
StyI          132  CCATGG               C^C[AT][AT]GG
Tsp509I       511  AATT                 ^AATT
Van91I        693  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaII           3  GGACC                G^G[AT]CC
              702  GGTCC               
BanII         333  GGGCTC               G[GA]GC[CT]^C
              756  GGGCCC              
BcnI          261  CCGGG                CC^[GC]GG
              374  CCCGG               
BpmI          192  CTCCAG               CTGGAG(16/14)
              691  CTCCAG              
BsaBI         558  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              561  GATGATCATC          
BsaJI          68  CCCTGG               C^C[ACGT][ACGT]GG
              132  CCATGG              
BseRI         390  CTCCTC               GAGGAG(10/8)
              667  GAGGAG              
BspMI          31  ACCTGC               ACCTGC(4/8)
              272  ACCTGC              
BstNI          69  CCTGG                CC^[AT]GG
              525  CCAGG               
EarI          217  CTCTTC               CTCTTC(1/4)
              672  GAAGAG              
EcoRII         69  CCTGG                ^CC[AT]GG
              525  CCAGG               
FauI            8  CCCGC                CCCGC(4/6)
              352  CCCGC               
HapII         261  CCGG                 C^CGG
              375  CCGG                
HpaII         261  CCGG                 C^CGG
              375  CCGG                
HphI          209  TCACC                GGTGA(8/7)
              323  TCACC               
Ksp632I       217  CTCTTC               CTCTTC(1/4)
              672  GAAGAG              
MamI          558  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              561  GATGATCATC          
MspI          261  CCGG                 C^CGG
              375  CCGG                
MvaI           69  CCTGG                CC^[AT]GG
              525  CCAGG               
NciI          261  CCGGG                CC^[GC]GG
              374  CCCGG               
PstI          415  CTGCAG               CTGCA^G
              453  CTGCAG              
TaqI          110  TCGA                 T^CGA
              739  TCGA                
TspRI          18  CACTG                CA[GC]TG[ACGT][ACGT]^
              588  CACTG               
TthHB8I       110  TCGA                 T^CGA
              739  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        BbvI        BclI        BmyI        
BsiYI       BslI        Bsp1286I    BssKI       Cac8I       
Cfr13I      DdeI        DpnII       FbaI        Fnu4HI      
HaeIII      HgaI        ItaI        MboI        MboII       
MnlI        MslI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TseI        
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