Restriction map for pF1KSDA0083
 (Linear) (Six-base) MAPSORT of: KIAA0083.seq  Check: 4731  from: 1  to: 3180



KIAA0083.orf

 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1531    3180 
   Size:       1531    1649
 
AatII G_ACGT'C

Cuts at:      0    2997    3180 
   Size:       2997     183
 
AccI GT'mk_AC

Cuts at:      0    2897    3180 
   Size:       2897     283
 
Acc65I G'GTAC_C

Cuts at:      0     137    3180 
   Size:        137    3043
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1318    3180 
   Size:       1318    1862
 
AflII C'TTAA_G

Cuts at:      0    2505    3180 
   Size:       2505     675
 
AflIII A'CryG_T

Cuts at:      0     748    1458    1751    2224    3180 
   Size:        748     710     293     473     956

  Fragments arranged by size: 

                 956     748     710     473     293
 
AgeI A'CCGG_T

Cuts at:      0     134    3180 
   Size:        134    3046
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     224     256    3180 
   Size:        224      32    2924

  Fragments arranged by size: 

                2924     224      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     988    1296    3180 
   Size:        988     308    1884

  Fragments arranged by size: 

                1884     988     308
 
ApaI G_GGCC'C

Cuts at:      0    2323    3180 
   Size:       2323     857
 
ApoI r'AATT_y

Cuts at:      0     915    1988    2147    2261    2611    3180 
   Size:        915    1073     159     114     350     569

  Fragments arranged by size: 

                1073     915     569     350     159     114
 
AseI AT'TA_AT

Cuts at:      0    3112    3180 
   Size:       3112      68
 
AvrII C'CTAG_G

Cuts at:      0     802    3180 
   Size:        802    2378
 
BanI G'GyrC_C

Cuts at:      0     137     379    1513    3180 
   Size:        137     242    1134    1667

  Fragments arranged by size: 

                1667    1134     242     137
 
BanII G_rGCy'C

Cuts at:      0     445    2323    2406    3180 
   Size:        445    1878      83     774

  Fragments arranged by size: 

                1878     774     445      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1135    3180 
   Size:       1135    2045
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     368    1842    3180 
   Size:        368    1474    1338

  Fragments arranged by size: 

                1474    1338     368
 
BfrBI ATG'CAT

Cuts at:      0     239     675    3180 
   Size:        239     436    2505

  Fragments arranged by size: 

                2505     436     239
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1890    3180 
   Size:       1890    1290
 
Bme1580I G_kGCm'C

Cuts at:      0    2323    3053    3180 
   Size:       2323     730     127

  Fragments arranged by size: 

                2323     730     127
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3169    3180 
   Size:       3169      11
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1369    2458    2557    3180 
   Size:         40    1329    1089      99     623

  Fragments arranged by size: 

                1329    1089     623      99      40
 
Bpu10I CC'TnA_GC

Cuts at:      0    2175    3180 
   Size:       2175    1005
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    3180 
   Size:        991    2189
 
BsaHI Gr'CG_yC

Cuts at:      0    2994    3180 
   Size:       2994     186
 
BsaWI w'CCGG_w

Cuts at:      0     134    3180 
   Size:        134    3046
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64    3180 
   Size:         64    3116
 
BseYI C'CCAG_C

Cuts at:      0    1289    3180 
   Size:       1289    1891
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291    2494    3180 
   Size:        291    2203     686

  Fragments arranged by size: 

                2203     686     291
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     445    1209    2406    3180 
   Size:        121     324     764    1197     774

  Fragments arranged by size: 

                1197     774     764     324     121
 
BsmI GAATG_Cn'

Cuts at:      0    1084    1862    3180 
   Size:       1084     778    1318

  Fragments arranged by size: 

                1318    1084     778
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     445    1209    2323    2406    3053    3180 
   Size:        121     324     764    1114      83     647     127

  Fragments arranged by size: 

                1114     764     647     324     127     121      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1531    3180 
   Size:       1531    1649
 
BsrDI GCAATG_nn'

Cuts at:      0    2742    3180 
   Size:       2742     438
 
BsrFI r'CCGG_y

Cuts at:      0      63     134    3180 
   Size:         63      71    3046

  Fragments arranged by size: 

                3046      71      63
 
BsrGI T'GTAC_A

Cuts at:      0     162     947    2460    3180 
   Size:        162     785    1513     720

  Fragments arranged by size: 

                1513     785     720     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1743    3180 
   Size:       1743    1437
 
Bsu36I CC'TnA_GG

Cuts at:      0     244    3180 
   Size:        244    2936
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1912    3180 
   Size:       1912    1268
 
BtsI GCAGTG_nn'

Cuts at:      0     203    2573    3180 
   Size:        203    2370     607

  Fragments arranged by size: 

                2370     607     203
 
DraI TTT'AAA

Cuts at:      0    2076    2589    3098    3180 
   Size:       2076     513     509      82

  Fragments arranged by size: 

                2076     513     509      82
 
EaeI y'GGCC_r

Cuts at:      0    2558    3180 
   Size:       2558     622
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     408     966    1160    1414    2186    3180 
   Size:         31     377     558     194     254     772     994

  Fragments arranged by size: 

                 994     772     558     377     254     194      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      70      82    3180 
   Size:         70      12    3098

  Fragments arranged by size: 

                3098      70      12
 
EcoICRI GAG'CTC

Cuts at:      0     443    2404    3180 
   Size:        443    1961     776

  Fragments arranged by size: 

                1961     776     443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1318    1369    2458    2557    3180 
   Size:         40    1278      51    1089      99     623

  Fragments arranged by size: 

                1278    1089     623      99      51      40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1952    3180 
   Size:       1952    1228
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2320    3180 
   Size:       2320     860
 
EcoRI G'AATT_C

Cuts at:      0    1988    3180 
   Size:       1988    1192
 
EcoRV GAT'ATC

Cuts at:      0     285    3180 
   Size:        285    2895
 
HaeII r_GCGC'y

Cuts at:      0     195    2707    3180 
   Size:        195    2512     473

  Fragments arranged by size: 

                2512     473     195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327     342     374     432     464    1342    1374 
   Size:        295      32      15      32      58      32     878      32

Cuts at:   1374    1962    1994    2275    2307    2530    2562    3112    3144 
   Size:        588      32     281      32     223      32     550      32

Cuts at:   3144    3180 
   Size:         36

  Fragments arranged by size: 

                 878     588     550     295     281     223      58      36
                  32      32      32      32      32      32      32      32
                  15
 
HincII GTy'rAC

Cuts at:      0    1343    2021    2046    2206    2468    3180 
   Size:       1343     678      25     160     262     712

  Fragments arranged by size: 

                1343     712     678     262     160      25
 
HindIII A'AGCT_T

Cuts at:      0    2421    3180 
   Size:       2421     759
 
HpaI GTT'AAC

Cuts at:      0    1343    2468    3180 
   Size:       1343    1125     712

  Fragments arranged by size: 

                1343    1125     712
 
KpnI G_GTAC'C

Cuts at:      0     141    3180 
   Size:        141    3039
 
MfeI C'AATT_G

Cuts at:      0    1200    1256    3180 
   Size:       1200      56    1924

  Fragments arranged by size: 

                1924    1200      56
 
MscI TGG'CCA

Cuts at:      0    2560    3180 
   Size:       2560     620
 
MslI CAynn'nnrTG

Cuts at:      0     124    3180 
   Size:        124    3056
 
MspA1I CmG'CkG

Cuts at:      0       9     888    3180 
   Size:          9     879    2292

  Fragments arranged by size: 

                2292     879       9
 
NaeI GCC'GGC

Cuts at:      0      65    3180 
   Size:         65    3115
 
NgoMIV G'CCGG_C

Cuts at:      0      63    3180 
   Size:         63    3117
 
NsiI A_TGCA'T

Cuts at:      0     241     677    3180 
   Size:        241     436    2503

  Fragments arranged by size: 

                2503     436     241
 
NspI r_CATG'y

Cuts at:      0     369     752    1462    2228    3180 
   Size:        369     383     710     766     952

  Fragments arranged by size: 

                 952     766     710     383     369
 
PciI A'CATG_T

Cuts at:      0     748    1458    2224    3180 
   Size:        748     710     766     956

  Fragments arranged by size: 

                 956     766     748     710
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     988    3159    3180 
   Size:        988    2171      21

  Fragments arranged by size: 

                2171     988      21
 
PspOMI G'GGCC_C

Cuts at:      0    2319    3180 
   Size:       2319     861
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1170    1202    3180 
   Size:       1170      32    1978

  Fragments arranged by size: 

                1978    1170      32
 
PvuII CAG'CTG

Cuts at:      0       9    3180 
   Size:          9    3171
 
SacI G_AGCT'C

Cuts at:      0     445    2406    3180 
   Size:        445    1961     774

  Fragments arranged by size: 

                1961     774     445
 
SapI GCTCTTCn'nnn_

Cuts at:      0     408     966    2186    3180 
   Size:        408     558    1220     994

  Fragments arranged by size: 

                1220     994     558     408
 
SexAI A'CCwGG_T

Cuts at:      0     140    3180 
   Size:        140    3040
 
SmlI C'TyrA_G

Cuts at:      0    1006    2505    3180 
   Size:       1006    1499     675

  Fragments arranged by size: 

                1499    1006     675
 
SspI AAT'ATT

Cuts at:      0    1183    1318    1486    2052    2249    3180 
   Size:       1183     135     168     566     197     931

  Fragments arranged by size: 

                1183     931     566     197     168     135
 
StyI C'CwwG_G

Cuts at:      0     802    2638    2907    3180 
   Size:        802    1836     269     273

  Fragments arranged by size: 

                1836     802     273     269
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1644    3151    3180 
   Size:       1644    1507      29

  Fragments arranged by size: 

                1644    1507      29
 
TatI w'GTAC_w

Cuts at:      0     162     947    1975    2460    2477    3180 
   Size:        162     785    1028     485      17     703

  Fragments arranged by size: 

                1028     785     703     485     162      17
 
XbaI T'CTAG_A

Cuts at:      0    1044    1591    2184    3180 
   Size:       1044     547     593     996

  Fragments arranged by size: 

                1044     996     593     547
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     469    1507    3180 
   Size:        469    1038    1673

  Fragments arranged by size: 

                1673    1038     469
 
XmnI GAAnn'nnTTC

Cuts at:      0    1731    3180 
   Size:       1731    1449
 
ZraI GAC'GTC

Cuts at:      0    2995    3180 
   Size:       2995     185
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AgeI
     AloI    AlwNI     ApaI     ApoI     AseI    AvrII     BanI    BanII
     BbsI    BciVI    BfrBI     BglI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BstXI   Bsu36I    BtgZI
     BtsI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI
     NspI     PciI    PflMI   PspOMI     PsrI    PvuII     SacI     SapI
    SexAI     SmlI     SspI     StyI    TaqII     TatI     XbaI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI     AhdI     AleI     AlfI    ApaLI     AscI    AsiSI
     AvaI     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BglII
     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaXI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI     ClaI    CspCI
   DraIII     DrdI     EagI     FalI     FseI     FspI    FspAI     KasI
     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XhoI     XmaI
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