Restriction map for pF1KSDA0081
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      Eam1105I    EarI        Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         436  GACGTC               GACGT^C
AfaI          567  GTAC                 GT^AC
AlwI          457  GGATC                GGATC(4/5)
AlwNI         263  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         247  GAGCAC               G[AT]GC[AT]^C
AvaI          112  CCCGGG               C^[CT]CG[GA]G
BmyI          247  GAGCAC               G[AGT]GC[ACT]^C
BsaI          255  GAGACC               GGTCTC(1/5)
BseRI         581  GAGGAG               GAGGAG(10/8)
BsiEI          98  CGGCCG               CG[GA][CT]^CG
BsiHKAI       247  GAGCAC               G[AT]GC[AT]^C
BsmFI         115  GGGAC                GGGAC(10/14)
BsoBI         112  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      247  GAGCAC               G[AGT]GC[ACT]^C
BspHI         341  TCATGA               T^CATGA
BspMI          53  ACCTGC               ACCTGC(4/8)
BsrDI         204  GCAATG               GCAATG(2/0)
BssHII         19  GCGCGC               G^CGCGC
DdeI          374  CTGAG                C^T[ACGT]AG
DraII          88  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          514  GACTTTTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           29  CCGTGG               C^C[GA][CT]GG
EaeI           98  CGGCCG               [CT]^GGCC[GA]
EagI           98  CGGCCG               C^GGCCG
EclXI          98  CGGCCG               C^GGCCG
Eco52I         98  CGGCCG               C^GGCCG
EcoNI         402  CCTCTGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       88  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       662  CCAAGG               C^C[AT][AT]GG
HincII        271  GTCGAC               GT[CT]^[GA]AC
HindII        271  GTCGAC               GT[CT]^[GA]AC
MaeII         437  ACGT                 A^CGT
NlaIV          88  GGGTCC               GG[ACGT]^[ACGT]CC
PflMI          79  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          88  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          341  TCATGA               T^CATGA
RsaI          567  GTAC                 GT^AC
SalI          271  GTCGAC               G^TCGAC
SfaNI         178  GATGC                GCATC(5/9)
SmaI          112  CCCGGG               CCC^GGG
StyI          662  CCAAGG               C^C[AT][AT]GG
Tsp45I        352  GTCAC                ^GT[GC]AC
Tsp509I       393  AATT                 ^AATT
TspRI         354  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I         79  CCACCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         112  CCCGGG               C^CCGGG
XmaI          112  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          129  GTCTAC               GT^[AC][GT]AC
              271  GTCGAC              
AcyI          117  GACGCC               G[GA]^CG[CT]C
              436  GACGTC              
AluI          493  AGCT                 AG^CT
              678  AGCT                
AvaII          33  GGTCC                G^G[AT]CC
               89  GGTCC               
BglII         237  AGATCT               A^GATCT
              642  AGATCT              
BpmI          199  CTGGAG               CTGGAG(16/14)
              553  CTGGAG              
BsaHI         117  GACGCC               G[GA]^CG[CT]C
              436  GACGTC              
BsmAI         255  GAGAC                GTCTC(1/5)
              382  GAGAC               
BstYI         237  AGATCT               [GA]^GATC[CT]
              642  AGATCT              
HgaI          117  GACGC                GACGC(5/10)
              192  GCGTC               
Hin1I         117  GACGCC               G[GA]^CG[CT]C
              436  GACGTC              
HinfI         127  GAGTC                G^A[ACGT]TC
              336  GACTC               
MaeIII        352  GTCAC                ^GT[ACGT]AC
              547  GTAAC               
MflI          237  AGATCT               [GA]^GATC[CT]
              642  AGATCT              
NruI          165  TCGCGA               TCG^CGA
              483  TCGCGA              
PleI          336  GACTC                GAGTC(4/5)
              127  GAGTC               
TaqI          272  TCGA                 T^CGA
              675  TCGA                
TthHB8I       272  TCGA                 T^CGA
              675  TCGA                
XhoII         237  AGATCT               [GA]^GATC[CT]
              642  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        BbsI        BbvI        BcnI        
BpuAI       BsaJI       BsiYI       BslI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DpnII       EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MboI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      Sau3AI      
Sau96I      ScrFI       TseI        
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