Restriction map for pF1KSDA0081
 (Linear) (Six-base) MAPSORT of: KIAA0081.seq  Check: 6059  from: 1  to: 702



KIAA0081.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AatII G_ACGT'C

Cuts at:      0     440     702 
   Size:        440     262
 
AccI GT'mk_AC

Cuts at:      0     130     272     702 
   Size:        130     142     430

  Fragments arranged by size: 

                 430     142     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     702 
   Size:        245     457
 
AlwNI CAG_nnn'CTG

Cuts at:      0     268     702 
   Size:        268     434
 
AvaI C'yCGr_G

Cuts at:      0     112     702 
   Size:        112     590
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187     340     650     701     702 
   Size:        187     153     310      51       1

  Fragments arranged by size: 

                 310     187     153      51       1
 
BglII A'GATC_T

Cuts at:      0     237     642     702 
   Size:        237     405      60

  Fragments arranged by size: 

                 405     237      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     574     702 
   Size:        220     354     128

  Fragments arranged by size: 

                 354     220     128
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     249     702 
   Size:        249     453
 
BsaHI Gr'CG_yC

Cuts at:      0     118     437     702 
   Size:        118     319     265

  Fragments arranged by size: 

                 319     265     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     123     153     702 
   Size:        123      30     549

  Fragments arranged by size: 

                 549     123      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     596     702 
   Size:        596     106
 
BseYI C'CCAG_C

Cuts at:      0     679     702 
   Size:        679      23
 
BsiEI CG_ry'CG

Cuts at:      0     101     702 
   Size:        101     601
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     702 
   Size:        251     451
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     702 
   Size:        251     451
 
BspHI T'CATG_A

Cuts at:      0     341     702 
   Size:        341     361
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62     702 
   Size:         62     640
 
BsrDI GCAATG_nn'

Cuts at:      0     211     702 
   Size:        211     491
 
BssHII G'CGCG_C

Cuts at:      0      19     702 
   Size:         19     683
 
BstYI r'GATC_y

Cuts at:      0     237     642     702 
   Size:        237     405      60

  Fragments arranged by size: 

                 405     237      60
 
BtgI C'CryG_G

Cuts at:      0      29     702 
   Size:         29     673
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     500     702 
   Size:        500     202
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     520     702 
   Size:        520     182
 
EaeI y'GGCC_r

Cuts at:      0      98     702 
   Size:         98     604
 
EagI C'GGCC_G

Cuts at:      0      98     702 
   Size:         98     604
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     245     574     702 
   Size:        220      25     329     128

  Fragments arranged by size: 

                 329     220     128      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     406     702 
   Size:        406     296
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     702 
   Size:         89     613
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     256     702 
   Size:        256     446
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     634     666     702 
   Size:        634      32      36

  Fragments arranged by size: 

                 634      36      32
 
HincII GTy'rAC

Cuts at:      0     273     702 
   Size:        273     429
 
NruI TCG'CGA

Cuts at:      0     167     485     702 
   Size:        167     318     217

  Fragments arranged by size: 

                 318     217     167
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      85     702 
   Size:         85     617
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     702 
   Size:         89     613
 
SalI G'TCGA_C

Cuts at:      0     271     702 
   Size:        271     431
 
SmaI CCC'GGG

Cuts at:      0     114     702 
   Size:        114     588
 
StyI C'CwwG_G

Cuts at:      0     662     702 
   Size:        662      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     514     644     702 
   Size:        514     130      58

  Fragments arranged by size: 

                 514     130      58
 
XmaI C'CCGG_G

Cuts at:      0     112     702 
   Size:        112     590
 
ZraI GAC'GTC

Cuts at:      0     438     702 
   Size:        438     264
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AlwNI     AvaI     BbsI    BglII     BpmI
     BsaI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI   BssHII    BstYI     BtgI    BtgZI     DrdI
     EaeI     EagI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII
     NruI    PflMI    PpuMI     SalI     SmaI     StyI    TaqII     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com