Restriction map for pF1KSDA0080
 (Linear) (Six-base) MAPSORT of: KIAA0080.seq  Check: 8984  from: 1  to: 1293



KIAA0080.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0    1007    1293 
   Size:       1007     286
 
AgeI A'CCGG_T

Cuts at:      0      42     970    1293 
   Size:         42     928     323

  Fragments arranged by size: 

                 928     323      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     717    1293 
   Size:        301     416     576

  Fragments arranged by size: 

                 576     416     301
 
ApaLI G'TGCA_C

Cuts at:      0    1056    1293 
   Size:       1056     237
 
AvaI C'yCGr_G

Cuts at:      0     758    1293 
   Size:        758     535
 
BamHI G'GATC_C

Cuts at:      0     475    1293 
   Size:        475     818
 
BanI G'GyrC_C

Cuts at:      0     786    1293 
   Size:        786     507
 
BanII G_rGCy'C

Cuts at:      0     403     884    1252    1293 
   Size:        403     481     368      41

  Fragments arranged by size: 

                 481     403     368      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     637     655    1293 
   Size:        637      18     638

  Fragments arranged by size: 

                 638     637      18
 
BbvCI CC'TCA_GC

Cuts at:      0     205     739    1293 
   Size:        205     534     554

  Fragments arranged by size: 

                 554     534     205
 
BlpI GC'TnA_GC

Cuts at:      0     310    1293 
   Size:        310     983
 
Bme1580I G_kGCm'C

Cuts at:      0    1060    1200    1293 
   Size:       1060     140      93

  Fragments arranged by size: 

                1060     140      93
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     898    1293 
   Size:        898     395
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     868    1251    1293 
   Size:        437     431     383      42

  Fragments arranged by size: 

                 437     431     383      42
 
Bpu10I CC'TnA_GC

Cuts at:      0     205     739    1293 
   Size:        205     534     554

  Fragments arranged by size: 

                 554     534     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     193     667     979    1293 
   Size:        193     474     312     314

  Fragments arranged by size: 

                 474     314     312     193
 
BsaBI GATnn'nnATC

Cuts at:      0     964    1293 
   Size:        964     329
 
BsaWI w'CCGG_w

Cuts at:      0      42     970    1293 
   Size:         42     928     323

  Fragments arranged by size: 

                 928     323      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     249     279     426     456    1293 
   Size:        249      30     147      30     837

  Fragments arranged by size: 

                 837     249     147      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     472    1293 
   Size:        472     821
 
BseYI C'CCAG_C

Cuts at:      0     808    1293 
   Size:        808     485
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     709    1293 
   Size:        709     584
 
BsiEI CG_ry'CG

Cuts at:      0    1155    1293 
   Size:       1155     138
 
BsiHKAI G_wGCw'C

Cuts at:      0     884    1060    1293 
   Size:        884     176     233

  Fragments arranged by size: 

                 884     233     176
 
Bsp1286I G_dGCh'C

Cuts at:      0     403     884    1060    1200    1252    1293 
   Size:        403     481     176     140      52      41

  Fragments arranged by size: 

                 481     403     176     140      52      41
 
BsrDI GCAATG_nn'

Cuts at:      0    1025    1293 
   Size:       1025     268
 
BsrFI r'CCGG_y

Cuts at:      0      42      52     970    1293 
   Size:         42      10     918     323

  Fragments arranged by size: 

                 918     323      42      10
 
BstEII G'GTnAC_C

Cuts at:      0      93    1293 
   Size:         93    1200
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     375    1293 
   Size:        375     918
 
BstYI r'GATC_y

Cuts at:      0     324     346     475     577    1293 
   Size:        324      22     129     102     716

  Fragments arranged by size: 

                 716     324     129     102      22
 
BtgI C'CryG_G

Cuts at:      0     543    1293 
   Size:        543     750
 
DraIII CAC_nnn'GTG

Cuts at:      0    1204    1293 
   Size:       1204      89
 
EaeI y'GGCC_r

Cuts at:      0      50    1265    1293 
   Size:         50    1215      28

  Fragments arranged by size: 

                1215      50      28
 
EcoICRI GAG'CTC

Cuts at:      0     882    1293 
   Size:        882     411
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     868    1251    1293 
   Size:        437     431     383      42

  Fragments arranged by size: 

                 437     431     383      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     334     714    1293 
   Size:         65     269     380     579

  Fragments arranged by size: 

                 579     380     269      65
 
EcoRV GAT'ATC

Cuts at:      0     966    1122    1293 
   Size:        966     156     171

  Fragments arranged by size: 

                 966     171     156
 
FseI GG_CCGG'CC

Cuts at:      0      56    1293 
   Size:         56    1237
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     739     771    1293 
   Size:        739      32     522

  Fragments arranged by size: 

                 739     522      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      45    1293 
   Size:         45    1248
 
MscI TGG'CCA

Cuts at:      0    1267    1293 
   Size:       1267      26
 
MslI CAynn'nnrTG

Cuts at:      0     229     373     595    1293 
   Size:        229     144     222     698

  Fragments arranged by size: 

                 698     229     222     144
 
MspA1I CmG'CkG

Cuts at:      0     708    1293 
   Size:        708     585
 
NaeI GCC'GGC

Cuts at:      0      54    1293 
   Size:         54    1239
 
NgoMIV G'CCGG_C

Cuts at:      0      52    1293 
   Size:         52    1241
 
NspI r_CATG'y

Cuts at:      0     174    1293 
   Size:        174    1119
 
PasI CC'CwG_GG

Cuts at:      0     329     421     573    1293 
   Size:        329      92     152     720

  Fragments arranged by size: 

                 720     329     152      92
 
PfoI T'CCnGG_A

Cuts at:      0     533    1293 
   Size:        533     760
 
PpuMI rG'GwC_Cy

Cuts at:      0     714    1293 
   Size:        714     579
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     139     171     994    1026    1293 
   Size:        139      32     823      32     267

  Fragments arranged by size: 

                 823     267     139      32      32
 
PstI C_TGCA'G

Cuts at:      0     713    1293 
   Size:        713     580
 
PvuI CG_AT'CG

Cuts at:      0    1155    1293 
   Size:       1155     138
 
PvuII CAG'CTG

Cuts at:      0     708    1293 
   Size:        708     585
 
SacI G_AGCT'C

Cuts at:      0     884    1293 
   Size:        884     409
 
SexAI A'CCwGG_T

Cuts at:      0     716    1293 
   Size:        716     577
 
SfcI C'TryA_G

Cuts at:      0     699     709    1293 
   Size:        699      10     584

  Fragments arranged by size: 

                 699     584      10
 
SgrAI Cr'CCGG_yG

Cuts at:      0      42    1293 
   Size:         42    1251
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     249     281    1293 
   Size:        249      32    1012

  Fragments arranged by size: 

                1012     249      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1170    1293 
   Size:       1170     123
 

 Enzymes that do cut:

     AccI     AgeI    AlwNI    ApaLI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BlpI Bme1580I     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI   BstEII    BstXI    BstYI     BtgI   DraIII
     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRV     FseI    Hin4I     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI
    PpuMI     PsrI     PstI     PvuI    PvuII     SacI    SexAI     SfcI
    SgrAI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaHI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI    EcoNI
    EcoRI     FalI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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