Restriction map for pF1KSDA0070
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BsaAI       BsaI        BsiWI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       BstUI       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    EcoNI       
EcoRI       EcoT22I     FauI        FseI        FspI        
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccIII        965  TCCGGA               T^CCGGA
AocI          196  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1451  AGCGCT               AGC^GCT
AseI          514  ATTAAT               AT^TAAT
AsnI          514  ATTAAT               AT^TAAT
AspHI         124  GAGCTC               G[AT]GC[AT]^C
BamHI         392  GGATCC               G^GATCC
BanI         1424  GGCACC               G^G[CT][GA]CC
BclI         1389  TGATCA               T^GATCA
BcnI          939  CCGGG                CC^[GC]GG
BfaI          790  CTAG                 C^TAG
BpmI          896  CTCCAG               CTGGAG(16/14)
BsaBI         810  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         965  TCCGGA               [AT]^CCGG[AT]
BseAI         965  TCCGGA               T^CCGGA
BseRI         443  GAGGAG               GAGGAG(10/8)
BsgI           10  GTGCAG               GTGCAG(16/14)
BsiHKAI       124  GAGCTC               G[AT]GC[AT]^C
BsmBI          72  GAGACG               CGTCTC(1/5)
BsmI         1297  GAATGC               GAATGC(1/-1)
BspEI         965  TCCGGA               T^CCGGA
BspHI        1469  TCATGA               T^CATGA
BsrDI        1487  GCAATG               GCAATG(2/0)
Bst1107I     1494  GTATAC               GTA^TAC
BstEII       1110  GGTCACC              G^GT[ACGT]ACC
BstPI        1110  GGTCACC              G^GT[ACGT]ACC
BstXI        1119  CCACCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         392  GGATCC               [GA]^GATC[CT]
BstZ17I      1494  GTATAC               GTA^TAC
Bsu36I        196  CCTGAGG              CC^T[ACGT]AGG
CfoI         1452  GCGC                 GCG^C
DraII         192  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          833  CCGTGG               C^C[GA][CT]GG
Eco47III     1451  AGCGCT               AGC^GCT
Eco81I        196  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      192  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1110  GGTCACC              G^GT[ACGT]ACC
EcoRV        1273  GATATC               GAT^ATC
FbaI         1389  TGATCA               T^GATCA
HaeII        1451  AGCGCT               [GA]GCGC^[CT]
HhaI         1452  GCGC                 GCG^C
HinP1I       1452  GCGC                 G^CGC
HincII       1153  GTTGAC               GT[CT]^[GA]AC
HindII       1153  GTTGAC               GT[CT]^[GA]AC
HindIII      1336  AAGCTT               A^AGCTT
MaeI          790  CTAG                 C^TAG
MamI          810  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          392  GGATCC               [GA]^GATC[CT]
MroI          965  TCCGGA               T^CCGGA
MslI         1120  CACCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          939  CCGGG                CC^[GC]GG
NdeI          631  CATATG               CA^TATG
PpuMI         192  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         514  ATTAAT               AT^TAAT
RcaI         1469  TCATGA               T^CATGA
SacI          124  GAGCTC               GAGCT^C
SapI         1192  GAAGAGC              GCTCTTC(1/4)
ScaI         1698  AGTACT               AGT^ACT
SfiI         1129  GGCCCAGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1198  CTTGAG               C^T[CT][GA]AG
XcmI         1751  CCACTGATACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         392  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI          301  GTAGAC               GT^[AC][GT]AC
             1494  GTATAC              
AcyI           74  GACGCC               G[GA]^CG[CT]C
              956  GACGCC              
AfaI         1601  GTAC                 GT^AC
             1699  GTAC                
Asp700I       502  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1696  GAAGTACTTC          
BalI          470  TGGCCA               TGG^CCA
              836  TGGCCA              
BanII         124  GAGCTC               G[GA]GC[CT]^C
             1407  GAGCCC              
BbsI          274  GAAGAC               GAAGAC(2/6)
             1726  GAAGAC              
BglI            7  GCCGTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1130  GCCCAGAGGGC         
BlpI          141  GCTAAGC              GC^T[ACGT]AGC
              585  GCTGAGC             
BmyI          124  GAGCTC               G[AGT]GC[ACT]^C
             1407  GAGCCC              
Bpu1102I      141  GCTAAGC              GC^T[ACGT]AGC
              585  GCTGAGC             
BpuAI         274  GAAGAC               GAAGAC(2/6)
             1726  GAAGAC              
BsaHI          74  GACGCC               G[GA]^CG[CT]C
              956  GACGCC              
Bsp1286I      124  GAGCTC               G[AGT]GC[ACT]^C
             1407  GAGCCC              
BsrBI         738  CCGCTC               CCGCTC(-3/-3)
             1428  CCGCTC              
BsrI         1761  ACTGG                ACTGG(1/-1)
              960  CCAGT               
CelII         141  GCTAAGC              GC^T[ACGT]AGC
              585  GCTGAGC             
EagI            5  CGGCCG               C^GGCCG
               17  CGGCCG              
EclXI           5  CGGCCG               C^GGCCG
               17  CGGCCG              
Eco52I          5  CGGCCG               C^GGCCG
               17  CGGCCG              
EcoT14I      1547  CCAAGG               C^C[AT][AT]GG
             1553  CCAAGG              
Hin1I          74  GACGCC               G[GA]^CG[CT]C
              956  GACGCC              
MluNI         470  TGGCCA               TGG^CCA
              836  TGGCCA              
MscI          470  TGGCCA               TGG^CCA
              836  TGGCCA              
MseI          376  TTAA                 T^TAA
              515  TTAA                
PleI         1678  GACTC                GAGTC(4/5)
             1658  GAGTC               
PstI          343  CTGCAG               CTGCA^G
             1559  CTGCAG              
PvuII         256  CAGCTG               CAG^CTG
             1631  CAGCTG              
RsaI         1601  GTAC                 GT^AC
             1699  GTAC                
SfcI          343  CTGCAG               C^T[GA][CT]AG
             1559  CTGCAG              
StyI         1547  CCAAGG               C^C[AT][AT]GG
             1553  CCAAGG              
Tru9I         376  TTAA                 T^TAA
              515  TTAA                
XmnI          502  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1696  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BbvI        BsaJI       BsiEI       BsiYI       
BslI        BsmAI       BspMI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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