Restriction map for pF1KSDA0070
 (Circular) (Six-base) MAPSORT of: pF1KSDA0070.seq  Check: 3658  from: 1  to: 4930



pF1KSDA0070  4930 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    476    1678     476 
   Size:       1202    3728
 
AccI GT'mk_AC

Cuts at:    428    1621    1916    1966     428 
   Size:       1193     295      50    3392

  Fragments arranged by size: 

                3392    1193     295      50
 
Acc65I G'GTAC_C

Cuts at:   1944    1944 
   Size:       4930
 
AclI AA'CG_TT

Cuts at:   4770    4770 
   Size:       4930
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     406    1394    2753    3185    3934    4202     214 
   Size:        192     988    1359     432     749     268     942

  Fragments arranged by size: 

                1359     988     942     749     432     268     192
 
AfeI AGC'GCT

Cuts at:   1579    1579 
   Size:       4930
 
AflIII A'CryG_T

Cuts at:   2280    3386    4363    2280 
   Size:       1106     977    2847

  Fragments arranged by size: 

                2847    1106     977
 
AgeI A'CCGG_T

Cuts at:   3318    3318 
   Size:       4930
 
AlwNI CAG_nnn'CTG

Cuts at:   2233    3802    2233 
   Size:       1569    3361
 
ApaLI G'TGCA_C

Cuts at:   3700    3700 
   Size:       4930
 
ApoI r'AATT_y

Cuts at:    628     853    1388    1932    4283    4539     628 
   Size:        225     535     544    2351     256    1019

  Fragments arranged by size: 

                2351    1019     544     535     256     225
 
AseI AT'TA_AT

Cuts at:     20     641      20 
   Size:        621    4309
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4930
 
AvaI C'yCGr_G

Cuts at:   1948    1948 
   Size:       4930
 
BamHI G'GATC_C

Cuts at:    518    1953     518 
   Size:       1435    3495
 
BanI G'GyrC_C

Cuts at:   1550    1944    2358    2606    2641    1550 
   Size:        394     414     248      35    3839

  Fragments arranged by size: 

                3839     414     394     248      35
 
BanII G_rGCy'C

Cuts at:    254    1537    1942    2972     254 
   Size:       1283     405    1030    2212

  Fragments arranged by size: 

                2212    1283    1030     405
 
BbeI G_GCGC'C

Cuts at:   2362    2610    2362 
   Size:        248    4682
 
BbsI GAAGACnn'nnnn_

Cuts at:    407    1859     407 
   Size:       1452    3478
 
BciVI GTATCCnnnnn_n'

Cuts at:   2826    3589    4930    2826 
   Size:        763    1341    2826

  Fragments arranged by size: 

                2826    1341     763
 
BclI T'GATC_A

Cuts at:   1515    2448    1515 
   Size:        933    3997
 
BglI GCCn_nnn'nGGC

Cuts at:    139    1262     139 
   Size:       1123    3807
 
BglII A'GATC_T

Cuts at:   2443    4482    2443 
   Size:       2039    2891
 
BlpI GC'TnA_GC

Cuts at:    268     712    2040     268 
   Size:        444    1328    3158

  Fragments arranged by size: 

                3158    1328     444
 
Bme1580I G_kGCm'C

Cuts at:   2553    2646    3704    2553 
   Size:         93    1058    3779

  Fragments arranged by size: 

                3779    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2140    2221    2556    4702    2140 
   Size:         81     335    2146    2368

  Fragments arranged by size: 

                2368    2146     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1037    3295    1037 
   Size:       2258    2672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1007    1007 
   Size:       4930
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1345    2104    2983    3477    3775    1345 
   Size:        759     879     494     298    2500

  Fragments arranged by size: 

                2500     879     759     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4634    4634 
   Size:       4930
 
BsaAI yAC'GTr

Cuts at:   1920    2281    1920 
   Size:        361    4569
 
BsaBI GATnn'nnATC

Cuts at:      6     940    2442    4481       6 
   Size:        934    1502    2039     455

  Fragments arranged by size: 

                2039    1502     934     455
 
BsaHI Gr'CG_yC

Cuts at:    201    1083    2359    2607    3311    4807     201 
   Size:        882    1276     248     704    1496     324

  Fragments arranged by size: 

                1496    1276     882     704     324     248
 
BsaWI w'CCGG_w

Cuts at:   1091    2117    2638    3318    3592    3739    4150    1091 
   Size:       1026     521     680     274     147     411    1871

  Fragments arranged by size: 

                1871    1026     680     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    563     593     563 
   Size:         30    4900
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    584     584 
   Size:       4930
 
BseYI C'CCAG_C

Cuts at:    906    1763    2353    3111    3690    4186     906 
   Size:        857     590     758     579     496    1650

  Fragments arranged by size: 

                1650     857     758     590     579     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    157     157 
   Size:       4930
 
BsiEI CG_ry'CG

Cuts at:    104     134     146    1065    2470    2516    3726     104 
   Size:         30      12     919    1405      46    1210    1308

  Fragments arranged by size: 

                1405    1308    1210     919      46      30      12
 
BsiHKAI G_wGCw'C

Cuts at:    254    1942    2720    2910    3704     254 
   Size:       1688     778     190     794    1480

  Fragments arranged by size: 

                1688    1480     794     778     190
 
BsmI GAATG_Cn'

Cuts at:   1429    1429 
   Size:       4930
 
BsmBI CGTCTCn'nnnn_

Cuts at:    192     192 
   Size:       4930
 
Bsp1286I G_dGCh'C

Cuts at:    254    1537    1942    2553    2646    2720    2910    2972    3704 
   Size:       1283     405     611      93      74     190      62     732

Cuts at:   3704     254 
   Size:       1480

  Fragments arranged by size: 

                1480    1283     732     611     405     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   1091    4150    1091 
   Size:       3059    1871
 
BspHI T'CATG_A

Cuts at:   1595    4106    1595 
   Size:       2511    2419
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    476     502    1678    1978    2192    2494    2875     476 
   Size:         26    1176     300     214     302     381    2531

  Fragments arranged by size: 

                2531    1176     381     302     300     214      26
 
BsrBI CCG'CTC

Cuts at:     50     866    1556    3220    3274    4760    4925      50 
   Size:        816     690    1664      54    1486     165      55

  Fragments arranged by size: 

                1664    1486     816     690     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1620    2840    1620 
   Size:       1220    3710
 
BsrFI r'CCGG_y

Cuts at:   2926    3107    3318    2926 
   Size:        181     211    4538

  Fragments arranged by size: 

                4538     211     181
 
BssSI C'ACGA_G

Cuts at:   3199    3559    3199 
   Size:        360    4570
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2305    2305 
   Size:       4930
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4930
 
BstEII G'GTnAC_C

Cuts at:   1236    1236 
   Size:       4930
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1252    1252 
   Size:       4930
 
BstYI r'GATC_y

Cuts at:      1     518    1953    2420    2443    2778    3024    4038    4124 
   Size:        517    1435     467      23     335     246    1014      86

Cuts at:   4124    4136    4482       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1435    1014     517     467     449     346     335     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1622    1622 
   Size:       4930
 
Bsu36I CC'TnA_GG

Cuts at:    323     323 
   Size:       4930
 
BtgI C'CryG_G

Cuts at:    125     959     125 
   Size:        834    4096
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2878    3059    3222    2878 
   Size:        181     163    4586

  Fragments arranged by size: 

                4586     181     163
 
BtsI GCAGTG_nn'

Cuts at:    278     736    2171     278 
   Size:        458    1435    3037

  Fragments arranged by size: 

                3037    1435     458
 
DraI TTT'AAA

Cuts at:   1927    1927 
   Size:       4930
 
DrdI GACnn_nn'nnGTC

Cuts at:   2634    3494    2634 
   Size:        860    4070
 
EaeI y'GGCC_r

Cuts at:    131     143     596     962    2513    2687    3078    3105    4452 
   Size:         12     453     366    1551     174     391      27    1347

Cuts at:   4452     131 
   Size:        609

  Fragments arranged by size: 

                1551    1347     609     453     391     366     174      27
                  12
 
EagI C'GGCC_G

Cuts at:    131     143    2513     131 
   Size:         12    2370    2548

  Fragments arranged by size: 

                2548    2370      12
 
EarI CTCTTCn'nnn_

Cuts at:    190     323    1313    2951    3161     190 
   Size:        133     990    1638     210    1959

  Fragments arranged by size: 

                1959    1638     990     210     133
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3136    3448    3594    4740    3136 
   Size:        312     146    1146    3326

  Fragments arranged by size: 

                3326    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:    252    1940     252 
   Size:       1688    3242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     406    1007    1394    2753    3185    3934    4202     214 
   Size:        192     601     387    1359     432     749     268     942

  Fragments arranged by size: 

                1359     942     749     601     432     387     268     192
            
 
EcoO109I rG'GnC_Cy

Cuts at:    319    2067     319 
   Size:       1748    3182
 
EcoRI G'AATT_C

Cuts at:   1932    1932 
   Size:       4930
 
EcoRV GAT'ATC

Cuts at:   1401    1401 
   Size:       4930
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    570    2947     570 
   Size:       2377    2553
 
FspI TGC'GCA

Cuts at:   2709    2709 
   Size:       4930
 
HaeII r_GCGC'y

Cuts at:   1581    2362    2610    3634    4613    1581 
   Size:        781     248    1024     979    1898

  Fragments arranged by size: 

                1898    1024     979     781     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    912     944    1104    1136    2770    2800    2802    2832     912 
   Size:         32     160      32    1634      30       2      30    3010

  Fragments arranged by size: 

                3010    1634     160      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1281    1967    1281 
   Size:        686    4244
 
HindIII A'AGCT_T

Cuts at:   1462    1462 
   Size:       4930
 
KasI G'GCGC_C

Cuts at:   2358    2606    2358 
   Size:        248    4682
 
KpnI G_GTAC'C

Cuts at:   1948    1948 
   Size:       4930
 
MluI A'CGCG_T

Cuts at:   4363    4363 
   Size:       4930
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1832    3601    3785    1832 
   Size:       1769     184    2977

  Fragments arranged by size: 

                2977    1769     184
 
MscI TGG'CCA

Cuts at:    598     964    2689     598 
   Size:        366    1725    2839

  Fragments arranged by size: 

                2839    1725     366
 
MslI CAynn'nnrTG

Cuts at:   1250    2917    3044    1250 
   Size:       1667     127    3136

  Fragments arranged by size: 

                3136    1667     127
 
MspA1I CmG'CkG

Cuts at:    384    1650    1759    2039    2353    2713    3728    3973     384 
   Size:       1266     109     280     314     360    1015     245    1341

  Fragments arranged by size: 

                1341    1266    1015     360     314     280     245     109
            
 
NaeI GCC'GGC

Cuts at:   3109    3109 
   Size:       4930
 
NarI GG'CG_CC

Cuts at:   2359    2607    2359 
   Size:        248    4682
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4930
 
NdeI CA'TA_TG

Cuts at:    758     758 
   Size:       4930
 
NgoMIV G'CCGG_C

Cuts at:   3107    3107 
   Size:       4930
 
NruI TCG'CGA

Cuts at:   3291    3291 
   Size:       4930
 
NspI r_CATG'y

Cuts at:   1981    2481    3390    1981 
   Size:        500     909    3521

  Fragments arranged by size: 

                3521     909     500
 
PasI CC'CwG_GG

Cuts at:    949    1335     949 
   Size:        386    4544
 
PciI A'CATG_T

Cuts at:   3386    3386 
   Size:       4930
 
PfoI T'CCnGG_A

Cuts at:   1478    1478 
   Size:       4930
 
PmeI GTTT'AAAC

Cuts at:   1927    1927 
   Size:       4930
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1690    1722    4069    4101    1690 
   Size:         32    2347      32    2519

  Fragments arranged by size: 

                2519    2347      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    319     319 
   Size:       4930
 
PsiI TTA'TAA

Cuts at:    614     614 
   Size:       4930
 
PstI C_TGCA'G

Cuts at:    473    1689    1975    2660     473 
   Size:       1216     286     685    2743

  Fragments arranged by size: 

                2743    1216     685     286
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4930
 
PvuII CAG'CTG

Cuts at:    384    1759    2353    2713     384 
   Size:       1375     594     360    2601

  Fragments arranged by size: 

                2601    1375     594     360
 
RsrII CG'GwC_CG

Cuts at:   3123    3123 
   Size:       4930
 
SacI G_AGCT'C

Cuts at:    254    1942     254 
   Size:       1688    3242
 
SalI G'TCGA_C

Cuts at:   1965    1965 
   Size:       4930
 
SapI GCTCTTCn'nnn_

Cuts at:   1313    2951    3161    1313 
   Size:       1638     210    3082

  Fragments arranged by size: 

                3082    1638     210
 
SbfI CC_TGCA'GG

Cuts at:   1975    1975 
   Size:       4930
 
ScaI AGT'ACT

Cuts at:   1826    4447    1826 
   Size:       2621    2309
 
SfcI C'TryA_G

Cuts at:     33     469    1685    1971    2656    3651    3842      33 
   Size:        436    1216     286     685     995     191    1121

  Fragments arranged by size: 

                1216    1121     995     685     436     286     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1262    1262 
   Size:       4930
 
SfoI GGC'GCC

Cuts at:   2360    2608    2360 
   Size:        248    4682
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4930
 
SmaI CCC'GGG

Cuts at:   1950    1950 
   Size:       4930
 
SmlI C'TyrA_G

Cuts at:   1324    2083    2998    3492    3754    1324 
   Size:        759     915     494     262    2500

  Fragments arranged by size: 

                2500     915     759     494     262
 
SnaBI TAC'GTA

Cuts at:   1920    1920 
   Size:       4930
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4930
 
SphI G_CATG'C

Cuts at:   1981    2481    1981 
   Size:        500    4430
 
StyI C'CwwG_G

Cuts at:    125    1673    1679    2062    2416     125 
   Size:       1548       6     383     354    2639

  Fragments arranged by size: 

                2639    1548     383     354       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1262    1535    2511    2644    3103    3314    1262 
   Size:        273     976     133     459     211    2878

  Fragments arranged by size: 

                2878     976     459     273     211     133
 
TatI w'GTAC_w

Cuts at:   1726    1824    4445    1726 
   Size:         98    2621    2211

  Fragments arranged by size: 

                2621    2211      98
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    404     436     563     595    4447    4479     404 
   Size:         32     127      32    3852      32     855

  Fragments arranged by size: 

                3852     855     127      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2725    2725 
   Size:       4930
 
XbaI T'CTAG_A

Cuts at:   1959    1959 
   Size:       4930
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1884    2423    1884 
   Size:        539    4391
 
XmaI C'CCGG_G

Cuts at:   1948    1948 
   Size:       4930
 
XmnI GAAnn'nnTTC

Cuts at:    632    1826     632 
   Size:       1194    3736
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NruI     NspI     PasI     PciI     PfoI     PmeI     PpiI
    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI    BsrGI   BssHII     ClaI    CspCI   DraIII    EcoNI     FseI
    FspAI     HpaI     MfeI     NheI     NotI     NsiI     PacI    PflMI
     PmlI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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