Restriction map for pF1KSDA0065
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# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraII       DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     FauI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

Alw44I       1045  GTGCAC               G^TGCAC
AlwNI          79  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1045  GTGCAC               G^TGCAC
AvaII          87  GGACC                G^G[AT]CC
BbsI         2303  GAAGAC               GAAGAC(2/6)
BclI          261  TGATCA               T^GATCA
BfaI           92  CTAG                 C^TAG
BpmI          124  CTGGAG               CTGGAG(16/14)
BpuAI        2303  GAAGAC               GAAGAC(2/6)
BsrI          247  CCAGT                ACTGG(1/-1)
Cfr13I         87  GGACC                G^G[ACGT]CC
DraI           40  TTTAAA               TTT^AAA
DraIII       2390  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          208  CCATGG               C^C[GA][CT]GG
EcoNI         681  CCTTGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         795  GATATC               GAT^ATC
EcoT14I       208  CCATGG               C^C[AT][AT]GG
EcoT22I      1496  ATGCAT               ATGCA^T
FbaI          261  TGATCA               T^GATCA
HincII       1692  GTCAAC               GT[CT]^[GA]AC
HindII       1692  GTCAAC               GT[CT]^[GA]AC
MaeI           92  CTAG                 C^TAG
MspA1I        257  CAGCTG               C[AC]G^C[GT]G
NcoI          208  CCATGG               C^CATGG
NsiI         1496  ATGCAT               ATGCA^T
PshAI          88  GACCCTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         257  CAGCTG               CAG^CTG
SapI          202  GAAGAGC              GCTCTTC(1/4)
Sau96I         87  GGACC                G^G[ACGT]CC
SfcI          132  CTACAG               C^T[GA][CT]AG
SspI          532  AATATT               AAT^ATT
StyI          208  CCATGG               C^C[AT][AT]GG
TaqI         1032  TCGA                 T^CGA
TthHB8I      1032  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AspHI         643  GAGCAC               G[AT]GC[AT]^C
             1045  GTGCAC              
BanII         101  GGGCTC               G[GA]GC[CT]^C
             2135  GGGCTC              
BsiHKAI       643  GAGCAC               G[AT]GC[AT]^C
             1045  GTGCAC              
BsmAI         610  GAGAC                GTCTC(1/5)
              145  GTCTC               
BsmI         1153  GAATGC               GAATGC(1/-1)
             2077  GAATGC              
Bsp1407I     1956  TGTACA               T^GTACA
             2292  TGTACA              
BspHI         603  TCATGA               T^CATGA
             1746  TCATGA              
BsrDI        1157  GCAATG               GCAATG(2/0)
             2081  GCAATG              
BsrGI        1956  TGTACA               T^GTACA
             2292  TGTACA              
MslI         1384  CACACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1747  CATGAATGTG          
NlaIV          87  GGACCC               GG[ACGT]^[ACGT]CC
              278  GGAGCC              
PleI           28  GAGTC                GAGTC(4/5)
              597  GAGTC               
RcaI          603  TCATGA               T^CATGA
             1746  TCATGA              
SmlI          557  CTCAAG               C^T[CT][GA]AG
             1411  CTTGAG              
SspBI        1956  TGTACA               T^GTACA
             2292  TGTACA              
TfiI          424  GATTC                G^A[AT]TC
              630  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
BbvI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       DdeI        DpnII       EarI        EcoRI       
EcoRII      Fnu4HI      FokI        HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIII      NspI        RsaI        Sau3AI      ScrFI       
SfaNI       Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmnI        
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