Restriction map for pF1KSDA0065
 (Linear) (Six-base) MAPSORT of: KIAA0065.seq  Check: 303  from: 1  to: 2430



KIAA0065.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     937    2085    2430 
   Size:        168     769    1148     345

  Fragments arranged by size: 

                1148     769     345     168
 
AleI CACnn'nnGTG

Cuts at:      0    1388    2430 
   Size:       1388    1042
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84    2430 
   Size:         84    2346
 
ApaLI G'TGCA_C

Cuts at:      0    1045    2430 
   Size:       1045    1385
 
ApoI r'AATT_y

Cuts at:      0     226     502     748     823    1463    1675    1883    2095 
   Size:        226     276     246      75     640     212     208     212

Cuts at:   2095    2346    2430 
   Size:        251      84

  Fragments arranged by size: 

                 640     276     251     246     226     212     212     208
                  84      75
 
BanII G_rGCy'C

Cuts at:      0     105    2139    2430 
   Size:        105    2034     291

  Fragments arranged by size: 

                2034     291     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2310    2430 
   Size:       2310     120
 
BclI T'GATC_A

Cuts at:      0     261    2430 
   Size:        261    2169
 
BfrBI ATG'CAT

Cuts at:      0    1498    2430 
   Size:       1498     932
 
Bme1580I G_kGCm'C

Cuts at:      0    1049    2430 
   Size:       1049    1381
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145    2430 
   Size:        145    2285
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    1432    2430 
   Size:        542     890     998

  Fragments arranged by size: 

                 998     890     542
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     291     622    2430 
   Size:         85     206     331    1808

  Fragments arranged by size: 

                1808     331     206      85
 
BsiHKAI G_wGCw'C

Cuts at:      0     647    1049    2430 
   Size:        647     402    1381

  Fragments arranged by size: 

                1381     647     402
 
BsmI GAATG_Cn'

Cuts at:      0    1159    2083    2430 
   Size:       1159     924     347

  Fragments arranged by size: 

                1159     924     347
 
Bsp1286I G_dGCh'C

Cuts at:      0     105     647    1049    2139    2430 
   Size:        105     542     402    1090     291

  Fragments arranged by size: 

                1090     542     402     291     105
 
BspHI T'CATG_A

Cuts at:      0     603    1746    2430 
   Size:        603    1143     684

  Fragments arranged by size: 

                1143     684     603
 
BsrDI GCAATG_nn'

Cuts at:      0    1164    2088    2430 
   Size:       1164     924     342

  Fragments arranged by size: 

                1164     924     342
 
BsrGI T'GTAC_A

Cuts at:      0    1956    2292    2430 
   Size:       1956     336     138

  Fragments arranged by size: 

                1956     336     138
 
BtgI C'CryG_G

Cuts at:      0     208    2430 
   Size:        208    2222
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1146    1181    2070    2105    2430 
   Size:       1146      35     889      35     325

  Fragments arranged by size: 

                1146     889     325      35      35
 
DraI TTT'AAA

Cuts at:      0      42    2430 
   Size:         42    2388
 
DraIII CAC_nnn'GTG

Cuts at:      0    2395    2430 
   Size:       2395      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     197     703     719    2430 
   Size:        197     506      16    1711

  Fragments arranged by size: 

                1711     506     197      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     168     937    2085    2430 
   Size:        145      23     769    1148     345

  Fragments arranged by size: 

                1148     769     345     145      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     685    2430 
   Size:        685    1745
 
EcoRI G'AATT_C

Cuts at:      0     226    1463    1883    2430 
   Size:        226    1237     420     547

  Fragments arranged by size: 

                1237     547     420     226
 
EcoRV GAT'ATC

Cuts at:      0     797    2430 
   Size:        797    1633
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51     246     278     760     792    2430 
   Size:         19      32     195      32     482      32    1638

  Fragments arranged by size: 

                1638     482     195      32      32      32      19
 
HincII GTy'rAC

Cuts at:      0    1694    2430 
   Size:       1694     736
 
HindIII A'AGCT_T

Cuts at:      0     240    1004    1088    1928    2430 
   Size:        240     764      84     840     502

  Fragments arranged by size: 

                 840     764     502     240      84
 
MslI CAynn'nnrTG

Cuts at:      0    1388    1751    2430 
   Size:       1388     363     679

  Fragments arranged by size: 

                1388     679     363
 
MspA1I CmG'CkG

Cuts at:      0     259    2430 
   Size:        259    2171
 
NcoI C'CATG_G

Cuts at:      0     208    2430 
   Size:        208    2222
 
NsiI A_TGCA'T

Cuts at:      0    1500    2430 
   Size:       1500     930
 
NspI r_CATG'y

Cuts at:      0    1502    1661    2258    2430 
   Size:       1502     159     597     172

  Fragments arranged by size: 

                1502     597     172     159
 
PshAI GACnn'nnGTC

Cuts at:      0      92    2430 
   Size:         92    2338
 
PsiI TTA'TAA

Cuts at:      0     743    2430 
   Size:        743    1687
 
PvuII CAG'CTG

Cuts at:      0     259    2430 
   Size:        259    2171
 
SapI GCTCTTCn'nnn_

Cuts at:      0     197    2430 
   Size:        197    2233
 
SfcI C'TryA_G

Cuts at:      0     132    2430 
   Size:        132    2298
 
SmlI C'TyrA_G

Cuts at:      0     557    1411    2430 
   Size:        557     854    1019

  Fragments arranged by size: 

                1019     854     557
 
SspI AAT'ATT

Cuts at:      0     534    2430 
   Size:        534    1896
 
StyI C'CwwG_G

Cuts at:      0     208    2430 
   Size:        208    2222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79    2430 
   Size:         79    2351
 
TatI w'GTAC_w

Cuts at:      0    1368    1620    1956    2124    2292    2430 
   Size:       1368     252     336     168     168     138

  Fragments arranged by size: 

                1368     336     252     168     168     138
 
XmnI GAAnn'nnTTC

Cuts at:      0     922    1006    1510    1594    2098    2266    2430 
   Size:        922      84     504      84     504     168     164

  Fragments arranged by size: 

                 922     504     504     168     164      84      84
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI    ApaLI     ApoI    BanII     BbsI     BclI
    BfrBI Bme1580I     BpmI    BpuEI    BseRI  BsiHKAI     BsmI Bsp1286I
    BspHI    BsrDI    BsrGI     BtgI    CspCI     DraI   DraIII     EarI
  Eco57MI    EcoNI    EcoRI    EcoRV    Hin4I   HincII  HindIII     MslI
   MspA1I     NcoI     NsiI     NspI    PshAI     PsiI    PvuII     SapI
     SfcI     SmlI     SspI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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