Restriction map for pF1KSDA0064
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbsI        BclI        BfrI        
BglII       BlnI        BpuAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         873  CGCG                 CG^CG
AspHI         886  GAGCTC               G[AT]GC[AT]^C
AspI          634  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1274  CCCGGG               C^[CT]CG[GA]G
BalI          912  TGGCCA               TGG^CCA
BanII         886  GAGCTC               G[GA]GC[CT]^C
BbrPI          76  CACGTG               CAC^GTG
BbvCI         208  GCTGAGG              CCTCAGC(-5/-2)
BcgI          249  GCAAGCTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         472  GGATAC               GTATCC(6/5)
BfaI         1094  CTAG                 C^TAG
BpmI          427  CTGGAG               CTGGAG(16/14)
BsiHKAI       886  GAGCTC               G[AT]GC[AT]^C
BsmBI        1212  GAGACG               CGTCTC(1/5)
BsmI          172  GCATTC               GAATGC(1/-1)
BsoBI        1274  CCCGGG               C^[CT]CG[GA]G
BstAPI        950  GCATGCGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         873  CGCG                 CG^CG
DraII         332  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          912  TGGCCA               [CT]^GGCC[GA]
EarI          606  GAAGAG               CTCTTC(1/4)
EcoO109I      332  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HincII        391  GTCAAC               GT[CT]^[GA]AC
HindII        391  GTCAAC               GT[CT]^[GA]AC
HindIII       187  AAGCTT               A^AGCTT
Ksp632I       606  GAAGAG               CTCTTC(1/4)
MaeI         1094  CTAG                 C^TAG
MluNI         912  TGGCCA               TGG^CCA
MscI          912  TGGCCA               TGG^CCA
MvnI          873  CGCG                 CG^CG
NaeI         1190  GCCGGC               GCC^GGC
NcoI         1133  CCATGG               C^CATGG
NgoMIV       1190  GCCGGC               G^CCGGC
PflFI         634  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI          76  CACGTG               CAC^GTG
PmlI           76  CACGTG               CAC^GTG
PpuMI         332  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          895  CTGCAG               CTGCA^G
SacI          886  GAGCTC               GAGCT^C
SgrAI        1189  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         1274  CCCGGG               CCC^GGG
SphI          950  GCATGC               GCATG^C
Tth111I       634  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1274  CCCGGG               C^CCGGG
XmaI         1274  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwNI         115  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              790  CAGACGCTG           
Asp700I       186  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              768  GAAGGAGTTC          
AvaII         333  GGTCC                G^G[AT]CC
             1068  GGACC               
BlpI          366  GCTCAGC              GC^T[ACGT]AGC
              888  GCTCAGC             
BmyI          311  GGGCAC               G[AGT]GC[ACT]^C
              886  GAGCTC              
Bpu1102I      366  GCTCAGC              GC^T[ACGT]AGC
              888  GCTCAGC             
BsaAI          58  TACGTG               [CT]AC^GT[GA]
               76  CACGTG              
BsaBI        1113  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1399  GATGAGGATC          
BseRI         972  CTCCTC               GAGGAG(10/8)
              222  GAGGAG              
BsgI           91  CTGCAC               GTGCAG(16/14)
              127  CTGCAC              
Bsp1286I      311  GGGCAC               G[AGT]GC[ACT]^C
              886  GAGCTC              
BspHI        1058  TCATGA               T^CATGA
             1109  TCATGA              
BspMI         665  ACCTGC               ACCTGC(4/8)
              330  GCAGGT              
BsrDI         269  CATTGC               GCAATG(2/0)
              983  CATTGC              
BssSI         130  CACGAG               CACGAG(-5/-1)
              999  CACGAG              
BstF5I        420  GGATG                GGATG(2/0)
              507  GGATG               
CelII         366  GCTCAGC              GC^T[ACGT]AGC
              888  GCTCAGC             
DsaI         1133  CCATGG               C^C[GA][CT]GG
             1368  CCACGG              
FokI          420  GGATG                GGATG(9/13)
              507  GGATG               
MamI         1113  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1399  GATGAGGATC          
MspA1I         33  CAGCGG               C[AC]G^C[GT]G
               42  CAGCGG              
NspI          245  ACATGC               [GA]CATG^[CT]
              950  GCATGC              
PflMI         114  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              268  CCATTGCTTGG         
RcaI         1058  TCATGA               T^CATGA
             1109  TCATGA              
SmlI         1255  CTTGAG               C^T[CT][GA]AG
             1296  CTCAAG              
TaqI          495  TCGA                 T^CGA
             1385  TCGA                
Tsp509I      1329  AATT                 ^AATT
             1375  AATT                
TthHB8I       495  TCGA                 T^CGA
             1385  TCGA                
Van91I        114  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              268  CCATTGCTTGG         
XmnI          186  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              768  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BglI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsrFI       BsrI        BssKI       
BstEII      BstNI       BstPI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      TspRI       XhoII       
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