Restriction map for pF1KSDA0057
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BclI        BcnI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HapII       Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StyI        SwaI        
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          560  AAATTC               [GA]^AATT[CT]
AhdI          353  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          838  GATCC                GGATC(4/5)
AocI          214  CCTAAGG              CC^T[ACGT]AGG
ApoI          560  AAATTC               [GA]^AATT[CT]
AspEI         353  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          220  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          841  CCCGAG               C^[CT]CG[GA]G
AvaII         219  GGACC                G^G[AT]CC
BalI          794  TGGCCA               TGG^CCA
BbsI          445  GAAGAC               GAAGAC(2/6)
BciVI         415  GGATAC               GTATCC(6/5)
BfaI          692  CTAG                 C^TAG
BpuAI         445  GAAGAC               GAAGAC(2/6)
BsaAI         397  TACGTG               [CT]AC^GT[GA]
BsaI          190  GAGACC               GGTCTC(1/5)
BsgI          514  CTGCAC               GTGCAG(16/14)
BsmFI         991  GTCCC                GGGAC(10/14)
BsoBI         841  CCCGAG               C^[CT]CG[GA]G
BsrBI          31  CCGCTC               CCGCTC(-3/-3)
BstAPI        872  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1044  CCATGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        214  CCTAAGG              CC^T[ACGT]AGG
CfoI          924  GCGC                 GCG^C
DraII         218  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI         1084  CCACGG               C^C[GA][CT]GG
EaeI          794  TGGCCA               [CT]^GGCC[GA]
Eam1105I      353  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        214  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      218  AGGACCT              [GA]G^G[ACGT]CC[CT]
HaeII         923  GGCGCT               [GA]GCGC^[CT]
HgaI           95  GCGTC                GACGC(5/10)
HhaI          924  GCGC                 GCG^C
HinP1I        924  GCGC                 G^CGC
MaeI          692  CTAG                 C^TAG
MaeII         398  ACGT                 A^CGT
MluNI         794  TGGCCA               TGG^CCA
MscI          794  TGGCCA               TGG^CCA
NlaIV        1075  GGAACC               GG[ACGT]^[ACGT]CC
PflFI         220  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         218  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          658  CTGCAG               CTGCA^G
SapI           33  GCTCTTC              GCTCTTC(1/4)
ScaI          662  AGTACT               AGT^ACT
SmlI         1099  CTCAAG               C^T[CT][GA]AG
SphI          264  GCATGC               GCATG^C
StuI          908  AGGCCT               AGG^CCT
TaqI          116  TCGA                 T^CGA
Tsp509I       561  AATT                 ^AATT
Tth111I       220  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       116  TCGA                 T^CGA
XcmI          430  CCAAGAAGCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I        196  GTGCAC               G^TGCAC
              460  GTGCAC              
AlwNI         735  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              873  CAGGCTCTG           
ApaLI         196  GTGCAC               G^TGCAC
              460  GTGCAC              
Asp700I       480  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              726  GAAACTGTTC          
BpmI          574  CTCCAG               CTGGAG(16/14)
              608  CTGGAG              
BsmAI         190  GAGAC                GTCTC(1/5)
             1104  GTCTC               
BstEII        759  GGTTACC              G^GT[ACGT]ACC
             1039  GGTTACC             
BstPI         759  GGTTACC              G^GT[ACGT]ACC
             1039  GGTTACC             
DpnII         645  GATC                 ^GATC
              838  GATC                
EcoO65I       759  GGTTACC              G^GT[ACGT]ACC
             1039  GGTTACC             
MboI          645  GATC                 ^GATC
              838  GATC                
MseI          421  TTAA                 T^TAA
              622  TTAA                
NdeII         645  GATC                 ^GATC
              838  GATC                
NspI          264  GCATGC               [GA]CATG^[CT]
              456  GCATGT              
PleI          695  GACTC                GAGTC(4/5)
              989  GAGTC               
Sau3AI        645  GATC                 ^GATC
              838  GATC                
SexAI         221  ACCTGGT              A^CC[AT]GGT
              593  ACCTGGT             
SfcI          176  CTACAG               C^T[GA][CT]AG
              658  CTGCAG              
TfiI          346  GAATC                G^A[AT]TC
             1033  GAATC               
Tru9I         421  TTAA                 T^TAA
              622  TTAA                
XmnI          480  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              726  GAAACTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BbvI        
BglI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeIII      
MboII       MnlI        MslI        MspA1I      MvaI        
MwoI        NlaIII      PvuII       RsaI        Sau96I      
ScrFI       TseI        Tsp45I      TspRI       
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