Restriction map for pF1KSDA0050
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspHI       
AviII       AvrII       BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BlnI        BpuAI       
BsaBI       BseAI       BsiEI       BsiHKAI     BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        494  GGTACC               G^GTACC
AflII         774  CTTAAG               C^TTAAG
AflIII        723  ACACGT               A^C[GA][CT]GT
Asp700I      1548  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       494  GGTACC               G^GTACC
AspI         2059  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BfrI          774  CTTAAG               C^TTAAG
BglI          445  GCCCAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1448  AGATCT               A^GATCT
BsaWI        1370  TCCGGT               [AT]^CCGG[AT]
BsmI         1319  GCATTC               GAATGC(1/-1)
BspHI        2202  TCATGA               T^CATGA
BsrFI         632  GCCGGC               [GA]^CCGG[CT]
BssSI         695  CACGAG               CACGAG(-5/-1)
BstXI        2033  CCATCGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        632  GCCGGC               [GA]^CCGG[CT]
DraIII       1216  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         687  GAATTC               G^AATTC
HincII       1792  GTCAAC               GT[CT]^[GA]AC
HindII       1792  GTCAAC               GT[CT]^[GA]AC
HindIII       771  AAGCTT               A^AGCTT
KpnI          494  GGTACC               GGTAC^C
NaeI          632  GCCGGC               GCC^GGC
NgoMIV        632  GCCGGC               G^CCGGC
PflFI        2059  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2202  TCATGA               T^CATGA
SmaI         1115  CCCGGG               CCC^GGG
SpeI         1404  ACTAGT               A^CTAGT
StuI         1520  AGGCCT               AGG^CCT
Tth111I      2059  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          291  TCTAGA               T^CTAGA
XmaCI        1115  CCCGGG               C^CCGGG
XmaI         1115  CCCGGG               C^CCGGG
XmnI         1548  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          244  AAATTC               [GA]^AATT[CT]
              687  GAATTC              
AfaI          495  GTAC                 GT^AC
              900  GTAC                
ApoI          244  AAATTC               [GA]^AATT[CT]
              687  GAATTC              
BbrPI         724  CACGTG               CAC^GTG
             1216  CACGTG              
BfaI          292  CTAG                 C^TAG
             1405  CTAG                
BlpI          627  GCTGAGC              GC^T[ACGT]AGC
             2187  GCTGAGC             
Bpu1102I      627  GCTGAGC              GC^T[ACGT]AGC
             2187  GCTGAGC             
BsmBI         937  CGTCTC               CGTCTC(1/5)
             2128  GAGACG              
BsrDI        1074  CATTGC               GCAATG(2/0)
             1247  GCAATG              
BstAPI        463  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1004  GCAAGTCCTGC         
CelII         627  GCTGAGC              GC^T[ACGT]AGC
             2187  GCTGAGC             
EcoNI         333  CCTGGTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1014  CCTCCTCCAGG         
MaeI          292  CTAG                 C^TAG
             1405  CTAG                
MseI          775  TTAA                 T^TAA
              839  TTAA                
MslI         1473  CATGGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2034  CATCGCCATG          
PaeR7I        485  CTCGAG               C^TCGAG
             2003  CTCGAG              
PmaCI         724  CACGTG               CAC^GTG
             1216  CACGTG              
PmlI          724  CACGTG               CAC^GTG
             1216  CACGTG              
PshAI         330  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              931  GACCTTCGTC          
RsaI          495  GTAC                 GT^AC
              900  GTAC                
TfiI         1527  GATTC                G^A[AT]TC
             1575  GATTC               
Tru9I         775  TTAA                 T^TAA
              839  TTAA                
XhoI          485  CTCGAG               C^TCGAG
             2003  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvI        BcnI        
BmyI        BpmI        BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        StyI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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