Restriction map for pF1KSDA0050
 (Linear) (Six-base) MAPSORT of: KIAA0050.seq  Check: 8783  from: 1  to: 2220



KIAA0050.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

Acc65I G'GTAC_C

Cuts at:      0     494    2220 
   Size:        494    1726
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1071    2033    2120    2220 
   Size:       1071     962      87     100

  Fragments arranged by size: 

                1071     962     100      87
 
AflII C'TTAA_G

Cuts at:      0     774    2220 
   Size:        774    1446
 
AflIII A'CryG_T

Cuts at:      0     723    2220 
   Size:        723    1497
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1537    1569    2220 
   Size:       1537      32     651

  Fragments arranged by size: 

                1537     651      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     333     469     857    2220 
   Size:        333     136     388    1363

  Fragments arranged by size: 

                1363     388     333     136
 
ApaI G_GGCC'C

Cuts at:      0     447    1496    1719    1927    2220 
   Size:        447    1049     223     208     293

  Fragments arranged by size: 

                1049     447     293     223     208
 
ApoI r'AATT_y

Cuts at:      0     244     687    2220 
   Size:        244     443    1533

  Fragments arranged by size: 

                1533     443     244
 
AvaI C'yCGr_G

Cuts at:      0     485    1115    2003    2220 
   Size:        485     630     888     217

  Fragments arranged by size: 

                 888     630     485     217
 
BanI G'GyrC_C

Cuts at:      0     146     437     494     661    1601    2220 
   Size:        146     291      57     167     940     619

  Fragments arranged by size: 

                 940     619     291     167     146      57
 
BanII G_rGCy'C

Cuts at:      0     221     447    1135    1174    1496    1682    1719    1927 
   Size:        221     226     688      39     322     186      37     208

Cuts at:   1927    1964    2004    2220 
   Size:         37      40     216

  Fragments arranged by size: 

                 688     322     226     221     216     208     186      40
                  39      37      37
 
BbeI G_GCGC'C

Cuts at:      0     150     441     665    1605    2220 
   Size:        150     291     224     940     615

  Fragments arranged by size: 

                 940     615     291     224     150
 
BglI GCCn_nnn'nGGC

Cuts at:      0     451    2220 
   Size:        451    1769
 
BglII A'GATC_T

Cuts at:      0    1448    2220 
   Size:       1448     772
 
BlpI GC'TnA_GC

Cuts at:      0     628    2188    2220 
   Size:        628    1560      32

  Fragments arranged by size: 

                1560     628      32
 
Bme1580I G_kGCm'C

Cuts at:      0     127     447     510    1217    1496    1628    1719    1927 
   Size:        127     320      63     707     279     132      91     208

Cuts at:   1927    2220 
   Size:        293

  Fragments arranged by size: 

                 707     320     293     279     208     132     127      91
                  63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130     636     660    1248    1806    2220 
   Size:        130     506      24     588     558     414

  Fragments arranged by size: 

                 588     558     506     414     130      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     500    1701    2220 
   Size:        500    1201     519

  Fragments arranged by size: 

                1201     519     500
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     867    1003    1864    2220 
   Size:        421     446     136     861     356

  Fragments arranged by size: 

                 861     446     421     356     136
 
Bpu10I CC'TnA_GC

Cuts at:      0    1134    2220 
   Size:       1134    1086
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16    1683    2220 
   Size:         16    1667     537

  Fragments arranged by size: 

                1667     537      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      88     136    1379    2220 
   Size:         88      48    1243     841

  Fragments arranged by size: 

                1243     841      88      48
 
BsaAI yAC'GTr

Cuts at:      0     726    1218    1542    2220 
   Size:        726     492     324     678

  Fragments arranged by size: 

                 726     678     492     324
 
BsaHI Gr'CG_yC

Cuts at:      0     147     438     662    1602    2167    2220 
   Size:        147     291     224     940     565      53

  Fragments arranged by size: 

                 940     565     291     224     147      53
 
BsaWI w'CCGG_w

Cuts at:      0    1370    2220 
   Size:       1370     850
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1335    1365    1537    1567    1775    1805    2220 
   Size:       1335      30     172      30     208      30     415

  Fragments arranged by size: 

                1335     415     208     172      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     637     772    1006    1867    2220 
   Size:         37     600     135     234     861     353

  Fragments arranged by size: 

                 861     600     353     234     135      37
 
BseYI C'CCAG_C

Cuts at:      0     490     657     747    1425    1495    2220 
   Size:        490     167      90     678      70     725

  Fragments arranged by size: 

                 725     678     490     167      90      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     927    1084    1690    1915    2220 
   Size:        927     157     606     225     305

  Fragments arranged by size: 

                 927     606     305     225     157
 
BsmI GAATG_Cn'

Cuts at:      0    1319    2220 
   Size:       1319     901
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     943    2122    2220 
   Size:        943    1179      98

  Fragments arranged by size: 

                1179     943      98
 
Bsp1286I G_dGCh'C

Cuts at:      0     127     221     447     510    1135    1174    1217    1496 
   Size:        127      94     226      63     625      39      43     279

Cuts at:   1496    1628    1682    1719    1927    1964    2004    2220 
   Size:        132      54      37     208      37      40     216

  Fragments arranged by size: 

                 625     279     226     216     208     132     127      94
                  63      54      43      40      39      37      37
 
BspHI T'CATG_A

Cuts at:      0    2202    2220 
   Size:       2202      18
 
BsrDI GCAATG_nn'

Cuts at:      0    1073    1254    2220 
   Size:       1073     181     966

  Fragments arranged by size: 

                1073     966     181
 
BsrFI r'CCGG_y

Cuts at:      0     632    2220 
   Size:        632    1588
 
BssSI C'ACGA_G

Cuts at:      0     695    2220 
   Size:        695    1525
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     469    1010    2220 
   Size:        469     541    1210

  Fragments arranged by size: 

                1210     541     469
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2040    2220 
   Size:       2040     180
 
BstYI r'GATC_y

Cuts at:      0     323    1448    1666    2220 
   Size:        323    1125     218     554

  Fragments arranged by size: 

                1125     554     323     218
 
BtgI C'CryG_G

Cuts at:      0    1472    1760    1779    2039    2220 
   Size:       1472     288      19     260     181

  Fragments arranged by size: 

                1472     288     260     181      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2019    2220 
   Size:       2019     201
 
BtsI GCAGTG_nn'

Cuts at:      0     310    1484    2220 
   Size:        310    1174     736

  Fragments arranged by size: 

                1174     736     310
 
DraIII CAC_nnn'GTG

Cuts at:      0    1221    2220 
   Size:       1221     999
 
EaeI y'GGCC_r

Cuts at:      0    1145    1184    1593    1651    1950    2220 
   Size:       1145      39     409      58     299     270

  Fragments arranged by size: 

                1145     409     299     270      58      39
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     697     820    1581    1757    1862    2220 
   Size:        452     245     123     761     176     105     358

  Fragments arranged by size: 

                 761     452     358     245     176     123     105
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     244    2220 
   Size:        244    1976
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     867    1003    1071    1864    2033    2120    2220 
   Size:        421     446     136      68     793     169      87     100

  Fragments arranged by size: 

                 793     446     421     169     136     100      87      68
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     337    1018    2220 
   Size:        337     681    1202

  Fragments arranged by size: 

                1202     681     337
 
EcoO109I rG'GnC_Cy

Cuts at:      0     784     906    1492    1646    1692    1715    1716    1923 
   Size:        784     122     586     154      46      23       1     207

Cuts at:   1923    2022    2220 
   Size:         99     198

  Fragments arranged by size: 

                 784     586     207     198     154     122      99      46
                  23       1
 
EcoRI G'AATT_C

Cuts at:      0     687    2220 
   Size:        687    1533
 
HaeII r_GCGC'y

Cuts at:      0     150     441     665    1038    1605    2220 
   Size:        150     291     224     373     567     615

  Fragments arranged by size: 

                 615     567     373     291     224     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     979    1011    1775    1807    2220 
   Size:        979      32     764      32     413

  Fragments arranged by size: 

                 979     764     413      32      32
 
HincII GTy'rAC

Cuts at:      0    1794    2220 
   Size:       1794     426
 
HindIII A'AGCT_T

Cuts at:      0     771    2220 
   Size:        771    1449
 
KasI G'GCGC_C

Cuts at:      0     146     437     661    1601    2220 
   Size:        146     291     224     940     619

  Fragments arranged by size: 

                 940     619     291     224     146
 
KpnI G_GTAC'C

Cuts at:      0     498    2220 
   Size:        498    1722
 
MscI TGG'CCA

Cuts at:      0    1147    1186    1952    2220 
   Size:       1147      39     766     268

  Fragments arranged by size: 

                1147     766     268      39
 
MslI CAynn'nnrTG

Cuts at:      0    1477    2038    2220 
   Size:       1477     561     182

  Fragments arranged by size: 

                1477     561     182
 
MspA1I CmG'CkG

Cuts at:      0     678    1047    1499    1830    1846    1929    2220 
   Size:        678     369     452     331      16      83     291

  Fragments arranged by size: 

                 678     452     369     331     291      83      16
 
NaeI GCC'GGC

Cuts at:      0     634    2220 
   Size:        634    1586
 
NarI GG'CG_CC

Cuts at:      0     147     438     662    1602    2220 
   Size:        147     291     224     940     618

  Fragments arranged by size: 

                 940     618     291     224     147
 
NcoI C'CATG_G

Cuts at:      0    1472    1760    1779    2039    2220 
   Size:       1472     288      19     260     181

  Fragments arranged by size: 

                1472     288     260     181      19
 
NgoMIV G'CCGG_C

Cuts at:      0     632    2220 
   Size:        632    1588
 
PasI CC'CwG_GG

Cuts at:      0    1167    1711    2109    2220 
   Size:       1167     544     398     111

  Fragments arranged by size: 

                1167     544     398     111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270     333    1239    2220 
   Size:        270      63     906     981

  Fragments arranged by size: 

                 981     906     270      63
 
PfoI T'CCnGG_A

Cuts at:      0     134    2220 
   Size:        134    2086
 
PmlI CAC'GTG

Cuts at:      0     726    1218    2220 
   Size:        726     492    1002

  Fragments arranged by size: 

                1002     726     492
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1537    1569    2220 
   Size:       1537      32     651

  Fragments arranged by size: 

                1537     651      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     906    1692    2022    2220 
   Size:        906     786     330     198

  Fragments arranged by size: 

                 906     786     330     198
 
PshAI GACnn'nnGTC

Cuts at:      0     334     935    2220 
   Size:        334     601    1285

  Fragments arranged by size: 

                1285     601     334
 
PspOMI G'GGCC_C

Cuts at:      0     443    1492    1715    1923    2220 
   Size:        443    1049     223     208     297

  Fragments arranged by size: 

                1049     443     297     223     208
 
PspXI vC'TCGA_Gb

Cuts at:      0     485    2220 
   Size:        485    1735
 
PstI C_TGCA'G

Cuts at:      0     317     527    1046    2220 
   Size:        317     210     519    1174

  Fragments arranged by size: 

                1174     519     317     210
 
PvuII CAG'CTG

Cuts at:      0     678    1047    1499    1846    2220 
   Size:        678     369     452     347     374

  Fragments arranged by size: 

                 678     452     374     369     347
 
SanDI GG'GwC_CC

Cuts at:      0    2022    2220 
   Size:       2022     198
 
SfcI C'TryA_G

Cuts at:      0     313     523    1042    2220 
   Size:        313     210     519    1178

  Fragments arranged by size: 

                1178     519     313     210
 
SfoI GGC'GCC

Cuts at:      0     148     439     663    1603    2220 
   Size:        148     291     224     940     617

  Fragments arranged by size: 

                 940     617     291     224     148
 
SmaI CCC'GGG

Cuts at:      0    1117    2220 
   Size:       1117    1103
 
SmlI C'TyrA_G

Cuts at:      0      31     485     774    1662    2003    2220 
   Size:         31     454     289     888     341     217

  Fragments arranged by size: 

                 888     454     341     289     217      31
 
SpeI A'CTAG_T

Cuts at:      0    1404    2220 
   Size:       1404     816
 
StuI AGG'CCT

Cuts at:      0    1522    2220 
   Size:       1522     698
 
StyI C'CwwG_G

Cuts at:      0    1302    1347    1472    1606    1760    1779    2039    2220 
   Size:       1302      45     125     134     154      19     260     181

  Fragments arranged by size: 

                1302     260     181     154     134     125      45      19
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     739    2220 
   Size:        316     423    1481

  Fragments arranged by size: 

                1481     423     316
 
TatI w'GTAC_w

Cuts at:      0     899    2220 
   Size:        899    1321
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64    1335    1367    1817    1849    2220 
   Size:         32      32    1271      32     450      32     371

  Fragments arranged by size: 

                1271     450     371      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2062    2220 
   Size:       2062     158
 
XbaI T'CTAG_A

Cuts at:      0     291    2220 
   Size:        291    1929
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1240    1283    1345    2220 
   Size:       1240      43      62     875

  Fragments arranged by size: 

                1240     875      62      43
 
XhoI C'TCGA_G

Cuts at:      0     485    2003    2220 
   Size:        485    1518     217

  Fragments arranged by size: 

                1518     485     217
 
XmaI C'CCGG_G

Cuts at:      0    1115    2220 
   Size:       1115    1105
 
XmnI GAAnn'nnTTC

Cuts at:      0    1552    2220 
   Size:       1552     668
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AloI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI     BsmI    BsmBI Bsp1286I
    BspHI    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII    SanDI     SfcI     SfoI     SmaI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI
    BsaBI    BsiEI  BsiHKAI    BsiWI    BspEI    BspMI    BsrBI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI
     DrdI     EagI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
     HpaI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PsiI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     SwaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com