Restriction map for pF1KSDA0035
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DpnII       DraI        DraIII      DsaI        Eam1105I    
Eco47III    EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MroI        MslI        MunI        
NarI        NcoI        NdeI        NdeII       NheI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        Sau3AI      
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I       1174  GGTACC               G^GTACC
AcsI         1185  GAATTC               [GA]^AATT[CT]
ApoI         1185  GAATTC               [GA]^AATT[CT]
Asp718I      1174  GGTACC               G^GTACC
AvaI          608  CTCGAG               C^[CT]CG[GA]G
BciVI          42  GTATCC               GTATCC(6/5)
BfaI          824  CTAG                 C^TAG
BsiEI           5  CGGCCG               CG[GA][CT]^CG
BsmI           14  GCATTC               GAATGC(1/-1)
BsoBI         608  CTCGAG               C^[CT]CG[GA]G
BsrFI          10  GCCGGC               [GA]^CCGG[CT]
CfoI           66  GCGC                 GCG^C
Cfr10I         10  GCCGGC               [GA]^CCGG[CT]
DrdI         1482  GACATCTAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
EcoRI        1185  GAATTC               G^AATTC
HhaI           66  GCGC                 GCG^C
HinP1I         66  GCGC                 G^CGC
KpnI         1174  GGTACC               GGTAC^C
MaeI          824  CTAG                 C^TAG
NaeI           10  GCCGGC               GCC^GGC
NgoMIV         10  GCCGGC               G^CCGGC
NotI            4  GCGGCCGC             GC^GGCCGC
PaeR7I        608  CTCGAG               C^TCGAG
SapI         1112  GCTCTTC              GCTCTTC(1/4)
SexAI         885  ACCAGGT              A^CC[AT]GGT
StuI          242  AGGCCT               AGG^CCT
TaqI          609  TCGA                 T^CGA
TthHB8I       609  TCGA                 T^CGA
XhoI          608  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII          22  CGCG                 CG^CG
               67  CGCG                
AocI         1417  CCTCAGG              CC^T[ACGT]AGG
             1822  CCTCAGG             
BanI         1174  GGTACC               G^G[CT][GA]CC
             1672  GGCACC              
BcnI          812  CCCGG                CC^[GC]GG
             2076  CCGGG               
BspMI         321  ACCTGC               ACCTGC(4/8)
             1221  ACCTGC              
BssSI        1940  CACGAG               CACGAG(-5/-1)
               49  CTCGTG              
BstAPI       1522  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1681  GCACTGACTGC         
BstUI          22  CGCG                 CG^CG
               67  CGCG                
Bsu36I       1417  CCTCAGG              CC^T[ACGT]AGG
             1822  CCTCAGG             
Eco81I       1417  CCTCAGG              CC^T[ACGT]AGG
             1822  CCTCAGG             
FauI          909  CCCGC                CCCGC(4/6)
             2064  GCGGG               
HindIII      1273  AAGCTT               A^AGCTT
             1840  AAGCTT              
MvnI           22  CGCG                 CG^CG
               67  CGCG                
NciI          812  CCCGG                CC^[GC]GG
             2076  CCGGG               
NlaIII        873  CATG                 CATG^
             2044  CATG                
PpuMI         185  AGGTCCC              [GA]G^G[AT]CC[CT]
              888  AGGTCCC             
SacI          495  GAGCTC               GAGCT^C
             1110  GAGCTC              
XcmI          956  CCAAGAAGGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1247  CCAAGCAACCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BglI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboII       MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MwoI        
NlaIV       PleI        PstI        PvuII       RsaI        
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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