Restriction map for pF1KSDA0035
 (Linear) (Six-base) MAPSORT of: KIAA0035.seq  Check: 91  from: 1  to: 2127



KIAA0035.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1230    2127 
   Size:       1230     897
 
Acc65I G'GTAC_C

Cuts at:      0    1174    2127 
   Size:       1174     953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    1100    1828    2127 
   Size:       1049      51     728     299

  Fragments arranged by size: 

                1049     728     299      51
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     936     968    2127 
   Size:        936      32    1159

  Fragments arranged by size: 

                1159     936      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     754    1132    1552    2127 
   Size:        754     378     420     575

  Fragments arranged by size: 

                 754     575     420     378
 
ApoI r'AATT_y

Cuts at:      0    1185    2127 
   Size:       1185     942
 
AvaI C'yCGr_G

Cuts at:      0     608    2127 
   Size:        608    1519
 
BanI G'GyrC_C

Cuts at:      0    1174    1672    2127 
   Size:       1174     498     455

  Fragments arranged by size: 

                1174     498     455
 
BanII G_rGCy'C

Cuts at:      0     499    1114    1642    2127 
   Size:        499     615     528     485

  Fragments arranged by size: 

                 615     528     499     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1004    1345    1486    2127 
   Size:       1004     341     141     641

  Fragments arranged by size: 

                1004     641     341     141
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      53    2127 
   Size:         53    2074
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1411    1663    1810    2127 
   Size:       1411     252     147     317

  Fragments arranged by size: 

                1411     317     252     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     781    1998    2127 
   Size:        781    1217     129

  Fragments arranged by size: 

                1217     781     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     855    1338    2127 
   Size:        855     483     789

  Fragments arranged by size: 

                 855     789     483
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     825    1073    1287    1308    1440    1587    2127 
   Size:        284     541     248     214      21     132     147     540

  Fragments arranged by size: 

                 541     540     284     248     214     147     132      21
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     501    1612    2127 
   Size:        157     344    1111     515

  Fragments arranged by size: 

                1111     515     344     157
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     505     535     936     966    1285    1315    2127 
   Size:        505      30     401      30     319      30     812

  Fragments arranged by size: 

                 812     505     401     319      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     292     295     427     467     706     709     871 
   Size:        289       3       3     132      40     239       3     162

Cuts at:    871    1345    1348    1406    1480    1483    1735    1738    1811 
   Size:        474       3      58      74       3     252       3      73

Cuts at:   1811    1933    2127 
   Size:        122     194

  Fragments arranged by size: 

                 474     289     252     239     194     162     132     122
                  74      73      58      40       3       3       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      31     947    2127 
   Size:         31     916    1180

  Fragments arranged by size: 

                1180     916      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1550    1664    1997    2127 
   Size:       1550     114     333     130

  Fragments arranged by size: 

                1550     333     130     114
 
BsiEI CG_ry'CG

Cuts at:      0       8    2127 
   Size:          8    2119
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     499    1114    2127 
   Size:         56     443     615    1013

  Fragments arranged by size: 

                1013     615     443      56
 
BsmI GAATG_Cn'

Cuts at:      0      14    2127 
   Size:         14    2113
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     499    1114    1642    2127 
   Size:         56     443     615     528     485

  Fragments arranged by size: 

                 615     528     485     443      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     330    1230    2127 
   Size:        330     900     897

  Fragments arranged by size: 

                 900     897     330
 
BsrFI r'CCGG_y

Cuts at:      0      10    2127 
   Size:         10    2117
 
BssSI C'ACGA_G

Cuts at:      0      49    1940    2127 
   Size:         49    1891     187

  Fragments arranged by size: 

                1891     187      49
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1528    1687    2127 
   Size:       1528     159     440

  Fragments arranged by size: 

                1528     440     159
 
Bsu36I CC'TnA_GG

Cuts at:      0    1418    1823    2127 
   Size:       1418     405     304

  Fragments arranged by size: 

                1418     405     304
 
BtsI GCAGTG_nn'

Cuts at:      0     390     684     834     999    1011    1232    1467    1757 
   Size:        390     294     150     165      12     221     235     290

Cuts at:   1757    2127 
   Size:        370

  Fragments arranged by size: 

                 390     370     294     290     235     221     165     150
                  12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1488    2127 
   Size:       1488     639
 
EaeI y'GGCC_r

Cuts at:      0       5      89     776    1266    1355    2127 
   Size:          5      84     687     490      89     772

  Fragments arranged by size: 

                 772     687     490      89      84       5
 
EagI C'GGCC_G

Cuts at:      0       5    2127 
   Size:          5    2122
 
EarI CTCTTCn'nnn_

Cuts at:      0     147     383     811     848    1028    1119    1307    1469 
   Size:        147     236     428      37     180      91     188     162

Cuts at:   1469    1607    1613    2127 
   Size:        138       6     514

  Fragments arranged by size: 

                 514     428     236     188     180     162     147     138
                  91      37       6
 
EcoICRI GAG'CTC

Cuts at:      0     497    1112    2127 
   Size:        497     615    1015

  Fragments arranged by size: 

                1015     615     497
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     825    1049    1073    1100    1287    1308    1440 
   Size:        284     541     224      24      27     187      21     132

Cuts at:   1440    1587    1828    2127 
   Size:        147     241     299

  Fragments arranged by size: 

                 541     299     284     241     224     187     147     132
                  27      24      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     186     295     783     889    2127 
   Size:        186     109     488     106    1238

  Fragments arranged by size: 

                1238     488     186     109     106
 
EcoRI G'AATT_C

Cuts at:      0    1185    2127 
   Size:       1185     942
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     499     505     531     537    1285    1317    1423    1455 
   Size:        499       6      26       6     748      32     106      32

Cuts at:   1455    1468    1500    2072    2104    2127 
   Size:         13      32     572      32      23

  Fragments arranged by size: 

                 748     572     499     106      32      32      32      32
                  26      23      13       6       6
 
HindIII A'AGCT_T

Cuts at:      0    1273    1840    2127 
   Size:       1273     567     287

  Fragments arranged by size: 

                1273     567     287
 
KpnI G_GTAC'C

Cuts at:      0    1178    2127 
   Size:       1178     949
 
MscI TGG'CCA

Cuts at:      0      91     778    1268    1357    2127 
   Size:         91     687     490      89     770

  Fragments arranged by size: 

                 770     687     490      91      89
 
MspA1I CmG'CkG

Cuts at:      0    1171    1204    1237    2127 
   Size:       1171      33      33     890

  Fragments arranged by size: 

                1171     890      33      33
 
NaeI GCC'GGC

Cuts at:      0      12    2127 
   Size:         12    2115
 
NgoMIV G'CCGG_C

Cuts at:      0      10    2127 
   Size:         10    2117
 
NotI GC'GGCC_GC

Cuts at:      0       5    2127 
   Size:          5    2122
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     936     968    2127 
   Size:        936      32    1159

  Fragments arranged by size: 

                1159     936      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     186     889    2127 
   Size:        186     703    1238

  Fragments arranged by size: 

                1238     703     186
 
PspXI vC'TCGA_Gb

Cuts at:      0     608    2127 
   Size:        608    1519
 
PstI C_TGCA'G

Cuts at:      0     360    1227    1242    1497    2127 
   Size:        360     867      15     255     630

  Fragments arranged by size: 

                 867     630     360     255      15
 
PvuII CAG'CTG

Cuts at:      0    1171    1204    1237    2127 
   Size:       1171      33      33     890

  Fragments arranged by size: 

                1171     890      33      33
 
SacI G_AGCT'C

Cuts at:      0     499    1114    2127 
   Size:        499     615    1013

  Fragments arranged by size: 

                1013     615     499
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1119    2127 
   Size:       1119    1008
 
SexAI A'CCwGG_T

Cuts at:      0     885    2127 
   Size:        885    1242
 
SfcI C'TryA_G

Cuts at:      0     159     356     894    1223    1238    1493    2127 
   Size:        159     197     538     329      15     255     634

  Fragments arranged by size: 

                 634     538     329     255     197     159      15
 
SmlI C'TyrA_G

Cuts at:      0     172     516     608    1627    2127 
   Size:        172     344      92    1019     500

  Fragments arranged by size: 

                1019     500     344     172      92
 
StuI AGG'CCT

Cuts at:      0     244    2127 
   Size:        244    1883
 
StyI C'CwwG_G

Cuts at:      0     182     461     779    1370    1661    1805    2127 
   Size:        182     279     318     591     291     144     322

  Fragments arranged by size: 

                 591     322     318     291     279     182     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     325    2127 
   Size:        325    1802
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     963    1254    2127 
   Size:        963     291     873

  Fragments arranged by size: 

                 963     873     291
 
XhoI C'TCGA_G

Cuts at:      0     608    2127 
   Size:        608    1519
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AloI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI    BciVI     BglI     BmrI     BplI     BpmI    BpuEI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrFI    BssSI   BstAPI   Bsu36I     BtsI     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I  HindIII
     KpnI     MscI   MspA1I     NaeI   NgoMIV     NotI     PpiI    PpuMI
    PspXI     PstI    PvuII     SacI     SapI    SexAI     SfcI     SmlI
     StuI     StyI    TaqII     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EciI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com