Restriction map for pF1KSDA0032
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BanI        BbeI        
BbvCI       BcgI        BclI        BfrI        BlnI        
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HgaI        Hin1I       HindIII     
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    RsrII       
SacII       SalI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

BamHI         566  GGATCC               G^GATCC
BbrPI        2337  CACGTG               CAC^GTG
BglI          589  GCCGGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1097  AGATCT               A^GATCT
BsaAI        2337  CACGTG               [CT]AC^GT[GA]
BsiEI        2961  CGATCG               CG[GA][CT]^CG
BsmI         2159  GCATTC               GAATGC(1/-1)
BspLU11I      169  ACATGT               A^CATGT
BsrDI         442  CATTGC               GCAATG(2/0)
BssSI        1858  CTCGTG               CACGAG(-5/-1)
BstAPI       2218  GCAGTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          800  GCGC                 GCG^C
DraIII        133  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           97  GACAGAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          495  CCATGG               C^C[GA][CT]GG
EcoNI        1542  CCTACTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       950  ATGCAT               ATGCA^T
FauI         3020  GCGGG                CCCGC(4/6)
HaeII         799  GGCGCT               [GA]GCGC^[CT]
HhaI          800  GCGC                 GCG^C
HinP1I        800  GCGC                 G^CGC
HpaI          847  GTTAAC               GTT^AAC
NcoI          495  CCATGG               C^CATGG
NsiI          950  ATGCAT               ATGCA^T
PflMI         282  CCACAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2337  CACGTG               CAC^GTG
PmlI         2337  CACGTG               CAC^GTG
PpuMI         553  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuI         2961  CGATCG               CGAT^CG
PvuII         310  CAGCTG               CAG^CTG
SapI         2769  GCTCTTC              GCTCTTC(1/4)
SbfI           51  CCTGCAGG             CCTGCA^GG
SexAI          33  ACCTGGT              A^CC[AT]GGT
SmaI         2533  CCCGGG               CCC^GGG
SmlI         2764  CTTGAG               C^T[CT][GA]AG
Sse8387I       51  CCTGCAGG             CCTGCA^GG
Van91I        282  CCACAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        2533  CCCGGG               C^CCGGG
XmaI         2533  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI          457  GCGG                 CCGC(-3/-1)
             3020  GCGG                
AcsI         2245  GAATTT               [GA]^AATT[CT]
             2662  GAATTT              
AlwI          567  GATCC                GGATC(4/5)
              566  GGATC               
AlwNI         623  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2003  CAGATGCTG           
ApoI         2245  GAATTT               [GA]^AATT[CT]
             2662  GAATTT              
AvaI         1061  CCCGAG               C^[CT]CG[GA]G
             2533  CCCGGG              
AvaII         554  GGTCC                G^G[AT]CC
             2106  GGTCC               
BciVI        1608  GGATAC               GTATCC(6/5)
             1935  GGATAC              
BlpI           67  GCTGAGC              GC^T[ACGT]AGC
             2154  GCTGAGC             
Bpu1102I       67  GCTGAGC              GC^T[ACGT]AGC
             2154  GCTGAGC             
BsgI          326  GTGCAG               GTGCAG(16/14)
             1694  GTGCAG              
BsoBI        1061  CCCGAG               C^[CT]CG[GA]G
             2533  CCCGGG              
BsrFI        2227  GCCGGT               [GA]^CCGG[CT]
             3036  GCCGGT              
BstXI          75  CCAGGTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1853  CCAATCTCGTGG        
BstYI         566  GGATCC               [GA]^GATC[CT]
             1097  AGATCT              
CelII          67  GCTGAGC              GC^T[ACGT]AGC
             2154  GCTGAGC             
Cfr10I       2227  GCCGGT               [GA]^CCGG[CT]
             3036  GCCGGT              
DraI         1075  TTTAAA               TTT^AAA
             1345  TTTAAA              
DraII         553  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             3116  AGGCCCT             
EcoO109I      553  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             3116  AGGCCCT             
HincII        847  GTTAAC               GT[CT]^[GA]AC
             1162  GTTGAC              
HindII        847  GTTAAC               GT[CT]^[GA]AC
             1162  GTTGAC              
MaeII        2114  ACGT                 A^CGT
             2338  ACGT                
MflI          566  GGATCC               [GA]^GATC[CT]
             1097  AGATCT              
MslI          258  CATTCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1249  CACAACAATG          
MspA1I        310  CAGCTG               C[AC]G^C[GT]G
             3018  CAGCGG              
SacI          236  GAGCTC               GAGCT^C
             2767  GAGCTC              
SspI          414  AATATT               AAT^ATT
             1661  AATATT              
TaqI          687  TCGA                 T^CGA
             2414  TCGA                
Tsp45I        299  GTGAC                ^GT[GC]AC
             2490  GTCAC               
TthHB8I       687  TCGA                 T^CGA
             2414  TCGA                
XbaI          864  TCTAGA               T^CTAGA
             1434  TCTAGA              
XhoII         566  GGATCC               [GA]^GATC[CT]
             1097  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AfaI        AflIII      AluI        Asp700I     AspHI       
BalI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaBI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MamI        
MboI        MboII       MluNI       MnlI        MscI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XcmI        XmnI        
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