Restriction map for pF1KSDA0028
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AviII       AvrII       
BamHI       BanII       BbeI        BbsI        BcgI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       BstBI       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoT22I     FauI        FseI        FspI        
Hin1I       KasI        KpnI        KspI        MamI        
MfeI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AocI         2506  CCTGAGG              CC^T[ACGT]AGG
AspI         2590  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         931  CACGTG               CAC^GTG
BciVI        1560  GGATAC               GTATCC(6/5)
BglI          892  GCCTATACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2393  AGATCT               A^GATCT
BsgI          289  CTGCAC               GTGCAG(16/14)
BsmBI        1310  GAGACG               CGTCTC(1/5)
Bsp1407I     1667  TGTACA               T^GTACA
BsrGI        1667  TGTACA               T^GTACA
Bst1107I      150  GTATAC               GTA^TAC
BstEII       2214  GGTGACC              G^GT[ACGT]ACC
BstPI        2214  GGTGACC              G^GT[ACGT]ACC
BstZ17I       150  GTATAC               GTA^TAC
Bsu36I       2506  CCTGAGG              CC^T[ACGT]AGG
CfoI         2410  GCGC                 GCG^C
Eco81I       2506  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      2214  GGTGACC              G^GT[ACGT]ACC
EcoRV        2023  GATATC               GAT^ATC
HaeII        2409  GGCGCT               [GA]GCGC^[CT]
HhaI         2410  GCGC                 GCG^C
HinP1I       2410  GCGC                 G^CGC
HincII        613  GTTAAC               GT[CT]^[GA]AC
HindII        613  GTTAAC               GT[CT]^[GA]AC
HindIII      2543  AAGCTT               A^AGCTT
HpaI          613  GTTAAC               GTT^AAC
MluI          761  ACGCGT               A^CGCGT
PflFI        2590  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         931  CACGTG               CAC^GTG
PmlI          931  CACGTG               CAC^GTG
PstI         2497  CTGCAG               CTGCA^G
ScaI         1132  AGTACT               AGT^ACT
SmlI          990  CTTGAG               C^T[CT][GA]AG
SphI          800  GCATGC               GCATG^C
SspBI        1667  TGTACA               T^GTACA
SspI          457  AATATT               AAT^ATT
Tth111I      2590  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          150  GTATAC               GT^[AC][GT]AC
              310  GTCTAC              
AccII         762  CGCG                 CG^CG
             1452  CGCG                
AflIII        526  ACGTGT               A^C[GA][CT]GT
              761  ACGCGT              
AlwNI         224  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2143  CAGCTGCTG           
Asp700I      2602  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2658  GAAGTCCTTC          
BanI          922  GGCACC               G^G[CT][GA]CC
             2415  GGTGCC              
BbvCI        2140  CCTCAGC              CCTCAGC(-5/-2)
             2167  GCTGAGG             
BclI         1737  TGATCA               T^GATCA
             2531  TGATCA              
BfaI         1136  CTAG                 C^TAG
             1270  CTAG                
BspMI         360  GCAGGT               ACCTGC(4/8)
              654  GCAGGT              
BsrDI         155  CATTGC               GCAATG(2/0)
             2279  GCAATG              
BstAPI       1013  GCAAAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2470  GCATGGCCTGC         
BstUI         762  CGCG                 CG^CG
             1452  CGCG                
BstXI         436  CCACAAAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              810  CCACTGGATTGG        
BstYI        1851  GGATCT               [GA]^GATC[CT]
             2393  AGATCT              
EarI           38  CTCTTC               CTCTTC(1/4)
             1296  GAAGAG              
EcoNI        1514  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1791  CCTTATCAAGG         
EcoRI        1124  GAATTC               G^AATTC
             2247  GAATTC              
FbaI         1737  TGATCA               T^GATCA
             2531  TGATCA              
Ksp632I        38  CTCTTC               CTCTTC(1/4)
             1296  GAAGAG              
MaeI         1136  CTAG                 C^TAG
             1270  CTAG                
MflI         1851  GGATCT               [GA]^GATC[CT]
             2393  AGATCT              
MvnI          762  CGCG                 CG^CG
             1452  CGCG                
NcoI          374  CCATGG               C^CATGG
             1487  CCATGG              
PpuMI         620  GGGACCC              [GA]G^G[AT]CC[CT]
             1064  AGGTCCC             
SmaI         1289  CCCGGG               CCC^GGG
             2579  CCCGGG              
XcmI          647  CCAATGAGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1085  CCAAAGCTGACTTGG     
XhoII        1851  GGATCT               [GA]^GATC[CT]
             2393  AGATCT              
XmaCI        1289  CCCGGG               C^CCGGG
             2579  CCCGGG              
XmaI         1289  CCCGGG               C^CCGGG
             2579  CCCGGG              
XmnI         2602  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2658  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BalI        BbvI        
BcnI        BmyI        BpmI        BsaAI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      
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