Restriction map for pF1KSDA0026
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmnI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AhdI          126  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          113  CCTCAGG              CC^T[ACGT]AGG
ApaI          305  GGGCCC               GGGCC^C
AspEI         126  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         671  GAGCAC               G[AT]GC[AT]^C
AvaI          308  CCCGGG               C^[CT]CG[GA]G
AvaII         133  GGTCC                G^G[AT]CC
BamHI         208  GGATCC               G^GATCC
BanII         305  GGGCCC               G[GA]GC[CT]^C
BbvCI         283  CCTCAGC              CCTCAGC(-5/-2)
BfaI           74  CTAG                 C^TAG
BpmI          475  CTGGAG               CTGGAG(16/14)
BsiHKAI       671  GAGCAC               G[AT]GC[AT]^C
BsoBI         308  CCCGGG               C^[CT]CG[GA]G
BsrDI         709  GCAATG               GCAATG(2/0)
BssSI          29  CTCGTG               CACGAG(-5/-1)
BstYI         208  GGATCC               [GA]^GATC[CT]
Bsu36I        113  CCTCAGG              CC^T[ACGT]AGG
DpnII         209  GATC                 ^GATC
DsaI          391  CCATGG               C^C[GA][CT]GG
Eam1105I      126  GACAGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           52  GAAGAG               CTCTTC(1/4)
Eco81I        113  CCTCAGG              CC^T[ACGT]AGG
EcoRI         795  GAATTC               G^AATTC
EcoT14I       391  CCATGG               C^C[AT][AT]GG
HapII         309  CCGG                 C^CGG
HpaII         309  CCGG                 C^CGG
HphI          639  TCACC                GGTGA(8/7)
Ksp632I        52  GAAGAG               CTCTTC(1/4)
MaeI           74  CTAG                 C^TAG
MaeIII        418  GTTAC                ^GT[ACGT]AC
MboI          209  GATC                 ^GATC
MflI          208  GGATCC               [GA]^GATC[CT]
MslI          640  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          309  CCGG                 C^CGG
NcoI          391  CCATGG               C^CATGG
NdeII         209  GATC                 ^GATC
NspI           83  ACATGC               [GA]CATG^[CT]
PleI          243  GACTC                GAGTC(4/5)
Sau3AI        209  GATC                 ^GATC
SmaI          308  CCCGGG               CCC^GGG
SspI          563  AATATT               AAT^ATT
StyI          391  CCATGG               C^C[AT][AT]GG
TfiI          372  GATTC                G^A[AT]TC
XhoII         208  GGATCC               [GA]^GATC[CT]
XmaCI         308  CCCGGG               C^CCGGG
XmaI          308  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI          852  CCGC                 CCGC(-3/-1)
              532  GCGG                
AcsI          601  AAATTT               [GA]^AATT[CT]
              795  GAATTC              
AfaI          269  GTAC                 GT^AC
              846  GTAC                
AlwI          209  GATCC                GGATC(4/5)
              208  GGATC               
ApoI          601  AAATTT               [GA]^AATT[CT]
              795  GAATTC              
BbvI          432  GCAGC                GCAGC(8/12)
              591  GCTGC               
BcnI          308  CCCGG                CC^[GC]GG
              309  CCGGG               
BsiYI         199  CCCCCTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              247  CCTGGAAACGG         
BslI          199  CCCCCTTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              247  CCTGGAAACGG         
BsmFI         410  GGGAC                GGGAC(10/14)
              657  GTCCC               
BstXI         166  CCAGGAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              660  CCAGGTTTATGG        
Fnu4HI        432  GCAGC                GC^[ACGT]GC
              591  GCTGC               
HinfI         243  GACTC                G^A[ACGT]TC
              372  GATTC               
ItaI          432  GCAGC                GC^[ACGT]GC
              591  GCTGC               
NciI          308  CCCGG                CC^[GC]GG
              309  CCGGG               
PstI           44  CTGCAG               CTGCA^G
              188  CTGCAG              
RsaI          269  GTAC                 GT^AC
              846  GTAC                
SfcI           44  CTGCAG               C^T[GA][CT]AG
              188  CTGCAG              
TseI          432  GCAGC                G^C[AT]GC
              591  GCTGC               
TspRI         321  CACTG                CA[GC]TG[ACGT][ACGT]^
              819  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AluI        BbsI        BmyI        BpuAI       BsaJI       
BseRI       Bsp1286I    BsrI        BssKI       BstNI       
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
HaeIII      MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NlaIII      NlaIV       PvuII       
Sau96I      ScrFI       SfaNI       Tru9I       Tsp509I     
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