Restriction map for pF1KSDA0024
 (Linear) (Six-base) MAPSORT of: KIAA0024.seq  Check: 1743  from: 1  to: 1419



KIAA0024.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1337    1419 
   Size:       1337      82
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1296    1328    1419 
   Size:       1296      32      91

  Fragments arranged by size: 

                1296      91      32
 
ApoI r'AATT_y

Cuts at:      0     363     968    1108    1419 
   Size:        363     605     140     311

  Fragments arranged by size: 

                 605     363     311     140
 
BanI G'GyrC_C

Cuts at:      0    1264    1419 
   Size:       1264     155
 
BanII G_rGCy'C

Cuts at:      0     602    1419 
   Size:        602     817
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     203    1325    1419 
   Size:        203    1122      94

  Fragments arranged by size: 

                1122     203      94
 
BclI T'GATC_A

Cuts at:      0      68     464     881    1419 
   Size:         68     396     417     538

  Fragments arranged by size: 

                 538     417     396      68
 
BfrBI ATG'CAT

Cuts at:      0      57     612    1419 
   Size:         57     555     807

  Fragments arranged by size: 

                 807     555      57
 
BglI GCCn_nnn'nGGC

Cuts at:      0     535    1419 
   Size:        535     884
 
BglII A'GATC_T

Cuts at:      0    1119    1280    1419 
   Size:       1119     161     139

  Fragments arranged by size: 

                1119     161     139
 
BlpI GC'TnA_GC

Cuts at:      0     136    1419 
   Size:        136    1283
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     747    1419 
   Size:        747     672
 
BmtI G_CTAG'C

Cuts at:      0     812    1419 
   Size:        812     607
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     235    1419 
   Size:        235    1184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     884    1108    1419 
   Size:        228     656     224     311

  Fragments arranged by size: 

                 656     311     228     224
 
Bpu10I CC'TnA_GC

Cuts at:      0      28    1419 
   Size:         28    1391
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     565    1419 
   Size:        565     854
 
BsaBI GATnn'nnATC

Cuts at:      0     404    1419 
   Size:        404    1015
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1419 
   Size:          4    1415
 
BsaWI w'CCGG_w

Cuts at:      0      62    1419 
   Size:         62    1357
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     607    1419 
   Size:        607     812
 
BseYI C'CCAG_C

Cuts at:      0     524     693    1061    1419 
   Size:        524     169     368     358

  Fragments arranged by size: 

                 524     368     358     169
 
BsiEI CG_ry'CG

Cuts at:      0     118    1419 
   Size:        118    1301
 
BsiHKAI G_wGCw'C

Cuts at:      0     329     602    1419 
   Size:        329     273     817

  Fragments arranged by size: 

                 817     329     273
 
BsmI GAATG_Cn'

Cuts at:      0    1380    1419 
   Size:       1380      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     329     602    1419 
   Size:        329     273     817

  Fragments arranged by size: 

                 817     329     273
 
BspEI T'CCGG_A

Cuts at:      0      62    1419 
   Size:         62    1357
 
BspHI T'CATG_A

Cuts at:      0     878    1419 
   Size:        878     541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     363    1419 
   Size:        363    1056
 
BsrDI GCAATG_nn'

Cuts at:      0     681    1419 
   Size:        681     738
 
BsrFI r'CCGG_y

Cuts at:      0     113     710    1419 
   Size:        113     597     709

  Fragments arranged by size: 

                 709     597     113
 
BstEII G'GTnAC_C

Cuts at:      0    1228    1419 
   Size:       1228     191
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     299     959    1419 
   Size:        299     660     460

  Fragments arranged by size: 

                 660     460     299
 
BstYI r'GATC_y

Cuts at:      0     399    1119    1280    1419 
   Size:        399     720     161     139

  Fragments arranged by size: 

                 720     399     161     139
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     663     698    1419 
   Size:        663      35     721

  Fragments arranged by size: 

                 721     663      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1037    1419 
   Size:       1037     382
 
EaeI y'GGCC_r

Cuts at:      0     115    1419 
   Size:        115    1304
 
EagI C'GGCC_G

Cuts at:      0     115    1419 
   Size:        115    1304
 
EarI CTCTTCn'nnn_

Cuts at:      0     361     607    1129    1419 
   Size:        361     246     522     290

  Fragments arranged by size: 

                 522     361     290     246
 
EcoICRI GAG'CTC

Cuts at:      0     600    1419 
   Size:        600     819
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     884    1108    1337    1419 
   Size:        228     656     224     229      82

  Fragments arranged by size: 

                 656     229     228     224      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      31     174     474    1419 
   Size:         31     143     300     945

  Fragments arranged by size: 

                 945     300     143      31
 
EcoRI G'AATT_C

Cuts at:      0     968    1419 
   Size:        968     451
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     945    1376    1419 
   Size:        945     431      43

  Fragments arranged by size: 

                 945     431      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     826     858    1106    1111    1138    1143    1359    1391 
   Size:        826      32     248       5      27       5     216      32

Cuts at:   1391    1419 
   Size:         28

  Fragments arranged by size: 

                 826     248     216      32      32      28      27       5
                   5
 
HindIII A'AGCT_T

Cuts at:      0     744    1419 
   Size:        744     675
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1389    1419 
   Size:       1389      30
 
MslI CAynn'nnrTG

Cuts at:      0     250    1419 
   Size:        250    1169
 
MspA1I CmG'CkG

Cuts at:      0     894    1419 
   Size:        894     525
 
NheI G'CTAG_C

Cuts at:      0     808    1419 
   Size:        808     611
 
NsiI A_TGCA'T

Cuts at:      0      59     614    1419 
   Size:         59     555     805

  Fragments arranged by size: 

                 805     555      59
 
NspI r_CATG'y

Cuts at:      0    1339    1419 
   Size:       1339      80
 
PfoI T'CCnGG_A

Cuts at:      0    1085    1363    1419 
   Size:       1085     278      56

  Fragments arranged by size: 

                1085     278      56
 
PstI C_TGCA'G

Cuts at:      0     797    1419 
   Size:        797     622
 
PvuII CAG'CTG

Cuts at:      0     894    1419 
   Size:        894     525
 
SacI G_AGCT'C

Cuts at:      0     602    1419 
   Size:        602     817
 
SapI GCTCTTCn'nnn_

Cuts at:      0     607    1419 
   Size:        607     812
 
ScaI AGT'ACT

Cuts at:      0    1012    1419 
   Size:       1012     407
 
SfcI C'TryA_G

Cuts at:      0     793    1269    1419 
   Size:        793     476     150

  Fragments arranged by size: 

                 793     476     150
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     535    1419 
   Size:        535     884
 
SphI G_CATG'C

Cuts at:      0    1339    1419 
   Size:       1339      80
 
StuI AGG'CCT

Cuts at:      0     538    1419 
   Size:        538     881
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1053    1344    1419 
   Size:       1053     291      75

  Fragments arranged by size: 

                1053     291      75
 
TatI w'GTAC_w

Cuts at:      0     161    1010    1419 
   Size:        161     849     409

  Fragments arranged by size: 

                 849     409     161
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     308     340    1257    1289    1419 
   Size:        308      32     917      32     130

  Fragments arranged by size: 

                 917     308     130      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1283    1419 
   Size:       1283     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     910    1419 
   Size:        910     509
 

 Enzymes that do cut:

     AcuI     AloI     ApoI     BanI    BanII     BbsI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI   BstEII
    BstXI    BstYI    CspCI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI    EcoRI     FalI    Hin4I  HindIII     MmeI     MslI
   MspA1I     NheI     NsiI     NspI     PfoI     PstI    PvuII     SacI
     SapI     ScaI     SfcI     SfiI     SphI     StuI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI Bme1580I    BmgBI    BpuEI    BsaAI    BsaXI     BsgI
    BsiWI    BsmBI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI
     EciI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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