Restriction map for pF1KSDA0023
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AccIII      AflIII      AgeI        AhdI        ApaI        
AscI        AspEI       AspI        AviII       AvrII       
BbrPI       BglII       BlnI        BsaAI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HpaI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NotI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XmaCI       XmaI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI         1099  GTAGAC               GT^[AC][GT]AC
AccII        6214  CGCG                 CG^CG
AclI         3309  AACGTT               AA^CGTT
AflII        3451  CTTAAG               C^TTAAG
Alw44I       3200  GTGCAC               G^TGCAC
Aor51HI        60  AGCGCT               AGC^GCT
ApaLI        3200  GTGCAC               G^TGCAC
AspHI        3200  GTGCAC               G[AT]GC[AT]^C
BbeI         2411  GGCGCC               GGCGC^C
BbvCI        5402  CCTCAGC              CCTCAGC(-5/-2)
BciVI         980  GTATCC               GTATCC(6/5)
BclI          999  TGATCA               T^GATCA
BfrI         3451  CTTAAG               C^TTAAG
BsaWI        3478  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      3200  GTGCAC               G[AT]GC[AT]^C
BstUI        6214  CGCG                 CG^CG
BstXI        5805  CCAAGGCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III       60  AGCGCT               AGC^GCT
EcoNI        3754  CCTCCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          999  TGATCA               T^GATCA
KasI         2411  GGCGCC               G^GCGCC
MvnI         6214  CGCG                 CG^CG
NarI         2411  GGCGCC               GG^CGCC
NheI         3136  GCTAGC               G^CTAGC
NruI         6213  TCGCGA               TCG^CGA
PaeR7I        114  CTCGAG               C^TCGAG
PshAI        4716  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3309  AACGTT               AA^CGTT
SfoI         2411  GGCGCC               GGC^GCC
StuI         5120  AGGCCT               AGG^CCT
XhoI          114  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII        2967  GACGTC               GACGT^C
             4029  GACGTC              
AseI         1006  ATTAAT               AT^TAAT
             1210  ATTAAT              
AsnI         1006  ATTAAT               AT^TAAT
             1210  ATTAAT              
Asp700I      3932  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4555  GAACCTGTTC          
AvaI           28  CCCGAG               C^[CT]CG[GA]G
              114  CTCGAG              
BcgI            9  CGAGATGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5963  GCAAAGTGTTCG        
BsaBI        2059  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2476  GATCAGCATC          
BsoBI          28  CCCGAG               C^[CT]CG[GA]G
              114  CTCGAG              
BsrBI        6095  CCGCTC               CCGCTC(-3/-3)
               31  GAGCGG              
DrdI         2235  GACCACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4456  GACAGTGAGGTC        
HaeII          60  AGCGCT               [GA]GCGC^[CT]
             2411  GGCGCC              
HgaI         3694  GACGC                GACGC(5/10)
             1606  GCGTC               
HincII       1753  GTCAAC               GT[CT]^[GA]AC
             3399  GTTGAC              
HindII       1753  GTCAAC               GT[CT]^[GA]AC
             3399  GTTGAC              
MamI         2059  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2476  GATCAGCATC          
NcoI          488  CCATGG               C^CATGG
             1085  CCATGG              
PshBI        1006  ATTAAT               AT^TAAT
             1210  ATTAAT              
XbaI         2460  TCTAGA               T^CTAGA
             4488  TCTAGA              
XcmI         6166  CCATTAAAAGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6250  CCAACAGACTTCTGG     
XmnI         3932  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4555  GAACCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

Acc65I      AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp718I     AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SpeI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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