Restriction map for pF1KSDA0023
 (Linear) (Six-base) MAPSORT of: KIAA0023.seq  Check: 338  from: 1  to: 6279



KIAA0023.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1395    2519    4695    6279 
   Size:       1395    1124    2176    1584

  Fragments arranged by size: 

                2176    1584    1395    1124
 
AatII G_ACGT'C

Cuts at:      0    2971    4033    6279 
   Size:       2971    1062    2246

  Fragments arranged by size: 

                2971    2246    1062
 
AccI GT'mk_AC

Cuts at:      0    1100    6279 
   Size:       1100    5179
 
Acc65I G'GTAC_C

Cuts at:      0     748    4843    5077    5398    6279 
   Size:        748    4095     234     321     881

  Fragments arranged by size: 

                4095     881     748     321     234
 
AclI AA'CG_TT

Cuts at:      0    3310    6279 
   Size:       3310    2969
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2002    2383    2596    3104    3491    5154    5438    5577 
   Size:       2002     381     213     508     387    1663     284     139

Cuts at:   5577    6061    6279 
   Size:        484     218

  Fragments arranged by size: 

                2002    1663     508     484     387     381     284     218
                 213     139
 
AfeI AGC'GCT

Cuts at:      0      62    6279 
   Size:         62    6217
 
AflII C'TTAA_G

Cuts at:      0    3451    6279 
   Size:       3451    2828
 
AleI CACnn'nnGTG

Cuts at:      0    1681    4951    6279 
   Size:       1681    3270    1328

  Fragments arranged by size: 

                3270    1681    1328
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1699    3257    6070    6279 
   Size:       1699    1558    2813     209

  Fragments arranged by size: 

                2813    1699    1558     209
 
AlwNI CAG_nnn'CTG

Cuts at:      0     958    1338    1363    1543    2068    2484    2946    3412 
   Size:        958     380      25     180     525     416     462     466

Cuts at:   3412    3598    4732    4753    4933    5029    6279 
   Size:        186    1134      21     180      96    1250

  Fragments arranged by size: 

                1250    1134     958     525     466     462     416     380
                 186     180     180      96      25      21
 
ApaLI G'TGCA_C

Cuts at:      0    3200    6279 
   Size:       3200    3079
 
ApoI r'AATT_y

Cuts at:      0     274    2405    2431    3444    6279 
   Size:        274    2131      26    1013    2835

  Fragments arranged by size: 

                2835    2131    1013     274      26
 
AseI AT'TA_AT

Cuts at:      0    1007    1211    6279 
   Size:       1007     204    5068

  Fragments arranged by size: 

                5068    1007     204
 
AvaI C'yCGr_G

Cuts at:      0      28     114    6279 
   Size:         28      86    6165

  Fragments arranged by size: 

                6165      86      28
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     740     773    6279 
   Size:        740      33    5506

  Fragments arranged by size: 

                5506     740      33
 
BamHI G'GATC_C

Cuts at:      0    2376    4195    5668    5887    6279 
   Size:       2376    1819    1473     219     392

  Fragments arranged by size: 

                2376    1819    1473     392     219
 
BanI G'GyrC_C

Cuts at:      0     748    2411    2507    3279    3825    4843    5077    5254 
   Size:        748    1663      96     772     546    1018     234     177

Cuts at:   5254    5398    5907    6279 
   Size:        144     509     372

  Fragments arranged by size: 

                1663    1018     772     748     546     509     372     234
                 177     144      96
 
BanII G_rGCy'C

Cuts at:      0     604     878    1434    1496    4094    5953    6279 
   Size:        604     274     556      62    2598    1859     326

  Fragments arranged by size: 

                2598    1859     604     556     326     274      62
 
BbeI G_GCGC'C

Cuts at:      0    2415    6279 
   Size:       2415    3864
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     144     750    2602    2836    2909    3626    5294    6279 
   Size:        144     606    1852     234      73     717    1668     985

  Fragments arranged by size: 

                1852    1668     985     717     606     234     144      73
            
 
BbvCI CC'TCA_GC

Cuts at:      0    5403    6279 
   Size:       5403     876
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      32    5952    5986    6279 
   Size:         32    5920      34     293

  Fragments arranged by size: 

                5920     293      34      32
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     991    6279 
   Size:        991    5288
 
BclI T'GATC_A

Cuts at:      0     999    6279 
   Size:        999    5280
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3235    3277    3462    5948    6279 
   Size:       3235      42     185    2486     331

  Fragments arranged by size: 

                3235    2486     331     185      42
 
BlpI GC'TnA_GC

Cuts at:      0    2393    4874    4899    6279 
   Size:       2393    2481      25    1380

  Fragments arranged by size: 

                2481    2393    1380      25
 
Bme1580I G_kGCm'C

Cuts at:      0    1946    2164    3204    4043    4289    5983    6279 
   Size:       1946     218    1040     839     246    1694     296

  Fragments arranged by size: 

                1946    1694    1040     839     296     246     218
 
BmgBI CAC'GTC

Cuts at:      0    4799    6279 
   Size:       4799    1480
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1496    1933    2934    3038    3388    3400    5609    6279 
   Size:       1496     437    1001     104     350      12    2209     670

  Fragments arranged by size: 

                2209    1496    1001     670     437     350     104      12
            
 
BmtI G_CTAG'C

Cuts at:      0    3140    6279 
   Size:       3140    3139
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2408    6047    6279 
   Size:       2408    3639     232

  Fragments arranged by size: 

                3639    2408     232
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618     788    1048    1142    1964    2840    2968    3938 
   Size:        618     170     260      94     822     876     128     970

Cuts at:   3938    4773    5262    5804    5840    6279 
   Size:        835     489     542      36     439

  Fragments arranged by size: 

                 970     876     835     822     618     542     489     439
                 260     170     128      94      36
 
Bpu10I CC'TnA_GC

Cuts at:      0    1922    5052    5403    6279 
   Size:       1922    3130     351     876

  Fragments arranged by size: 

                3130    1922     876     351
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318    1307    2305    3137    3311    4725    5247    6279 
   Size:        318     989     998     832     174    1414     522    1032

  Fragments arranged by size: 

                1414    1032     998     989     832     522     318     174
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2227    3440    4470    6279 
   Size:       2227    1213    1030    1809

  Fragments arranged by size: 

                2227    1809    1213    1030
 
BsaBI GATnn'nnATC

Cuts at:      0    2063    2480    6279 
   Size:       2063     417    3799

  Fragments arranged by size: 

                3799    2063     417
 
BsaHI Gr'CG_yC

Cuts at:      0    2412    2968    4030    6279 
   Size:       2412     556    1062    2249

  Fragments arranged by size: 

                2412    2249    1062     556
 
BsaWI w'CCGG_w

Cuts at:      0    3478    6279 
   Size:       3478    2801
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     437     467    1082    1112    1134    1164    4486    4516 
   Size:        437      30     615      30      22      30    3322      30

Cuts at:   4516    5372    5402    5780    5810    5951    5981    6279 
   Size:        856      30     378      30     141      30     298

  Fragments arranged by size: 

                3322     856     615     437     378     298     141      30
                  30      30      30      30      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1308    2104    2185    4507    4514    5801    6092    6279 
   Size:       1308     796      81    2322       7    1287     291     187

  Fragments arranged by size: 

                2322    1308    1287     796     291     187      81       7
            
 
BseYI C'CCAG_C

Cuts at:      0    3885    4016    4508    4568    4641    5067    5928    6279 
   Size:       3885     131     492      60      73     426     861     351

  Fragments arranged by size: 

                3885     861     492     426     351     131      73      60
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     876    3361    3635    4211    4766    6279 
   Size:        876    2485     274     576     555    1513

  Fragments arranged by size: 

                2485    1513     876     576     555     274
 
BsiHKAI G_wGCw'C

Cuts at:      0    3204    6279 
   Size:       3204    3075
 
BsmI GAATG_Cn'

Cuts at:      0    2681    3697    5914    6229    6279 
   Size:       2681    1016    2217     315      50

  Fragments arranged by size: 

                2681    2217    1016     315      50
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     806    1613    6279 
   Size:        806     807    4666

  Fragments arranged by size: 

                4666     807     806
 
Bsp1286I G_dGCh'C

Cuts at:      0     604     878    1434    1496    1946    2164    3204    4043 
   Size:        604     274     556      62     450     218    1040     839

Cuts at:   4043    4094    4289    5953    5983    6279 
   Size:         51     195    1664      30     296

  Fragments arranged by size: 

                1664    1040     839     604     556     450     296     274
                 218     195      62      51      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1395    2519    3807    4695    6279 
   Size:       1395    1124    1288     888    1584

  Fragments arranged by size: 

                1584    1395    1288    1124     888
 
BsrBI CCG'CTC

Cuts at:      0      33    6097    6279 
   Size:         33    6064     182

  Fragments arranged by size: 

                6064     182      33
 
BsrDI GCAATG_nn'

Cuts at:      0    1397    1718    2724    6279 
   Size:       1397     321    1006    3555

  Fragments arranged by size: 

                3555    1397    1006     321
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     958    1363    3070    4627    6279 
   Size:        958     405    1707    1557    1652

  Fragments arranged by size: 

                1707    1652    1557     958     405
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    5812    6279 
   Size:       5812     467
 
BstYI r'GATC_y

Cuts at:      0    2064    2376    2845    4195    5668    5887    6124    6279 
   Size:       2064     312     469    1350    1473     219     237     155

  Fragments arranged by size: 

                2064    1473    1350     469     312     237     219     155
            
 
Bsu36I CC'TnA_GG

Cuts at:      0      92    3759    4044    6279 
   Size:         92    3667     285    2235

  Fragments arranged by size: 

                3667    2235     285      92
 
BtgI C'CryG_G

Cuts at:      0     488     572    1085    3017    4649    6279 
   Size:        488      84     513    1932    1632    1630

  Fragments arranged by size: 

                1932    1632    1630     513     488      84
 
BtsI GCAGTG_nn'

Cuts at:      0     629    2849    2996    3644    4592    4931    5154    5981 
   Size:        629    2220     147     648     948     339     223     827

Cuts at:   5981    6279 
   Size:        298

  Fragments arranged by size: 

                2220     948     827     648     629     339     298     223
                 147
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1097    1132    4785    4820    5820    5855    6279 
   Size:       1097      35    3653      35    1000      35     424

  Fragments arranged by size: 

                3653    1097    1000     424      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2601    3106    3396    6279 
   Size:       2601     505     290    2883

  Fragments arranged by size: 

                2883    2601     505     290
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2241    4462    6279 
   Size:       2241    2221    1817

  Fragments arranged by size: 

                2241    2221    1817
 
EaeI y'GGCC_r

Cuts at:      0    2819    3269    3954    4757    5331    5523    5651    6017 
   Size:       2819     450     685     803     574     192     128     366

Cuts at:   6017    6132    6279 
   Size:        115     147

  Fragments arranged by size: 

                2819     803     685     574     450     366     192     147
                 128     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     569    1151    1809    2114    2578    4412    5544    5571 
   Size:        569     582     658     305     464    1834    1132      27

Cuts at:   5571    6279 
   Size:        708

  Fragments arranged by size: 

                1834    1132     708     658     582     569     464     305
                  27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2611    6279 
   Size:       2611    3668
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618     788    1048    1142    1964    2002    2383    2596 
   Size:        618     170     260      94     822      38     381     213

Cuts at:   2596    2840    2968    3104    3491    3938    4773    5154    5262 
   Size:        244     128     136     387     447     835     381     108

Cuts at:   5262    5438    5577    5804    5840    6061    6279 
   Size:        176     139     227      36     221     218

  Fragments arranged by size: 

                 835     822     618     447     387     381     381     260
                 244     227     221     218     213     176     170     139
                 136     128     108      94      38      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3758    6279 
   Size:       3758    2521
 
EcoO109I rG'GnC_Cy

Cuts at:      0     788    1540    3000    4656    4750    6011    6270    6279 
   Size:        788     752    1460    1656      94    1261     259       9

  Fragments arranged by size: 

                1656    1460    1261     788     752     259      94       9
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1740    1774    2413    3740    6279 
   Size:       1740      34     639    1327    2539

  Fragments arranged by size: 

                2539    1740    1327     639      34
 
HaeII r_GCGC'y

Cuts at:      0      64    2415    6279 
   Size:         64    2351    3864

  Fragments arranged by size: 

                3864    2351      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     891     923    1134    1166    1962    1994    2577    2609 
   Size:        891      32     211      32     796      32     583      32

Cuts at:   2609    3258    3290    3685    3717    4486    4518    5372    5404 
   Size:        649      32     395      32     769      32     854      32

Cuts at:   5404    6109    6141    6279 
   Size:        705      32     138

  Fragments arranged by size: 

                 891     854     796     769     705     649     583     395
                 211     138      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1755    3401    6279 
   Size:       1755    1646    2878

  Fragments arranged by size: 

                2878    1755    1646
 
HindIII A'AGCT_T

Cuts at:      0     427    3503    5450    6279 
   Size:        427    3076    1947     829

  Fragments arranged by size: 

                3076    1947     829     427
 
KasI G'GCGC_C

Cuts at:      0    2411    6279 
   Size:       2411    3868
 
KpnI G_GTAC'C

Cuts at:      0     752    4847    5081    5402    6279 
   Size:        752    4095     234     321     877

  Fragments arranged by size: 

                4095     877     752     321     234
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1541    2096    4354    6058    6279 
   Size:        158    1383     555    2258    1704     221

  Fragments arranged by size: 

                2258    1704    1383     555     221     158
 
MscI TGG'CCA

Cuts at:      0    2821    3271    3956    5333    5525    6019    6134    6279 
   Size:       2821     450     685    1377     192     494     115     145

  Fragments arranged by size: 

                2821    1377     685     494     450     192     145     115
            
 
MslI CAynn'nnrTG

Cuts at:      0    1681    2423    4951    6279 
   Size:       1681     742    2528    1328

  Fragments arranged by size: 

                2528    1681    1328     742
 
MspA1I CmG'CkG

Cuts at:      0     618     890    1354    1627    1822    1984    3241    3286 
   Size:        618     272     464     273     195     162    1257      45

Cuts at:   3286    3532    4315    4402    4732    5029    5407    5989    6279 
   Size:        246     783      87     330     297     378     582     290

  Fragments arranged by size: 

                1257     783     618     582     464     378     330     297
                 290     273     272     246     195     162      87      45
            
 
NarI GG'CG_CC

Cuts at:      0    2412    6279 
   Size:       2412    3867
 
NcoI C'CATG_G

Cuts at:      0     488    1085    6279 
   Size:        488     597    5194

  Fragments arranged by size: 

                5194     597     488
 
NheI G'CTAG_C

Cuts at:      0    3136    6279 
   Size:       3136    3143
 
NruI TCG'CGA

Cuts at:      0    6215    6279 
   Size:       6215      64
 
PasI CC'CwG_GG

Cuts at:      0    5089    5893    6279 
   Size:       5089     804     386

  Fragments arranged by size: 

                5089     804     386
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     291    1543    2946    5074    5863    6279 
   Size:        291    1252    1403    2128     789     416

  Fragments arranged by size: 

                2128    1403    1252     789     416     291
 
PpuMI rG'GwC_Cy

Cuts at:      0     788    4656    6011    6270    6279 
   Size:        788    3868    1355     259       9

  Fragments arranged by size: 

                3868    1355     788     259       9
 
PshAI GACnn'nnGTC

Cuts at:      0    4720    6279 
   Size:       4720    1559
 
PsiI TTA'TAA

Cuts at:      0    2363    6279 
   Size:       2363    3916
 
PspXI vC'TCGA_Gb

Cuts at:      0     114    6279 
   Size:        114    6165
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    5265    5297    5463    5495    6279 
   Size:       5265      32     166      32     784

  Fragments arranged by size: 

                5265     784     166      32      32
 
PstI C_TGCA'G

Cuts at:      0     783    1353    1359    1605    4578    4599    4632    4827 
   Size:        783     570       6     246    2973      21      33     195

Cuts at:   4827    5988    6279 
   Size:       1161     291

  Fragments arranged by size: 

                2973    1161     783     570     291     246     195      33
                  21       6
 
PvuII CAG'CTG

Cuts at:      0     618     890    1354    1627    1822    3241    3286    3532 
   Size:        618     272     464     273     195    1419      45     246

Cuts at:   3532    4315    4732    5029    5407    5989    6279 
   Size:        783     417     297     378     582     290

  Fragments arranged by size: 

                1419     783     618     582     464     417     378     297
                 290     273     272     246     195      45
 
SanDI GG'GwC_CC

Cuts at:      0     788    4656    6011    6270    6279 
   Size:        788    3868    1355     259       9

  Fragments arranged by size: 

                3868    1355     788     259       9
 
ScaI AGT'ACT

Cuts at:      0    1299    4608    5505    6279 
   Size:       1299    3309     897     774

  Fragments arranged by size: 

                3309    1299     897     774
 
SexAI A'CCwGG_T

Cuts at:      0    2597    3083    3102    6279 
   Size:       2597     486      19    3177

  Fragments arranged by size: 

                3177    2597     486      19
 
SfcI C'TryA_G

Cuts at:      0     779    1349    1355    1601    3374    4310    4574    4595 
   Size:        779     570       6     246    1773     936     264      21

Cuts at:   4595    4628    4823    5984    6279 
   Size:         33     195    1161     295

  Fragments arranged by size: 

                1773    1161     936     779     570     295     264     246
                 195      33      21       6
 
SfoI GGC'GCC

Cuts at:      0    2413    6279 
   Size:       2413    3866
 
SmlI C'TyrA_G

Cuts at:      0     114     297    1322    2284    3152    3326    3451    4740 
   Size:        114     183    1025     962     868     174     125    1289

Cuts at:   4740    5262    6279 
   Size:        522    1017

  Fragments arranged by size: 

                1289    1025    1017     962     868     522     183     174
                 125     114
 
SpeI A'CTAG_T

Cuts at:      0    4009    4246    4609    6279 
   Size:       4009     237     363    1670

  Fragments arranged by size: 

                4009    1670     363     237
 
StuI AGG'CCT

Cuts at:      0    5122    6279 
   Size:       5122    1157
 
StyI C'CwwG_G

Cuts at:      0     104     252     488    1085    5601    5805    6068    6129 
   Size:        104     148     236     597    4516     204     263      61

Cuts at:   6129    6279 
   Size:        150

  Fragments arranged by size: 

                4516     597     263     236     204     150     148     104
                  61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     863    1980    3407    5194    6279 
   Size:        863    1117    1427    1787    1085

  Fragments arranged by size: 

                1787    1427    1117    1085     863
 
TatI w'GTAC_w

Cuts at:      0    1297    4606    5503    6279 
   Size:       1297    3309     897     776

  Fragments arranged by size: 

                3309    1297     897     776
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469     724     756    2698    2730    2740    2772 
   Size:        437      32     255      32    1942      32      10      32

Cuts at:   2772    3107    3139    4311    4343    5780    5812    5951    5983 
   Size:        335      32    1172      32    1437      32     139      32

Cuts at:   5983    6279 
   Size:        296

  Fragments arranged by size: 

                1942    1437    1172     437     335     296     255     139
                  32      32      32      32      32      32      32      32
                  10
 
XbaI T'CTAG_A

Cuts at:      0    2460    4488    6279 
   Size:       2460    2028    1791

  Fragments arranged by size: 

                2460    2028    1791
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    6173    6257    6279 
   Size:       6173      84      22

  Fragments arranged by size: 

                6173      84      22
 
XhoI C'TCGA_G

Cuts at:      0     114    6279 
   Size:        114    6165
 
XmnI GAAnn'nnTTC

Cuts at:      0    3936    4559    6279 
   Size:       3936     623    1720

  Fragments arranged by size: 

                3936    1720     623
 
ZraI GAC'GTC

Cuts at:      0    2969    4031    6279 
   Size:       2969    1062    2248

  Fragments arranged by size: 

                2969    2248    1062
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NheI     NruI
     PasI    PflMI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
    PvuII    SanDI     ScaI    SexAI     SfcI     SfoI     SmlI     SpeI
     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmnI     ZraI

 Enzymes that do not cut: 

   AflIII     AgeI     AhdI     AloI     ApaI     AscI    AsiSI    AvrII
    BfrBI    BglII    BsaAI    BsiEI    BsiWI    BspEI    BspHI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI
     DraI     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NotI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI   PspOMI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
  Tth111I     XmaI
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