Restriction map for pF1KSDA0019
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AccI        AclI        AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbsI        BglII       BlnI        BpuAI       BsaAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraIII      Eam1105I    Eco47III    Eco81I      FspI        
HpaI        KpnI        KspI        MfeI        MluI        
MunI        NdeI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AatII        1474  GACGTC               GACGT^C
AccII        1539  CGCG                 CG^CG
AccIII       2040  TCCGGA               T^CCGGA
AflII         745  CTTAAG               C^TTAAG
AlwI          345  GATCC                GGATC(4/5)
BalI         1193  TGGCCA               TGG^CCA
BanI         1218  GGCGCC               G^G[CT][GA]CC
BbeI         1218  GGCGCC               GGCGC^C
BbvCI        1615  GCTGAGG              CCTCAGC(-5/-2)
BcgI          603  CGAGGAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1795  GTATCC               GTATCC(6/5)
BclI         2165  TGATCA               T^GATCA
BfrI          745  CTTAAG               C^TTAAG
BlpI         1768  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1768  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1087  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2040  TCCGGA               [AT]^CCGG[AT]
BseAI        2040  TCCGGA               T^CCGGA
BsgI           83  GTGCAG               GTGCAG(16/14)
BspEI        2040  TCCGGA               T^CCGGA
BspHI         711  TCATGA               T^CATGA
BsrBI        1212  GAGCGG               CCGCTC(-3/-3)
BssSI        1234  CACGAG               CACGAG(-5/-1)
BstAPI       2380  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2144  GGTCACC              G^GT[ACGT]ACC
BstPI        2144  GGTCACC              G^GT[ACGT]ACC
BstUI        1539  CGCG                 CG^CG
BstXI        1851  CCAGCTAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         344  AGATCC               [GA]^GATC[CT]
CelII        1768  GCTGAGC              GC^T[ACGT]AGC
DraI         1813  TTTAAA               TTT^AAA
DrdI          433  GACCTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          634  CTCTTC               CTCTTC(1/4)
EcoNI         978  CCTGGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2144  GGTCACC              G^GT[ACGT]ACC
EcoRI           3  GAATTC               G^AATTC
EcoT22I       658  ATGCAT               ATGCA^T
FbaI         2165  TGATCA               T^GATCA
FseI         1177  GGCCGGCC             GGCCGG^CC
KasI         1218  GGCGCC               G^GCGCC
Ksp632I       634  CTCTTC               CTCTTC(1/4)
MamI         1087  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          344  AGATCC               [GA]^GATC[CT]
MluNI        1193  TGGCCA               TGG^CCA
MroI         2040  TCCGGA               T^CCGGA
MscI         1193  TGGCCA               TGG^CCA
MslI          591  CATGTATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1539  CGCG                 CG^CG
NaeI         1178  GCCGGC               GCC^GGC
NarI         1218  GGCGCC               GG^CGCC
NgoMIV       1178  GCCGGC               G^CCGGC
NsiI          658  ATGCAT               ATGCA^T
PstI         2333  CTGCAG               CTGCA^G
PvuII        1400  CAGCTG               CAG^CTG
RcaI          711  TCATGA               T^CATGA
SacI          163  GAGCTC               GAGCT^C
SexAI        1074  ACCTGGT              A^CC[AT]GGT
SfoI         1218  GGCGCC               GGC^GCC
SphI         1734  GCATGC               GCATG^C
XcmI         1499  CCAAATACACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         344  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BglI          613  GCCTTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1104  GCCCTTGGGGC         
BpmI          307  CTCCAG               CTGGAG(16/14)
             2426  CTCCAG              
BsaI          973  GAGACC               GGTCTC(1/5)
             2004  GAGACC              
BsiEI        1180  CGGCCG               CG[GA][CT]^CG
             2375  CGGCCG              
BsmBI        1476  CGTCTC               CGTCTC(1/5)
             2236  GAGACG              
BsrFI        1178  GCCGGC               [GA]^CCGG[CT]
             2373  ACCGGC              
Cfr10I       1178  GCCGGC               [GA]^CCGG[CT]
             2373  ACCGGC              
EagI         1180  CGGCCG               C^GGCCG
             2375  CGGCCG              
EclXI        1180  CGGCCG               C^GGCCG
             2375  CGGCCG              
Eco52I       1180  CGGCCG               C^GGCCG
             2375  CGGCCG              
HincII        442  GTCAAC               GT[CT]^[GA]AC
             2107  GTTGAC              
HindII        442  GTCAAC               GT[CT]^[GA]AC
             2107  GTTGAC              
NheI         1875  GCTAGC               G^CTAGC
             2266  GCTAGC              
NspI          651  ACATGC               [GA]CATG^[CT]
             1734  GCATGC              
PflMI        1069  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2149  CCAAAGAATGG         
PleI         1983  GACTC                GAGTC(4/5)
             2470  GAGTC               
PpuMI         371  GGGACCT              [GA]G^G[AT]CC[CT]
             1560  AGGTCCT             
TaqI         1524  TCGA                 T^CGA
             2088  TCGA                
Tsp45I        130  GTCAC                ^GT[GC]AC
             2145  GTCAC               
TthHB8I      1524  TCGA                 T^CGA
             2088  TCGA                
Van91I       1069  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2149  CCAAAGAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwNI       ApaI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoRV       EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XmnI        
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