Restriction map for pF1KSDA0019
 (Circular) (Six-base) MAPSORT of: pF1KSDA0019.seq  Check: 6047  from: 1  to: 5621



pF1KSDA0019  5621 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AatII G_ACGT'C

Cuts at:   1604    1604 
   Size:       5621
 
AccI GT'mk_AC

Cuts at:   2659    2659 
   Size:       5621
 
Acc65I G'GTAC_C

Cuts at:   2637    2637 
   Size:       5621
 
AclI AA'CG_TT

Cuts at:   5461    5461 
   Size:       5621
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1135    1240    3446    3878    4625    4893    1135 
   Size:        105    2206     432     747     268    1863

  Fragments arranged by size: 

                2206    1863     747     432     268     105
 
AflII C'TTAA_G

Cuts at:    871     871 
   Size:       5621
 
AflIII A'CryG_T

Cuts at:   2973    4077    5054    2973 
   Size:       1104     977    3540

  Fragments arranged by size: 

                3540    1104     977
 
AgeI A'CCGG_T

Cuts at:   4009    4009 
   Size:       5621
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    992    1024     992 
   Size:         32    5589
 
AlwNI CAG_nnn'CTG

Cuts at:   1201    1690    2467    2926    4493    1201 
   Size:        489     777     459    1567    2329

  Fragments arranged by size: 

                2329    1567     777     489     459
 
ApaI G_GGCC'C

Cuts at:    459     750    2546     459 
   Size:        291    1796    3534

  Fragments arranged by size: 

                3534    1796     291
 
ApaLI G'TGCA_C

Cuts at:   4391    4391 
   Size:       5621
 
ApoI r'AATT_y

Cuts at:    129     413     856    1087    2625    4974    5230     129 
   Size:        284     443     231    1538    2349     256     520

  Fragments arranged by size: 

                2349    1538     520     443     284     256     231
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5621
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5621
 
AvaI C'yCGr_G

Cuts at:   1399    1881    1994    2384    2641    1399 
   Size:        482     113     390     257    4379

  Fragments arranged by size: 

                4379     482     390     257     113
 
BamHI G'GATC_C

Cuts at:   2646    2646 
   Size:       5621
 
BanI G'GyrC_C

Cuts at:   1344    2637    3051    3299    3334    1344 
   Size:       1293     414     248      35    3631

  Fragments arranged by size: 

                3631    1293     414     248      35
 
BanII G_rGCy'C

Cuts at:    293     459     750    1315    1762    1855    2546    2635    3665 
   Size:        166     291     565     447      93     691      89    1030

Cuts at:   3665     293 
   Size:       2249

  Fragments arranged by size: 

                2249    1030     691     565     447     291     166      93
                  89
 
BbeI G_GCGC'C

Cuts at:   1348    3055    3303    1348 
   Size:       1707     248    3666

  Fragments arranged by size: 

                3666    1707     248
 
BbvCI CC'TCA_GC

Cuts at:   1742    1742 
   Size:       5621
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    718     752     718 
   Size:         34    5587
 
BciVI GTATCCnnnnn_n'

Cuts at:   1932    3519    4280    5621    1932 
   Size:       1587     761    1341    1932

  Fragments arranged by size: 

                1932    1587    1341     761
 
BclI T'GATC_A

Cuts at:   2291    3141    2291 
   Size:        850    4771
 
BfrBI ATG'CAT

Cuts at:    786     786 
   Size:       5621
 
BglI GCCn_nnn'nGGC

Cuts at:    745    1236     745 
   Size:        491    5130
 
BglII A'GATC_T

Cuts at:   3136    5173    3136 
   Size:       2037    3584
 
BlpI GC'TnA_GC

Cuts at:   1895    2733    1895 
   Size:        838    4783
 
Bme1580I G_kGCm'C

Cuts at:    459     750    1580    2546    3246    3339    4395     459 
   Size:        291     830     966     700      93    1056    1685

  Fragments arranged by size: 

                1685    1056     966     830     700     291      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2833    2914    3249    5393    2833 
   Size:         81     335    2144    3061

  Fragments arranged by size: 

                3061    2144     335      81
 
BmtI G_CTAG'C

Cuts at:   2005    2396    2005 
   Size:        391    5230
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3988    3988 
   Size:       5621
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    418    2537     418 
   Size:       2119    3502
 
Bpu10I CC'TnA_GC

Cuts at:   1742    1742 
   Size:       5621
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    487     840     875    1299    2797    3676    4168    4466     487 
   Size:        353      35     424    1498     879     492     298    1642

  Fragments arranged by size: 

                1642    1498     879     492     424     353     298      35
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1093    2124    5325    1093 
   Size:       1031    3201    1389

  Fragments arranged by size: 

                3201    1389    1031
 
BsaAI yAC'GTr

Cuts at:   2613    2974    2613 
   Size:        361    5260
 
BsaBI GATnn'nnATC

Cuts at:      6    1217    3135    5172       6 
   Size:       1211    1918    2037     455

  Fragments arranged by size: 

                2037    1918    1211     455
 
BsaHI Gr'CG_yC

Cuts at:   1268    1345    1396    1601    3052    3300    4002    5498    1268 
   Size:         77      51     205    1451     248     702    1496    1391

  Fragments arranged by size: 

                1496    1451    1391     702     248     205      77      51
            
 
BsaWI w'CCGG_w

Cuts at:   2166    2810    3331    4009    4283    4430    4841    2166 
   Size:        644     521     678     274     147     411    2946

  Fragments arranged by size: 

                2946     678     644     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    992    1022    1351    1381     992 
   Size:         30     329      30    5232

  Fragments arranged by size: 

                5232     329      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    298     301     703    1308    1450    1453    1837     298 
   Size:          3     402     605     142       3     384    4082

  Fragments arranged by size: 

                4082     605     402     384     142       3       3
 
BseYI C'CCAG_C

Cuts at:   1876    1976    2245    3046    3804    4381    4877    1876 
   Size:        100     269     801     758     577     496    2620

  Fragments arranged by size: 

                2620     801     758     577     496     269     100
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    230     230 
   Size:       5621
 
BsiEI CG_ry'CG

Cuts at:    104    1309    2504    3163    3209    4417     104 
   Size:       1205    1195     659      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1205    1195     659      46
 
BsiHKAI G_wGCw'C

Cuts at:    293     528    1367    1410    2635    3413    3603    4395     293 
   Size:        235     839      43    1225     778     190     792    1519

  Fragments arranged by size: 

                1519    1225     839     792     778     235     190      43
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1608    2356    1608 
   Size:        748    4873
 
Bsp1286I G_dGCh'C

Cuts at:    293     459     528     750    1315    1367    1410    1580    1762 
   Size:        166      69     222     565      52      43     170     182

Cuts at:   1762    1855    2546    2635    3246    3339    3413    3603    3665 
   Size:         93     691      89     611      93      74     190      62

Cuts at:   3665    4395     293 
   Size:        730    1519

  Fragments arranged by size: 

                1519     730     691     611     565     222     190     182
                 170     166      93      93      89      74      69      62
                  52      43
 
BspEI T'CCGG_A

Cuts at:   2166    4841    2166 
   Size:       2675    2946
 
BspHI T'CATG_A

Cuts at:    837    4797     837 
   Size:       3960    1661
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2671    2885    3187    3568    2671 
   Size:        214     302     381    4724

  Fragments arranged by size: 

                4724     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1340    3913    3967    5451    5616      50 
   Size:       1290    2573      54    1484     165      55

  Fragments arranged by size: 

                2573    1484    1290     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3533    3533 
   Size:       5621
 
BsrFI r'CCGG_y

Cuts at:   1304    2499    3619    3800    4009    1304 
   Size:       1195    1120     181     209    2916

  Fragments arranged by size: 

                2916    1195    1120     209     181
 
BssSI C'ACGA_G

Cuts at:   1360    3892    4250    1360 
   Size:       2532     358    2731

  Fragments arranged by size: 

                2731    2532     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2512    2998    2512 
   Size:        486    5135
 
BstBI TT'CG_AA

Cuts at:    110    3982     110 
   Size:       3872    1749
 
BstEII G'GTnAC_C

Cuts at:   2270    2270 
   Size:       5621
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1984    1984 
   Size:       5621
 
BstYI r'GATC_y

Cuts at:      1     470    2646    3113    3136    3471    3717    4729    4815 
   Size:        469    2176     467      23     335     246    1012      86

Cuts at:   4815    4827    5173       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2176    1012     469     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:   1070    1634    1817    2040    2397    2448    1070 
   Size:        564     183     223     357      51    4243

  Fragments arranged by size: 

                4243     564     357     223     183      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1754    3571    3752    3915    1754 
   Size:       1817     181     163    3460

  Fragments arranged by size: 

                3460    1817     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1763    2864    1763 
   Size:       1101    4520
 
DraI TTT'AAA

Cuts at:   1941    2620    1941 
   Size:        679    4942
 
DrdI GACnn_nn'nnGTC

Cuts at:    565    3327    4185     565 
   Size:       2762     858    2001

  Fragments arranged by size: 

                2762    2001     858
 
EaeI y'GGCC_r

Cuts at:   1306    1319    1717    2501    3206    3380    3771    3798    5143 
   Size:         13     398     784     705     174     391      27    1345

Cuts at:   5143    1306 
   Size:       1784

  Fragments arranged by size: 

                1784    1345     784     705     398     391     174      27
                  13
 
EagI C'GGCC_G

Cuts at:   1306    2501    3206    1306 
   Size:       1195     705    3721

  Fragments arranged by size: 

                3721    1195     705
 
EarI CTCTTCn'nnn_

Cuts at:    766    3644    3854     766 
   Size:       2878     210    2533

  Fragments arranged by size: 

                2878    2533     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3829    4139    4285    5431    3829 
   Size:        310     146    1146    4019

  Fragments arranged by size: 

                4019    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    291    2633     291 
   Size:       2342    3279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    418    1135    1240    2537    3446    3878    4625    4893     418 
   Size:        717     105    1297     909     432     747     268    1146

  Fragments arranged by size: 

                1297    1146     909     747     717     432     268     105
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1108    1108 
   Size:       5621
 
EcoO109I rG'GnC_Cy

Cuts at:    456     498     747     769    1687    1731    2542    2543    2760 
   Size:         42     249      22     918      44     811       1     217

Cuts at:   2760     456 
   Size:       3317

  Fragments arranged by size: 

                3317     918     811     249     217      44      42      22
                   1
 
EcoRI G'AATT_C

Cuts at:    129    2625     129 
   Size:       2496    3125
 
EcoRV GAT'ATC

Cuts at:    984    1969    2530     984 
   Size:        985     561    4075

  Fragments arranged by size: 

                4075     985     561
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    897    1248    3640     897 
   Size:        351    2392    2878

  Fragments arranged by size: 

                2878    2392     351
 
FseI GG_CCGG'CC

Cuts at:   1308    1308 
   Size:       5621
 
FspI TGC'GCA

Cuts at:   3402    3402 
   Size:       5621
 
HaeII r_GCGC'y

Cuts at:   1348    1814    1831    3055    3303    4325    5304    1348 
   Size:        466      17    1224     248    1022     979    1665

  Fragments arranged by size: 

                1665    1224    1022     979     466     248      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    434     466    3463    3493    3495    3525     434 
   Size:         32    2997      30       2      30    2530

  Fragments arranged by size: 

                2997    2530      32      30      30       2
 
HincII GTy'rAC

Cuts at:    570    2235    2660     570 
   Size:       1665     425    3531

  Fragments arranged by size: 

                3531    1665     425
 
HindIII A'AGCT_T

Cuts at:    868    1450    2492     868 
   Size:        582    1042    3997

  Fragments arranged by size: 

                3997    1042     582
 
KasI G'GCGC_C

Cuts at:   1344    3051    3299    1344 
   Size:       1707     248    3666

  Fragments arranged by size: 

                3666    1707     248
 
KpnI G_GTAC'C

Cuts at:   2641    2641 
   Size:       5621
 
MluI A'CGCG_T

Cuts at:   5054    5054 
   Size:       5621
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1949    4292    4476    1949 
   Size:       2343     184    3094

  Fragments arranged by size: 

                3094    2343     184
 
MscI TGG'CCA

Cuts at:   1321    3382    1321 
   Size:       2061    3560
 
MslI CAynn'nnrTG

Cuts at:    721    3610    3737     721 
   Size:       2889     127    2605

  Fragments arranged by size: 

                2889    2605     127
 
MspA1I CmG'CkG

Cuts at:   1317    1325    1528    1808    2732    3046    3406    4419    4664 
   Size:          8     203     280     924     314     360    1013     245

Cuts at:   4664    1317 
   Size:       2274

  Fragments arranged by size: 

                2274    1013     924     360     314     280     245     203
                   8
 
NaeI GCC'GGC

Cuts at:   1306    3802    1306 
   Size:       2496    3125
 
NarI GG'CG_CC

Cuts at:   1345    3052    3300    1345 
   Size:       1707     248    3666

  Fragments arranged by size: 

                3666    1707     248
 
NcoI C'CATG_G

Cuts at:   1070    1634    2448    1070 
   Size:        564     814    4243

  Fragments arranged by size: 

                4243     814     564
 
NgoMIV G'CCGG_C

Cuts at:   1304    3800    1304 
   Size:       2496    3125
 
NheI G'CTAG_C

Cuts at:   2001    2392    2001 
   Size:        391    5230
 
NsiI A_TGCA'T

Cuts at:    788     788 
   Size:       5621
 
NspI r_CATG'y

Cuts at:    781    1864    2674    3174    4081     781 
   Size:       1083     810     500     907    2321

  Fragments arranged by size: 

                2321    1083     907     810     500
 
PasI CC'CwG_GG

Cuts at:   2104    2104 
   Size:       5621
 
PciI A'CATG_T

Cuts at:   4077    4077 
   Size:       5621
 
PflMI CCAn_nnn'nTGG

Cuts at:   1201    2281    1201 
   Size:       1080    4541
 
PfoI T'CCnGG_A

Cuts at:   2125    2125 
   Size:       5621
 
PmeI GTTT'AAAC

Cuts at:   2620    2620 
   Size:       5621
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    992    1024    4760    4792     992 
   Size:         32    3736      32    1821

  Fragments arranged by size: 

                3736    1821      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    498    1687     498 
   Size:       1189    4432
 
PsiI TTA'TAA

Cuts at:    662     662 
   Size:       5621
 
PspOMI G'GGCC_C

Cuts at:    455     746    2542     455 
   Size:        291    1796    3534

  Fragments arranged by size: 

                3534    1796     291
 
PstI C_TGCA'G

Cuts at:   2463    2668    3353    2463 
   Size:        205     685    4731

  Fragments arranged by size: 

                4731     685     205
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5621
 
PvuII CAG'CTG

Cuts at:   1528    3046    3406    1528 
   Size:       1518     360    3743

  Fragments arranged by size: 

                3743    1518     360
 
RsrII CG'GwC_CG

Cuts at:   3816    3816 
   Size:       5621
 
SacI G_AGCT'C

Cuts at:    293    2635     293 
   Size:       2342    3279
 
SalI G'TCGA_C

Cuts at:   2658    2658 
   Size:       5621
 
SapI GCTCTTCn'nnn_

Cuts at:   3644    3854    3644 
   Size:        210    5411
 
SbfI CC_TGCA'GG

Cuts at:   2668    2668 
   Size:       5621
 
ScaI AGT'ACT

Cuts at:   5138    5138 
   Size:       5621
 
SexAI A'CCwGG_T

Cuts at:   1200    1200 
   Size:       5621
 
SfcI C'TryA_G

Cuts at:     33    1610    2019    2459    2468    2565    2664    3349    4342 
   Size:       1577     409     440       9      97      99     685     993

Cuts at:   4342    4533      33 
   Size:        191    1121

  Fragments arranged by size: 

                1577    1121     993     685     440     409     191      99
                  97       9
 
SfoI GGC'GCC

Cuts at:   1346    3053    3301    1346 
   Size:       1707     248    3666

  Fragments arranged by size: 

                3666    1707     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5621
 
SmaI CCC'GGG

Cuts at:   2643    2643 
   Size:       5621
 
SmlI C'TyrA_G

Cuts at:    466     819     871     890    1278    2776    3691    4183    4445 
   Size:        353      52      19     388    1498     915     492     262

Cuts at:   4445     466 
   Size:       1642

  Fragments arranged by size: 

                1642    1498     915     492     388     353     262      52
                  19
 
SnaBI TAC'GTA

Cuts at:   2613    2613 
   Size:       5621
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5621
 
SphI G_CATG'C

Cuts at:   1864    2674    3174    1864 
   Size:        810     500    4311

  Fragments arranged by size: 

                4311     810     500
 
StyI C'CwwG_G

Cuts at:    223     801    1070    1232    1634    2448    2755    3109     223 
   Size:        578     269     162     402     814     307     354    2735

  Fragments arranged by size: 

                2735     814     578     402     354     307     269     162
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3204    3337    3796    4005    3204 
   Size:        133     459     209    4820

  Fragments arranged by size: 

                4820     459     209     133
 
TatI w'GTAC_w

Cuts at:   1944    2199    5136    1944 
   Size:        255    2937    2429

  Fragments arranged by size: 

                2937    2429     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1351    1383    5138    5170    1351 
   Size:         32    3755      32    1802

  Fragments arranged by size: 

                3755    1802      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3418    3418 
   Size:       5621
 
XbaI T'CTAG_A

Cuts at:   2652    2652 
   Size:       5621
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1632    3116    1632 
   Size:       1484    4137
 
XmaI C'CCGG_G

Cuts at:   2641    2641 
   Size:       5621
 
XmnI GAAnn'nnTTC

Cuts at:    401    1007    1091     401 
   Size:        606      84    4931

  Fragments arranged by size: 

                4931     606      84
 
ZraI GAC'GTC

Cuts at:   1602    1602 
   Size:       5621
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI     PsiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AhdI     AleI     AlfI     AscI    AvrII     BaeI
     BbsI    BmgBI    BsiWI     BsmI    BsrGI   BssHII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    FspAI     HpaI     MfeI     NdeI     NotI
     NruI     PacI     PmlI    PshAI    PspXI     PsrI    SacII    SanDI
     SfiI    SgrAI     SrfI     SspI     StuI     SwaI     XhoI
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