Restriction map for pF1KSDA0019
 (Linear) (Six-base) MAPSORT of: KIAA0019.seq  Check: 9091  from: 1  to: 2484



KIAA0019.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AatII G_ACGT'C

Cuts at:      0    1478    2484 
   Size:       1478    1006
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    1114    2484 
   Size:       1009     105    1370

  Fragments arranged by size: 

                1370    1009     105
 
AflII C'TTAA_G

Cuts at:      0     745    2484 
   Size:        745    1739
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     866     898    2484 
   Size:        866      32    1586

  Fragments arranged by size: 

                1586     866      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1075    1564    2341    2484 
   Size:       1075     489     777     143

  Fragments arranged by size: 

                1075     777     489     143
 
ApaI G_GGCC'C

Cuts at:      0     333     624    2420    2484 
   Size:        333     291    1796      64

  Fragments arranged by size: 

                1796     333     291      64
 
ApoI r'AATT_y

Cuts at:      0       3     287     730     961    2484 
   Size:          3     284     443     231    1523

  Fragments arranged by size: 

                1523     443     284     231       3
 
AvaI C'yCGr_G

Cuts at:      0    1273    1755    1868    2258    2484 
   Size:       1273     482     113     390     226

  Fragments arranged by size: 

                1273     482     390     226     113
 
BanI G'GyrC_C

Cuts at:      0    1218    2484 
   Size:       1218    1266
 
BanII G_rGCy'C

Cuts at:      0     167     333     624    1189    1636    1729    2420    2484 
   Size:        167     166     291     565     447      93     691      64

  Fragments arranged by size: 

                 691     565     447     291     167     166      93      64
            
 
BbeI G_GCGC'C

Cuts at:      0    1222    2484 
   Size:       1222    1262
 
BbvCI CC'TCA_GC

Cuts at:      0    1616    2484 
   Size:       1616     868
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     592     626    2484 
   Size:        592      34    1858

  Fragments arranged by size: 

                1858     592      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1806    2484 
   Size:       1806     678
 
BclI T'GATC_A

Cuts at:      0    2165    2484 
   Size:       2165     319
 
BfrBI ATG'CAT

Cuts at:      0     660    2484 
   Size:        660    1824
 
BglI GCCn_nnn'nGGC

Cuts at:      0     619    1110    2484 
   Size:        619     491    1374

  Fragments arranged by size: 

                1374     619     491
 
BlpI GC'TnA_GC

Cuts at:      0    1769    2484 
   Size:       1769     715
 
Bme1580I G_kGCm'C

Cuts at:      0     333     624    1454    2420    2484 
   Size:        333     291     830     966      64

  Fragments arranged by size: 

                 966     830     333     291      64
 
BmtI G_CTAG'C

Cuts at:      0    1879    2270    2484 
   Size:       1879     391     214

  Fragments arranged by size: 

                1879     391     214
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    2411    2484 
   Size:        292    2119      73

  Fragments arranged by size: 

                2119     292      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    1616    2484 
   Size:       1616     868
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     714     749    1173    2484 
   Size:        361     353      35     424    1311

  Fragments arranged by size: 

                1311     424     361     353      35
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     967    1998    2484 
   Size:        967    1031     486

  Fragments arranged by size: 

                1031     967     486
 
BsaBI GATnn'nnATC

Cuts at:      0    1091    2484 
   Size:       1091    1393
 
BsaHI Gr'CG_yC

Cuts at:      0    1142    1219    1270    1475    2484 
   Size:       1142      77      51     205    1009

  Fragments arranged by size: 

                1142    1009     205      77      51
 
BsaWI w'CCGG_w

Cuts at:      0    2040    2484 
   Size:       2040     444
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     866     896    1225    1255    2484 
   Size:        866      30     329      30    1229

  Fragments arranged by size: 

                1229     866     329      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     175     577    1182    1324    1327    1711    2484 
   Size:        172       3     402     605     142       3     384     773

  Fragments arranged by size: 

                 773     605     402     384     172     142       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1750    1850    2119    2484 
   Size:       1750     100     269     365

  Fragments arranged by size: 

                1750     365     269     100
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104    2484 
   Size:        104    2380
 
BsiEI CG_ry'CG

Cuts at:      0    1183    2378    2484 
   Size:       1183    1195     106

  Fragments arranged by size: 

                1195    1183     106
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     402    1241    1284    2484 
   Size:        167     235     839      43    1200

  Fragments arranged by size: 

                1200     839     235     167      43
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1482    2230    2484 
   Size:       1482     748     254

  Fragments arranged by size: 

                1482     748     254
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     333     402     624    1189    1241    1284    1454 
   Size:        167     166      69     222     565      52      43     170

Cuts at:   1454    1636    1729    2420    2484 
   Size:        182      93     691      64

  Fragments arranged by size: 

                 691     565     222     182     170     167     166      93
                  69      64      52      43
 
BspEI T'CCGG_A

Cuts at:      0    2040    2484 
   Size:       2040     444
 
BspHI T'CATG_A

Cuts at:      0     711    2484 
   Size:        711    1773
 
BsrBI CCG'CTC

Cuts at:      0    1214    2484 
   Size:       1214    1270
 
BsrFI r'CCGG_y

Cuts at:      0    1178    2373    2484 
   Size:       1178    1195     111

  Fragments arranged by size: 

                1195    1178     111
 
BssSI C'ACGA_G

Cuts at:      0    1234    2484 
   Size:       1234    1250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2386    2484 
   Size:       2386      98
 
BstEII G'GTnAC_C

Cuts at:      0    2144    2484 
   Size:       2144     340
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1858    2484 
   Size:       1858     626
 
BstYI r'GATC_y

Cuts at:      0     344    2484 
   Size:        344    2140
 
BtgI C'CryG_G

Cuts at:      0     944    1508    1691    1914    2271    2322    2484 
   Size:        944     564     183     223     357      51     162

  Fragments arranged by size: 

                 944     564     357     223     183     162      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1628    2484 
   Size:       1628     856
 
BtsI GCAGTG_nn'

Cuts at:      0    1637    2484 
   Size:       1637     847
 
DraI TTT'AAA

Cuts at:      0    1815    2484 
   Size:       1815     669
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     439    2484 
   Size:        439    2045
 
EaeI y'GGCC_r

Cuts at:      0    1180    1193    1591    2375    2484 
   Size:       1180      13     398     784     109

  Fragments arranged by size: 

                1180     784     398     109      13
 
EagI C'GGCC_G

Cuts at:      0    1180    2375    2484 
   Size:       1180    1195     109

  Fragments arranged by size: 

                1195    1180     109
 
EarI CTCTTCn'nnn_

Cuts at:      0     640    2484 
   Size:        640    1844
 
EcoICRI GAG'CTC

Cuts at:      0     165    2484 
   Size:        165    2319
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    1009    1114    2411    2484 
   Size:        292     717     105    1297      73

  Fragments arranged by size: 

                1297     717     292     105      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     982    2484 
   Size:        982    1502
 
EcoO109I rG'GnC_Cy

Cuts at:      0     330     372     621     643    1561    1605    2416    2417 
   Size:        330      42     249      22     918      44     811       1

Cuts at:   2417    2484 
   Size:         67

  Fragments arranged by size: 

                 918     811     330     249      67      44      42      22
                   1
 
EcoRI G'AATT_C

Cuts at:      0       3    2484 
   Size:          3    2481
 
EcoRV GAT'ATC

Cuts at:      0     858    1843    2404    2484 
   Size:        858     985     561      80

  Fragments arranged by size: 

                 985     858     561      80
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     771    1122    2484 
   Size:        771     351    1362

  Fragments arranged by size: 

                1362     771     351
 
FseI GG_CCGG'CC

Cuts at:      0    1182    2484 
   Size:       1182    1302
 
HaeII r_GCGC'y

Cuts at:      0    1222    1688    1705    2484 
   Size:       1222     466      17     779

  Fragments arranged by size: 

                1222     779     466      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     308     340    2484 
   Size:        308      32    2144

  Fragments arranged by size: 

                2144     308      32
 
HincII GTy'rAC

Cuts at:      0     444    2109    2484 
   Size:        444    1665     375

  Fragments arranged by size: 

                1665     444     375
 
HindIII A'AGCT_T

Cuts at:      0     742    1324    2366    2484 
   Size:        742     582    1042     118

  Fragments arranged by size: 

                1042     742     582     118
 
KasI G'GCGC_C

Cuts at:      0    1218    2484 
   Size:       1218    1266
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1823    2484 
   Size:       1823     661
 
MscI TGG'CCA

Cuts at:      0    1195    2484 
   Size:       1195    1289
 
MslI CAynn'nnrTG

Cuts at:      0     595    2484 
   Size:        595    1889
 
MspA1I CmG'CkG

Cuts at:      0    1191    1199    1402    1682    2484 
   Size:       1191       8     203     280     802

  Fragments arranged by size: 

                1191     802     280     203       8
 
NaeI GCC'GGC

Cuts at:      0    1180    2484 
   Size:       1180    1304
 
NarI GG'CG_CC

Cuts at:      0    1219    2484 
   Size:       1219    1265
 
NcoI C'CATG_G

Cuts at:      0     944    1508    2322    2484 
   Size:        944     564     814     162

  Fragments arranged by size: 

                 944     814     564     162
 
NgoMIV G'CCGG_C

Cuts at:      0    1178    2484 
   Size:       1178    1306
 
NheI G'CTAG_C

Cuts at:      0    1875    2266    2484 
   Size:       1875     391     218

  Fragments arranged by size: 

                1875     391     218
 
NsiI A_TGCA'T

Cuts at:      0     662    2484 
   Size:        662    1822
 
NspI r_CATG'y

Cuts at:      0     655    1738    2484 
   Size:        655    1083     746

  Fragments arranged by size: 

                1083     746     655
 
PasI CC'CwG_GG

Cuts at:      0    1978    2484 
   Size:       1978     506
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1075    2155    2484 
   Size:       1075    1080     329

  Fragments arranged by size: 

                1080    1075     329
 
PfoI T'CCnGG_A

Cuts at:      0    1999    2484 
   Size:       1999     485
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     866     898    2484 
   Size:        866      32    1586

  Fragments arranged by size: 

                1586     866      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     372    1561    2484 
   Size:        372    1189     923

  Fragments arranged by size: 

                1189     923     372
 
PsiI TTA'TAA

Cuts at:      0     536    2484 
   Size:        536    1948
 
PspOMI G'GGCC_C

Cuts at:      0     329     620    2416    2484 
   Size:        329     291    1796      68

  Fragments arranged by size: 

                1796     329     291      68
 
PstI C_TGCA'G

Cuts at:      0    2337    2484 
   Size:       2337     147
 
PvuII CAG'CTG

Cuts at:      0    1402    2484 
   Size:       1402    1082
 
SacI G_AGCT'C

Cuts at:      0     167    2484 
   Size:        167    2317
 
SexAI A'CCwGG_T

Cuts at:      0    1074    2484 
   Size:       1074    1410
 
SfcI C'TryA_G

Cuts at:      0    1484    1893    2333    2342    2439    2484 
   Size:       1484     409     440       9      97      45

  Fragments arranged by size: 

                1484     440     409      97      45       9
 
SfoI GGC'GCC

Cuts at:      0    1220    2484 
   Size:       1220    1264
 
SmlI C'TyrA_G

Cuts at:      0     340     693     745     764    1152    2484 
   Size:        340     353      52      19     388    1332

  Fragments arranged by size: 

                1332     388     353     340      52      19
 
SphI G_CATG'C

Cuts at:      0    1738    2484 
   Size:       1738     746
 
StyI C'CwwG_G

Cuts at:      0      97     675     944    1106    1508    2322    2484 
   Size:         97     578     269     162     402     814     162

  Fragments arranged by size: 

                 814     578     402     269     162     162      97
 
TatI w'GTAC_w

Cuts at:      0    1818    2073    2484 
   Size:       1818     255     411

  Fragments arranged by size: 

                1818     411     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1225    1257    2484 
   Size:       1225      32    1227

  Fragments arranged by size: 

                1227    1225      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1506    2484 
   Size:       1506     978
 
XmnI GAAnn'nnTTC

Cuts at:      0     275     881     965    2484 
   Size:        275     606      84    1519

  Fragments arranged by size: 

                1519     606     275      84
 
ZraI GAC'GTC

Cuts at:      0    1476    2484 
   Size:       1476    1008
 

 Enzymes that do cut:

    AatII     AcuI    AflII     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    HaeII    Hin4I   HincII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NsiI     NspI     PasI    PflMI     PfoI     PpiI
    PpuMI     PsiI   PspOMI     PstI    PvuII     SacI    SexAI     SfcI
     SfoI     SmlI     SphI     StyI     TatI     TstI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BglII    BmgBI     BmrI     BplI    BsaAI    BsiWI
     BsmI    BspMI    BsrDI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII     EciI     FspI    FspAI     HpaI     KpnI
     MfeI     MluI     NdeI     NotI     NruI     PacI     PciI     PmeI
     PmlI    PshAI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com