Restriction map for pF1KSDA0007
 (Linear) (Six-base) MAPSORT of: KIAA0007.seq  Check: 1657  from: 1  to: 2031



KIAA0007.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0     599    2031 
   Size:        599    1432
 
Acc65I G'GTAC_C

Cuts at:      0     178    1632    2031 
   Size:        178    1454     399

  Fragments arranged by size: 

                1454     399     178
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1217    1832    1871    2031 
   Size:       1217     615      39     160

  Fragments arranged by size: 

                1217     615     160      39
 
AgeI A'CCGG_T

Cuts at:      0     728    2031 
   Size:        728    1303
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1032    2031 
   Size:       1032     999
 
AleI CACnn'nnGTG

Cuts at:      0     173    2031 
   Size:        173    1858
 
AlwNI CAG_nnn'CTG

Cuts at:      0     259     416    2031 
   Size:        259     157    1615

  Fragments arranged by size: 

                1615     259     157
 
ApaI G_GGCC'C

Cuts at:      0    1146    2031 
   Size:       1146     885
 
ApoI r'AATT_y

Cuts at:      0    1191    2031 
   Size:       1191     840
 
AseI AT'TA_AT

Cuts at:      0     358    2031 
   Size:        358    1673
 
BanI G'GyrC_C

Cuts at:      0     178     201    1212    1632    2031 
   Size:        178      23    1011     420     399

  Fragments arranged by size: 

                1011     420     399     178      23
 
BanII G_rGCy'C

Cuts at:      0    1146    2031 
   Size:       1146     885
 
BbeI G_GCGC'C

Cuts at:      0     205    2031 
   Size:        205    1826
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1370    1480    1756    1799    2031 
   Size:       1370     110     276      43     232

  Fragments arranged by size: 

                1370     276     232     110      43
 
BfrBI ATG'CAT

Cuts at:      0    1603    2031 
   Size:       1603     428
 
BglI GCCn_nnn'nGGC

Cuts at:      0     210    2031 
   Size:        210    1821
 
Bme1580I G_kGCm'C

Cuts at:      0    1146    2031 
   Size:       1146     885
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    1080    1354    2031 
   Size:        734     346     274     677

  Fragments arranged by size: 

                 734     677     346     274
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     580    2031 
   Size:        580    1451
 
BsaAI yAC'GTr

Cuts at:      0     156    2031 
   Size:        156    1875
 
BsaHI Gr'CG_yC

Cuts at:      0     202    2031 
   Size:        202    1829
 
BsaWI w'CCGG_w

Cuts at:      0     728    2031 
   Size:        728    1303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1244    1831    1888    1891    2031 
   Size:       1244     587      57       3     140

  Fragments arranged by size: 

                1244     587     140      57       3
 
BseYI C'CCAG_C

Cuts at:      0      43    1139    1637    1641    2031 
   Size:         43    1096     498       4     390

  Fragments arranged by size: 

                1096     498     390      43       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     168     797     927    2031 
   Size:        127      41     629     130    1104

  Fragments arranged by size: 

                1104     629     130     127      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     118    2031 
   Size:        118    1913
 
Bsp1286I G_dGCh'C

Cuts at:      0    1146    2031 
   Size:       1146     885
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     190     738    2031 
   Size:        190     548    1293

  Fragments arranged by size: 

                1293     548     190
 
BsrBI CCG'CTC

Cuts at:      0    1252    2031 
   Size:       1252     779
 
BsrDI GCAATG_nn'

Cuts at:      0    1337    2031 
   Size:       1337     694
 
BsrFI r'CCGG_y

Cuts at:      0     137     728    2031 
   Size:        137     591    1303

  Fragments arranged by size: 

                1303     591     137
 
BstYI r'GATC_y

Cuts at:      0    2004    2031 
   Size:       2004      27
 
BtsI GCAGTG_nn'

Cuts at:      0     513     785    2031 
   Size:        513     272    1246

  Fragments arranged by size: 

                1246     513     272
 
DraI TTT'AAA

Cuts at:      0    1091    2031 
   Size:       1091     940
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     382    2031 
   Size:        382    1649
 
EaeI y'GGCC_r

Cuts at:      0     128    2031 
   Size:        128    1903
 
EarI CTCTTCn'nnn_

Cuts at:      0     839    1796    1808    1904    2031 
   Size:        839     957      12      96     127

  Fragments arranged by size: 

                 957     839     127      96      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    1080    1217    1354    1832    1871    2031 
   Size:        734     346     137     137     478      39     160

  Fragments arranged by size: 

                 734     478     346     160     137     137      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     968    2031 
   Size:        968    1063
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48    1143    2031 
   Size:         48    1095     888

  Fragments arranged by size: 

                1095     888      48
 
EcoRI G'AATT_C

Cuts at:      0    1191    2031 
   Size:       1191     840
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     907    2031 
   Size:        907    1124
 
HaeII r_GCGC'y

Cuts at:      0     205    2031 
   Size:        205    1826
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1018    1050    1212    1244    2031 
   Size:       1018      32     162      32     787

  Fragments arranged by size: 

                1018     787     162      32      32
 
HincII GTy'rAC

Cuts at:      0     387     983    1592    2031 
   Size:        387     596     609     439

  Fragments arranged by size: 

                 609     596     439     387
 
HindIII A'AGCT_T

Cuts at:      0    1711    2031 
   Size:       1711     320
 
HpaI GTT'AAC

Cuts at:      0    1592    2031 
   Size:       1592     439
 
KasI G'GCGC_C

Cuts at:      0     201    2031 
   Size:        201    1830
 
KpnI G_GTAC'C

Cuts at:      0     182    1636    2031 
   Size:        182    1454     395

  Fragments arranged by size: 

                1454     395     182
 
MslI CAynn'nnrTG

Cuts at:      0     173    1703    2031 
   Size:        173    1530     328

  Fragments arranged by size: 

                1530     328     173
 
MspA1I CmG'CkG

Cuts at:      0     556    1641    2031 
   Size:        556    1085     390

  Fragments arranged by size: 

                1085     556     390
 
NarI GG'CG_CC

Cuts at:      0     202    2031 
   Size:        202    1829
 
NdeI CA'TA_TG

Cuts at:      0    1109    2031 
   Size:       1109     922
 
NsiI A_TGCA'T

Cuts at:      0    1605    2031 
   Size:       1605     426
 
NspI r_CATG'y

Cuts at:      0     473     622    2031 
   Size:        473     149    1409

  Fragments arranged by size: 

                1409     473     149
 
PasI CC'CwG_GG

Cuts at:      0    1401    2031 
   Size:       1401     630
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1662    2031 
   Size:       1662     369
 
PpuMI rG'GwC_Cy

Cuts at:      0      48    2031 
   Size:         48    1983
 
PsiI TTA'TAA

Cuts at:      0    1468    2031 
   Size:       1468     563
 
PspOMI G'GGCC_C

Cuts at:      0    1142    2031 
   Size:       1142     889
 
PstI C_TGCA'G

Cuts at:      0     218    2031 
   Size:        218    1813
 
PvuII CAG'CTG

Cuts at:      0     556    1641    2031 
   Size:        556    1085     390

  Fragments arranged by size: 

                1085     556     390
 
SanDI GG'GwC_CC

Cuts at:      0      48    2031 
   Size:         48    1983
 
SapI GCTCTTCn'nnn_

Cuts at:      0     839    2031 
   Size:        839    1192
 
ScaI AGT'ACT

Cuts at:      0    1442    2031 
   Size:       1442     589
 
SexAI A'CCwGG_T

Cuts at:      0    1628    2031 
   Size:       1628     403
 
SfcI C'TryA_G

Cuts at:      0     214     260     600    2031 
   Size:        214      46     340    1431

  Fragments arranged by size: 

                1431     340     214      46
 
SfoI GGC'GCC

Cuts at:      0     203    2031 
   Size:        203    1828
 
StuI AGG'CCT

Cuts at:      0      79    2031 
   Size:         79    1952
 
StyI C'CwwG_G

Cuts at:      0     221    2031 
   Size:        221    1810
 
TatI w'GTAC_w

Cuts at:      0     720    1440    2031 
   Size:        720     720     591

  Fragments arranged by size: 

                 720     720     591
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     682    2031 
   Size:        682    1349
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI     AhdI     AleI    AlwNI     ApaI
     ApoI     AseI     BanI    BanII     BbeI     BbsI    BfrBI     BglI
 Bme1580I     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstYI
     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MslI   MspA1I     NarI     NdeI     NsiI     NspI     PasI
    PflMI    PpuMI     PsiI   PspOMI     PstI    PvuII    SanDI     SapI
     ScaI    SexAI     SfcI     SfoI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AlfI     AloI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI
     MfeI     MluI     MmeI     MscI     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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