Restriction map for pF1KB8019
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AlwNI       AocI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BsgI        
BsiWI       BsmI        BspDI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        DraI        DrdI        
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FspI        HpaI        KpnI        
KspI        MaeI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII        647  TCCGGA               T^CCGGA
AcsI          136  AAATTC               [GA]^AATT[CT]
Alw44I        124  GTGCAC               G^TGCAC
ApaLI         124  GTGCAC               G^TGCAC
ApoI          136  AAATTC               [GA]^AATT[CT]
BbeI         1143  GGCGCC               GGCGC^C
BbrPI        1294  CACGTG               CAC^GTG
BbsI         1411  GTCTTC               GAAGAC(2/6)
BglII         185  AGATCT               A^GATCT
BpuAI        1411  GTCTTC               GAAGAC(2/6)
BsaAI        1294  CACGTG               [CT]AC^GT[GA]
BsaBI        1239  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         647  TCCGGA               [AT]^CCGG[AT]
BseAI         647  TCCGGA               T^CCGGA
Bsp1407I     1286  TGTACA               T^GTACA
BspEI         647  TCCGGA               T^CCGGA
BspHI         848  TCATGA               T^CATGA
BspLU11I      896  ACATGT               A^CATGT
BsrDI         938  GCAATG               GCAATG(2/0)
BsrGI        1286  TGTACA               T^GTACA
BssSI         597  CACGAG               CACGAG(-5/-1)
CpoI          692  CGGACCG              CG^G[AT]CCG
DraIII       1748  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FseI         1785  GGCCGGCC             GGCCGG^CC
HincII       1375  GTCAAC               GT[CT]^[GA]AC
HindII       1375  GTCAAC               GT[CT]^[GA]AC
HindIII       205  AAGCTT               A^AGCTT
KasI         1143  GGCGCC               G^GCGCC
MamI         1239  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          647  TCCGGA               T^CCGGA
MseI          146  TTAA                 T^TAA
NarI         1143  GGCGCC               GG^CGCC
PflMI        1007  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1294  CACGTG               CAC^GTG
PmlI         1294  CACGTG               CAC^GTG
RcaI          848  TCATGA               T^CATGA
RsrII         692  CGGACCG              CG^G[AT]CCG
SexAI          50  ACCTGGT              A^CC[AT]GGT
SfoI         1143  GGCGCC               GGC^GCC
SspBI        1286  TGTACA               T^GTACA
TfiI         1732  GAATC                G^A[AT]TC
Tru9I         146  TTAA                 T^TAA
Van91I       1007  CCAAGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        896  ACATGT               A^C[GA][CT]GT
             1293  ACACGT              
AhdI          526  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1904  GACCCCCTGTC         
ApaI          985  GGGCCC               GGGCC^C
             1676  GGGCCC              
AspEI         526  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1904  GACCCCCTGTC         
BalI          371  TGGCCA               TGG^CCA
             1004  TGGCCA              
BbvCI         543  CCTCAGC              CCTCAGC(-5/-2)
              250  GCTGAGG             
BpmI         1461  CTCCAG               CTGGAG(16/14)
             1210  CTGGAG              
BsmBI          40  GAGACG               CGTCTC(1/5)
              821  GAGACG              
BspMI         302  ACCTGC               ACCTGC(4/8)
              906  GCAGGT              
BsrBI         319  GAGCGG               CCGCTC(-3/-3)
             1312  GAGCGG              
Eam1105I      526  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1904  GACCCCCTGTC         
EarI         1138  GAAGAG               CTCTTC(1/4)
             1158  GAAGAG              
EcoNI         246  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              681  CCTTCGGCAGG         
Ksp632I      1138  GAAGAG               CTCTTC(1/4)
             1158  GAAGAG              
MluNI         371  TGGCCA               TGG^CCA
             1004  TGGCCA              
MscI          371  TGGCCA               TGG^CCA
             1004  TGGCCA              
NaeI          662  GCCGGC               GCC^GGC
             1786  GCCGGC              
NcoI          152  CCATGG               C^CATGG
              416  CCATGG              
NgoMIV        662  GCCGGC               G^CCGGC
             1786  GCCGGC              
NspI          896  ACATGT               [GA]CATG^[CT]
             1226  GCATGT              
PstI          904  CTGCAG               CTGCA^G
             1425  CTGCAG              
PvuII        1192  CAGCTG               CAG^CTG
             1767  CAGCTG              
SfcI          904  CTGCAG               C^T[GA][CT]AG
             1425  CTGCAG              
SgrAI         427  CACCGGCG             C[GA]^CCGG[CT]G
              661  CGCCGGCG            
StuI         1823  AGGCCT               AGG^CCT
             1871  AGGCCT              
TaqI          884  TCGA                 T^CGA
             1073  TCGA                
Tsp509I       137  AATT                 ^AATT
             1940  AATT                
TthHB8I       884  TCGA                 T^CGA
             1073  TCGA                
XcmI          563  CCAGCAGCTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1791  CCAGCTCAGCCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcgI        BcnI        BlpI        
BmyI        Bpu1102I    BsaHI       BsaI        BsaJI       
BseRI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmaI        
SmlI        StyI        TseI        Tsp45I      TspRI       
XhoII       XmaCI       XmaI        
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