Restriction map for pF1KB8005
 (Linear) (Six-base) MAPSORT of: KIBB8005.seq  Check: 8697  from: 1  to: 2244



KIBB8005.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     653    1861    1999    2244 
   Size:        391     262    1208     138     245

  Fragments arranged by size: 

                1208     391     262     245     138
 
AflIII A'CryG_T

Cuts at:      0     655    2224    2244 
   Size:        655    1569      20

  Fragments arranged by size: 

                1569     655      20
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1191    2090    2244 
   Size:       1191     899     154

  Fragments arranged by size: 

                1191     899     154
 
AlwNI CAG_nnn'CTG

Cuts at:      0     154    2244 
   Size:        154    2090
 
ApaI G_GGCC'C

Cuts at:      0      32    2244 
   Size:         32    2212
 
ApoI r'AATT_y

Cuts at:      0    1130    1273    2244 
   Size:       1130     143     971

  Fragments arranged by size: 

                1130     971     143
 
AseI AT'TA_AT

Cuts at:      0    1264    2244 
   Size:       1264     980
 
AvaI C'yCGr_G

Cuts at:      0    2036    2082    2244 
   Size:       2036      46     162

  Fragments arranged by size: 

                2036     162      46
 
BamHI G'GATC_C

Cuts at:      0    2029    2244 
   Size:       2029     215
 
BanII G_rGCy'C

Cuts at:      0      32    1871    2244 
   Size:         32    1839     373

  Fragments arranged by size: 

                1839     373      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     286     397     659    1849    2244 
   Size:        286     111     262    1190     395

  Fragments arranged by size: 

                1190     395     286     262     111
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1598    1720    2244 
   Size:       1598     122     524

  Fragments arranged by size: 

                1598     524     122
 
BfrBI ATG'CAT

Cuts at:      0    1663    2244 
   Size:       1663     581
 
BglII A'GATC_T

Cuts at:      0    1248    2244 
   Size:       1248     996
 
Bme1580I G_kGCm'C

Cuts at:      0      32     854    2244 
   Size:         32     822    1390

  Fragments arranged by size: 

                1390     822      32
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    1782    2244 
   Size:        734    1048     462

  Fragments arranged by size: 

                1048     734     462
 
Bpu10I CC'TnA_GC

Cuts at:      0     110    2244 
   Size:        110    2134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1630    2244 
   Size:       1630     614
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     410    1103    1233    2244 
   Size:        410     693     130    1011

  Fragments arranged by size: 

                1011     693     410     130
 
BsaBI GATnn'nnATC

Cuts at:      0    1580    2244 
   Size:       1580     664
 
BsaWI w'CCGG_w

Cuts at:      0    1428    1578    2244 
   Size:       1428     150     666

  Fragments arranged by size: 

                1428     666     150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     425     455    2244 
   Size:        425      30    1789

  Fragments arranged by size: 

                1789     425      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     705     737    2244 
   Size:        705      32    1507

  Fragments arranged by size: 

                1507     705      32
 
BseYI C'CCAG_C

Cuts at:      0     485    2244 
   Size:        485    1759
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2100    2244 
   Size:       2100     144
 
BsiEI CG_ry'CG

Cuts at:      0     571    2244 
   Size:        571    1673
 
BsiHKAI G_wGCw'C

Cuts at:      0     832    1871    2244 
   Size:        832    1039     373

  Fragments arranged by size: 

                1039     832     373
 
BsmI GAATG_Cn'

Cuts at:      0    1087    2244 
   Size:       1087    1157
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     832     854    1871    2244 
   Size:         32     800      22    1017     373

  Fragments arranged by size: 

                1017     800     373      32      22
 
BspEI T'CCGG_A

Cuts at:      0    1428    1578    2244 
   Size:       1428     150     666

  Fragments arranged by size: 

                1428     666     150
 
BspHI T'CATG_A

Cuts at:      0    1229    2244 
   Size:       1229    1015
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1159    2135    2244 
   Size:       1159     976     109

  Fragments arranged by size: 

                1159     976     109
 
BsrDI GCAATG_nn'

Cuts at:      0     142     556     892    1173    1396    1513    2062    2244 
   Size:        142     414     336     281     223     117     549     182

  Fragments arranged by size: 

                 549     414     336     281     223     182     142     117
            
 
BsrFI r'CCGG_y

Cuts at:      0     570    2244 
   Size:        570    1674
 
BsrGI T'GTAC_A

Cuts at:      0    1936    2244 
   Size:       1936     308
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     485    1456    2244 
   Size:        485     971     788

  Fragments arranged by size: 

                 971     788     485
 
BstYI r'GATC_y

Cuts at:      0     127     251     672     858     911     978    1248    1575 
   Size:        127     124     421     186      53      67     270     327

Cuts at:   1575    1581    2029    2244 
   Size:          6     448     215

  Fragments arranged by size: 

                 448     421     327     270     215     186     127     124
                  67      53       6
 
Bsu36I CC'TnA_GG

Cuts at:      0    1163    2244 
   Size:       1163    1081
 
DraI TTT'AAA

Cuts at:      0    1013    2244 
   Size:       1013    1231
 
EarI CTCTTCn'nnn_

Cuts at:      0     875    1193    1372    1988    2244 
   Size:        875     318     179     616     256

  Fragments arranged by size: 

                 875     616     318     256     179
 
EcoICRI GAG'CTC

Cuts at:      0    1869    2244 
   Size:       1869     375
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     653     734    1782    1861    1999    2244 
   Size:        391     262      81    1048      79     138     245

  Fragments arranged by size: 

                1048     391     262     245     138      81      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1166    2244 
   Size:       1166    1078
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1309    2244 
   Size:       1309     935
 
HaeII r_GCGC'y

Cuts at:      0     561    2244 
   Size:        561    1683
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     896     928    2244 
   Size:        896      32    1316

  Fragments arranged by size: 

                1316     896      32
 
HincII GTy'rAC

Cuts at:      0     948    2244 
   Size:        948    1296
 
MfeI C'AATT_G

Cuts at:      0     873    1027    1531    2244 
   Size:        873     154     504     713

  Fragments arranged by size: 

                 873     713     504     154
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     323    1437    2244 
   Size:        114     209    1114     807

  Fragments arranged by size: 

                1114     807     209     114
 
MslI CAynn'nnrTG

Cuts at:      0    1234    2244 
   Size:       1234    1010
 
NdeI CA'TA_TG

Cuts at:      0    1665    2244 
   Size:       1665     579
 
NsiI A_TGCA'T

Cuts at:      0    1665    2244 
   Size:       1665     579
 
NspI r_CATG'y

Cuts at:      0     659    2228    2244 
   Size:        659    1569      16

  Fragments arranged by size: 

                1569     659      16
 
PasI CC'CwG_GG

Cuts at:      0     854    2244 
   Size:        854    1390
 
PciI A'CATG_T

Cuts at:      0     655    2224    2244 
   Size:        655    1569      20

  Fragments arranged by size: 

                1569     655      20
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     637    1319    1360    2244 
   Size:        637     682      41     884

  Fragments arranged by size: 

                 884     682     637      41
 
PfoI T'CCnGG_A

Cuts at:      0     900    2244 
   Size:        900    1344
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1100    1132    2244 
   Size:       1100      32    1112

  Fragments arranged by size: 

                1112    1100      32
 
PshAI GACnn'nnGTC

Cuts at:      0     452    2244 
   Size:        452    1792
 
PsiI TTA'TAA

Cuts at:      0    1526    2244 
   Size:       1526     718
 
PspOMI G'GGCC_C

Cuts at:      0      28    2244 
   Size:         28    2216
 
PstI C_TGCA'G

Cuts at:      0     454    1422    1805    2051    2079    2244 
   Size:        454     968     383     246      28     165

  Fragments arranged by size: 

                 968     454     383     246     165      28
 
SacI G_AGCT'C

Cuts at:      0    1871    2244 
   Size:       1871     373
 
SapI GCTCTTCn'nnn_

Cuts at:      0     875    1988    2244 
   Size:        875    1113     256

  Fragments arranged by size: 

                1113     875     256
 
ScaI AGT'ACT

Cuts at:      0      53    2244 
   Size:         53    2191
 
SexAI A'CCwGG_T

Cuts at:      0      68    1112    2244 
   Size:         68    1044    1132

  Fragments arranged by size: 

                1132    1044      68
 
SfcI C'TryA_G

Cuts at:      0     146     450    1418    1801    2047    2075    2244 
   Size:        146     304     968     383     246      28     169

  Fragments arranged by size: 

                 968     383     304     246     169     146      28
 
SmlI C'TyrA_G

Cuts at:      0    1609    2036    2244 
   Size:       1609     427     208

  Fragments arranged by size: 

                1609     427     208
 
SspI AAT'ATT

Cuts at:      0     583    2244 
   Size:        583    1661
 
StuI AGG'CCT

Cuts at:      0    1292    2244 
   Size:       1292     952
 
StyI C'CwwG_G

Cuts at:      0    1096    1553    2244 
   Size:       1096     457     691

  Fragments arranged by size: 

                1096     691     457
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     434    2244 
   Size:        434    1810
 
TatI w'GTAC_w

Cuts at:      0      51    1936    2244 
   Size:         51    1885     308

  Fragments arranged by size: 

                1885     308      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     428     460    1463    1495    2222    2244 
   Size:        428      32    1003      32     727      22

  Fragments arranged by size: 

                1003     727     428      32      32      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1250    2244 
   Size:       1250     994
 
XhoI C'TCGA_G

Cuts at:      0    2036    2244 
   Size:       2036     208
 
XmnI GAAnn'nnTTC

Cuts at:      0    1676    1823    2244 
   Size:       1676     147     421

  Fragments arranged by size: 

                1676     421     147
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     ApaI     ApoI     AseI     AvaI
    BamHI    BanII     BbsI    BciVI    BfrBI    BglII Bme1580I     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BstXI    BstYI   Bsu36I     DraI     EarI
  EcoICRI  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII     MfeI
     MmeI     MslI     NdeI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PshAI     PsiI   PspOMI     PstI     SacI     SapI
     ScaI    SexAI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI
     BanI     BbeI    BbvCI     BcgI     BclI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI    BsaAI    BsaHI    BsiWI    BsmBI    BsrBI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PmeI     PmlI    PpuMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XmaI     ZraI
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