Restriction map for pF1KB8004
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BbrPI       
BbvCI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DrdI        Eam1105I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        MfeI        MluI        
MroI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SspBI       
SspI        StuI        SwaI        Tsp509I     Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AocI         1183  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1782  AGCGCT               AGC^GCT
ApaI         1612  GGGCCC               GGGCC^C
BalI            2  TGGCCA               TGG^CCA
BamHI        1121  GGATCC               G^GATCC
BbeI         1959  GGCGCC               GGCGC^C
BciVI         437  GTATCC               GTATCC(6/5)
BglII        1088  AGATCT               A^GATCT
BsaBI         387  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1692  GGTCTC               GGTCTC(1/5)
BsgI         1235  CTGCAC               GTGCAG(16/14)
BsiEI        1049  CGGCCG               CG[GA][CT]^CG
BsmBI        1044  CGTCTC               CGTCTC(1/5)
BsmI          267  GAATGC               GAATGC(1/-1)
BspHI         800  TCATGA               T^CATGA
BsrBI         612  CCGCTC               CCGCTC(-3/-3)
BsrDI         365  GCAATG               GCAATG(2/0)
BsrFI        2000  ACCGGC               [GA]^CCGG[CT]
BstXI        2019  CCACAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1183  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2000  ACCGGC               [GA]^CCGG[CT]
DraI          865  TTTAAA               TTT^AAA
EagI         1049  CGGCCG               C^GGCCG
EclXI        1049  CGGCCG               C^GGCCG
Eco47III     1782  AGCGCT               AGC^GCT
Eco52I       1049  CGGCCG               C^GGCCG
Eco81I       1183  CCTGAGG              CC^T[ACGT]AGG
KasI         1959  GGCGCC               G^GCGCC
KspI         1608  CCGCGG               CCGC^GG
MamI          387  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MseI          866  TTAA                 T^TAA
NarI         1959  GGCGCC               GG^CGCC
NcoI         1688  CCATGG               C^CATGG
PflMI        1633  CCACTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1359  CAGCTG               CAG^CTG
RcaI          800  TCATGA               T^CATGA
SacII        1608  CCGCGG               CCGC^GG
SbfI         1464  CCTGCAGG             CCTGCA^GG
ScaI         1565  AGTACT               AGT^ACT
SfoI         1959  GGCGCC               GGC^GCC
SphI          135  GCATGC               GCATG^C
Sse8387I     1464  CCTGCAGG             CCTGCA^GG
Tru9I         866  TTAA                 T^TAA
Van91I       1633  CCACTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I         61  GGTACC               G^GTACC
             1421  GGTACC              
AccI          520  GTCTAC               GT^[AC][GT]AC
              727  GTAGAC              
AccII        1609  CGCG                 CG^CG
             2095  CGCG                
Asp718I        61  GGTACC               G^GTACC
             1421  GGTACC              
BbsI          949  GAAGAC               GAAGAC(2/6)
             1409  GTCTTC              
BcgI          272  CGACCCCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              639  GCAGCACTATCG        
BglI         1951  GCCAAGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1968  GCCTGAACGGC         
BpuAI         949  GAAGAC               GAAGAC(2/6)
             1409  GTCTTC              
BsaWI        1289  TCCGGT               [AT]^CCGG[AT]
             1725  ACCGGA              
BstUI        1609  CGCG                 CG^CG
             2095  CGCG                
DraIII        238  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1634  CACTGAGTG           
EaeI            2  TGGCCA               [CT]^GGCC[GA]
             1049  CGGCCG              
EarI         1924  CTCTTC               CTCTTC(1/4)
              349  GAAGAG              
HindIII       294  AAGCTT               A^AGCTT
              631  AAGCTT              
KpnI           61  GGTACC               GGTAC^C
             1421  GGTACC              
Ksp632I      1924  CTCTTC               CTCTTC(1/4)
              349  GAAGAG              
MslI         1788  CACCCGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1914  CACGGCTGTG          
MvnI         1609  CGCG                 CG^CG
             2095  CGCG                
NspI          135  GCATGC               [GA]CATG^[CT]
             1485  GCATGT              
SacI         1675  GAGCTC               GAGCT^C
             1719  GAGCTC              
SapI          349  GAAGAGC              GCTCTTC(1/4)
             1923  GCTCTTC             
SmaI           26  CCCGGG               CCC^GGG
             1397  CCCGGG              
TaqI          896  TCGA                 T^CGA
             1526  TCGA                
TthHB8I       896  TCGA                 T^CGA
             1526  TCGA                
XmaCI          26  CCCGGG               C^CCGGG
             1397  CCCGGG              
XmaI           26  CCCGGG               C^CCGGG
             1397  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        TseI        Tsp45I      
TspRI       XcmI        XhoII       
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