Restriction map for pF1KB6008
##################################
# NO CUT ENZYMES (   44 enzymes) #
##################################

AflII       AgeI        AscI        AseI        AsnI        
AvrII       BclI        BfrI        BlnI        BsaBI       
BsiWI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     CpoI        DraI        EcoRI       FbaI        
FseI        HpaI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PinAI       PmeI        PshBI       
RsrII       SalI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SspI        SwaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AclI         6121  AACGTT               AA^CGTT
Aor51HI      1064  AGCGCT               AGC^GCT
Asp700I      5130  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3920  TGCGCA               TGC^GCA
BamHI        2717  GGATCC               G^GATCC
BciVI         163  GGATAC               GTATCC(6/5)
Bsp1407I      149  TGTACA               T^GTACA
BspLU11I     8152  ACATGT               A^CATGT
BsrGI         149  TGTACA               T^GTACA
DrdI          316  GACCAAAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1851  CGGCCG               C^GGCCG
EclXI        1851  CGGCCG               C^GGCCG
Eco47III     1064  AGCGCT               AGC^GCT
Eco52I       1851  CGGCCG               C^GGCCG
EcoRV        8236  GATATC               GAT^ATC
FspI         3920  TGCGCA               TGC^GCA
HindIII      2235  AAGCTT               A^AGCTT
NaeI         3608  GCCGGC               GCC^GGC
NgoMIV       3608  GCCGGC               G^CCGGC
PshAI        7423  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     6121  AACGTT               AA^CGTT
PvuI         2606  CGATCG               CGAT^CG
SapI          684  GAAGAGC              GCTCTTC(1/4)
SbfI         7082  CCTGCAGG             CCTGCA^GG
SphI         7745  GCATGC               GCATG^C
Sse8387I     7082  CCTGCAGG             CCTGCA^GG
SspBI         149  TGTACA               T^GTACA
XbaI          357  TCTAGA               T^CTAGA
XmnI         5130  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        298  GGTACC               G^GTACC
             4748  GGTACC              
AccI         4810  GTCTAC               GT^[AC][GT]AC
             6622  GTCTAC              
AccIII       1057  TCCGGA               T^CCGGA
             5011  TCCGGA              
AhdI         6240  GACGTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7525  GACTCCCAGTC         
AocI          994  CCTGAGG              CC^T[ACGT]AGG
             3620  CCTCAGG             
Asp718I       298  GGTACC               G^GTACC
             4748  GGTACC              
AspEI        6240  GACGTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7525  GACTCCCAGTC         
BbeI         1870  GGCGCC               GGCGC^C
             4797  GGCGCC              
BfaI          358  CTAG                 C^TAG
             2722  CTAG                
BglII        2141  AGATCT               A^GATCT
             6836  AGATCT              
BsaI         5812  GAGACC               GGTCTC(1/5)
             6084  GGTCTC              
BseAI        1057  TCCGGA               T^CCGGA
             5011  TCCGGA              
BspDI         418  ATCGAT               AT^CGAT
             7789  ATCGAT              
BspEI        1057  TCCGGA               T^CCGGA
             5011  TCCGGA              
BspHI        3149  TCATGA               T^CATGA
             7973  TCATGA              
BsrFI        2876  GCCGGT               [GA]^CCGG[CT]
             3608  GCCGGC              
Bsu36I        994  CCTGAGG              CC^T[ACGT]AGG
             3620  CCTCAGG             
Cfr10I       2876  GCCGGT               [GA]^CCGG[CT]
             3608  GCCGGC              
ClaI          418  ATCGAT               AT^CGAT
             7789  ATCGAT              
Eam1105I     6240  GACGTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7525  GACTCCCAGTC         
Eco81I        994  CCTGAGG              CC^T[ACGT]AGG
             3620  CCTCAGG             
EcoNI        2781  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4947  CCTCATCCAGG         
EcoT22I      5353  ATGCAT               ATGCA^T
             6139  ATGCAT              
KasI         1870  GGCGCC               G^GCGCC
             4797  GGCGCC              
KpnI          298  GGTACC               GGTAC^C
             4748  GGTACC              
MaeI          358  CTAG                 C^TAG
             2722  CTAG                
MroI         1057  TCCGGA               T^CCGGA
             5011  TCCGGA              
MseI          494  TTAA                 T^TAA
             5801  TTAA                
NarI         1870  GGCGCC               GG^CGCC
             4797  GGCGCC              
NsiI         5353  ATGCAT               ATGCA^T
             6139  ATGCAT              
PaeR7I       1091  CTCGAG               C^TCGAG
             2356  CTCGAG              
RcaI         3149  TCATGA               T^CATGA
             7973  TCATGA              
SacI         3081  GAGCTC               GAGCT^C
             6672  GAGCTC              
ScaI         4622  AGTACT               AGT^ACT
             8048  AGTACT              
SfoI         1870  GGCGCC               GGC^GCC
             4797  GGCGCC              
Tru9I         494  TTAA                 T^TAA
             5801  TTAA                
XhoI         1091  CTCGAG               C^TCGAG
             2356  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       ApaI        ApaLI       ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvCI       BbvI        
BcgI        BcnI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrI        BssKI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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