Restriction map for
pF1KB6002
(Linear) (Six-base) MAPSORT of: KIBB6002.seq Check: 9529 from: 1 to: 5058
KIBB6002.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 612 5058
Size: 612 4446
AccI GT'mk_AC
Cuts at: 0 4883 5058
Size: 4883 175
AclI AA'CG_TT
Cuts at: 0 2694 5058
Size: 2694 2364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 3318 3668 3683 4001 4638 4640 4778
Size: 1512 1806 350 15 318 637 2 138
Cuts at: 4778 5058
Size: 280
Fragments arranged by size:
1806 1512 637 350 318 280 138 15
2
AflIII A'CryG_T
Cuts at: 0 1318 2407 3084 3788 5058
Size: 1318 1089 677 704 1270
Fragments arranged by size:
1318 1270 1089 704 677
AleI CACnn'nnGTG
Cuts at: 0 3367 5058
Size: 3367 1691
ApoI r'AATT_y
Cuts at: 0 703 799 1180 1980 2084 2199 2297 2451
Size: 703 96 381 800 104 115 98 154
Cuts at: 2451 2845 2954 4340 4393 4838 4962 5058
Size: 394 109 1386 53 445 124 96
Fragments arranged by size:
1386 800 703 445 394 381 154 124
115 109 104 98 96 96 53
AseI AT'TA_AT
Cuts at: 0 3398 3896 5058
Size: 3398 498 1162
Fragments arranged by size:
3398 1162 498
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1585 1618 5058
Size: 1585 33 3440
Fragments arranged by size:
3440 1585 33
BamHI G'GATC_C
Cuts at: 0 819 903 3610 5058
Size: 819 84 2707 1448
Fragments arranged by size:
2707 1448 819 84
BanII G_rGCy'C
Cuts at: 0 373 1900 3861 4220 5058
Size: 373 1527 1961 359 838
Fragments arranged by size:
1961 1527 838 373 359
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 1180 1877 2716 4192 4368 5058
Size: 556 624 697 839 1476 176 690
Fragments arranged by size:
1476 839 697 690 624 556 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 1796 5058
Size: 1762 34 3262
Fragments arranged by size:
3262 1762 34
BclI T'GATC_A
Cuts at: 0 2853 5058
Size: 2853 2205
BfrBI ATG'CAT
Cuts at: 0 4737 5058
Size: 4737 321
BglII A'GATC_T
Cuts at: 0 2916 3423 3473 3495 4746 5058
Size: 2916 507 50 22 1251 312
Fragments arranged by size:
2916 1251 507 312 50 22
BlpI GC'TnA_GC
Cuts at: 0 4181 5058
Size: 4181 877
Bme1580I G_kGCm'C
Cuts at: 0 2010 5058
Size: 2010 3048
BmtI G_CTAG'C
Cuts at: 0 898 5058
Size: 898 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2041 4649 5058
Size: 2041 2608 409
Fragments arranged by size:
2608 2041 409
Bpu10I CC'TnA_GC
Cuts at: 0 3643 5058
Size: 3643 1415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 2540 3268 5028 5058
Size: 319 2221 728 1760 30
Fragments arranged by size:
2221 1760 728 319 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 1281 5058
Size: 1281 3777
BsaAI yAC'GTr
Cuts at: 0 1319 5058
Size: 1319 3739
BsaBI GATnn'nnATC
Cuts at: 0 218 1667 4738 5058
Size: 218 1449 3071 320
Fragments arranged by size:
3071 1449 320 218
BsaWI w'CCGG_w
Cuts at: 0 51 5058
Size: 51 5007
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4670 4700 5058
Size: 4670 30 358
Fragments arranged by size:
4670 358 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1150 1880 2543 3185 4694 5058
Size: 1150 730 663 642 1509 364
Fragments arranged by size:
1509 1150 730 663 642 364
BseYI C'CCAG_C
Cuts at: 0 1367 5058
Size: 1367 3691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4964 5058
Size: 4964 94
BsiHKAI G_wGCw'C
Cuts at: 0 3099 5058
Size: 3099 1959
BsmI GAATG_Cn'
Cuts at: 0 499 530 3378 4551 5058
Size: 499 31 2848 1173 507
Fragments arranged by size:
2848 1173 507 499 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3280 5011 5058
Size: 3280 1731 47
Fragments arranged by size:
3280 1731 47
Bsp1286I G_dGCh'C
Cuts at: 0 373 1900 2010 3099 3861 4220 5058
Size: 373 1527 110 1089 762 359 838
Fragments arranged by size:
1527 1089 838 762 373 359 110
BspEI T'CCGG_A
Cuts at: 0 51 5058
Size: 51 5007
BspHI T'CATG_A
Cuts at: 0 1467 5058
Size: 1467 3591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 612 4636 5058
Size: 612 4024 422
Fragments arranged by size:
4024 612 422
BsrDI GCAATG_nn'
Cuts at: 0 1452 1541 2785 4142 5058
Size: 1452 89 1244 1357 916
Fragments arranged by size:
1452 1357 1244 916 89
BsrGI T'GTAC_A
Cuts at: 0 2934 3060 3156 5058
Size: 2934 126 96 1902
Fragments arranged by size:
2934 1902 126 96
BstBI TT'CG_AA
Cuts at: 0 1023 3774 5058
Size: 1023 2751 1284
Fragments arranged by size:
2751 1284 1023
BstEII G'GTnAC_C
Cuts at: 0 4022 5058
Size: 4022 1036
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3000 5058
Size: 3000 2058
BstYI r'GATC_y
Cuts at: 0 819 903 2154 2866 2916 3423 3473 3495
Size: 819 84 1251 712 50 507 50 22
Cuts at: 3495 3610 4746 5058
Size: 115 1136 312
Fragments arranged by size:
1251 1136 819 712 507 312 115 84
50 50 22
BstZ17I GTA'TAC
Cuts at: 0 4884 5058
Size: 4884 174
Bsu36I CC'TnA_GG
Cuts at: 0 836 3577 5058
Size: 836 2741 1481
Fragments arranged by size:
2741 1481 836
BtgI C'CryG_G
Cuts at: 0 1580 5058
Size: 1580 3478
BtsI GCAGTG_nn'
Cuts at: 0 2319 5058
Size: 2319 2739
ClaI AT'CG_AT
Cuts at: 0 269 5058
Size: 269 4789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 56 91 3278 3313 5058
Size: 56 35 3187 35 1745
Fragments arranged by size:
3187 1745 56 35 35
DraI TTT'AAA
Cuts at: 0 30 710 1016 1589 2163 2303 2394 3618
Size: 30 680 306 573 574 140 91 1224
Cuts at: 3618 5058
Size: 1440
Fragments arranged by size:
1440 1224 680 574 573 306 140 91
30
DrdI GACnn_nn'nnGTC
Cuts at: 0 4262 5058
Size: 4262 796
EaeI y'GGCC_r
Cuts at: 0 424 5058
Size: 424 4634
EarI CTCTTCn'nnn_
Cuts at: 0 866 923 1112 1697 5058
Size: 866 57 189 585 3361
Fragments arranged by size:
3361 866 585 189 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 2041 3318 3668 3683 4001 4638 4640
Size: 1512 529 1277 350 15 318 637 2
Cuts at: 4640 4649 4778 5058
Size: 9 129 280
Fragments arranged by size:
1512 1277 637 529 350 318 280 129
15 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4664 5058
Size: 4664 394
EcoRV GAT'ATC
Cuts at: 0 11 267 792 2252 5058
Size: 11 256 525 1460 2806
Fragments arranged by size:
2806 1460 525 256 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 313 1028 2220 2638 4176 5058
Size: 313 715 1192 418 1538 882
Fragments arranged by size:
1538 1192 882 715 418 313
HaeII r_GCGC'y
Cuts at: 0 2747 5058
Size: 2747 2311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 1502 1534 5058
Size: 135 32 1335 32 3524
Fragments arranged by size:
3524 1335 135 32 32
HincII GTy'rAC
Cuts at: 0 2562 5058
Size: 2562 2496
HindIII A'AGCT_T
Cuts at: 0 630 2357 2675 2939 5058
Size: 630 1727 318 264 2119
Fragments arranged by size:
2119 1727 630 318 264
MfeI C'AATT_G
Cuts at: 0 2257 5058
Size: 2257 2801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4885 5058
Size: 4885 173
MscI TGG'CCA
Cuts at: 0 426 5058
Size: 426 4632
MslI CAynn'nnrTG
Cuts at: 0 1351 3367 4729 4732 5058
Size: 1351 2016 1362 3 326
Fragments arranged by size:
2016 1362 1351 326 3
MspA1I CmG'CkG
Cuts at: 0 23 5037 5058
Size: 23 5014 21
Fragments arranged by size:
5014 23 21
NdeI CA'TA_TG
Cuts at: 0 1249 3888 4782 5058
Size: 1249 2639 894 276
Fragments arranged by size:
2639 1249 894 276
NheI G'CTAG_C
Cuts at: 0 894 5058
Size: 894 4164
NsiI A_TGCA'T
Cuts at: 0 4739 5058
Size: 4739 319
NspI r_CATG'y
Cuts at: 0 2411 3088 3792 3826 5058
Size: 2411 677 704 34 1232
Fragments arranged by size:
2411 1232 704 677 34
PasI CC'CwG_GG
Cuts at: 0 1047 5058
Size: 1047 4011
PciI A'CATG_T
Cuts at: 0 2407 3084 3788 5058
Size: 2407 677 704 1270
Fragments arranged by size:
2407 1270 704 677
PfoI T'CCnGG_A
Cuts at: 0 567 2460 5058
Size: 567 1893 2598
Fragments arranged by size:
2598 1893 567
PsiI TTA'TAA
Cuts at: 0 203 1351 5058
Size: 203 1148 3707
Fragments arranged by size:
3707 1148 203
PstI C_TGCA'G
Cuts at: 0 1445 1950 4641 5058
Size: 1445 505 2691 417
Fragments arranged by size:
2691 1445 505 417
PvuII CAG'CTG
Cuts at: 0 5037 5058
Size: 5037 21
SapI GCTCTTCn'nnn_
Cuts at: 0 1697 5058
Size: 1697 3361
ScaI AGT'ACT
Cuts at: 0 660 5058
Size: 660 4398
SfcI C'TryA_G
Cuts at: 0 542 766 1337 1441 1752 1946 1997 2506
Size: 542 224 571 104 311 194 51 509
Cuts at: 2506 4085 4637 5058
Size: 1579 552 421
Fragments arranged by size:
1579 571 552 542 509 421 311 224
194 104 51
SmlI C'TyrA_G
Cuts at: 0 298 2555 3283 5007 5058
Size: 298 2257 728 1724 51
Fragments arranged by size:
2257 1724 728 298 51
SspI AAT'ATT
Cuts at: 0 1020 2735 3315 5058
Size: 1020 1715 580 1743
Fragments arranged by size:
1743 1715 1020 580
StuI AGG'CCT
Cuts at: 0 3955 5058
Size: 3955 1103
StyI C'CwwG_G
Cuts at: 0 907 1458 1828 5058
Size: 907 551 370 3230
Fragments arranged by size:
3230 907 551 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 2729 3082 4963 5058
Size: 301 2428 353 1881 95
Fragments arranged by size:
2428 1881 353 301 95
TatI w'GTAC_w
Cuts at: 0 658 694 1125 2934 3060 3156 3243 4582
Size: 658 36 431 1809 126 96 87 1339
Cuts at: 4582 4935 5058
Size: 353 123
Fragments arranged by size:
1809 1339 658 431 353 126 123 96
87 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4670 4702 4804 4836 5058
Size: 4670 32 102 32 222
Fragments arranged by size:
4670 222 102 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1981 2756 3595 3635 5058
Size: 1981 775 839 40 1423
Fragments arranged by size:
1981 1423 839 775 40
XmnI GAAnn'nnTTC
Cuts at: 0 1295 2203 2273 3134 3425 4966 4999 5058
Size: 1295 908 70 861 291 1541 33 59
Fragments arranged by size:
1541 1295 908 861 291 70 59 33
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI ApoI AseI
BaeI BamHI BanII BbsI BcgI BclI BfrBI BglII
BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRV
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NdeI NheI NsiI NspI PasI PciI
PfoI PsiI PstI PvuII SapI ScaI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BanI
BbeI BbvCI BciVI BglI BmgBI BmrI BplI BsaHI
BsiEI BsiWI BsrBI BsrFI BssHII BssSI BstAPI BtgZI
DraIII EagI EciI EcoICRI EcoO109I EcoRI FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NotI NruI PacI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XhoI XmaI ZraI