Restriction map for pF1KB5544
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AocI        AscI        Asp718I     AvrII       BbeI        
BbrPI       BciVI       BfrI        BglII       BlnI        
BsaAI       BsaBI       BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
HindIII     KasI        KpnI        MamI        MfeI        
MluI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        1440  GACGTC               GACGT^C
AcyI         1440  GACGTC               G[GA]^CG[CT]C
AhdI          322  GACAATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3172  GTGCAC               G^TGCAC
Aor51HI      2567  AGCGCT               AGC^GCT
ApaLI        3172  GTGCAC               G^TGCAC
AspEI         322  GACAATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1292  TGCGCA               TGC^GCA
BcgI         1538  GCACGCTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          714  CCGGG                CC^[GC]GG
BsaHI        1440  GACGTC               G[GA]^CG[CT]C
BsiEI        1619  CGGCCG               CG[GA][CT]^CG
BsmI         2601  GCATTC               GAATGC(1/-1)
BspMI        1089  GCAGGT               ACCTGC(4/8)
BstXI         459  CCACAGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          391  GACTATTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1619  CGGCCG               C^GGCCG
Eam1105I      322  GACAATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1619  CGGCCG               C^GGCCG
Eco47III     2567  AGCGCT               AGC^GCT
Eco52I       1619  CGGCCG               C^GGCCG
FspI         1292  TGCGCA               TGC^GCA
HaeII        2567  AGCGCT               [GA]GCGC^[CT]
Hin1I        1440  GACGTC               G[GA]^CG[CT]C
KspI         2106  CCGCGG               CCGC^GG
NciI          714  CCGGG                CC^[GC]GG
NdeI         2654  CATATG               CA^TATG
SacI         2515  GAGCTC               GAGCT^C
SacII        2106  CCGCGG               CCGC^GG
SapI         2940  GAAGAGC              GCTCTTC(1/4)
ScaI         3111  AGTACT               AGT^ACT
SfiI         1512  GGCCACAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         3224  GCATGC               GCATG^C
SspI         1341  AATATT               AAT^ATT
XcmI         1664  CCACAGAGGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII        809  TCCGGA               T^CCGGA
             2463  TCCGGA              
AcsI          445  GAATTT               [GA]^AATT[CT]
             3655  GAATTT              
ApoI          445  GAATTT               [GA]^AATT[CT]
             3655  GAATTT              
AseI         2719  ATTAAT               AT^TAAT
             3616  ATTAAT              
AsnI         2719  ATTAAT               AT^TAAT
             3616  ATTAAT              
BamHI         642  GGATCC               G^GATCC
              699  GGATCC              
BbvCI        1625  CCTCAGC              CCTCAGC(-5/-2)
             3134  CCTCAGC             
BglI         1402  GCCGACATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1513  GCCACAGTGGC         
BlpI          470  GCTCAGC              GC^T[ACGT]AGC
             4221  GCTTAGC             
Bpu1102I      470  GCTCAGC              GC^T[ACGT]AGC
             4221  GCTTAGC             
BsaI          688  GAGACC               GGTCTC(1/5)
              813  GAGACC              
BsaWI         809  TCCGGA               [AT]^CCGG[AT]
             2463  TCCGGA              
BseAI         809  TCCGGA               T^CCGGA
             2463  TCCGGA              
BsmBI        1385  CGTCTC               CGTCTC(1/5)
             1883  CGTCTC              
BspEI         809  TCCGGA               T^CCGGA
             2463  TCCGGA              
BsrBI        1774  GAGCGG               CCGCTC(-3/-3)
             1849  GAGCGG              
BsrFI        1549  GCCGGC               [GA]^CCGG[CT]
             1984  GCCGGC              
BssSI         115  CACGAG               CACGAG(-5/-1)
             1783  CACGAG              
BstAPI        927  GCAGCGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3672  GCATCCTCTGC         
CelII         470  GCTCAGC              GC^T[ACGT]AGC
             4221  GCTTAGC             
Cfr10I       1549  GCCGGC               [GA]^CCGG[CT]
             1984  GCCGGC              
DraIII       1593  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3471  CACACAGTG           
HgaI          930  GCGTC                GACGC(5/10)
             1384  GCGTC               
HpaI         3256  GTTAAC               GTT^AAC
             3406  GTTAAC              
MroI          809  TCCGGA               T^CCGGA
             2463  TCCGGA              
NaeI         1549  GCCGGC               GCC^GGC
             1984  GCCGGC              
NcoI          596  CCATGG               C^CATGG
             1472  CCATGG              
NgoMIV       1549  GCCGGC               G^CCGGC
             1984  GCCGGC              
PaeR7I       2423  CTCGAG               C^TCGAG
             3569  CTCGAG              
PpuMI         716  GGGACCT              [GA]G^G[AT]CC[CT]
             2775  AGGACCT             
PshBI        2719  ATTAAT               AT^TAAT
             3616  ATTAAT              
XhoI         2423  CTCGAG               C^TCGAG
             3569  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BclI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BsrDI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XhoII       XmnI        
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