Restriction map for pF1KB5544
 (Linear) (Six-base) MAPSORT of: KIBB5544.seq  Check: 515  from: 1  to: 4335



KIBB5544.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1080    4335 
   Size:       1080    3255
 
AatII G_ACGT'C

Cuts at:      0    1444    4335 
   Size:       1444    2891
 
AccI GT'mk_AC

Cuts at:      0     414    2788    4268    4335 
   Size:        414    2374    1480      67

  Fragments arranged by size: 

                2374    1480     414      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1382    2144    2959    3295    3395    3530    4335 
   Size:        146    1236     762     815     336     100     135     805

  Fragments arranged by size: 

                1236     815     805     762     336     146     135     100
            
 
AfeI AGC'GCT

Cuts at:      0    2569    4335 
   Size:       2569    1766
 
AhdI GACnn_n'nnGTC

Cuts at:      0     327    4335 
   Size:        327    4008
 
AleI CACnn'nnGTG

Cuts at:      0     777    2023    2510    3179    4335 
   Size:        777    1246     487     669    1156

  Fragments arranged by size: 

                1246    1156     777     669     487
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     154    3197    4335 
   Size:        154    3043    1138

  Fragments arranged by size: 

                3043    1138     154
 
AlwNI CAG_nnn'CTG

Cuts at:      0     303     933    1071    4110    4309    4335 
   Size:        303     630     138    3039     199      26

  Fragments arranged by size: 

                3039     630     303     199     138      26
 
ApaI G_GGCC'C

Cuts at:      0     111    1492    1691    3163    4335 
   Size:        111    1381     199    1472    1172

  Fragments arranged by size: 

                1472    1381    1172     199     111
 
ApaLI G'TGCA_C

Cuts at:      0    3172    4335 
   Size:       3172    1163
 
ApoI r'AATT_y

Cuts at:      0     445    3655    4335 
   Size:        445    3210     680

  Fragments arranged by size: 

                3210     680     445
 
AseI AT'TA_AT

Cuts at:      0    2720    3617    4335 
   Size:       2720     897     718

  Fragments arranged by size: 

                2720     897     718
 
AvaI C'yCGr_G

Cuts at:      0    1422    2423    3094    3569    4335 
   Size:       1422    1001     671     475     766

  Fragments arranged by size: 

                1422    1001     766     671     475
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     744     777    3362    3395    4335 
   Size:        744      33    2585      33     940

  Fragments arranged by size: 

                2585     940     744      33      33
 
BamHI G'GATC_C

Cuts at:      0     642     699    4335 
   Size:        642      57    3636

  Fragments arranged by size: 

                3636     642      57
 
BanI G'GyrC_C

Cuts at:      0     190    1726    3202    4335 
   Size:        190    1536    1476    1133

  Fragments arranged by size: 

                1536    1476    1133     190
 
BanII G_rGCy'C

Cuts at:      0     111     481    1492    1691    1868    2519    3163    4335 
   Size:        111     370    1011     199     177     651     644    1172

  Fragments arranged by size: 

                1172    1011     651     644     370     199     177     111
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     798    1192    1421    2132    2414    2877    4335 
   Size:        323     475     394     229     711     282     463    1458

  Fragments arranged by size: 

                1458     711     475     463     394     323     282     229
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1626    3135    4335 
   Size:       1626    1509    1200

  Fragments arranged by size: 

                1626    1509    1200
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1527    1561    4335 
   Size:       1527      34    2774

  Fragments arranged by size: 

                2774    1527      34
 
BclI T'GATC_A

Cuts at:      0    2658    2883    4154    4335 
   Size:       2658     225    1271     181

  Fragments arranged by size: 

                2658    1271     225     181
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1408    1519    4335 
   Size:       1408     111    2816

  Fragments arranged by size: 

                2816    1408     111
 
BlpI GC'TnA_GC

Cuts at:      0     471    4222    4335 
   Size:        471    3751     113

  Fragments arranged by size: 

                3751     471     113
 
Bme1580I G_kGCm'C

Cuts at:      0     111    1379    1492    1691    1729    2030    3163    3176 
   Size:        111    1268     113     199      38     301    1133      13

Cuts at:   3176    4089    4335 
   Size:        913     246

  Fragments arranged by size: 

                1268    1133     913     301     246     199     113     111
                  38      13
 
BmgBI CAC'GTC

Cuts at:      0     218    4335 
   Size:        218    4117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     186     219     462    1021    1490    4335 
   Size:        186      33     243     559     469    2845

  Fragments arranged by size: 

                2845     559     469     243     186      33
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1821    4335 
   Size:       1821    2514
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1119    1482    1667    2177    2304    2826    4132    4193 
   Size:       1119     363     185     510     127     522    1306      61

Cuts at:   4193    4335 
   Size:        142

  Fragments arranged by size: 

                1306    1119     522     510     363     185     142     127
                  61
 
Bpu10I CC'TnA_GC

Cuts at:      0     203    1626    3135    4335 
   Size:        203    1423    1509    1200

  Fragments arranged by size: 

                1509    1423    1200     203
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    4335 
   Size:        862    3473
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     682     807    4335 
   Size:        682     125    3528

  Fragments arranged by size: 

                3528     682     125
 
BsaHI Gr'CG_yC

Cuts at:      0    1441    4335 
   Size:       1441    2894
 
BsaWI w'CCGG_w

Cuts at:      0     809    2463    4335 
   Size:        809    1654    1872

  Fragments arranged by size: 

                1872    1654     809
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1132    1162    1174    1204    1371    1401    2277    2307 
   Size:       1132      30      12      30     167      30     876      30

Cuts at:   2307    4335 
   Size:       2028

  Fragments arranged by size: 

                2028    1132     876     167      30      30      30      30
                  12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1485    1569    1801    1861    2059    2372    3141    3331 
   Size:       1485      84     232      60     198     313     769     190

Cuts at:   3331    4335 
   Size:       1004

  Fragments arranged by size: 

                1485    1004     769     313     232     198     190      84
                  60
 
BseYI C'CCAG_C

Cuts at:      0    1588    1867    4103    4335 
   Size:       1588     279    2236     232

  Fragments arranged by size: 

                2236    1588     279     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     160     620    1084    3379    4335 
   Size:         97      63     460     464    2295     956

  Fragments arranged by size: 

                2295     956     464     460      97      63
 
BsiEI CG_ry'CG

Cuts at:      0    1622    4335 
   Size:       1622    2713
 
BsiHKAI G_wGCw'C

Cuts at:      0      62     209    1784    2276    2519    2947    3176    3510 
   Size:         62     147    1575     492     243     428     229     334

Cuts at:   3510    3570    4335 
   Size:         60     765

  Fragments arranged by size: 

                1575     765     492     428     334     243     229     147
                  62      60
 
BsmI GAATG_Cn'

Cuts at:      0    2601    4335 
   Size:       2601    1734
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1391    1889    4335 
   Size:       1391     498    2446

  Fragments arranged by size: 

                2446    1391     498
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     111     209     481    1379    1492    1691    1729 
   Size:         62      49      98     272     898     113     199      38

Cuts at:   1729    1784    1868    2030    2276    2519    2947    3163    3176 
   Size:         55      84     162     246     243     428     216      13

Cuts at:   3176    3510    3570    4089    4335 
   Size:        334      60     519     246

  Fragments arranged by size: 

                 898     519     428     334     272     246     246     243
                 216     199     162     113      98      84      62      60
                  55      49      38      13
 
BspEI T'CCGG_A

Cuts at:      0     809    2463    4335 
   Size:        809    1654    1872

  Fragments arranged by size: 

                1872    1654     809
 
BspHI T'CATG_A

Cuts at:      0     678    1133    4047    4335 
   Size:        678     455    2914     288

  Fragments arranged by size: 

                2914     678     455     288
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1080    4335 
   Size:       1080    3255
 
BsrBI CCG'CTC

Cuts at:      0    1776    1851    4335 
   Size:       1776      75    2484

  Fragments arranged by size: 

                2484    1776      75
 
BsrDI GCAATG_nn'

Cuts at:      0     471    2264    2762    3586    3657    4223    4335 
   Size:        471    1793     498     824      71     566     112

  Fragments arranged by size: 

                1793     824     566     498     471     112      71
 
BsrFI r'CCGG_y

Cuts at:      0    1549    1984    4335 
   Size:       1549     435    2351

  Fragments arranged by size: 

                2351    1549     435
 
BssSI C'ACGA_G

Cuts at:      0     115    1783    4335 
   Size:        115    1668    2552

  Fragments arranged by size: 

                2552    1668     115
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     933    3678    4335 
   Size:        933    2745     657

  Fragments arranged by size: 

                2745     933     657
 
BstEII G'GTnAC_C

Cuts at:      0    1530    1830    2010    4335 
   Size:       1530     300     180    2325

  Fragments arranged by size: 

                2325    1530     300     180
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     466    4335 
   Size:        466    3869
 
BstYI r'GATC_y

Cuts at:      0     143     642     699    3130    4335 
   Size:        143     499      57    2431    1205

  Fragments arranged by size: 

                2431    1205     499     143      57
 
BtgI C'CryG_G

Cuts at:      0     596     654     866    1472    2106    4335 
   Size:        596      58     212     606     634    2229

  Fragments arranged by size: 

                2229     634     606     596     212      58
 
BtsI GCAGTG_nn'

Cuts at:      0     143    1084    1943    2591    3174    3186    4335 
   Size:        143     941     859     648     583      12    1149

  Fragments arranged by size: 

                1149     941     859     648     583     143      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3473    3508    4335 
   Size:       3473      35     827

  Fragments arranged by size: 

                3473     827      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1598    3476    4335 
   Size:       1598    1878     859

  Fragments arranged by size: 

                1878    1598     859
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     397    4335 
   Size:        397    3938
 
EaeI y'GGCC_r

Cuts at:      0     599    1619    2893    3787    4335 
   Size:        599    1020    1274     894     548

  Fragments arranged by size: 

                1274    1020     894     599     548
 
EagI C'GGCC_G

Cuts at:      0    1619    4335 
   Size:       1619    2716
 
EarI CTCTTCn'nnn_

Cuts at:      0    1401    1816    2935    3549    4007    4335 
   Size:       1401     415    1119     614     458     328

  Fragments arranged by size: 

                1401    1119     614     458     415     328
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     948    1985    4335 
   Size:        948    1037    2350

  Fragments arranged by size: 

                2350    1037     948
 
EcoICRI GAG'CTC

Cuts at:      0    2517    4335 
   Size:       2517    1818
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1119    1382    1482    1667    2144    2177    2304 
   Size:        146     973     263     100     185     477      33     127

Cuts at:   2304    2826    2959    3295    3395    3530    4132    4193    4335 
   Size:        522     133     336     100     135     602      61     142

  Fragments arranged by size: 

                 973     602     522     477     336     263     185     146
                 142     135     133     127     100     100      61      33
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2192    2687    4023    4335 
   Size:       2192     495    1336     312

  Fragments arranged by size: 

                2192    1336     495     312
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     108     717    1489    1687    2776    3160    4335 
   Size:        107       1     609     772     198    1089     384    1175

  Fragments arranged by size: 

                1175    1089     772     609     384     198     107       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     708    3376    4237    4335 
   Size:        708    2668     861      98

  Fragments arranged by size: 

                2668     861     708      98
 
FspI TGC'GCA

Cuts at:      0    1294    4335 
   Size:       1294    3041
 
HaeII r_GCGC'y

Cuts at:      0    2571    4335 
   Size:       2571    1764
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     313     345    2202    2234    3045    3077    3702    3734 
   Size:        313      32    1857      32     811      32     625      32

Cuts at:   3734    3842    3874    4335 
   Size:        108      32     461

  Fragments arranged by size: 

                1857     811     625     461     313     108      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      89    3258    3408    4335 
   Size:         89    3169     150     927

  Fragments arranged by size: 

                3169     927     150      89
 
HpaI GTT'AAC

Cuts at:      0    3258    3408    4335 
   Size:       3258     150     927

  Fragments arranged by size: 

                3258     927     150
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1261    3329    4335 
   Size:       1261    2068    1006

  Fragments arranged by size: 

                2068    1261    1006
 
MscI TGG'CCA

Cuts at:      0     601    2895    3789    4335 
   Size:        601    2294     894     546

  Fragments arranged by size: 

                2294     894     601     546
 
MslI CAynn'nnrTG

Cuts at:      0     464     777    1054    1060    2023    2269    2399    2510 
   Size:        464     313     277       6     963     246     130     111

Cuts at:   2510    2842    3179    3401    3739    4335 
   Size:        332     337     222     338     596

  Fragments arranged by size: 

                 963     596     464     338     337     332     313     277
                 246     222     130     111       6
 
MspA1I CmG'CkG

Cuts at:      0     230     475    1157    2108    2155    3393    4335 
   Size:        230     245     682     951      47    1238     942

  Fragments arranged by size: 

                1238     951     942     682     245     230      47
 
NaeI GCC'GGC

Cuts at:      0    1551    1986    4335 
   Size:       1551     435    2349

  Fragments arranged by size: 

                2349    1551     435
 
NcoI C'CATG_G

Cuts at:      0     596    1472    4335 
   Size:        596     876    2863

  Fragments arranged by size: 

                2863     876     596
 
NdeI CA'TA_TG

Cuts at:      0    2655    4335 
   Size:       2655    1680
 
NgoMIV G'CCGG_C

Cuts at:      0    1549    1984    4335 
   Size:       1549     435    2351

  Fragments arranged by size: 

                2351    1549     435
 
NspI r_CATG'y

Cuts at:      0    1293    2959    3228    4335 
   Size:       1293    1666     269    1107

  Fragments arranged by size: 

                1666    1293    1107     269
 
PasI CC'CwG_GG

Cuts at:      0     735    4335 
   Size:        735    3600
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3592    3762    4110    4335 
   Size:       3592     170     348     225

  Fragments arranged by size: 

                3592     348     225     170
 
PfoI T'CCnGG_A

Cuts at:      0     713     973    1096    4335 
   Size:        713     260     123    3239

  Fragments arranged by size: 

                3239     713     260     123
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     325    3526    3558    4335 
   Size:        293      32    3201      32     777

  Fragments arranged by size: 

                3201     777     293      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     717    2776    4335 
   Size:        717    2059    1559

  Fragments arranged by size: 

                2059    1559     717
 
PsiI TTA'TAA

Cuts at:      0    3664    4335 
   Size:       3664     671
 
PspOMI G'GGCC_C

Cuts at:      0     107    1488    1687    3159    4335 
   Size:        107    1381     199    1472    1176

  Fragments arranged by size: 

                1472    1381    1176     199     107
 
PspXI vC'TCGA_Gb

Cuts at:      0    2423    3569    4335 
   Size:       2423    1146     766

  Fragments arranged by size: 

                2423    1146     766
 
PstI C_TGCA'G

Cuts at:      0    1091    2598    3181    4335 
   Size:       1091    1507     583    1154

  Fragments arranged by size: 

                1507    1154    1091     583
 
PvuII CAG'CTG

Cuts at:      0     230    1157    3393    4335 
   Size:        230     927    2236     942

  Fragments arranged by size: 

                2236     942     927     230
 
SacI G_AGCT'C

Cuts at:      0    2519    4335 
   Size:       2519    1816
 
SacII CC_GC'GG

Cuts at:      0    2109    4335 
   Size:       2109    2226
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2935    4335 
   Size:       2935    1400
 
ScaI AGT'ACT

Cuts at:      0    3113    4335 
   Size:       3113    1222
 
SfcI C'TryA_G

Cuts at:      0    1087    1152    2594    3177    4335 
   Size:       1087      65    1442     583    1158

  Fragments arranged by size: 

                1442    1158    1087     583      65
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1519    4335 
   Size:       1519    2816
 
SmlI C'TyrA_G

Cuts at:      0     841    2423    3569    4335 
   Size:        841    1582    1146     766

  Fragments arranged by size: 

                1582    1146     841     766
 
SphI G_CATG'C

Cuts at:      0    3228    4335 
   Size:       3228    1107
 
SspI AAT'ATT

Cuts at:      0    1343    4335 
   Size:       1343    2992
 
StuI AGG'CCT

Cuts at:      0    1672    1994    3820    4018    4170    4335 
   Size:       1672     322    1826     198     152     165

  Fragments arranged by size: 

                1826    1672     322     198     165     152
 
StyI C'CwwG_G

Cuts at:      0     596    1347    1472    1673    1712    2309    2772    3026 
   Size:        596     751     125     201      39     597     463     254

Cuts at:   3026    4335 
   Size:       1309

  Fragments arranged by size: 

                1309     751     597     596     463     254     201     125
                  39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1062    1139    1143    2035    2129    2785    4335 
   Size:       1062      77       4     892      94     656    1550

  Fragments arranged by size: 

                1550    1062     892     656      94      77       4
 
TatI w'GTAC_w

Cuts at:      0    2572    3111    3323    3782    4335 
   Size:       2572     539     212     459     553

  Fragments arranged by size: 

                2572     553     539     459     212
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1371    1403    2133    2165    2825    2857    4335 
   Size:       1371      32     730      32     660      32    1478

  Fragments arranged by size: 

                1478    1371     730     660      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     883    2008    3919    4335 
   Size:        883    1125    1911     416

  Fragments arranged by size: 

                1911    1125     883     416
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1671    4335 
   Size:       1671    2664
 
XhoI C'TCGA_G

Cuts at:      0    2423    3569    4335 
   Size:       2423    1146     766

  Fragments arranged by size: 

                2423    1146     766
 
XmnI GAAnn'nnTTC

Cuts at:      0    2444    2480    3356    3543    3697    4335 
   Size:       2444      36     876     187     154     638

  Fragments arranged by size: 

                2444     876     638     187     154      36
 
ZraI GAC'GTC

Cuts at:      0    1442    4335 
   Size:       1442    2893
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII
     HpaI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NspI     PasI    PflMI     PfoI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PstI    PvuII     SacI    SacII     SapI     ScaI
     SfcI     SfiI     SmlI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AloI     AscI    AsiSI
    AvrII     BbeI    BciVI    BfrBI    BglII     BmtI    BsaAI    BsaBI
    BsiWI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI    EcoRI    EcoRV     FseI    FspAI  HindIII     KasI     KpnI
     MfeI     MluI     NarI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI    PshAI     PsrI     PvuI    RsrII     SalI
    SanDI     SbfI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SwaI     XbaI     XmaI
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