Restriction map for pF1KB5499
##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AatII       Acc65I      AclI        AhdI        AscI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BlnI        BsiWI       BspLU11I    BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CpoI        DraI        
Eam1105I    EcoT22I     FseI        FspI        HpaI        
KpnI        MluI        NheI        NruI        NsiI        
NspV        PacI        PmeI        Psp1406I    PvuI        
RsrII       SalI        SbfI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          940  GTAGAC               GT^[AC][GT]AC
AccIII       3359  TCCGGA               T^CCGGA
AflII        1803  CTTAAG               C^TTAAG
AgeI         2585  ACCGGT               A^CCGGT
AocI         2959  CCTGAGG              CC^T[ACGT]AGG
ApaI          508  GGGCCC               GGGCC^C
AseI         4549  ATTAAT               AT^TAAT
AsnI         4549  ATTAAT               AT^TAAT
Asp700I      1152  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1399  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         1126  GGCGCC               GGCGC^C
BbvCI        4539  CCTCAGC              CCTCAGC(-5/-2)
BcgI         4497  CGAGATCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3129  TGATCA               T^GATCA
BfrI         1803  CTTAAG               C^TTAAG
BsaBI        3432  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3359  TCCGGA               T^CCGGA
BsmBI         445  CGTCTC               CGTCTC(1/5)
Bsp1407I     1510  TGTACA               T^GTACA
BspDI        2500  ATCGAT               AT^CGAT
BspEI        3359  TCCGGA               T^CCGGA
BspHI        4745  TCATGA               T^CATGA
BsrBI        3424  GAGCGG               CCGCTC(-3/-3)
BsrGI        1510  TGTACA               T^GTACA
BstXI        2739  CCAGGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2959  CCTGAGG              CC^T[ACGT]AGG
ClaI         2500  ATCGAT               AT^CGAT
DrdI         2147  GACCATACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2959  CCTGAGG              CC^T[ACGT]AGG
EcoRI         622  GAATTC               G^AATTC
FbaI         3129  TGATCA               T^GATCA
KasI         1126  GGCGCC               G^GCGCC
MamI         3432  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3359  TCCGGA               T^CCGGA
NaeI           24  GCCGGC               GCC^GGC
NarI         1126  GGCGCC               GG^CGCC
NdeI          932  CATATG               CA^TATG
NgoMIV         24  GCCGGC               G^CCGGC
NspI          149  GCATGC               [GA]CATG^[CT]
PaeR7I       1612  CTCGAG               C^TCGAG
PflFI        1399  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2585  ACCGGT               A^CCGGT
PshAI         246  GACCGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4549  ATTAAT               AT^TAAT
RcaI         4745  TCATGA               T^CATGA
SacI         2413  GAGCTC               GAGCT^C
ScaI          527  AGTACT               AGT^ACT
SexAI         346  ACCTGGT              A^CC[AT]GGT
SfoI         1126  GGCGCC               GGC^GCC
SmaI           38  CCCGGG               CCC^GGG
SphI          149  GCATGC               GCATG^C
SspBI        1510  TGTACA               T^GTACA
Tth111I      1399  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1612  CTCGAG               C^TCGAG
XmaCI          38  CCCGGG               C^CCGGG
XmaI           38  CCCGGG               C^CCGGG
XmnI         1152  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          277  GACGCC               G[GA]^CG[CT]C
             1126  GGCGCC              
AflIII        200  ACACGT               A^C[GA][CT]GT
              202  ACGTGT              
Alw44I        117  GTGCAC               G^TGCAC
             2373  GTGCAC              
ApaLI         117  GTGCAC               G^TGCAC
             2373  GTGCAC              
BbrPI         201  CACGTG               CAC^GTG
             2728  CACGTG              
BciVI        4071  GGATAC               GTATCC(6/5)
             4581  GGATAC              
BfaI         4015  CTAG                 C^TAG
             4532  CTAG                
BglII        3797  AGATCT               A^GATCT
             4365  AGATCT              
BlpI         3814  GCTCAGC              GC^T[ACGT]AGC
             3819  GCTGAGC             
Bpu1102I     3814  GCTCAGC              GC^T[ACGT]AGC
             3819  GCTGAGC             
BsaHI         277  GACGCC               G[GA]^CG[CT]C
             1126  GGCGCC              
BsaWI        2585  ACCGGT               [AT]^CCGG[AT]
             3359  TCCGGA              
BstEII        243  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
BstPI         243  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
CelII        3814  GCTCAGC              GC^T[ACGT]AGC
             3819  GCTGAGC             
EcoO65I       243  GGTGACC              G^GT[ACGT]ACC
              252  GGTCACC             
EcoRV        1636  GATATC               GAT^ATC
             4732  GATATC              
Hin1I         277  GACGCC               G[GA]^CG[CT]C
             1126  GGCGCC              
HindIII       422  AAGCTT               A^AGCTT
             3553  AAGCTT              
KspI           83  CCGCGG               CCGC^GG
              570  CCGCGG              
MaeI         4015  CTAG                 C^TAG
             4532  CTAG                
MfeI         1416  CAATTG               C^AATTG
             2652  CAATTG              
MunI         1416  CAATTG               C^AATTG
             2652  CAATTG              
NcoI          107  CCATGG               C^CATGG
             1086  CCATGG              
NotI           76  GCGGCCGC             GC^GGCCGC
              135  GCGGCCGC            
PflMI         344  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2811  CCATGCCTTGG         
PmaCI         201  CACGTG               CAC^GTG
             2728  CACGTG              
PmlI          201  CACGTG               CAC^GTG
             2728  CACGTG              
PpuMI        3512  GGGACCC              [GA]G^G[AT]CC[CT]
             3923  AGGACCT             
SacII          83  CCGCGG               CCGC^GG
              570  CCGCGG              
SapI         3688  GAAGAGC              GCTCTTC(1/4)
             1184  GCTCTTC             
StuI          605  AGGCCT               AGG^CCT
             1906  AGGCCT              
Van91I        344  CCACCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2811  CCATGCCTTGG         
XcmI          525  CCAGTACTACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2913  CCACTTTGGGTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com