Restriction map for pF1KB4514
##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       AflII       AgeI        Aor51HI     AscI        
AseI        AsnI        Asp700I     AvrII       BfrI        
BlnI        BsaBI       BspDI       BsrBI       BstBI       
ClaI        CpoI        DrdI        Eco47III    EcoRI       
EcoRV       EcoT22I     HindIII     HpaI        MamI        
MfeI        MluI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshBI       PvuI        RsrII       
SalI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SpeI        SphI        Sse8387I    SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       4704  GGTACC               G^GTACC
AclI         4630  AACGTT               AA^CGTT
Alw44I       1051  GTGCAC               G^TGCAC
ApaLI        1051  GTGCAC               G^TGCAC
Asp718I      4704  GGTACC               G^GTACC
AspI          363  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         395  TGCGCA               TGC^GCA
BcgI         3681  GCACGTGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4815  GGATAC               GTATCC(6/5)
BlpI         3237  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3237  GCTGAGC              GC^T[ACGT]AGC
BsiEI        4406  CGGCCG               CG[GA][CT]^CG
BsiWI        4517  CGTACG               C^GTACG
BspLU11I     2059  ACATGT               A^CATGT
BssHII         81  GCGCGC               G^CGCGC
Bst1107I     4072  GTATAC               GTA^TAC
BstZ17I      4072  GTATAC               GTA^TAC
CelII        3237  GCTGAGC              GC^T[ACGT]AGC
DraI         2907  TTTAAA               TTT^AAA
DraIII       2263  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         4406  CGGCCG               C^GGCCG
EclXI        4406  CGGCCG               C^GGCCG
Eco52I       4406  CGGCCG               C^GGCCG
FseI         4403  GGCCGGCC             GGCCGG^CC
FspI          395  TGCGCA               TGC^GCA
HincII        166  GTTGAC               GT[CT]^[GA]AC
HindII        166  GTTGAC               GT[CT]^[GA]AC
KpnI         4704  GGTACC               GGTAC^C
NheI          270  GCTAGC               G^CTAGC
PflFI         363  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2977  GACGAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4630  AACGTT               AA^CGTT
SapI          186  GCTCTTC              GCTCTTC(1/4)
SfiI         2541  GGCCTACGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI        2476  TACGTA               TAC^GTA
Tth111I       363  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccIII       2620  TCCGGA               T^CCGGA
             3406  TCCGGA              
AcsI         2004  AAATTC               [GA]^AATT[CT]
             3517  AAATTC              
AhdI         3607  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3931  GACACATGGTC         
ApoI         2004  AAATTC               [GA]^AATT[CT]
             3517  AAATTC              
AspEI        3607  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3931  GACACATGGTC         
BalI         3662  TGGCCA               TGG^CCA
             3794  TGGCCA              
BbeI          312  GGCGCC               GGCGC^C
              869  GGCGCC              
BbrPI        3682  CACGTG               CAC^GTG
             4423  CACGTG              
BbsI         3447  GAAGAC               GAAGAC(2/6)
             4111  GAAGAC              
BbvCI         974  CCTCAGC              CCTCAGC(-5/-2)
             3043  GCTGAGG             
BclI         2513  TGATCA               T^GATCA
             3506  TGATCA              
BglII         653  AGATCT               A^GATCT
             2171  AGATCT              
BpuAI        3447  GAAGAC               GAAGAC(2/6)
             4111  GAAGAC              
BseAI        2620  TCCGGA               T^CCGGA
             3406  TCCGGA              
BsmBI        1044  CGTCTC               CGTCTC(1/5)
             3054  CGTCTC              
BsmI         3410  GAATGC               GAATGC(1/-1)
             4435  GCATTC              
Bsp1407I     2312  TGTACA               T^GTACA
             2894  TGTACA              
BspEI        2620  TCCGGA               T^CCGGA
             3406  TCCGGA              
BspHI        3164  TCATGA               T^CATGA
             3263  TCATGA              
BsrGI        2312  TGTACA               T^GTACA
             2894  TGTACA              
BssSI        4582  CACGAG               CACGAG(-5/-1)
             3130  CTCGTG              
BstEII       3117  GGTGACC              G^GT[ACGT]ACC
             4591  GGTAACC             
BstPI        3117  GGTGACC              G^GT[ACGT]ACC
             4591  GGTAACC             
Eam1105I     3607  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3931  GACACATGGTC         
EcoO65I      3117  GGTGACC              G^GT[ACGT]ACC
             4591  GGTAACC             
FbaI         2513  TGATCA               T^GATCA
             3506  TGATCA              
HaeII         312  GGCGCC               [GA]GCGC^[CT]
              869  GGCGCC              
KasI          312  GGCGCC               G^GCGCC
              869  GGCGCC              
MluNI        3662  TGGCCA               TGG^CCA
             3794  TGGCCA              
MroI         2620  TCCGGA               T^CCGGA
             3406  TCCGGA              
MscI         3662  TGGCCA               TGG^CCA
             3794  TGGCCA              
NaeI          309  GCCGGC               GCC^GGC
             4404  GCCGGC              
NarI          312  GGCGCC               GG^CGCC
              869  GGCGCC              
NcoI         2079  CCATGG               C^CATGG
             3369  CCATGG              
NgoMIV        309  GCCGGC               G^CCGGC
             4404  GCCGGC              
PflMI        3778  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4499  CCAGCTTGTGG         
PmaCI        3682  CACGTG               CAC^GTG
             4423  CACGTG              
PmlI         3682  CACGTG               CAC^GTG
             4423  CACGTG              
RcaI         3164  TCATGA               T^CATGA
             3263  TCATGA              
SfoI          312  GGCGCC               GGC^GCC
              869  GGCGCC              
SspBI        2312  TGTACA               T^GTACA
             2894  TGTACA              
SspI         2938  AATATT               AAT^ATT
             3912  AATATT              
Van91I       3778  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4499  CCAGCTTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       BamHI       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com