Restriction map for pF1KB4514
 (Linear) (Six-base) MAPSORT of: KIBB4514.seq  Check: 2377  from: 1  to: 4860



KIBB4514.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1070    2620    3098    4860 
   Size:       1070    1550     478    1762

  Fragments arranged by size: 

                1762    1550    1070     478
 
AccI GT'mk_AC

Cuts at:      0     197    2975    4073    4860 
   Size:        197    2778    1098     787

  Fragments arranged by size: 

                2778    1098     787     197
 
Acc65I G'GTAC_C

Cuts at:      0    4704    4860 
   Size:       4704     156
 
AclI AA'CG_TT

Cuts at:      0    4631    4860 
   Size:       4631     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     400    1285    3343    3479    4130    4860 
   Size:        259     141     885    2058     136     651     730

  Fragments arranged by size: 

                2058     885     730     651     259     141     136
 
AflIII A'CryG_T

Cuts at:      0    1688    2059    2561    4422    4860 
   Size:       1688     371     502    1861     438

  Fragments arranged by size: 

                1861    1688     502     438     371
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3612    3936    4860 
   Size:       3612     324     924

  Fragments arranged by size: 

                3612     924     324
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2711    4860 
   Size:       2711    2149
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     321     353    1513    1545    3473    3505    4860 
   Size:        321      32    1160      32    1928      32    1355

  Fragments arranged by size: 

                1928    1355    1160     321      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1095    1820    2966    3195    3761    3784    4860 
   Size:       1095     725    1146     229     566      23    1076

  Fragments arranged by size: 

                1146    1095    1076     725     566     229      23
 
ApaI G_GGCC'C

Cuts at:      0     470    2078    2155    4859    4860 
   Size:        470    1608      77    2704       1

  Fragments arranged by size: 

                2704    1608     470      77       1
 
ApaLI G'TGCA_C

Cuts at:      0    1051    4860 
   Size:       1051    3809
 
ApoI r'AATT_y

Cuts at:      0    2004    3517    4860 
   Size:       2004    1513    1343

  Fragments arranged by size: 

                2004    1513    1343
 
AvaI C'yCGr_G

Cuts at:      0     119     203     253     472     571    1996    2208    3642 
   Size:        119      84      50     219      99    1425     212    1434

Cuts at:   3642    3689    3821    4860 
   Size:         47     132    1039

  Fragments arranged by size: 

                1434    1425    1039     219     212     132     119      99
                  84      50      47
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4696    4729    4860 
   Size:       4696      33     131

  Fragments arranged by size: 

                4696     131      33
 
BamHI G'GATC_C

Cuts at:      0     557    1852    4480    4860 
   Size:        557    1295    2628     380

  Fragments arranged by size: 

                2628    1295     557     380
 
BanI G'GyrC_C

Cuts at:      0     177     312     869    1058    2181    2505    2695    3330 
   Size:        177     135     557     189    1123     324     190     635

Cuts at:   3330    4704    4860 
   Size:       1374     156

  Fragments arranged by size: 

                1374    1123     635     557     324     190     189     177
                 156     135
 
BanII G_rGCy'C

Cuts at:      0      17      61      91     470    1318    1636    1649    2078 
   Size:         17      44      30     379     848     318      13     429

Cuts at:   2078    2155    3226    3593    4676    4859    4860 
   Size:         77    1071     367    1083     183       1

  Fragments arranged by size: 

                1083    1071     848     429     379     367     318     183
                  77      44      30      17      13       1
 
BbeI G_GCGC'C

Cuts at:      0     316     873    4860 
   Size:        316     557    3987

  Fragments arranged by size: 

                3987     557     316
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3454    4118    4860 
   Size:       3454     664     742

  Fragments arranged by size: 

                3454     742     664
 
BbvCI CC'TCA_GC

Cuts at:      0     975    3044    4860 
   Size:        975    2069    1816

  Fragments arranged by size: 

                2069    1816     975
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3670    3704    4860 
   Size:       3670      34    1156

  Fragments arranged by size: 

                3670    1156      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    4809    4860 
   Size:       4809      51
 
BclI T'GATC_A

Cuts at:      0    2513    3506    4860 
   Size:       2513     993    1354

  Fragments arranged by size: 

                2513    1354     993
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1867    2548    3133    4239    4860 
   Size:       1867     681     585    1106     621

  Fragments arranged by size: 

                1867    1106     681     621     585
 
BglII A'GATC_T

Cuts at:      0     653    2171    4860 
   Size:        653    1518    2689

  Fragments arranged by size: 

                2689    1518     653
 
BlpI GC'TnA_GC

Cuts at:      0    3238    4860 
   Size:       3238    1622
 
Bme1580I G_kGCm'C

Cuts at:      0     182     470    1055    1386    2078    2155    2510    4859 
   Size:        182     288     585     331     692      77     355    2349

Cuts at:   4859    4860 
   Size:          1

  Fragments arranged by size: 

                2349     692     585     355     331     288     182      77
                   1
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2345    2893    4649    4860 
   Size:       2345     548    1756     211

  Fragments arranged by size: 

                2345    1756     548     211
 
BmtI G_CTAG'C

Cuts at:      0     274    4860 
   Size:        274    4586
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     909    1141    2607    4860 
   Size:        909     232    1466    2253

  Fragments arranged by size: 

                2253    1466     909     232
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     481     811     841    1124    1150    1206    1330 
   Size:        448      33     330      30     283      26      56     124

Cuts at:   1330    2314    2602    3607    4036    4624    4817    4860 
   Size:        984     288    1005     429     588     193      43

  Fragments arranged by size: 

                1005     984     588     448     429     330     288     283
                 193     124      56      43      33      30      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     295     975    2128    3044    4860 
   Size:        295     680    1153     916    1816

  Fragments arranged by size: 

                1816    1153     916     680     295
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1698    1788    3595    4860 
   Size:       1698      90    1807    1265

  Fragments arranged by size: 

                1807    1698    1265      90
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     327    3599    3622    4281    4386    4860 
   Size:        327    3272      23     659     105     474

  Fragments arranged by size: 

                3272     659     474     327     105      23
 
BsaAI yAC'GTr

Cuts at:      0    2478    3684    4425    4860 
   Size:       2478    1206     741     435

  Fragments arranged by size: 

                2478    1206     741     435
 
BsaHI Gr'CG_yC

Cuts at:      0     313     583     870    1182    2342    3053    4860 
   Size:        313     270     287     312    1160     711    1807

  Fragments arranged by size: 

                1807    1160     711     313     312     287     270
 
BsaWI w'CCGG_w

Cuts at:      0    2620    2701    3406    4076    4860 
   Size:       2620      81     705     670     784

  Fragments arranged by size: 

                2620     784     705     670      81
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1008    1038    1867    1897    2104    2134    2489    2519 
   Size:       1008      30     829      30     207      30     355      30

Cuts at:   2519    4860 
   Size:       2341

  Fragments arranged by size: 

                2341    1008     829     355     207      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     219     454     901    1029    1484    1789    2468    2471 
   Size:        219     235     447     128     455     305     679       3

Cuts at:   2471    2583    2775    2810    3297    3645    4256    4312    4315 
   Size:        112     192      35     487     348     611      56       3

Cuts at:   4315    4332    4860 
   Size:         17     528

  Fragments arranged by size: 

                 679     611     528     487     455     447     348     305
                 235     219     192     128     112      56      35      17
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      93     303     432     632    1088    2277    2901    3991 
   Size:         93     210     129     200     456    1189     624    1090

Cuts at:   3991    4311    4860 
   Size:        320     549

  Fragments arranged by size: 

                1189    1090     624     549     456     320     210     200
                 129      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     816    1017    1604    3664    4140    4860 
   Size:        816     201     587    2060     476     720

  Fragments arranged by size: 

                2060     816     720     587     476     201
 
BsiEI CG_ry'CG

Cuts at:      0    4409    4860 
   Size:       4409     451
 
BsiHKAI G_wGCw'C

Cuts at:      0    1031    1055    1166    1318    1636    1649    2785    3469 
   Size:       1031      24     111     152     318      13    1136     684

Cuts at:   3469    3593    4860 
   Size:        124    1267

  Fragments arranged by size: 

                1267    1136    1031     684     318     152     124     111
                  24      13
 
BsiWI C'GTAC_G

Cuts at:      0    4517    4860 
   Size:       4517     343
 
BsmI GAATG_Cn'

Cuts at:      0    3416    4435    4860 
   Size:       3416    1019     425

  Fragments arranged by size: 

                3416    1019     425
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1050    3060    4860 
   Size:       1050    2010    1800

  Fragments arranged by size: 

                2010    1800    1050
 
Bsp1286I G_dGCh'C

Cuts at:      0      17      61      91     182     470    1031    1055    1166 
   Size:         17      44      30      91     288     561      24     111

Cuts at:   1166    1318    1386    1636    1649    2078    2155    2510    2785 
   Size:        152      68     250      13     429      77     355     275

Cuts at:   2785    3226    3469    3593    4676    4859    4860 
   Size:        441     243     124    1083     183       1

  Fragments arranged by size: 

                1083     561     441     429     355     288     275     250
                 243     183     152     124     111      91      77      68
                  44      30      24      17      13       1
 
BspEI T'CCGG_A

Cuts at:      0    2620    3406    4860 
   Size:       2620     786    1454

  Fragments arranged by size: 

                2620    1454     786
 
BspHI T'CATG_A

Cuts at:      0    3164    3263    4860 
   Size:       3164      99    1597

  Fragments arranged by size: 

                3164    1597      99
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1070    1494    2620    3098    4860 
   Size:       1070     424    1126     478    1762

  Fragments arranged by size: 

                1762    1126    1070     478     424
 
BsrDI GCAATG_nn'

Cuts at:      0    1082    2516    2826    3695    3746    4860 
   Size:       1082    1434     310     869      51    1114

  Fragments arranged by size: 

                1434    1114    1082     869     310      51
 
BsrFI r'CCGG_y

Cuts at:      0      73     309     334    2502    4404    4860 
   Size:         73     236      25    2168    1902     456

  Fragments arranged by size: 

                2168    1902     456     236      73      25
 
BsrGI T'GTAC_A

Cuts at:      0    2312    2894    4860 
   Size:       2312     582    1966

  Fragments arranged by size: 

                2312    1966     582
 
BssHII G'CGCG_C

Cuts at:      0      81    4860 
   Size:         81    4779
 
BssSI C'ACGA_G

Cuts at:      0    3130    4582    4860 
   Size:       3130    1452     278

  Fragments arranged by size: 

                3130    1452     278
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     423    3204    3567    4860 
   Size:        423    2781     363    1293

  Fragments arranged by size: 

                2781    1293     423     363
 
BstEII G'GTnAC_C

Cuts at:      0    3117    4591    4860 
   Size:       3117    1474     269

  Fragments arranged by size: 

                3117    1474     269
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     420    1893    3210    3881    4137    4860 
   Size:        420    1473    1317     671     256     723

  Fragments arranged by size: 

                1473    1317     723     671     420     256
 
BstYI r'GATC_y

Cuts at:      0     557     653    1121    1852    1928    2171    4480    4860 
   Size:        557      96     468     731      76     243    2309     380

  Fragments arranged by size: 

                2309     731     557     468     380     243      96      76
            
 
BstZ17I GTA'TAC

Cuts at:      0    4074    4860 
   Size:       4074     786
 
Bsu36I CC'TnA_GG

Cuts at:      0     847    2787    4253    4860 
   Size:        847    1940    1466     607

  Fragments arranged by size: 

                1940    1466     847     607
 
BtgI C'CryG_G

Cuts at:      0      44     261     614    1006    2079    2262    2925    3027 
   Size:         44     217     353     392    1073     183     663     102

Cuts at:   3027    3334    3369    4409    4476    4860 
   Size:        307      35    1040      67     384

  Fragments arranged by size: 

                1073    1040     663     392     384     353     307     217
                 183     102      67      44      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     757    4860 
   Size:        757    4103
 
BtsI GCAGTG_nn'

Cuts at:      0     835    1029    1253    1281    1786    4660    4860 
   Size:        835     194     224      28     505    2874     200

  Fragments arranged by size: 

                2874     835     505     224     200     194      28
 
DraI TTT'AAA

Cuts at:      0    2909    4860 
   Size:       2909    1951
 
DraIII CAC_nnn'GTG

Cuts at:      0    2268    4860 
   Size:       2268    2592
 
EaeI y'GGCC_r

Cuts at:      0      75    1040    3134    3662    3794    4406    4860 
   Size:         75     965    2094     528     132     612     454

  Fragments arranged by size: 

                2094     965     612     528     454     132      75
 
EagI C'GGCC_G

Cuts at:      0    4406    4860 
   Size:       4406     454
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     193     652    3783    4211    4217    4683    4860 
   Size:        148      45     459    3131     428       6     466     177

  Fragments arranged by size: 

                3131     466     459     428     177     148      45       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     550    2465    2816    4860 
   Size:        550    1915     351    2044

  Fragments arranged by size: 

                2044    1915     550     351
 
EcoICRI GAG'CTC

Cuts at:      0    1316    1634    1647    3591    4860 
   Size:       1316     318      13    1944    1269

  Fragments arranged by size: 

                1944    1316    1269     318      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     400     448     481     811     841    1124    1150 
   Size:        259     141      48      33     330      30     283      26

Cuts at:   1150    1206    1285    1330    2314    2602    3343    3479    3607 
   Size:         56      79      45     984     288     741     136     128

Cuts at:   3607    4036    4130    4624    4817    4860 
   Size:        429      94     494     193      43

  Fragments arranged by size: 

                 984     741     494     429     330     288     283     259
                 193     141     136     128      94      79      56      48
                  45      43      33      30      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1074    2296    3325    4860 
   Size:       1074    1222    1029    1535

  Fragments arranged by size: 

                1535    1222    1074    1029
 
EcoO109I rG'GnC_Cy

Cuts at:      0     207     467    1511    2075    2151    2152    2540    3781 
   Size:        207     260    1044     564      76       1     388    1241

Cuts at:   3781    4164    4248    4655    4860 
   Size:        383      84     407     205

  Fragments arranged by size: 

                1241    1044     564     407     388     383     260     207
                 205      84      76       1
 
FseI GG_CCGG'CC

Cuts at:      0    4408    4860 
   Size:       4408     452
 
FspI TGC'GCA

Cuts at:      0     397    4860 
   Size:        397    4463
 
HaeII r_GCGC'y

Cuts at:      0     316     873    4860 
   Size:        316     557    3987

  Fragments arranged by size: 

                3987     557     316
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     141     173     542     574    1122    1154    1867    1899 
   Size:        141      32     369      32     548      32     713      32

Cuts at:   1899    3040    3072    3598    3621    3630    3653    3922    3954 
   Size:       1141      32     526      23       9      23     269      32

Cuts at:   3954    4071    4103    4860 
   Size:        117      32     757

  Fragments arranged by size: 

                1141     757     713     548     526     369     269     141
                 117      32      32      32      32      32      32      32
                  23      23       9
 
HincII GTy'rAC

Cuts at:      0     168    4860 
   Size:        168    4692
 
KasI G'GCGC_C

Cuts at:      0     312     869    4860 
   Size:        312     557    3991

  Fragments arranged by size: 

                3991     557     312
 
KpnI G_GTAC'C

Cuts at:      0    4708    4860 
   Size:       4708     152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     566    1135    1309    2147    4472    4860 
   Size:        566     569     174     838    2325     388

  Fragments arranged by size: 

                2325     838     569     566     388     174
 
MscI TGG'CCA

Cuts at:      0    3664    3796    4860 
   Size:       3664     132    1064

  Fragments arranged by size: 

                3664    1064     132
 
MslI CAynn'nnrTG

Cuts at:      0    2251    3169    3208    3550    4860 
   Size:       2251     918      39     342    1310

  Fragments arranged by size: 

                2251    1310     918     342      39
 
MspA1I CmG'CkG

Cuts at:      0      33      52      97     111     263     291     616     979 
   Size:         33      19      45      14     152      28     325     363

Cuts at:    979    1092    2072    3336    4315    4372    4860 
   Size:        113     980    1264     979      57     488

  Fragments arranged by size: 

                1264     980     979     488     363     325     152     113
                  57      45      33      28      19      14
 
NaeI GCC'GGC

Cuts at:      0     311    4406    4860 
   Size:        311    4095     454

  Fragments arranged by size: 

                4095     454     311
 
NarI GG'CG_CC

Cuts at:      0     313     870    4860 
   Size:        313     557    3990

  Fragments arranged by size: 

                3990     557     313
 
NcoI C'CATG_G

Cuts at:      0    2079    3369    4860 
   Size:       2079    1290    1491

  Fragments arranged by size: 

                2079    1491    1290
 
NgoMIV G'CCGG_C

Cuts at:      0     309    4404    4860 
   Size:        309    4095     456

  Fragments arranged by size: 

                4095     456     309
 
NheI G'CTAG_C

Cuts at:      0     270    4860 
   Size:        270    4590
 
NspI r_CATG'y

Cuts at:      0     699    1750    2063    2310    4860 
   Size:        699    1051     313     247    2550

  Fragments arranged by size: 

                2550    1051     699     313     247
 
PasI CC'CwG_GG

Cuts at:      0    1386    4063    4860 
   Size:       1386    2677     797

  Fragments arranged by size: 

                2677    1386     797
 
PciI A'CATG_T

Cuts at:      0    2059    4860 
   Size:       2059    2801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3784    4505    4860 
   Size:       3784     721     355

  Fragments arranged by size: 

                3784     721     355
 
PfoI T'CCnGG_A

Cuts at:      0      61     325    1532    4860 
   Size:         61     264    1207    3328

  Fragments arranged by size: 

                3328    1207     264      61
 
PmlI CAC'GTG

Cuts at:      0    3684    4425    4860 
   Size:       3684     741     435

  Fragments arranged by size: 

                3684     741     435
 
PpuMI rG'GwC_Cy

Cuts at:      0     207    1511    4164    4248    4860 
   Size:        207    1304    2653      84     612

  Fragments arranged by size: 

                2653    1304     612     207      84
 
PshAI GACnn'nnGTC

Cuts at:      0    2981    4860 
   Size:       2981    1879
 
PspOMI G'GGCC_C

Cuts at:      0     466    2074    2151    4855    4860 
   Size:        466    1608      77    2704       5

  Fragments arranged by size: 

                2704    1608     466      77       5
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4501    4533    4860 
   Size:       4501      32     327

  Fragments arranged by size: 

                4501     327      32
 
PstI C_TGCA'G

Cuts at:      0     116     149    1067    1248    1276    1313    1829    1937 
   Size:        116      33     918     181      28      37     516     108

Cuts at:   1937    2962    3221    4377    4860 
   Size:       1025     259    1156     483

  Fragments arranged by size: 

                1156    1025     918     516     483     259     181     116
                 108      37      33      28
 
PvuII CAG'CTG

Cuts at:      0      52      97     979    1092    4315    4372    4860 
   Size:         52      45     882     113    3223      57     488

  Fragments arranged by size: 

                3223     882     488     113      57      52      45
 
SacI G_AGCT'C

Cuts at:      0    1318    1636    1649    3593    4860 
   Size:       1318     318      13    1944    1267

  Fragments arranged by size: 

                1944    1318    1267     318      13
 
SacII CC_GC'GG

Cuts at:      0     264     617    3337    4860 
   Size:        264     353    2720    1523

  Fragments arranged by size: 

                2720    1523     353     264
 
SapI GCTCTTCn'nnn_

Cuts at:      0     193    4860 
   Size:        193    4667
 
SfcI C'TryA_G

Cuts at:      0     112     145    1063    1244    1272    1309    1595    1825 
   Size:        112      33     918     181      28      37     286     230

Cuts at:   1825    1933    2036    2199    2958    3217    3831    3846    4373 
   Size:        108     103     163     759     259     614      15     527

Cuts at:   4373    4562    4860 
   Size:        189     298

  Fragments arranged by size: 

                 918     759     614     527     298     286     259     230
                 189     181     163     112     108     103      37      33
                  28      15
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2548    4860 
   Size:       2548    2312
 
SfoI GGC'GCC

Cuts at:      0     314     871    4860 
   Size:        314     557    3989

  Fragments arranged by size: 

                3989     557     314
 
SmaI CCC'GGG

Cuts at:      0     121     205     474    1998    4860 
   Size:        121      84     269    1524    2862

  Fragments arranged by size: 

                2862    1524     269     121      84
 
SmlI C'TyrA_G

Cuts at:      0    1677    1767    3610    4860 
   Size:       1677      90    1843    1250

  Fragments arranged by size: 

                1843    1677    1250      90
 
SnaBI TAC'GTA

Cuts at:      0    2478    4860 
   Size:       2478    2382
 
SspI AAT'ATT

Cuts at:      0    2940    3914    4860 
   Size:       2940     974     946

  Fragments arranged by size: 

                2940     974     946
 
StuI AGG'CCT

Cuts at:      0     892    1360    3898    4860 
   Size:        892     468    2538     962

  Fragments arranged by size: 

                2538     962     892     468
 
StyI C'CwwG_G

Cuts at:      0     537     677    1470    1694    2079    3369    4244    4860 
   Size:        537     140     793     224     385    1290     875     616

  Fragments arranged by size: 

                1290     875     793     616     537     385     224     140
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1962    2104    2585    3087    3283    4065    4174    4860 
   Size:       1962     142     481     502     196     782     109     686

  Fragments arranged by size: 

                1962     782     686     502     481     196     142     109
            
 
TatI w'GTAC_w

Cuts at:      0    2312    2353    2894    3230    4615    4860 
   Size:       2312      41     541     336    1385     245

  Fragments arranged by size: 

                2312    1385     541     336     245      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1008    1040    2489    2521    2873    2905    4860 
   Size:       1008      32    1449      32     352      32    1955

  Fragments arranged by size: 

                1955    1449    1008     352      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     366    4860 
   Size:        366    4494
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51    1863    2130    3376    4860 
   Size:         51    1812     267    1246    1484

  Fragments arranged by size: 

                1812    1484    1246     267      51
 
XmaI C'CCGG_G

Cuts at:      0     119     203     472    1996    4860 
   Size:        119      84     269    1524    2864

  Fragments arranged by size: 

                2864    1524     269     119      84
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FseI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII     SapI     SfcI     SfiI     SfoI     SmaI     SmlI    SnaBI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AleI     AscI     AseI    AsiSI
    AvrII    BfrBI    BmgBI    BsaBI    BsrBI    BstBI     ClaI    CspCI
     DrdI    EcoRI    EcoRV     FalI    FspAI  HindIII     HpaI     MfeI
     MluI     NdeI     NotI     NruI     NsiI     PacI     PmeI     PpiI
     PsiI    PspXI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SpeI     SphI     SrfI     SwaI     XbaI
     XhoI     XmnI     ZraI
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