Restriction map for pF1KB4032
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      ApaLI       Asp718I     AspI        
AvaI        AviII       BciVI       BclI        BfrI        
BsaWI       BseAI       BsiEI       BsiWI       BsoBI       
BspDI       BspEI       BsrBI       BssSI       BstBI       
BstEII      BstPI       ClaI        CpoI        DrdI        
EagI        EclXI       Eco52I      EcoO65I     EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MluI        MroI        MslI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AcyI         1869  GGCGCC               G[GA]^CG[CT]C
AhdI          781  GACAATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2518  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       479  AGCGCT               AGC^GCT
ApaI         2659  GGGCCC               GGGCC^C
AscI         3315  GGCGCGCC             GG^CGCGCC
AseI         1543  ATTAAT               AT^TAAT
AsnI         1543  ATTAAT               AT^TAAT
Asp700I      2089  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         781  GACAATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         382  CCTAGG               C^CTAGG
BalI         1704  TGGCCA               TGG^CCA
BamHI         309  GGATCC               G^GATCC
BanI         1869  GGCGCC               G^G[CT][GA]CC
BbeI         1869  GGCGCC               GGCGC^C
BbrPI         613  CACGTG               CAC^GTG
BcgI         1670  CGAGAGTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          382  CCTAGG               C^CTAGG
BsaBI        1006  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1869  GGCGCC               G[GA]^CG[CT]C
BseRI        2608  GAGGAG               GAGGAG(10/8)
BsmBI         104  GAGACG               CGTCTC(1/5)
Bsp1407I     2841  TGTACA               T^GTACA
BspHI         261  TCATGA               T^CATGA
BsrDI         635  GCAATG               GCAATG(2/0)
BsrFI         947  ACCGGC               [GA]^CCGG[CT]
BsrGI        2841  TGTACA               T^GTACA
BssHII       3316  GCGCGC               G^CGCGC
Bst1107I     1854  GTATAC               GTA^TAC
BstXI        1236  CCATCCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1854  GTATAC               GTA^TAC
Bsu36I       2518  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        947  ACCGGC               [GA]^CCGG[CT]
DraI          318  TTTAAA               TTT^AAA
DraIII       3138  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      781  GACAATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      479  AGCGCT               AGC^GCT
Eco81I       2518  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2093  GAATTC               G^AATTC
EcoRV        1465  GATATC               GAT^ATC
Hin1I        1869  GGCGCC               G[GA]^CG[CT]C
HindIII      2269  AAGCTT               A^AGCTT
KasI         1869  GGCGCC               G^GCGCC
MamI         1006  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          457  CAATTG               C^AATTG
MluNI        1704  TGGCCA               TGG^CCA
MscI         1704  TGGCCA               TGG^CCA
MunI          457  CAATTG               C^AATTG
NarI         1869  GGCGCC               GG^CGCC
PflMI        1821  CCAGACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         613  CACGTG               CAC^GTG
PmlI          613  CACGTG               CAC^GTG
PshBI        1543  ATTAAT               AT^TAAT
RcaI          261  TCATGA               T^CATGA
SacI          697  GAGCTC               GAGCT^C
SapI         2902  GCTCTTC              GCTCTTC(1/4)
SfoI         1869  GGCGCC               GGC^GCC
SphI         2681  GCATGC               GCATG^C
SspBI        2841  TGTACA               T^GTACA
SspI         2260  AATATT               AAT^ATT
Van91I       1821  CCAGACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         2089  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII       2394  ACATGT               A^C[GA][CT]GT
             2586  ACATGT              
BbsI         2668  GAAGAC               GAAGAC(2/6)
             3286  GAAGAC              
BbvCI         939  GCTGAGG              CCTCAGC(-5/-2)
             1356  GCTGAGG             
BpuAI        2668  GAAGAC               GAAGAC(2/6)
             3286  GAAGAC              
BsaAI         613  CACGTG               [CT]AC^GT[GA]
             3347  CACGTA              
BsgI         1897  GTGCAG               GTGCAG(16/14)
             2921  GTGCAG              
BspLU11I     2394  ACATGT               A^CATGT
             2586  ACATGT              
BspMI        1749  ACCTGC               ACCTGC(4/8)
             2706  GCAGGT              
BstAPI        453  GCAACAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1890  GCAGGCTGTGC         
EarI         2903  CTCTTC               CTCTTC(1/4)
              624  GAAGAG              
EcoNI         813  CCTCTATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2494  CCTTCTCCAGG         
FauI         2688  CCCGC                CCCGC(4/6)
             1473  GCGGG               
HaeII         479  AGCGCT               [GA]GCGC^[CT]
             1869  GGCGCC              
HgaI         1030  GCGTC                GACGC(5/10)
             1380  GCGTC               
HincII       1617  GTCAAC               GT[CT]^[GA]AC
             1908  GTTGAC              
HindII       1617  GTCAAC               GT[CT]^[GA]AC
             1908  GTTGAC              
Ksp632I      2903  CTCTTC               CTCTTC(1/4)
              624  GAAGAG              
MspA1I       1354  CCGCTG               C[AC]G^C[GT]G
             3065  CCGCTG              
NcoI         1634  CCATGG               C^CATGG
             2300  CCATGG              
PpuMI        1059  AGGTCCC              [GA]G^G[AT]CC[CT]
             2244  GGGTCCT             
SmlI          751  CTCAAG               C^T[CT][GA]AG
             1098  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BanII       BbvI        BcnI        BfaI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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