Restriction map for pF1KB4032
 (Linear) (Six-base) MAPSORT of: KIBB4032.seq  Check: 7115  from: 1  to: 3402



KIBB4032.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1758    3402 
   Size:       1758    1644
 
AccI GT'mk_AC

Cuts at:      0    1078    1406    1855    3402 
   Size:       1078     328     449    1547

  Fragments arranged by size: 

                1547    1078     449     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     394    1142    1565    1794    2048    2675    3402 
   Size:        176     218     748     423     229     254     627     727

  Fragments arranged by size: 

                 748     727     627     423     254     229     218     176
            
 
AfeI AGC'GCT

Cuts at:      0     481    3402 
   Size:        481    2921
 
AflIII A'CryG_T

Cuts at:      0    2394    2586    3402 
   Size:       2394     192     816

  Fragments arranged by size: 

                2394     816     192
 
AhdI GACnn_n'nnGTC

Cuts at:      0     786    3402 
   Size:        786    2616
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     542    3119    3129    3402 
   Size:        542    2577      10     273

  Fragments arranged by size: 

                2577     542     273      10
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1993    2025    3402 
   Size:       1993      32    1377

  Fragments arranged by size: 

                1993    1377      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     799    1185    2044    2056    2344    3402 
   Size:        799     386     859      12     288    1058

  Fragments arranged by size: 

                1058     859     799     386     288      12
 
ApaI G_GGCC'C

Cuts at:      0    2663    3402 
   Size:       2663     739
 
ApoI r'AATT_y

Cuts at:      0    2093    2785    2830    3402 
   Size:       2093     692      45     572

  Fragments arranged by size: 

                2093     692     572      45
 
AscI GG'CGCG_CC

Cuts at:      0    3316    3402 
   Size:       3316      86
 
AseI AT'TA_AT

Cuts at:      0    1544    3402 
   Size:       1544    1858
 
AvrII C'CTAG_G

Cuts at:      0     382    3402 
   Size:        382    3020
 
BamHI G'GATC_C

Cuts at:      0     309    3402 
   Size:        309    3093
 
BanI G'GyrC_C

Cuts at:      0    1869    3402 
   Size:       1869    1533
 
BanII G_rGCy'C

Cuts at:      0     701    2405    2663    3402 
   Size:        701    1704     258     739

  Fragments arranged by size: 

                1704     739     701     258
 
BbeI G_GCGC'C

Cuts at:      0    1873    3402 
   Size:       1873    1529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2675    3293    3402 
   Size:       2675     618     109

  Fragments arranged by size: 

                2675     618     109
 
BbvCI CC'TCA_GC

Cuts at:      0     940    1357    3402 
   Size:        940     417    2045

  Fragments arranged by size: 

                2045     940     417
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1659    1693    3402 
   Size:       1659      34    1709

  Fragments arranged by size: 

                1709    1659      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     275    1101    2658    3402 
   Size:        275     826    1557     744

  Fragments arranged by size: 

                1557     826     744     275
 
BglII A'GATC_T

Cuts at:      0     301    1198    1272    3402 
   Size:        301     897      74    2130

  Fragments arranged by size: 

                2130     897     301      74
 
BlpI GC'TnA_GC

Cuts at:      0     530     581     800    3402 
   Size:        530      51     219    2602

  Fragments arranged by size: 

                2602     530     219      51
 
Bme1580I G_kGCm'C

Cuts at:      0    2663    3402 
   Size:       2663     739
 
BmgBI CAC'GTC

Cuts at:      0     108    3402 
   Size:        108    3294
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1325    2362    3402 
   Size:       1325    1037    1040

  Fragments arranged by size: 

                1325    1040    1037
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2328    2436    3402 
   Size:       2328     108     966

  Fragments arranged by size: 

                2328     966     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1709    2483    2533    2626    3402 
   Size:         71    1638     774      50      93     776

  Fragments arranged by size: 

                1638     776     774      93      71      50
 
Bpu10I CC'TnA_GC

Cuts at:      0     940    1357    1481    3402 
   Size:        940     417     124    1921

  Fragments arranged by size: 

                1921     940     417     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736    1083    3402 
   Size:        736     347    2319

  Fragments arranged by size: 

                2319     736     347
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2299    2424    2715    3402 
   Size:       2299     125     291     687

  Fragments arranged by size: 

                2299     687     291     125
 
BsaAI yAC'GTr

Cuts at:      0     615    3349    3402 
   Size:        615    2734      53

  Fragments arranged by size: 

                2734     615      53
 
BsaBI GATnn'nnATC

Cuts at:      0    1010    3402 
   Size:       1010    2392
 
BsaHI Gr'CG_yC

Cuts at:      0    1870    3402 
   Size:       1870    1532
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1707    1737    3053    3083    3402 
   Size:       1707      30    1316      30     319

  Fragments arranged by size: 

                1707    1316     319      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2623    3402 
   Size:       2623     779
 
BseYI C'CCAG_C

Cuts at:      0     449    1019    1687    1778    3357    3402 
   Size:        449     570     668      91    1579      45

  Fragments arranged by size: 

                1579     668     570     449      91      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1918    2942    3402 
   Size:       1918    1024     460

  Fragments arranged by size: 

                1918    1024     460
 
BsiHKAI G_wGCw'C

Cuts at:      0     603     701    3148    3402 
   Size:        603      98    2447     254

  Fragments arranged by size: 

                2447     603     254      98
 
BsmI GAATG_Cn'

Cuts at:      0     864    1326    2466    3402 
   Size:        864     462    1140     936

  Fragments arranged by size: 

                1140     936     864     462
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      98    3402 
   Size:         98    3304
 
Bsp1286I G_dGCh'C

Cuts at:      0     603     701    2405    2663    3148    3402 
   Size:        603      98    1704     258     485     254

  Fragments arranged by size: 

                1704     603     485     258     254      98
 
BspHI T'CATG_A

Cuts at:      0     261    3402 
   Size:        261    3141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1758    2697    3402 
   Size:       1758     939     705

  Fragments arranged by size: 

                1758     939     705
 
BsrDI GCAATG_nn'

Cuts at:      0     642    3402 
   Size:        642    2760
 
BsrFI r'CCGG_y

Cuts at:      0     947    3402 
   Size:        947    2455
 
BsrGI T'GTAC_A

Cuts at:      0    2841    3402 
   Size:       2841     561
 
BssHII G'CGCG_C

Cuts at:      0    3316    3402 
   Size:       3316      86
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     459    1896    3402 
   Size:        459    1437    1506

  Fragments arranged by size: 

                1506    1437     459
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1243    3402 
   Size:       1243    2159
 
BstYI r'GATC_y

Cuts at:      0     114     301     309     390    1198    1272    3402 
   Size:        114     187       8      81     808      74    2130

  Fragments arranged by size: 

                2130     808     187     114      81      74       8
 
BstZ17I GTA'TAC

Cuts at:      0    1856    3402 
   Size:       1856    1546
 
Bsu36I CC'TnA_GG

Cuts at:      0    2519    3402 
   Size:       2519     883
 
BtgI C'CryG_G

Cuts at:      0    1634    1826    2300    3402 
   Size:       1634     192     474    1102

  Fragments arranged by size: 

                1634    1102     474     192
 
BtsI GCAGTG_nn'

Cuts at:      0     800    1183    1464    3402 
   Size:        800     383     281    1938

  Fragments arranged by size: 

                1938     800     383     281
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1028    1063    3335    3370    3402 
   Size:       1028      35    2272      35      32

  Fragments arranged by size: 

                2272    1028      35      35      32
 
DraI TTT'AAA

Cuts at:      0     320    3402 
   Size:        320    3082
 
DraIII CAC_nnn'GTG

Cuts at:      0    3143    3402 
   Size:       3143     259
 
EaeI y'GGCC_r

Cuts at:      0    1704    2101    2297    3402 
   Size:       1704     397     196    1105

  Fragments arranged by size: 

                1704    1105     397     196
 
EarI CTCTTCn'nnn_

Cuts at:      0     619    2909    3402 
   Size:        619    2290     493

  Fragments arranged by size: 

                2290     619     493
 
EcoICRI GAG'CTC

Cuts at:      0     699    3402 
   Size:        699    2703
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     176     394    1142    1565    1709    1794    2048 
   Size:         71     105     218     748     423     144      85     254

Cuts at:   2048    2483    2533    2626    2675    3402 
   Size:        435      50      93      49     727

  Fragments arranged by size: 

                 748     727     435     423     254     218     144     105
                  93      85      71      50      49
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     817    2498    3402 
   Size:        817    1681     904

  Fragments arranged by size: 

                1681     904     817
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1060    2245    2659    3402 
   Size:       1060    1185     414     743

  Fragments arranged by size: 

                1185    1060     743     414
 
EcoRI G'AATT_C

Cuts at:      0    2093    3402 
   Size:       2093    1309
 
EcoRV GAT'ATC

Cuts at:      0    1467    3402 
   Size:       1467    1935
 
HaeII r_GCGC'y

Cuts at:      0     483    1873    3402 
   Size:        483    1390    1529

  Fragments arranged by size: 

                1529    1390     483
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     772     804    1707    1739    1902    1934    3402 
   Size:        772      32     903      32     163      32    1468

  Fragments arranged by size: 

                1468     903     772     163      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1619    1910    3402 
   Size:       1619     291    1492

  Fragments arranged by size: 

                1619    1492     291
 
HindIII A'AGCT_T

Cuts at:      0    2269    3402 
   Size:       2269    1133
 
KasI G'GCGC_C

Cuts at:      0    1869    3402 
   Size:       1869    1533
 
MfeI C'AATT_G

Cuts at:      0     457    3402 
   Size:        457    2945
 
MscI TGG'CCA

Cuts at:      0    1706    3402 
   Size:       1706    1696
 
MspA1I CmG'CkG

Cuts at:      0    1356    3067    3402 
   Size:       1356    1711     335

  Fragments arranged by size: 

                1711    1356     335
 
NarI GG'CG_CC

Cuts at:      0    1870    3402 
   Size:       1870    1532
 
NcoI C'CATG_G

Cuts at:      0    1634    2300    3402 
   Size:       1634     666    1102

  Fragments arranged by size: 

                1634    1102     666
 
NspI r_CATG'y

Cuts at:      0    2398    2487    2590    2685    3096    3402 
   Size:       2398      89     103      95     411     306

  Fragments arranged by size: 

                2398     411     306     103      95      89
 
PciI A'CATG_T

Cuts at:      0    2394    2586    3402 
   Size:       2394     192     816

  Fragments arranged by size: 

                2394     816     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1827    3402 
   Size:       1827    1575
 
PmlI CAC'GTG

Cuts at:      0     615    3402 
   Size:        615    2787
 
PpuMI rG'GwC_Cy

Cuts at:      0    1060    2245    3402 
   Size:       1060    1185    1157

  Fragments arranged by size: 

                1185    1157    1060
 
PspOMI G'GGCC_C

Cuts at:      0    2659    3402 
   Size:       2659     743
 
PstI C_TGCA'G

Cuts at:      0     795     830    1056    3108    3402 
   Size:        795      35     226    2052     294

  Fragments arranged by size: 

                2052     795     294     226      35
 
SacI G_AGCT'C

Cuts at:      0     701    3402 
   Size:        701    2701
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2909    3402 
   Size:       2909     493
 
SfcI C'TryA_G

Cuts at:      0     791     826     973    1052    3084    3104    3402 
   Size:        791      35     147      79    2032      20     298

  Fragments arranged by size: 

                2032     791     298     147      79      35      20
 
SfoI GGC'GCC

Cuts at:      0    1871    3402 
   Size:       1871    1531
 
SmlI C'TyrA_G

Cuts at:      0     751    1098    3402 
   Size:        751     347    2304

  Fragments arranged by size: 

                2304     751     347
 
SphI G_CATG'C

Cuts at:      0    2685    3402 
   Size:       2685     717
 
SspI AAT'ATT

Cuts at:      0    2262    3402 
   Size:       2262    1140
 
StuI AGG'CCT

Cuts at:      0    1104    2208    2504    3402 
   Size:       1104    1104     296     898

  Fragments arranged by size: 

                1104    1104     898     296
 
StyI C'CwwG_G

Cuts at:      0     382    1142    1287    1634    2203    2300    3402 
   Size:        382     760     145     347     569      97    1102

  Fragments arranged by size: 

                1102     760     569     382     347     145      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2009    3402 
   Size:       2009    1393
 
TatI w'GTAC_w

Cuts at:      0    2467    2841    3402 
   Size:       2467     374     561

  Fragments arranged by size: 

                2467     561     374
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      60      92     937     969    1785    1817    2765    2797 
   Size:         60      32     845      32     816      32     948      32

Cuts at:   2797    3053    3085    3402 
   Size:        256      32     317

  Fragments arranged by size: 

                 948     845     816     317     256      60      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2026    2298    3157    3402 
   Size:       2026     272     859     245

  Fragments arranged by size: 

                2026     859     272     245
 
XmnI GAAnn'nnTTC

Cuts at:      0    2093    3402 
   Size:       2093    1309
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AlfI     AloI
    AlwNI     ApaI     ApoI     AscI     AseI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII  HindIII     KasI
     MfeI     MscI   MspA1I     NarI     NcoI     NspI     PciI    PflMI
     PmlI    PpuMI   PspOMI     PstI     SacI     SapI     SfcI     SfoI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AleI    ApaLI    AsiSI
     AvaI     BaeI    BciVI     BclI    BfrBI     BmtI    BsaWI    BsiEI
    BsiWI    BspEI    BsrBI    BssSI    BstBI   BstEII    BtgZI     ClaI
     DrdI     EagI     EciI     FalI     FseI     FspI    FspAI     HpaI
     KpnI     MluI     MmeI     MslI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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