Restriction map for pF1KB3954
 (Linear) (Six-base) MAPSORT of: KIBB3954.seq  Check: 5541  from: 1  to: 4398



KIBB3954.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     940    1282    4398 
   Size:        940     342    3116

  Fragments arranged by size: 

                3116     940     342
 
AccI GT'mk_AC

Cuts at:      0     702    1683    4398 
   Size:        702     981    2715

  Fragments arranged by size: 

                2715     981     702
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    4058    4189    4226    4398 
   Size:        617    3441     131      37     172

  Fragments arranged by size: 

                3441     617     172     131      37
 
AflIII A'CryG_T

Cuts at:      0    3972    4398 
   Size:       3972     426
 
AleI CACnn'nnGTG

Cuts at:      0    1130    4398 
   Size:       1130    3268
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1441    1540    2251    2422    2719    3367    3403    4398 
   Size:       1441      99     711     171     297     648      36     995

  Fragments arranged by size: 

                1441     995     711     648     297     171      99      36
            
 
ApaI G_GGCC'C

Cuts at:      0    1550    2073    2711    2818    4398 
   Size:       1550     523     638     107    1580

  Fragments arranged by size: 

                1580    1550     638     523     107
 
ApaLI G'TGCA_C

Cuts at:      0    1997    4398 
   Size:       1997    2401
 
ApoI r'AATT_y

Cuts at:      0     233    3796    4195    4219    4363    4398 
   Size:        233    3563     399      24     144      35

  Fragments arranged by size: 

                3563     399     233     144      35      24
 
AseI AT'TA_AT

Cuts at:      0    1163    4398 
   Size:       1163    3235
 
AvaI C'yCGr_G

Cuts at:      0     956     974    1235    1712    3242    3494    4398 
   Size:        956      18     261     477    1530     252     904

  Fragments arranged by size: 

                1530     956     904     477     261     252      18
 
AvrII C'CTAG_G

Cuts at:      0    2899    4398 
   Size:       2899    1499
 
BamHI G'GATC_C

Cuts at:      0     172    1033    4398 
   Size:        172     861    3365

  Fragments arranged by size: 

                3365     861     172
 
BanI G'GyrC_C

Cuts at:      0    1087    1312    1456    2032    2347    2806    3121    3346 
   Size:       1087     225     144     576     315     459     315     225

Cuts at:   3346    3571    4398 
   Size:        225     827

  Fragments arranged by size: 

                1087     827     576     459     315     315     225     225
                 225     144
 
BanII G_rGCy'C

Cuts at:      0     885     912    1218    1236    1328    1550    2010    2073 
   Size:        885      27     306      18      92     222     460      63

Cuts at:   2073    2487    2711    2818    2829    3529    4398 
   Size:        414     224     107      11     700     869

  Fragments arranged by size: 

                 885     869     700     460     414     306     224     222
                 107      92      63      27      18      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     856    2629    2989    3052    4398 
   Size:        856    1773     360      63    1346

  Fragments arranged by size: 

                1773    1346     856     360      63
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     586     758    4398 
   Size:        586     172    3640

  Fragments arranged by size: 

                3640     586     172
 
BclI T'GATC_A

Cuts at:      0    4356    4398 
   Size:       4356      42
 
BfrBI ATG'CAT

Cuts at:      0    3905    4398 
   Size:       3905     493
 
BglI GCCn_nnn'nGGC

Cuts at:      0     536    1869    2318    3209    4398 
   Size:        536    1333     449     891    1189

  Fragments arranged by size: 

                1333    1189     891     536     449
 
Bme1580I G_kGCm'C

Cuts at:      0    1092    1461    1550    2001    2037    2073    2352    2711 
   Size:       1092     369      89     451      36      36     279     359

Cuts at:   2711    2811    2818    3126    3351    3576    4398 
   Size:        100       7     308     225     225     822

  Fragments arranged by size: 

                1092     822     451     369     359     308     279     225
                 225     100      89      36      36       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     453    1877    3425    3840    3902    4271    4398 
   Size:        453    1424    1548     415      62     369     127

  Fragments arranged by size: 

                1548    1424     453     415     369     127      62
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506     572     626     725     737    1229    1232    1430 
   Size:        506      66      54      99      12     492       3     198

Cuts at:   1430    1517    1538    1583    1610    1817    1931    1994    2024 
   Size:         87      21      45      27     207     114      63      30

Cuts at:   2024    2204    2348    2399    2537    2555    2678    2771    2813 
   Size:        180     144      51     138      18     123      93      42

Cuts at:   2813    2879    3344    4065    4258    4398 
   Size:         66     465     721     193     140

  Fragments arranged by size: 

                 721     506     492     465     207     198     193     180
                 144     140     138     123     114      99      93      87
                  66      66      63      54      51      45      42      30
                  27      21      18      12       3
 
Bpu10I CC'TnA_GC

Cuts at:      0    1046    4398 
   Size:       1046    3352
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1121    3245    3697    3799    4398 
   Size:        296     825    2124     452     102     599

  Fragments arranged by size: 

                2124     825     599     452     296     102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1076    2965    3782    4398 
   Size:       1076    1889     817     616

  Fragments arranged by size: 

                1889    1076     817     616
 
BsaAI yAC'GTr

Cuts at:      0    1336    3271    4398 
   Size:       1336    1935    1127

  Fragments arranged by size: 

                1935    1336    1127
 
BsaHI Gr'CG_yC

Cuts at:      0    1583    4398 
   Size:       1583    2815
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     276     306     345     375     479     509     822     852 
   Size:        276      30      39      30     104      30     313      30

Cuts at:    852    2397    2427    2721    2751    4398 
   Size:       1545      30     294      30    1647

  Fragments arranged by size: 

                1647    1545     313     294     276     104      39      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1118    1568    2207    3206    4398 
   Size:       1118     450     639     999    1192

  Fragments arranged by size: 

                1192    1118     999     639     450
 
BseYI C'CCAG_C

Cuts at:      0     760    2313    2683    2848    3616    4398 
   Size:        760    1553     370     165     768     782

  Fragments arranged by size: 

                1553     782     768     760     370     165
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1310    4075    4398 
   Size:        275    1035    2765     323

  Fragments arranged by size: 

                2765    1035     323     275
 
BsiHKAI G_wGCw'C

Cuts at:      0     840     885     957     975    1137    1218    1272    1533 
   Size:        840      45      72      18     162      81      54     261

Cuts at:   1533    1767    2001    2010    2442    2631    2739    2829    2865 
   Size:        234     234       9     432     189     108      90      36

Cuts at:   2865    3225    3252    4398 
   Size:        360      27    1146

  Fragments arranged by size: 

                1146     840     432     360     261     234     234     189
                 162     108      90      81      72      54      45      36
                  27      18       9
 
BsmI GAATG_Cn'

Cuts at:      0    1205    2183    4063    4398 
   Size:       1205     978    1880     335

  Fragments arranged by size: 

                1880    1205     978     335
 
Bsp1286I G_dGCh'C

Cuts at:      0     840     885     912     957     975    1092    1137    1218 
   Size:        840      45      27      45      18     117      45      81

Cuts at:   1218    1236    1272    1328    1461    1533    1550    1767    2001 
   Size:         18      36      56     133      72      17     217     234

Cuts at:   2001    2010    2037    2073    2352    2442    2487    2631    2711 
   Size:          9      27      36     279      90      45     144      80

Cuts at:   2711    2739    2811    2818    2829    2865    3126    3225    3252 
   Size:         28      72       7      11      36     261      99      27

Cuts at:   3252    3351    3529    3576    4398 
   Size:         99     178      47     822

  Fragments arranged by size: 

                 840     822     279     261     234     217     178     144
                 133     117      99      99      90      81      80      72
                  72      56      47      45      45      45      45      36
                  36      36      28      27      27      27      18      18
                  17      11       9       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     940    1071    1282    1485    2430    2556    3411    4398 
   Size:        940     131     211     203     945     126     855     987

  Fragments arranged by size: 

                 987     945     940     855     211     203     131     126
            
 
BsrDI GCAATG_nn'

Cuts at:      0    3611    4124    4398 
   Size:       3611     513     274

  Fragments arranged by size: 

                3611     513     274
 
BsrFI r'CCGG_y

Cuts at:      0    1255    1993    4398 
   Size:       1255     738    2405

  Fragments arranged by size: 

                2405    1255     738
 
BssSI C'ACGA_G

Cuts at:      0    2378    2630    4398 
   Size:       2378     252    1768

  Fragments arranged by size: 

                2378    1768     252
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2178    4398 
   Size:       2178    2220
 
BstEII G'GTnAC_C

Cuts at:      0     361    2662    4398 
   Size:        361    2301    1736

  Fragments arranged by size: 

                2301    1736     361
 
BstYI r'GATC_y

Cuts at:      0     172     327     583    1033    3076    3517    4398 
   Size:        172     155     256     450    2043     441     881

  Fragments arranged by size: 

                2043     881     450     441     256     172     155
 
Bsu36I CC'TnA_GG

Cuts at:      0    1118    1811    1847    2918    4398 
   Size:       1118     693      36    1071    1480

  Fragments arranged by size: 

                1480    1118    1071     693      36
 
BtgI C'CryG_G

Cuts at:      0     893    3931    3992    4398 
   Size:        893    3038      61     406

  Fragments arranged by size: 

                3038     893     406      61
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     200    4398 
   Size:        200    4198
 
BtsI GCAGTG_nn'

Cuts at:      0     987    4110    4398 
   Size:        987    3123     288

  Fragments arranged by size: 

                3123     987     288
 
DraIII CAC_nnn'GTG

Cuts at:      0    1339    2149    3274    4398 
   Size:       1339     810    1125    1124

  Fragments arranged by size: 

                1339    1125    1124     810
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     896    1904    2552    2678    2993    3470    4398 
   Size:        896    1008     648     126     315     477     928

  Fragments arranged by size: 

                1008     928     896     648     477     315     126
 
EaeI y'GGCC_r

Cuts at:      0     928    2319    2778    4398 
   Size:        928    1391     459    1620

  Fragments arranged by size: 

                1620    1391     928     459
 
EarI CTCTTCn'nnn_

Cuts at:      0    3027    4398 
   Size:       3027    1371
 
EcoICRI GAG'CTC

Cuts at:      0     883    1216    2008    2827    4398 
   Size:        883     333     792     819    1571

  Fragments arranged by size: 

                1571     883     819     792     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506     572     617     626     725     737    1229    1232 
   Size:        506      66      45       9      99      12     492       3

Cuts at:   1232    1430    1517    1538    1583    1610    1817    1931    1994 
   Size:        198      87      21      45      27     207     114      63

Cuts at:   1994    2024    2204    2348    2399    2537    2555    2678    2771 
   Size:         30     180     144      51     138      18     123      93

Cuts at:   2771    2813    2879    3344    4058    4065    4189    4226    4258 
   Size:         42      66     465     714       7     124      37      32

Cuts at:   4258    4398 
   Size:        140

  Fragments arranged by size: 

                 714     506     492     465     207     198     180     144
                 140     138     124     123     114      99      93      87
                  66      66      63      51      45      45      42      37
                  32      30      27      21      18      12       9       7
                   3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1934    3845    4398 
   Size:       1934    1911     553

  Fragments arranged by size: 

                1934    1911     553
 
EcoO109I rG'GnC_Cy

Cuts at:      0     307     316     334     389     538     601     637     646 
   Size:        307       9      18      55     149      63      36       9

Cuts at:    646     997    1141    1195    1240    1474    1510    1537    1546 
   Size:        351     144      54      45     234      36      27       9

Cuts at:   1546    1654    1690    1801    1816    1852    1861    1897    2069 
   Size:        108      36     111      15      36       9      36     172

Cuts at:   2069    2070    2284    2419    2509    2527    2671    2680    2708 
   Size:          1     214     135      90      18     144       9      28

Cuts at:   2708    2716    2814    2896    3364    3400    3409    3418    3472 
   Size:          8      98      82     468      36       9       9      54

Cuts at:   3472    3490    3553    4164    4398 
   Size:         18      63     611     234

  Fragments arranged by size: 

                 611     468     351     307     234     234     214     172
                 149     144     144     135     111     108      98      90
                  82      63      63      55      54      54      45      36
                  36      36      36      36      36      28      27      18
                  18      18      15       9       9       9       9       9
                   9       9       8       1
 
EcoRI G'AATT_C

Cuts at:      0    4195    4398 
   Size:       4195     203
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4063    4398 
   Size:       4063     335
 
HaeII r_GCGC'y

Cuts at:      0    3134    4398 
   Size:       3134    1264
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     164     189     196     466     498     679     711 
   Size:        157       7      25       7     270      32     181      32

Cuts at:    711    1568    1600    2782    2814    4398 
   Size:        857      32    1182      32    1584

  Fragments arranged by size: 

                1584    1182     857     270     181     157      32      32
                  32      32      25       7       7
 
HincII GTy'rAC

Cuts at:      0     991    1684    3837    4398 
   Size:        991     693    2153     561

  Fragments arranged by size: 

                2153     991     693     561
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     298    1038    3405    3579    4398 
   Size:        298     740    2367     174     819

  Fragments arranged by size: 

                2367     819     740     298     174
 
MscI TGG'CCA

Cuts at:      0    2321    2780    4398 
   Size:       2321     459    1618

  Fragments arranged by size: 

                2321    1618     459
 
MslI CAynn'nnrTG

Cuts at:      0    1130    4398 
   Size:       1130    3268
 
MspA1I CmG'CkG

Cuts at:      0     526     760    1201    2317    2687    3019    3172    3607 
   Size:        526     234     441    1116     370     332     153     435

Cuts at:   3607    4059    4398 
   Size:        452     339

  Fragments arranged by size: 

                1116     526     452     441     435     370     339     332
                 234     153
 
NcoI C'CATG_G

Cuts at:      0     893    3992    4398 
   Size:        893    3099     406

  Fragments arranged by size: 

                3099     893     406
 
NdeI CA'TA_TG

Cuts at:      0     471    4398 
   Size:        471    3927
 
NsiI A_TGCA'T

Cuts at:      0    3907    4398 
   Size:       3907     491
 
NspI r_CATG'y

Cuts at:      0    1614    2892    3905    4398 
   Size:       1614    1278    1013     493

  Fragments arranged by size: 

                1614    1278    1013     493
 
PasI CC'CwG_GG

Cuts at:      0    1462    1858    2263    2811    2991    3541    4398 
   Size:       1462     396     405     548     180     550     857

  Fragments arranged by size: 

                1462     857     550     548     405     396     180
 
PfoI T'CCnGG_A

Cuts at:      0     375     516     588     642     840     885    1011    1209 
   Size:        375     141      72      54     198      45     126     198

Cuts at:   1209    1218    1245    1272    1371    1434    1596    1623    1659 
   Size:          9      27      27      99      63     162      27      36

Cuts at:   1659    1821    1947    2010    2055    2415    2442    2514    3396 
   Size:        162     126      63      45     360      27      72     882

Cuts at:   3396    3558    4398 
   Size:        162     840

  Fragments arranged by size: 

                 882     840     375     360     198     198     162     162
                 162     141     126     126      99      72      72      63
                  63      54      45      45      36      27      27      27
                  27       9
 
PmlI CAC'GTG

Cuts at:      0    1336    3271    4398 
   Size:       1336    1935    1127

  Fragments arranged by size: 

                1935    1336    1127
 
PpuMI rG'GwC_Cy

Cuts at:      0     307     389     601     646    1141    1195    1240    1474 
   Size:        307      82     212      45     495      54      45     234

Cuts at:   1474    1510    1690    1801    1816    1852    1897    2284    2419 
   Size:         36     180     111      15      36      45     387     135

Cuts at:   2419    2527    2671    2680    2716    2896    3400    3418    3472 
   Size:        108     144       9      36     180     504      18      54

Cuts at:   3472    4398 
   Size:        926

  Fragments arranged by size: 

                 926     504     495     387     307     234     212     180
                 180     144     135     111     108      82      54      54
                  45      45      45      36      36      36      18      15
                   9
 
PspOMI G'GGCC_C

Cuts at:      0    1546    2069    2707    2814    4398 
   Size:       1546     523     638     107    1584

  Fragments arranged by size: 

                1584    1546     638     523     107
 
PspXI vC'TCGA_Gb

Cuts at:      0     974    3494    4398 
   Size:        974    2520     904

  Fragments arranged by size: 

                2520     974     904
 
PstI C_TGCA'G

Cuts at:      0     951    1068    1446    1545    3408    3787    4398 
   Size:        951     117     378      99    1863     379     611

  Fragments arranged by size: 

                1863     951     611     379     378     117      99
 
PvuII CAG'CTG

Cuts at:      0     526     760    2317    3019    3172    4059    4398 
   Size:        526     234    1557     702     153     887     339

  Fragments arranged by size: 

                1557     887     702     526     339     234     153
 
SacI G_AGCT'C

Cuts at:      0     885    1218    2010    2829    4398 
   Size:        885     333     792     819    1569

  Fragments arranged by size: 

                1569     885     819     792     333
 
SalI G'TCGA_C

Cuts at:      0    1682    4398 
   Size:       1682    2716
 
SanDI GG'GwC_CC

Cuts at:      0     389    1195    1852    4398 
   Size:        389     806     657    2546

  Fragments arranged by size: 

                2546     806     657     389
 
SbfI CC_TGCA'GG

Cuts at:      0    1068    1545    3408    4398 
   Size:       1068     477    1863     990

  Fragments arranged by size: 

                1863    1068     990     477
 
SexAI A'CCwGG_T

Cuts at:      0    1416    1686    1974    2145    2460    2604    3153    4398 
   Size:       1416     270     288     171     315     144     549    1245

  Fragments arranged by size: 

                1416    1245     549     315     288     270     171     144
            
 
SfcI C'TryA_G

Cuts at:      0     659     947    1064    1442    1541    3404    3783    4398 
   Size:        659     288     117     378      99    1863     379     615

  Fragments arranged by size: 

                1863     659     615     379     378     288     117      99
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     536    1869    2318    3209    4398 
   Size:        536    1333     449     891    1189

  Fragments arranged by size: 

                1333    1189     891     536     449
 
SmaI CCC'GGG

Cuts at:      0    1237    4398 
   Size:       1237    3161
 
SmlI C'TyrA_G

Cuts at:      0     311     974    1136    3260    3494    3712    3814    4398 
   Size:        311     663     162    2124     234     218     102     584

  Fragments arranged by size: 

                2124     663     584     311     234     218     162     102
            
 
SphI G_CATG'C

Cuts at:      0    1614    2892    4398 
   Size:       1614    1278    1506

  Fragments arranged by size: 

                1614    1506    1278
 
StyI C'CwwG_G

Cuts at:      0     320     597     893    2278    2568    2899    3846    3992 
   Size:        320     277     296    1385     290     331     947     146

Cuts at:   3992    4398 
   Size:        406

  Fragments arranged by size: 

                1385     947     406     331     320     296     290     277
                 146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3154    3554    4398 
   Size:       3154     400     844

  Fragments arranged by size: 

                3154     844     400
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     345     377    2721    2753    3612    3644    4398 
   Size:        345      32    2344      32     859      32     754

  Fragments arranged by size: 

                2344     859     754     345      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1688    4398 
   Size:       1688    2710
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     604    2998    3938    4398 
   Size:         60     544    2394     940     460

  Fragments arranged by size: 

                2394     940     544     460      60
 
XhoI C'TCGA_G

Cuts at:      0     974    3494    4398 
   Size:        974    2520     904

  Fragments arranged by size: 

                2520     974     904
 
XmaI C'CCGG_G

Cuts at:      0    1235    4398 
   Size:       1235    3163
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BciVI     BclI    BfrBI     BglI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NsiI     NspI     PasI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI     SalI    SanDI     SbfI    SexAI
     SfcI     SfiI     SmaI     SmlI     SphI     StyI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     AscI    AsiSI     BaeI     BbeI    BbvCI     BcgI    BglII
     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BstBI    BstXI
  BstZ17I     ClaI    CspCI     DraI     EagI     EciI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII
    SacII     SapI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XmnI     ZraI
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