Restriction map for pF1KB3179
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AspEI       AvaI        
AviII       AvrII       BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlnI        BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      BssSI       BstEII      
BstPI       BstUI       CfoI        ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HpaI        KasI        
KspI        MluI        MroI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       3385  GGTACC               G^GTACC
AseI         3030  ATTAAT               AT^TAAT
AsnI         3030  ATTAAT               AT^TAAT
Asp718I      3385  GGTACC               G^GTACC
BalI         1230  TGGCCA               TGG^CCA
BanI         3385  GGTACC               G^G[CT][GA]CC
BclI          831  TGATCA               T^GATCA
BlpI         2704  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2704  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2729  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1790  GAGGAG               GAGGAG(10/8)
BspLU11I     1613  ACATGT               A^CATGT
BsrFI        2915  ACCGGC               [GA]^CCGG[CT]
CelII        2704  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2915  ACCGGC               [GA]^CCGG[CT]
DraIII       2031  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1230  TGGCCA               [CT]^GGCC[GA]
EcoRV        2401  GATATC               GAT^ATC
EcoT22I      4289  ATGCAT               ATGCA^T
FauI         3382  GCGGG                CCCGC(4/6)
FbaI          831  TGATCA               T^GATCA
HgaI         3344  GACGC                GACGC(5/10)
KpnI         3385  GGTACC               GGTAC^C
MamI         2729  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1230  TGGCCA               TGG^CCA
MscI         1230  TGGCCA               TGG^CCA
NsiI         4289  ATGCAT               ATGCA^T
PshBI        3030  ATTAAT               AT^TAAT
PvuII        4539  CAGCTG               CAG^CTG
SacI         4168  GAGCTC               GAGCT^C
SapI         1918  GCTCTTC              GCTCTTC(1/4)
SexAI        1279  ACCAGGT              A^CC[AT]GGT
SwaI         4040  ATTTAAAT             ATTT^AAAT
XcmI         2690  CCAGATTAACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflIII        339  ACGTGT               A^C[GA][CT]GT
             1613  ACATGT              
AocI         1437  CCTAAGG              CC^T[ACGT]AGG
             3390  CCTGAGG             
ApaI         2479  GGGCCC               GGGCC^C
             4407  GGGCCC              
AspHI         755  GAGCAC               G[AT]GC[AT]^C
             4168  GAGCTC              
AspI         1690  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2902  GACTATGTC           
BamHI         680  GGATCC               G^GATCC
             1804  GGATCC              
BcnI         1420  CCGGG                CC^[GC]GG
             4410  CCCGG               
BglII        1683  AGATCT               A^GATCT
             2881  AGATCT              
BsaAI         338  TACGTG               [CT]AC^GT[GA]
             1600  CACGTA              
BsaI         3400  GGTCTC               GGTCTC(1/5)
             3963  GGTCTC              
BsiHKAI       755  GAGCAC               G[AT]GC[AT]^C
             4168  GAGCTC              
BspHI        1758  TCATGA               T^CATGA
             3924  TCATGA              
BstAPI       1738  GCAATGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1948  GCATGTTGTGC         
Bsu36I       1437  CCTAAGG              CC^T[ACGT]AGG
             3390  CCTGAGG             
DraI         2620  TTTAAA               TTT^AAA
             4041  TTTAAA              
DsaI         1233  CCATGG               C^C[GA][CT]GG
             1319  CCATGG              
Eco81I       1437  CCTAAGG              CC^T[ACGT]AGG
             3390  CCTGAGG             
EcoNI        1820  CCTGTTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4486  CCTTGGAAAGG         
HincII        916  GTCAAC               GT[CT]^[GA]AC
             2641  GTTGAC              
HindII        916  GTCAAC               GT[CT]^[GA]AC
             2641  GTTGAC              
MspA1I       3288  CCGCTG               C[AC]G^C[GT]G
             4539  CAGCTG              
NciI         1420  CCGGG                CC^[GC]GG
             4410  CCCGG               
NcoI         1233  CCATGG               C^CATGG
             1319  CCATGG              
NdeI          838  CATATG               CA^TATG
             3968  CATATG              
PflFI        1690  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2902  GACTATGTC           
PflMI         151  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3832  CCAAGAGATGG         
PleI          492  GACTC                GAGTC(4/5)
             1160  GACTC               
RcaI         1758  TCATGA               T^CATGA
             3924  TCATGA              
ScaI         1927  AGTACT               AGT^ACT
             4526  AGTACT              
SspI         3364  AATATT               AAT^ATT
             3910  AATATT              
StuI         1828  AGGCCT               AGG^CCT
             4132  AGGCCT              
Tth111I      1690  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2902  GACTATGTC           
Van91I        151  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3832  CCAAGAGATGG         
XbaI         2239  TCTAGA               T^CTAGA
             3513  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaII       BanII       
BbsI        BbvI        BciVI       BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       Bst1107I    BstBI       
BstF5I      BstNI       BstXI       BstYI       BstZ17I     
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MflI        MnlI        MseI        MslI        
MspI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        NspV        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfuI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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