Restriction map for pF1KB3179
 (Linear) (Six-base) MAPSORT of: KIBB3179.seq  Check: 5889  from: 1  to: 4596



KIBB3179.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3189    4596 
   Size:       3189    1407
 
AccI GT'mk_AC

Cuts at:      0     139     574     650    3327    4596 
   Size:        139     435      76    2677    1269

  Fragments arranged by size: 

                2677    1269     435     139      76
 
Acc65I G'GTAC_C

Cuts at:      0    3385    4596 
   Size:       3385    1211
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     370    2036    2066    2966    3004    4019    4596 
   Size:         51     319    1666      30     900      38    1015     577

  Fragments arranged by size: 

                1666    1015     900     577     319      51      38      30
            
 
AflIII A'CryG_T

Cuts at:      0     339    1613    4596 
   Size:        339    1274    2983

  Fragments arranged by size: 

                2983    1274     339
 
AleI CACnn'nnGTG

Cuts at:      0    4073    4596 
   Size:       4073     523
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1209    2302    4596 
   Size:       1209    1093    2294

  Fragments arranged by size: 

                2294    1209    1093
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1796    1828    4596 
   Size:       1796      32    2768

  Fragments arranged by size: 

                2768    1796      32
 
ApaI G_GGCC'C

Cuts at:      0    2483    4411    4596 
   Size:       2483    1928     185

  Fragments arranged by size: 

                2483    1928     185
 
ApoI r'AATT_y

Cuts at:      0      23     181     717     843     898    1143    1632    2379 
   Size:         23     158     536     126      55     245     489     747

Cuts at:   2379    2506    2582    2666    3154    3428    4277    4596 
   Size:        127      76      84     488     274     849     319

  Fragments arranged by size: 

                 849     747     536     489     488     319     274     245
                 158     127     126      84      76      55      23
 
AseI AT'TA_AT

Cuts at:      0    3031    4596 
   Size:       3031    1565
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3432    3465    4596 
   Size:       3432      33    1131

  Fragments arranged by size: 

                3432    1131      33
 
BamHI G'GATC_C

Cuts at:      0     680    1804    4596 
   Size:        680    1124    2792

  Fragments arranged by size: 

                2792    1124     680
 
BanI G'GyrC_C

Cuts at:      0    3385    4596 
   Size:       3385    1211
 
BanII G_rGCy'C

Cuts at:      0    2483    4172    4411    4596 
   Size:       2483    1689     239     185

  Fragments arranged by size: 

                2483    1689     239     185
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     948    2186    2797    4034    4596 
   Size:        948    1238     611    1237     562

  Fragments arranged by size: 

                1238    1237     948     611     562
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3051    3297    3490    3557    4596 
   Size:       3051     246     193      67    1039

  Fragments arranged by size: 

                3051    1039     246     193      67
 
BclI T'GATC_A

Cuts at:      0     831    4596 
   Size:        831    3765
 
BfrBI ATG'CAT

Cuts at:      0    4291    4596 
   Size:       4291     305
 
BglII A'GATC_T

Cuts at:      0    1683    2881    4596 
   Size:       1683    1198    1715

  Fragments arranged by size: 

                1715    1683    1198
 
BlpI GC'TnA_GC

Cuts at:      0    2705    4596 
   Size:       2705    1891
 
Bme1580I G_kGCm'C

Cuts at:      0    1521    2483    3532    4411    4596 
   Size:       1521     962    1049     879     185

  Fragments arranged by size: 

                1521    1049     962     879     185
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1720    3427    3526    4518    4596 
   Size:       1720    1707      99     992      78

  Fragments arranged by size: 

                1720    1707     992      99      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179    2483    2507    2673    2906    4596 
   Size:        179    2304      24     166     233    1690

  Fragments arranged by size: 

                2304    1690     233     179     166      24
 
Bpu10I CC'TnA_GC

Cuts at:      0    4129    4166    4596 
   Size:       4129      37     430

  Fragments arranged by size: 

                4129     430      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3508    4495    4596 
   Size:       3508     987     101

  Fragments arranged by size: 

                3508     987     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3406    3969    4596 
   Size:       3406     563     627

  Fragments arranged by size: 

                3406     627     563
 
BsaAI yAC'GTr

Cuts at:      0     340    1602    4596 
   Size:        340    1262    2994

  Fragments arranged by size: 

                2994    1262     340
 
BsaBI GATnn'nnATC

Cuts at:      0    2733    4596 
   Size:       2733    1863
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1454    1484    1784    1814    4596 
   Size:       1454      30     300      30    2782

  Fragments arranged by size: 

                2782    1454     300      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1805    4596 
   Size:       1805    2791
 
BseYI C'CCAG_C

Cuts at:      0    1457    3293    3822    4596 
   Size:       1457    1836     529     774

  Fragments arranged by size: 

                1836    1457     774     529
 
BsiHKAI G_wGCw'C

Cuts at:      0     759    4172    4596 
   Size:        759    3413     424

  Fragments arranged by size: 

                3413     759     424
 
BsmI GAATG_Cn'

Cuts at:      0    1111    3666    4524    4596 
   Size:       1111    2555     858      72

  Fragments arranged by size: 

                2555    1111     858      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     759    1521    2483    3532    4172    4411    4596 
   Size:        759     762     962    1049     640     239     185

  Fragments arranged by size: 

                1049     962     762     759     640     239     185
 
BspHI T'CATG_A

Cuts at:      0    1758    3924    4596 
   Size:       1758    2166     672

  Fragments arranged by size: 

                2166    1758     672
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     744     822    2791    3189    4596 
   Size:        744      78    1969     398    1407

  Fragments arranged by size: 

                1969    1407     744     398      78
 
BsrDI GCAATG_nn'

Cuts at:      0     484    1052    1745    2050    3241    3626    4157    4596 
   Size:        484     568     693     305    1191     385     531     439

  Fragments arranged by size: 

                1191     693     568     531     484     439     385     305
            
 
BsrFI r'CCGG_y

Cuts at:      0    2915    4596 
   Size:       2915    1681
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1744    1954    4596 
   Size:       1744     210    2642

  Fragments arranged by size: 

                2642    1744     210
 
BstBI TT'CG_AA

Cuts at:      0      21    1404    2589    4596 
   Size:         21    1383    1185    2007

  Fragments arranged by size: 

                2007    1383    1185      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3054    4152    4404    4596 
   Size:       3054    1098     252     192

  Fragments arranged by size: 

                3054    1098     252     192
 
BstYI r'GATC_y

Cuts at:      0     680    1011    1683    1804    2652    2717    2881    2990 
   Size:        680     331     672     121     848      65     164     109

Cuts at:   2990    4251    4480    4596 
   Size:       1261     229     116

  Fragments arranged by size: 

                1261     848     680     672     331     229     164     121
                 116     109      65
 
BstZ17I GTA'TAC

Cuts at:      0     140     575    3328    4596 
   Size:        140     435    2753    1268

  Fragments arranged by size: 

                2753    1268     435     140
 
Bsu36I CC'TnA_GG

Cuts at:      0    1438    3391    4596 
   Size:       1438    1953    1205

  Fragments arranged by size: 

                1953    1438    1205
 
BtgI C'CryG_G

Cuts at:      0    1233    1319    4596 
   Size:       1233      86    3277

  Fragments arranged by size: 

                3277    1233      86
 
BtsI GCAGTG_nn'

Cuts at:      0    3481    4596 
   Size:       3481    1115
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4139    4174    4596 
   Size:       4139      35     422

  Fragments arranged by size: 

                4139     422      35
 
DraI TTT'AAA

Cuts at:      0    2622    4043    4596 
   Size:       2622    1421     553

  Fragments arranged by size: 

                2622    1421     553
 
DraIII CAC_nnn'GTG

Cuts at:      0    2036    4596 
   Size:       2036    2560
 
EaeI y'GGCC_r

Cuts at:      0    1230    4596 
   Size:       1230    3366
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     389    1298    1406    1601    1757    1925    3056 
   Size:         70     319     909     108     195     156     168    1131

Cuts at:   3056    4596 
   Size:       1540

  Fragments arranged by size: 

                1540    1131     909     319     195     168     156     108
                  70
 
EcoICRI GAG'CTC

Cuts at:      0    4170    4596 
   Size:       4170     426
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     179     370    2036    2066    2483    2507    2673 
   Size:         51     128     191    1666      30     417      24     166

Cuts at:   2673    2906    2966    3004    4019    4596 
   Size:        233      60      38    1015     577

  Fragments arranged by size: 

                1666    1015     577     417     233     191     166     128
                  60      51      38      30      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1824    4490    4596 
   Size:       1824    2666     106

  Fragments arranged by size: 

                2666    1824     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     412    1249    2479    4342    4596 
   Size:        412     837    1230    1863     254

  Fragments arranged by size: 

                1863    1230     837     412     254
 
EcoRI G'AATT_C

Cuts at:      0     717     843    2379    2582    4596 
   Size:        717     126    1536     203    2014

  Fragments arranged by size: 

                2014    1536     717     203     126
 
EcoRV GAT'ATC

Cuts at:      0    2403    4596 
   Size:       2403    2193
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      81    2344    2868    3323    4596 
   Size:         81    2263     524     455    1273

  Fragments arranged by size: 

                2263    1273     524     455      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    3384    3416    4596 
   Size:       3384      32    1180

  Fragments arranged by size: 

                3384    1180      32
 
HincII GTy'rAC

Cuts at:      0     918    2643    4596 
   Size:        918    1725    1953

  Fragments arranged by size: 

                1953    1725     918
 
HindIII A'AGCT_T

Cuts at:      0     883    1352    3081    4196    4596 
   Size:        883     469    1729    1115     400

  Fragments arranged by size: 

                1729    1115     883     469     400
 
KpnI G_GTAC'C

Cuts at:      0    3389    4596 
   Size:       3389    1207
 
MfeI C'AATT_G

Cuts at:      0    1097    1934    3444    3762    4562    4596 
   Size:       1097     837    1510     318     800      34

  Fragments arranged by size: 

                1510    1097     837     800     318      34
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     570    1598    1782    2559    4596 
   Size:        232     338    1028     184     777    2037

  Fragments arranged by size: 

                2037    1028     777     338     232     184
 
MscI TGG'CCA

Cuts at:      0    1232    4596 
   Size:       1232    3364
 
MslI CAynn'nnrTG

Cuts at:      0     322    1880    3775    4073    4150    4596 
   Size:        322    1558    1895     298      77     446

  Fragments arranged by size: 

                1895    1558     446     322     298      77
 
MspA1I CmG'CkG

Cuts at:      0    3290    4541    4596 
   Size:       3290    1251      55

  Fragments arranged by size: 

                3290    1251      55
 
NcoI C'CATG_G

Cuts at:      0    1233    1319    4596 
   Size:       1233      86    3277

  Fragments arranged by size: 

                3277    1233      86
 
NdeI CA'TA_TG

Cuts at:      0     839    3969    4596 
   Size:        839    3130     627

  Fragments arranged by size: 

                3130     839     627
 
NsiI A_TGCA'T

Cuts at:      0    4293    4596 
   Size:       4293     303
 
NspI r_CATG'y

Cuts at:      0    1617    1855    1952    2566    3636    4596 
   Size:       1617     238      97     614    1070     960

  Fragments arranged by size: 

                1617    1070     960     614     238      97
 
PciI A'CATG_T

Cuts at:      0    1613    4596 
   Size:       1613    2983
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     157    3838    4596 
   Size:        157    3681     758

  Fragments arranged by size: 

                3681     758     157
 
PfoI T'CCnGG_A

Cuts at:      0     156     683    2523    3482    4596 
   Size:        156     527    1840     959    1114

  Fragments arranged by size: 

                1840    1114     959     527     156
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111    4596 
   Size:         79      32    4485

  Fragments arranged by size: 

                4485      79      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     412    1249    4342    4596 
   Size:        412     837    3093     254

  Fragments arranged by size: 

                3093     837     412     254
 
PspOMI G'GGCC_C

Cuts at:      0    2479    4407    4596 
   Size:       2479    1928     189

  Fragments arranged by size: 

                2479    1928     189
 
PstI C_TGCA'G

Cuts at:      0    3258    3476    3717    4596 
   Size:       3258     218     241     879

  Fragments arranged by size: 

                3258     879     241     218
 
PvuII CAG'CTG

Cuts at:      0    4541    4596 
   Size:       4541      55
 
SacI G_AGCT'C

Cuts at:      0    4172    4596 
   Size:       4172     424
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1925    4596 
   Size:       1925    2671
 
ScaI AGT'ACT

Cuts at:      0    1929    4528    4596 
   Size:       1929    2599      68

  Fragments arranged by size: 

                2599    1929      68
 
SexAI A'CCwGG_T

Cuts at:      0    1279    4596 
   Size:       1279    3317
 
SfcI C'TryA_G

Cuts at:      0      91    1040    3254    3472    3551    3713    4178    4430 
   Size:         91     949    2214     218      79     162     465     252

Cuts at:   4430    4596 
   Size:        166

  Fragments arranged by size: 

                2214     949     465     252     218     166     162      91
                  79
 
SmlI C'TyrA_G

Cuts at:      0    3523    4474    4576    4596 
   Size:       3523     951     102      20

  Fragments arranged by size: 

                3523     951     102      20
 
SspI AAT'ATT

Cuts at:      0    3366    3912    4596 
   Size:       3366     546     684

  Fragments arranged by size: 

                3366     684     546
 
StuI AGG'CCT

Cuts at:      0    1830    4134    4596 
   Size:       1830    2304     462

  Fragments arranged by size: 

                2304    1830     462
 
StyI C'CwwG_G

Cuts at:      0     368    1233    1319    2141    4111    4233    4338    4486 
   Size:        368     865      86     822    1970     122     105     148

Cuts at:   4486    4596 
   Size:        110

  Fragments arranged by size: 

                1970     865     822     368     148     122     110     105
                  86
 
SwaI ATTT'AAAT

Cuts at:      0    4043    4596 
   Size:       4043     553
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3409    4596 
   Size:       3409    1187
 
TatI w'GTAC_w

Cuts at:      0    1036    1610    1927    1966    4526    4596 
   Size:       1036     574     317      39    2560      70

  Fragments arranged by size: 

                2560    1036     574     317      70      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     584     616    2198    2230    3445    3477    4596 
   Size:        584      32    1582      32    1215      32    1119

  Fragments arranged by size: 

                1582    1215    1119     584      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1693    2905    4596 
   Size:       1693    1212    1691

  Fragments arranged by size: 

                1693    1691    1212
 
XbaI T'CTAG_A

Cuts at:      0    2239    3513    4596 
   Size:       2239    1274    1083

  Fragments arranged by size: 

                2239    1274    1083
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2697    4596 
   Size:       2697    1899
 
XmnI GAAnn'nnTTC

Cuts at:      0    2324    2426    2492    4596 
   Size:       2324     102      66    2104

  Fragments arranged by size: 

                2324    2104     102      66
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI
     ApaI     ApoI     AseI     BaeI    BamHI     BanI    BanII     BbsI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI     SapI     ScaI    SexAI     SfcI     SmlI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI    AlwNI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BbeI    BbvCI     BcgI     BglI
    BmgBI     BmtI     BplI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI   BstEII    BtgZI
     ClaI     DrdI     EagI     EciI     FseI     FspI    FspAI    HaeII
     HpaI     KasI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     XhoI     XmaI     ZraI
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