Restriction map for pF1KB3038
 (Linear) (Six-base) MAPSORT of: KIBB3038.seq  Check: 5295  from: 1  to: 4203



KIBB3038.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:09  ..

AccI GT'mk_AC

Cuts at:      0     416    1224    4203 
   Size:        416     808    2979

  Fragments arranged by size: 

                2979     808     416
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    2144    2444    2710    3530    3919    4203 
   Size:        281    1863     300     266     820     389     284

  Fragments arranged by size: 

                1863     820     389     300     284     281     266
 
AflII C'TTAA_G

Cuts at:      0    2929    4203 
   Size:       2929    1274
 
AhdI GACnn_n'nnGTC

Cuts at:      0     549    4203 
   Size:        549    3654
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1476    1615    2272    3670    4203 
   Size:       1476     139     657    1398     533

  Fragments arranged by size: 

                1476    1398     657     533     139
 
ApaLI G'TGCA_C

Cuts at:      0    4082    4203 
   Size:       4082     121
 
ApoI r'AATT_y

Cuts at:      0     503     617    1565    3558    4203 
   Size:        503     114     948    1993     645

  Fragments arranged by size: 

                1993     948     645     503     114
 
AseI AT'TA_AT

Cuts at:      0    2561    4203 
   Size:       2561    1642
 
AvrII C'CTAG_G

Cuts at:      0    1071    4203 
   Size:       1071    3132
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     345     378    4203 
   Size:        345      33    3825

  Fragments arranged by size: 

                3825     345      33
 
BamHI G'GATC_C

Cuts at:      0    3036    4203 
   Size:       3036    1167
 
BanI G'GyrC_C

Cuts at:      0     196    1306    2178    4203 
   Size:        196    1110     872    2025

  Fragments arranged by size: 

                2025    1110     872     196
 
BanII G_rGCy'C

Cuts at:      0    2593    2928    4203 
   Size:       2593     335    1275

  Fragments arranged by size: 

                2593    1275     335
 
BbeI G_GCGC'C

Cuts at:      0     200    4203 
   Size:        200    4003
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3877    4203 
   Size:       3877     326
 
BbvCI CC'TCA_GC

Cuts at:      0    2333    4203 
   Size:       2333    1870
 
BfrBI ATG'CAT

Cuts at:      0     433     756    4203 
   Size:        433     323    3447

  Fragments arranged by size: 

                3447     433     323
 
BglII A'GATC_T

Cuts at:      0     768    2604    3079    3594    4203 
   Size:        768    1836     475     515     609

  Fragments arranged by size: 

                1836     768     609     515     475
 
BlpI GC'TnA_GC

Cuts at:      0    2676    4203 
   Size:       2676    1527
 
Bme1580I G_kGCm'C

Cuts at:      0    4086    4203 
   Size:       4086     117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     226    1997    3042    3528    4203 
   Size:        226    1771    1045     486     675

  Fragments arranged by size: 

                1771    1045     675     486     226
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      37    1311    3941    4203 
   Size:         37    1274    2630     262

  Fragments arranged by size: 

                2630    1274     262      37
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1281    1358    1688    1990    2222    2942    3662    4203 
   Size:       1281      77     330     302     232     720     720     541

  Fragments arranged by size: 

                1281     720     720     541     330     302     232      77
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2333    4203 
   Size:       2333    1870
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1524    3852    4203 
   Size:       1524    2328     351

  Fragments arranged by size: 

                2328    1524     351
 
BsaAI yAC'GTr

Cuts at:      0    2911    4203 
   Size:       2911    1292
 
BsaHI Gr'CG_yC

Cuts at:      0     109     197    4203 
   Size:        109      88    4006

  Fragments arranged by size: 

                4006     109      88
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2469    2499    3103    3133    3635    3665    4161    4191 
   Size:       2469      30     604      30     502      30     496      30

Cuts at:   4191    4203 
   Size:         12

  Fragments arranged by size: 

                2469     604     502     496      30      30      30      30
                  12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     145    1273    1900    2088    3111    3231    3234 
   Size:        118      27    1128     627     188    1023     120       3

Cuts at:   3234    4169    4203 
   Size:        935      34

  Fragments arranged by size: 

                1128    1023     935     627     188     120     118      34
                  27       3
 
BseYI C'CCAG_C

Cuts at:      0     115     481    1752    1997    2412    2671    4203 
   Size:        115     366    1271     245     415     259    1532

  Fragments arranged by size: 

                1532    1271     415     366     259     245     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     582     698     971    1148    4203 
   Size:        582     116     273     177    3055

  Fragments arranged by size: 

                3055     582     273     177     116
 
BsiHKAI G_wGCw'C

Cuts at:      0    2928    4086    4203 
   Size:       2928    1158     117

  Fragments arranged by size: 

                2928    1158     117
 
BsmI GAATG_Cn'

Cuts at:      0     390    4203 
   Size:        390    3813
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1662    4203 
   Size:       1662    2541
 
Bsp1286I G_dGCh'C

Cuts at:      0    2593    2928    4086    4203 
   Size:       2593     335    1158     117

  Fragments arranged by size: 

                2593    1158     335     117
 
BspHI T'CATG_A

Cuts at:      0     186    4203 
   Size:        186    4017
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1835    1945    4203 
   Size:       1835     110    2258

  Fragments arranged by size: 

                2258    1835     110
 
BsrBI CCG'CTC

Cuts at:      0      92    4203 
   Size:         92    4111
 
BsrDI GCAATG_nn'

Cuts at:      0     629    3707    3910    3929    4068    4203 
   Size:        629    3078     203      19     139     135

  Fragments arranged by size: 

                3078     629     203     139     135      19
 
BsrGI T'GTAC_A

Cuts at:      0    1032    2878    4203 
   Size:       1032    1846    1325

  Fragments arranged by size: 

                1846    1325    1032
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     340    4203 
   Size:        340    3863
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2014    4203 
   Size:       2014    2189
 
BstYI r'GATC_y

Cuts at:      0     768     837    2604    3036    3079    3594    3852    4203 
   Size:        768      69    1767     432      43     515     258     351

  Fragments arranged by size: 

                1767     768     515     432     351     258      69      43
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1103    2115    4203 
   Size:       1103    1012    2088

  Fragments arranged by size: 

                2088    1103    1012
 
BtgI C'CryG_G

Cuts at:      0    1001    1044    1891    2289    3806    4203 
   Size:       1001      43     847     398    1517     397

  Fragments arranged by size: 

                1517    1001     847     398     397      43
 
BtsI GCAGTG_nn'

Cuts at:      0    1594    2105    2219    3761    4203 
   Size:       1594     511     114    1542     442

  Fragments arranged by size: 

                1594    1542     511     442     114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1329    1364    4203 
   Size:       1329      35    2839

  Fragments arranged by size: 

                2839    1329      35
 
DraI TTT'AAA

Cuts at:      0     560    1460    3237    4203 
   Size:        560     900    1777     966

  Fragments arranged by size: 

                1777     966     900     560
 
EaeI y'GGCC_r

Cuts at:      0     849    1047    1617    1700    4203 
   Size:        849     198     570      83    2503

  Fragments arranged by size: 

                2503     849     570     198      83
 
EarI CTCTTCn'nnn_

Cuts at:      0    1986    2454    4203 
   Size:       1986     468    1749

  Fragments arranged by size: 

                1986    1749     468
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     164    3978    4182    4203 
   Size:        164    3814     204      21

  Fragments arranged by size: 

                3814     204     164      21
 
EcoICRI GAG'CTC

Cuts at:      0    2926    4203 
   Size:       2926    1277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1281    1358    1688    1990    2144    2222    2444 
   Size:        281    1000      77     330     302     154      78     222

Cuts at:   2444    2710    2942    3530    3662    3919    4203 
   Size:        266     232     588     132     257     284

  Fragments arranged by size: 

                1000     588     330     302     284     281     266     257
                 232     222     154     132      78      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210    1957    3229    4143    4203 
   Size:        210    1747    1272     914      60

  Fragments arranged by size: 

                1747    1272     914     210      60
 
EcoRI G'AATT_C

Cuts at:      0    1565    4203 
   Size:       1565    2638
 
HaeII r_GCGC'y

Cuts at:      0     145     200    4203 
   Size:        145      55    4003

  Fragments arranged by size: 

                4003     145      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     535     567     645     677     690     722 
   Size:         94      32     409      32      78      32      13      32

Cuts at:    722    2287    2319    3175    3207    3635    3667    4203 
   Size:       1565      32     856      32     428      32     536

  Fragments arranged by size: 

                1565     856     536     428     409      94      78      32
                  32      32      32      32      32      32      13
 
HincII GTy'rAC

Cuts at:      0     585    2619    3465    4203 
   Size:        585    2034     846     738

  Fragments arranged by size: 

                2034     846     738     585
 
HindIII A'AGCT_T

Cuts at:      0     677    3859    4203 
   Size:        677    3182     344

  Fragments arranged by size: 

                3182     677     344
 
HpaI GTT'AAC

Cuts at:      0    2619    3465    4203 
   Size:       2619     846     738

  Fragments arranged by size: 

                2619     846     738
 
KasI G'GCGC_C

Cuts at:      0     196    4203 
   Size:        196    4007
 
MfeI C'AATT_G

Cuts at:      0    1036    1341    3092    4203 
   Size:       1036     305    1751    1111

  Fragments arranged by size: 

                1751    1111    1036     305
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    2723    4203 
   Size:        730    1993    1480

  Fragments arranged by size: 

                1993    1480     730
 
MscI TGG'CCA

Cuts at:      0     851    1049    1619    1702    4203 
   Size:        851     198     570      83    2501

  Fragments arranged by size: 

                2501     851     570     198      83
 
MslI CAynn'nnrTG

Cuts at:      0     191     428     438     641    1049    4203 
   Size:        191     237      10     203     408    3154

  Fragments arranged by size: 

                3154     408     237     203     191      10
 
MspA1I CmG'CkG

Cuts at:      0      37    1491    1585    2014    2416    4203 
   Size:         37    1454      94     429     402    1787

  Fragments arranged by size: 

                1787    1454     429     402      94      37
 
NarI GG'CG_CC

Cuts at:      0     197    4203 
   Size:        197    4006
 
NcoI C'CATG_G

Cuts at:      0    1001    1044    2289    3806    4203 
   Size:       1001      43    1245    1517     397

  Fragments arranged by size: 

                1517    1245    1001     397      43
 
NdeI CA'TA_TG

Cuts at:      0    2987    4203 
   Size:       2987    1216
 
NsiI A_TGCA'T

Cuts at:      0     435     758    4203 
   Size:        435     323    3445

  Fragments arranged by size: 

                3445     435     323
 
NspI r_CATG'y

Cuts at:      0    1360    2699    4203 
   Size:       1360    1339    1504

  Fragments arranged by size: 

                1504    1360    1339
 
PasI CC'CwG_GG

Cuts at:      0    1687    4203 
   Size:       1687    2516
 
PfoI T'CCnGG_A

Cuts at:      0    3128    3820    4203 
   Size:       3128     692     383

  Fragments arranged by size: 

                3128     692     383
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1961    1993    4203 
   Size:       1961      32    2210

  Fragments arranged by size: 

                2210    1961      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1957    3229    4143    4203 
   Size:       1957    1272     914      60

  Fragments arranged by size: 

                1957    1272     914      60
 
PsiI TTA'TAA

Cuts at:      0     428    4203 
   Size:        428    3775
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3794    3826    4203 
   Size:       3794      32     377

  Fragments arranged by size: 

                3794     377      32
 
PstI C_TGCA'G

Cuts at:      0    1059    1722    1832    3939    4203 
   Size:       1059     663     110    2107     264

  Fragments arranged by size: 

                2107    1059     663     264     110
 
PvuII CAG'CTG

Cuts at:      0    1491    1585    2014    2416    4203 
   Size:       1491      94     429     402    1787

  Fragments arranged by size: 

                1787    1491     429     402      94
 
SacI G_AGCT'C

Cuts at:      0    2928    4203 
   Size:       2928    1275
 
SexAI A'CCwGG_T

Cuts at:      0    2307    3429    4203 
   Size:       2307    1122     774

  Fragments arranged by size: 

                2307    1122     774
 
SfcI C'TryA_G

Cuts at:      0     357     764    1055    1225    1718    1734    1828    3935 
   Size:        357     407     291     170     493      16      94    2107

Cuts at:   3935    3990    4203 
   Size:         55     213

  Fragments arranged by size: 

                2107     493     407     357     291     213     170      94
                  55      16
 
SfoI GGC'GCC

Cuts at:      0     198    4203 
   Size:        198    4005
 
SmlI C'TyrA_G

Cuts at:      0    2929    4203 
   Size:       2929    1274
 
SphI G_CATG'C

Cuts at:      0    2699    4203 
   Size:       2699    1504
 
SspI AAT'ATT

Cuts at:      0    3261    3474    4203 
   Size:       3261     213     729

  Fragments arranged by size: 

                3261     729     213
 
StuI AGG'CCT

Cuts at:      0    1018    3118    4203 
   Size:       1018    2100    1085

  Fragments arranged by size: 

                2100    1085    1018
 
StyI C'CwwG_G

Cuts at:      0     497     791     827    1001    1044    1071    2289    3806 
   Size:        497     294      36     174      43      27    1218    1517

Cuts at:   3806    4203 
   Size:        397

  Fragments arranged by size: 

                1517    1218     497     397     294     174      43      36
                  27
 
SwaI ATTT'AAAT

Cuts at:      0    1460    4203 
   Size:       1460    2743
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2426    4203 
   Size:       2426    1777
 
TatI w'GTAC_w

Cuts at:      0    1032    1377    1759    2878    4065    4130    4203 
   Size:       1032     345     382    1119    1187      65      73

  Fragments arranged by size: 

                1187    1119    1032     382     345      73      65
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1346    1378    4203 
   Size:       1346      32    2825

  Fragments arranged by size: 

                2825    1346      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2395    4203 
   Size:       2395    1808
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2370    4203 
   Size:       2370    1833
 
XmnI GAAnn'nnTTC

Cuts at:      0    3535    4203 
   Size:       3535     668
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AhdI    AlwNI    ApaLI     ApoI     AseI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PasI
     PfoI     PpiI    PpuMI     PsiI     PsrI     PstI    PvuII     SacI
    SexAI     SfcI     SfoI     SmlI     SphI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI     ApaI     AscI    AsiSI     AvaI     BcgI    BciVI
     BclI     BglI    BmgBI     BmtI    BpuEI    BsaBI    BsaWI    BsiEI
    BsiWI    BspEI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI   DraIII     DrdI     EagI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI     KpnI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI    PflMI     PmeI     PmlI    PshAI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com